The aqueous pore thorough the translocon has a diameter of 40-60 Å during cotranslational protein translocation at the ER membrane

Cell

Volumn 89, Issue 4, 1997, Pages 535-544

  Hamman, Brian D ^a   Chen, Jui Chang ^a   Johnson, Edward E ^a   Johnson, Arthur E ^a  

^a TEXAS A AND M UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MEMBRANE PROTEIN; SECRETORY PROTEIN; 4 CHLORO 7 NITROBENZOFURAZAN; FLUORESCENT DYE; IMMUNOGLOBULIN F(AB) FRAGMENT; LYSINE TRANSFER RNA; NICOTINAMIDE ADENINE DINUCLEOTIDE; WATER;

ARTICLE; CYTOSOL; ENDOPLASMIC RETICULUM; FLUORESCENCE; MEMBRANE STRUCTURE; PRIORITY JOURNAL; PROTEIN TRANSPORT; RIBOSOME; ACTIVE TRANSPORT; ANIMAL; CHEMICAL STRUCTURE; CHEMISTRY; GENETICS; INTRACELLULAR MEMBRANE; LIPID BILAYER; METABOLISM; PERMEABILITY; PROTEIN CONFORMATION; PROTEIN SYNTHESIS; RABBIT; SACCHAROMYCES CEREVISIAE;

EUKARYOTA;

4-CHLORO-7-NITROBENZOFURAZAN; ANIMALS; BIOLOGICAL TRANSPORT, ACTIVE; CYTOSOL; ENDOPLASMIC RETICULUM; FLUORESCENT DYES; IMMUNOGLOBULIN FAB FRAGMENTS; INTRACELLULAR MEMBRANES; LIPID BILAYERS; MEMBRANE PROTEINS; MODELS, MOLECULAR; NAD; PERMEABILITY; PROTEIN BIOSYNTHESIS; PROTEIN CONFORMATION; RABBITS; RNA, TRANSFER, LYS; SACCHAROMYCES CEREVISIAE; WATER;

EID: 0030611388     PISSN: 00928674     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0092-8674(00)80235-4     Document Type: Article

References (37)

1. Amit, A.G., Mariuzza, R.A., Phillips, S.E., and Poljak, R.A. (1986). Three-dimensional structure of an antigen-antibody complex at 2.8 Å resolution. Science 233, 747-753.
2. Andrews, D.W., and Johnson, A.E. (1996).The translocon: more than a hole in the ER membrane? Trends Biochem. Sci. 21, 365-369.
3. Bell, C.E., and Eisenberg, D. (1996). Crystal structure of diphtheria toxin bound to nicotinamide adenine dinucleotide. Biochemistry 35, 1137-1149.
4. Blobel, G., and Sabatini, D.D. (1970). Controlled proteolysis of nascent polypeptides in rat liver cell fractions. I. Location of polypeptides within ribosomes. J. Cell Biol. 45, 130-145.
5. Borel, A.C., and Simon, S.M. (1996). Biogenesis of polytopic membrane proteins: membrane segments assemble within translocation channels prior to membrane integration. Cell 85, 379-389.
6. Chattopadhyay, S.K., Das, P.K., and Hug, G.L. (1983). Photoprocesses in diphenylpolyenes. 2. excited-state interactions with stable free radicals. J. Am. Chem. Soc. 105, 6205-6210.
7. Crowley, K.S., Reinhart, G.D., and Johnson, A.E. (1993). The signal sequence moves through a ribosomal tunnel into a noncytoplasmic aqueous environment at the ER membrane early in translocation. Cell 73, 1101-1115.
8. Crowley, K.S., Liao, S., Worrell, V.E., Reinhart, G.D., and Johnson, A.E. (1994). Secretory proteins move through the endoplasmic reticulum membrane via an aqueous, gated pore. Cell 78, 461-471.
9. Davis, R.A., Thrift, R.N., Wu, G.G., and Howell, K.E. (1990). Apolipo-protein B is both integrated into and translocated across the endoplasmic reticulum membrane. Evidence for two functionally distinct pools. J. Biol. Chem. 265, 10005-10011.
10. Do, H., Falcone, D., Lin, J., Andrews, D.W., and Johnson, A.E. (1996). The cotranslational integration of membrane proteins into the phospholipid bilayer is a multistep process. Cell 85, 369-378.
11. Eftink, M.R., and Ghiron, C.A. (1981). Fluorescence quenching studies with proteins. Anal. Biochem. 114, 199-227.
12. Hanein, D., Matlack, K.E., Jungnickel, B., Plath, K., Kalies, K.-U., Miller, K.R., Rapoport, T.A., and Akey, C.W. (1996). Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation. Cell 87, 721-732.
13. Harlow, E., and Lane, D. (1988). Antibodies: A Lab Manual (Cold Spring Harbor, New York: Cold Spring Harbor Press).
14. Hedge, R.S., and Lingappa, V.R. (1996). Sequence-specific alteration of the ribosome-membrane junction exposes nascent secretory proteins to the cytosol. Cell 85, 217-228.
15. Herron, J.N., He, X., Mason, M.L., Voss, E.W., Jr., and Edmundson, A.B. (1989). Three-dimensional structure of a fluorescein-Fab complex crystallized in 2-methyl-2,4-pentanediol. Proteins: Struct. Funct. Genet. 5, 271-280.
16. High, S., Martoglio, B., Görlich, D., Andersen, S.S.L., Ashford, A.J., Giner, A., Hartmann, E., Prehn, S., Rapoport, T.A., Dobberstein, B., and Brunner, J. (1993). Site-specific photocross-linking reveals that Sec61 p and TRAM contact different regions of a membrane-inserted signal sequence. J. Biol. Chem. 268, 26745-26751.
17. Johnson, A.E., Liao, S., Lin, J., Hamman, B., Do, H., Cowie, A., and Andrews, D.W. (1995). The environment of nascent secretory and membrane proteins at the endoplasmic reticulum membrane during translocation and integration. Cold Spring Harbor Symp. Quant. Biol. 60, 71-82.
18. Krieg, U.C., Johnson, A.E., and Walter, P. (1989). Protein translocation across the endoplasmic reticulum membrane: identification by photocrosslinking of a 39 kDa integral membrane glycoprotein as part of a putative translocation tunnel. J. Cell Biol. 709, 2033-2043.
19. Lakowicz, J.R. (1983). Principles of Fluorescence Spectroscopy (New York: Plenum Press).
20. Li, M., Dyda, F., Benhar, I., Pastan, I., and Davies, D.R. (1996). Crystal structure of the catalytic domain of Pseudomonas exotoxin A complexed with a nicotinamide adenine dinucleotide analog: implications for the activation process and for ADP ribosylation. Proc. Natl. Acad. Sci. USA 93, 6902-6906.
21. Malkin, L.I., and Rich, A. (1967). Partial resistance of nascent polypeptide chains to proteolytic digestion due to ribosomal shielding. J. Mol. Biol. 26, 329-346.
22. McCracken, A.A., and Brodsky, J.L. (1996). Assembly of ER-associated protein degradation in vitro: dependence on cytosol, calnexin, and ATP. J. Cell Biol. 132, 291-298.
23. Mothes, W., Prehn, S., and Rapoport, T.A. (1994). Systematic probing of the environment of a translocating secretory protein during translocation through the ER membrane. EMBO J. 13, 3937-3982.
24. Nicchitta, C.V., and Blobel, G. (1993). Lumenal proteins of the mammalian endoplasmic reticulum are required to complete protein translocation. Cell 73, 989-998.
25. Panzner, S., Dreier, L., Hartmann, E., Kostka, S., and Rapoport, T.A. (1995). Posttranslational protein transport in yeast reconstituted with a purified complex of Sec proteins and Kar2p. Cell 81, 561-570.
26. Rapoport, T.A., Jungnickel, B.,and Kutay, U. (1996). Protein translocation across the eukaryotic endoplasmic reticulum and bacterial inner membranes. Annu. Rev. Biochem. 65, 271-303.
27. Shaw, A.S., Rottier, P.J., and Rose, J.K. (1988). Evidence for the loop model of signal-sequence insertion into the endoplasmic reticulum. Proc. Natl. Acad. Sci. USA 85, 7592-7596.
28. Simon, S.M., and Blobel, G. (1991). A protein-conducting channel in the endoplasmic reticulum. Cell 65, 371-380.
29. Walter, P., and Johnson, A.E. (1994). Signal sequence recognition and protein targeting to the endoplasmic reticulum membrane. Annu. Rev. Cell Biol. 10, 87-119.
30. Walter, P., and Lingappa, V.R. (1986). Mechanisms of protein translocation across the endoplasmic reticulum membrane. Annu. Rev. Cell Biol. 2, 499-516.
31. Wiertz, E.J.H.J., Jones, T.R., Sun, L., Bogyo, M., Geuze, H.J., and Ploegh, H.L. (1996a). The human cytomegalovirus US11 gene product dislocates MHC class I heavy chains from the endoplasmic reticulum to the cytosol. Cell 84, 669-779.
32. Wiertz, E.J.H.J.,Tortorella, D., Bogyo, M., Yu, J., Mothes, W., Jones, T.R., Rapoport, T.A., and Ploegh, H.L. (1996b). Sec61-mediated transfer of a membrane protein from the endoplasmic reticulum to the proteasome for destruction. Nature 384, 432-438.
33. Wilkinson, B.M.,Critchley, A.J., and Stirling, C.J. (1996). Determination of the transmembrane topology of yeast Sec61p, an essential component of the endoplasmic reticulum translocation complex. J. Biol. Chem. 277, 25590-25597.
34. Wilson, I.A., Rini, J.M., Fremont, D.H., Fieser, G.G., and Stura, E.A. (1991). X-ray crystallographic analysis of free and antigen-complexed Fab fragments to investigate structural basis of immune recognition. Meth. Enzymol. 203, 153-176.
35. Wolin, S.L., and Walter, P. (1988). Ribosome pausing and stacking during translation of a eukaryotic mRNA. EMBO J. 7, 3559-3569.
36. Zens, A.P., Williams, T.J., Wisowaty, J.C., Fisher, R.R., Dunlap, R.B., Bryson, T.A., and Ellis, P.D. (1975). Nuclear magnetic studies on pyridine dinucleotides. II. Solution conformational dynamics of nicotinamide adenine dinucleotide and nicotinamide adenine mononucleotide as viewed by proton T1 measurements. J. Am. Chem. Soc. 97, 2850-2857.
37. Zens, A.P., Bryson, T.A., Dunlap, R.B., Fisher, R.R., and Ellis, P.D. (1976). Nuclear magnetic resonance studies on pyridine dinucleotides. 7. The solution conformational dynamics of the adenosine portion of nicotinamide adenine dinucleotide and other related purine containing compounds. J. Am. Chem. Soc. 98, 7559-7564.