The β subunit of the Sec61 complex facilitates cotranslational protein transport and interacts with the signal peptidase during translocation

Journal of Cell Biology

Volumn 141, Issue 4, 1998, Pages 887-894

  Kalies, Kai Uwe ^a   Rapoport, Tom A ^b   Hartmann, Enno ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

^b HARVARD MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

PROTEIN SUBUNIT; SEC61 BETA; SIGNAL PEPTIDASE; UNCLASSIFIED DRUG;

ARTICLE; CELL MEMBRANE TRANSPORT; ENDOPLASMIC RETICULUM; IMMUNOBLOTTING; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN TRANSPORT; RIBOSOME; RNA TRANSLATION; SIGNAL TRANSDUCTION;

BINDING SITES; BIOLOGICAL TRANSPORT; CLONING, MOLECULAR; ENDOPLASMIC RETICULUM; GLYCOSYLATION; INTRACELLULAR MEMBRANES; KINETICS; LIPOSOMES; MACROMOLECULAR SUBSTANCES; MEMBRANE PROTEINS; MICROSOMES; MODELS, MOLECULAR; PEPTIDE CHAIN ELONGATION, TRANSLATIONAL; PROLACTIN; PROTEIN BIOSYNTHESIS; PROTEIN CONFORMATION; PROTEIN PRECURSORS; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEOLIPIDS; RIBOSOMES; SACCHAROMYCES CEREVISIAE; SACCHAROMYCES CEREVISIAE PROTEINS; SERINE ENDOPEPTIDASES; TRANSCRIPTION, GENETIC;

EID: 0031781639     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.141.4.887     Document Type: Article

References (21)

1. Beckmann, R., D. Bubeck, R. Grassucci, P. Penczek, A. Verschoor, G. Blobel, and J. Frank. 1997. Alignment of conduits for the nascent chain in the ribosome-Sec61 complex. Science. 278:2123-2126.
2. Blobel, G., and B. Dobberstein. 1975. Transfer of proteins across membranes. I. Presence of proteolytically processed and unprocessed nascent immunoglobulin light chains on membrane-bound ribosomes of murine myeloma. J. Cell Biol. 67:835-851.
3. Connolly, T., P. Collins, and R. Gilmore. 1989. Access of proteinase K to partially translocated nascent polypeptides in intact and detergent-solubilized membranes. J. Cell Biol. 108:299-307.
4. Crowley, K.S., G.D. Reinhart, and A.E. Johnson. 1993. The signal sequence moves through a ribosomal tunnel into a noncytoplasmic aqueous environment at the ER membrane early in translocation. Cell. 73:1101-1115.
5. Crowley, K.S., S.R. Liao, V.E. Worrell, G.D. Reinhart, and A.E. Johnson. 1994. Secretory proteins move through the endoplasmic reticulum membrane via an aqueous, gated pore. Cell. 78:461-471.
6. Evans, E.A., R. Gilmore, and G. Blobel. 1986. Purification of microsomal signal peptidase as a complex. Proc. Natl. Acad. Sci. USA. 83:581-585.
7. Fang, H., S. Panzner, C. Mullins, E. Hartmann, and N. Green. 1996. The homologue of mammalian SPC12 is important for efficient signal peptidase activity in Saccharomyces cerevisiae. J. Biol. Chem. 271:16460-16465.
8. Finke, K., K. Plath, S. Panzner, S. Prehn, T.A. Rapoport, E. Hartmann, and T. Sommer. 1996. A second trimeric complex containing homologs of the Sec61 complex functions in protein transport across the ER membrane of S. cerevisiae. EMBO (Eur. Mol. Biol. Organ.) J. 15:1482-1494.
9. Görlich, D., and T.A. Rapoport. 1993. Protein translocation into proteoliposomes reconstituted from purified components of the endoplasmic reticulum membrane. Cell. 75:615-630.
10. Görlich, D., E. Hartmann, S. Prehn, and T.A. Rapoport. 1992a. A protein of the endoplasmic reticulum involved early in polypeptide translocation. Nature. 357:47-52.
11. Görlich, D., S. Prehn, E. Hartmann, K.-U. Kalies and T.A. Rapoport. 1992b. A mammalian homolog of Sec61p and Secyp is associated with ribosomes and nascent polypeptides during translocation. Cell. 71:489-503.
12. Hanein, D., K.E.S. Matlack, B. Jungnickel, K. Plath, K.-U. Kalies, K.R. Miller, T.A. Rapoport, and C.W. Akey. 1996. Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation. Cell. 87:721-732.
13. Jungnickel, B., and T.A. Rapoport. 1995. A postlargeting signal sequence recognition event in the endoplasmic reticulum membrane. Cell. 82:261-270.
14. Kalies, K.-U., and E. Hartmann. 1996. Membrane topology of the 12-and the 25-kDa subunit of the mammalian signal peptidase complex. J. Biol. Chem. 271:3925-3929.
15. Kalies, K.-U., D. Görlich, and T.A. Rapoport. 1994. Binding of ribosomes to the rough endoplasmic reticulum mediated by the sec61p-complex. J. Cell Biol. 126:925-934.
16. Lively, M.O., and K.A. Walsh. 1983. Hen oviduct signal peptidase is an integral membrane protein. J. Biol. Chem. 258:9488-9495.
17. Mothes, W., S. Prehn, and T.A. Rapoport. 1994. Systematic probing of the environment of a translocating secretory protein during translocation through the ER membrane. EMBO (Eur. Mol. Biol. Organ.) J. 13:3937-3982.
18. Mullins, C., H.-A. Meyer, E. Hartmann, N. Green, and H. Fang. 1996. Structurally related Spclp and Spc2p of yeast signal peptidase complex are functionally distinct. J. Biol. Chem. 271:29094-29099.
19. Simon, S.M., and G. Blobel. 1991. A protein-conducting channel in the endoplasmic reticulum. Cell. 65:371-380.
20. Voigt, S., B. Jungnickel, E. Hartmann, and T.A. Rapoport. 1996. Signal sequence-dependent function of the TRAM protein during early phases of protein transport across the endoplasmic reticulum membrane. J. Cell Biol. 134:25-35.
21. Walter, P., and G. Blobel. 1983. Preparation of microsomal membranes for cotranslational protein translocation. Methods Enzymol. 96:84-93.