Role of the cytoplasmic segments of Sec61α in the ribosome-binding and translocation-promoting activities of the Sec61 complex

Journal of Cell Biology

Volumn 150, Issue 1, 2000, Pages 53-64

  Raden, David ^a   Song, Weiqun ^a   Gilmore, Reid ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

Endoplasmic reticulum; Protein targeting; Protein topology; Protein translocation; Translocon structure

Indexed keywords

MEMBRANE PROTEIN;

ARTICLE; CYTOSOL; ENDOPLASMIC RETICULUM; MICROSOME; NONHUMAN; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN LOCALIZATION; PROTEIN TRANSPORT; RIBOSOME SUBUNIT;

ANIMALS; BINDING SITES; BIOLOGICAL TRANSPORT; CYTOPLASM; DOGS; MACROMOLECULAR SUBSTANCES; MEMBRANE PROTEINS; MICROSOMES; PANCREAS; PEPTIDE CHAIN INITIATION, TRANSLATIONAL; PEPTIDE HYDROLASES; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RIBOSOMES; SEEDS; SIGNAL RECOGNITION PARTICLE; THERMOLYSIN; TRITICUM;

EID: 0034631835     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.150.1.53     Document Type: Article

References (44)

1. Adelman, MR., D.D. Sabatini, and G. Blobel. 1973. Ribosome-membrane interaction. Nondestructive disassembly of rat liver rough microsomes into ribosomal and membrane components. J. Cell Biol. 56:206-229.
2. Akiyama, Y., and K. Ito. 1987. Topology analysis of the SecY protein, an integral membrane protein involved in protein export in Escherichia coli. EMBO (Eur. Mol. Biol. Organ.) J. 6:3465-3470.
3. Beckmann, R., D. Bubeck, R. Grassucci, F. Penczek, A. Verschoor, G. Blobel, and J. Frank. 1997. Alignment of conduits for the nascent polypeptide chain in the ribosome-Sec61 complex. Science. 278:2123-2126.
4. Borgese, N., W. Mok, G. Kreibich, and D.D. Sabatini. 1974. Ribosomal-membrane interaction: in vitro binding of ribosomes to microsomal membranes. J. Mol. Biol. 88:559-580.
5. Collins, P., and R. Gilmore. 1991. Ribosome binding to the endoplasmic reticulum: u 180-kD protein identified by cross-linking to membrane bound ribosomes is not required for ribosome binding activity. J. Cell Biol. 114:639-649.
6. Connolly, T., and R. Gilmore. 1989. The signal recognition particle receptor mediates the GTP-dependent displacement of SRP from the signal sequence of the nascent polypeptide. Cell. 57:599-610.
7. Connolly, T., P. Collins, and R. Gilmore. 1989. Access of protcinase K to partially translocated nascent polypeptides in intact and detergent-solubilized membranes. J. Cell Biol. 108:299-307.
8. Copeland, C.S., R.W. Doms, E.M. Bolzau, R.G. Webster, and A. Helenius. 1986. Assembly of influenza hemagglutinin trimers and its role in intracellular transport. J. Cell Biol. 103:1179-1191.
9. Crowley, K.S., S. Liao, V.E. Worrell, G.D. Reinhart, and A.E. Johnson. 1994. Secretory proteins move through the endoplasmic reticulum via an aqueous, gated pore. Cell. 78:461-471.
10. Crowley, K.S., G.D. Reinhart, and A.E. Johnson. 1993. The signal sequence moves through a ribosomal tunnel into a noncytoplasmic aqueous environment at the ER membrane early in translocation. Cell. 73:1101-1115.
11. Doms, R.W., and A. Helenius. 1986. Quaternary structure of influenza virus hemagglutinin after acid treatment. J. Virol. 60:833-839.
12. Gilmore, R., and G. Blobel. 1985. Translocation of secretory proteins across the microsomal membrane occurs through an environment accessible to aqueous perturbants. Cell. 42:497-505.
13. Gilmore, R., P. Collins, J. Johnson, K. Kellaris, and P. Rapiejko. 1991. Transcription of full length and truncated mRNA transcripts to study protein translocation across the endoplasmic reticulum. Methods Cell Biol. 34:223-239.
14. Görlich, D., and T.A. Rapoport. 1993. Protein translocation into proteoliposomes reconstituted from purified components of the ER membrane. Cell. 75:615-630.
15. Görlich, D., S. Prehn, E. Hartmann, K.-U. Kalies, and T.A. Rapoport. 1992. A mammalian homologue of Sec61p and SecYp is associated with ribosomes and nascent polypeptides during translocation. Cell. 71:489-503.
16. Hamman, B.D., J.-C. Chen, E.E. Johnson, and A.E. Johnson, 1997. The aqueous pore through the translocon has a diameter of 40-60 Å during cotranslational protein translocation at the ER membrane. Cell. 89:535-544.
17. Hamman, B.D., L.M. Hendershot, and A.E. Johnson. 1998. BiP maintains the permeability barrier of the ER membrane by sealing the lumenal end of the translocation pore before and early in translocation. Cell. 92:747-758.
18. Hanein, D., K.E.S. Matlack, B. Jungnickel, K. Plath, K.-U. Kalies, K.R. Miller, T.A. Rapoport, and C.W. Akey. 1996. Oligomeric rings of the Sec61p complex induced by ligands required for protein translocation. Cell. 87:721-732.
19. Hebert, D.N., and A. Carruthers. 1991. Cholate-solubilized erythrocyte glucose transporters exist as a mixture of homodimers and homotetramers. Biochemistry. 30:4654-4658.
20. Hortsch, M., D. Avossa, and D.I. Meyer. 1986. Characterization of secretory protein translocation: ribosome-membrane interaction in endoplasmic reticulum. J. Cell Biol. 103:241-253.
21. Ichimura, T., T. Ohsumi, Y. Shindo, T. Ohwada, H. Yagame, Y. Monose, S. Omata, and H. Sugano. 1992. Isolation and some properties of a 34-kD membrane protein that may be essential for ribosome binding in rat liver rough microsomes. FEBS (Fed. Eur. Biochem. Soc.) Lett. 296:7-10.
22. Jungnickel, B., and T.A. Rapoport. 1995. A posttranslational signal sequence recognition event in the endoplasmic reticulum membrane. Cell. 82:261-270.
23. Kalies, K.-U., D. Görlich, and T.A. Rapoport. 1994. Binding of ribosomes to the rough endoplasmic reticulum is mediated by the Sec61p complex. J. Cell Biol. 126:925-934.
24. Kelleher, D.J., and R. Gilmore. 1997. DAD1, the defender against apoptotic cell death, is a subunit of the mammalian oligosaccharyltransferase. Proc. Natl. Acad. Sci. USA. 94:4994-4999.
25. Lauring, B., G. Kreibich, and M. Wiedmann. 1995a. The intrinsic ability of ribosomes to bind to endoplasmic reticulum membranes is regulated by signal recognition particle and nascent-polypeptide-associated complex. Proc. Natl. Acad. Sci. USA. 92:9435-9439.
26. Lauring, B., H. Sakai, G. Kreibich, and M. Wiedmann. 1995b. Nascent polypeptide-associated complex protein prevents mistargeting of nascent chains to the endoplasmic reticulum. Proc. Natl. Acad. Sci. USA. 92:5411-5415.
27. Mothes, W., S. Prehn, and T.A. Rapoport. 1994. Systematic probing of the environment of a translocating secretory protein during translocation through the ER membrane. EMBO (Eur. Mol. Biol. Organ.) J. 13:3973-3982.
28. Neuhof, A., M.M. Rolls, B. Jungnickel, K.-U. Kalies, and T.A. Rapoport. 1998. Binding of signal recognition particle gives ribosome/nascent chain complexes a competitive advantage in endoplasmic reticulum membrane interaction. Mol. Biol. Cell. 9:103-115.
29. Nicchitta, C.V., and G. Blobel. 1989. Nascent secretory chain binding and translocation are distinct processes: differentiation by chemical alkylation. J. Cell Biol. 108:789-795.
30. Nicchitta, C.V., and T. Zheng. 1997. Regulation of ribosome-membrane junction at early stages of presecretory protein translocation across the endoplasmic reticulum. J. Cell Biol. 139:1697-1708.
31. Plath, K., W. Mothes, B.M. Wilkinson, C.J. Stirling, and T.A. Rapoport. 1998. Signal sequence recognition in posttranslational protein transport across the yeast ER membrane. Cell. 94:795-807.
32. Raden, D., and R. Gilmore. 1998. Signal recognition particle-dependent targeting of ribosomes to the rough endoplasmic reticulum in the absence and presence of the nascent polypeptide associated complex. Mol. Biol. Cell. 9:117-130.
33. Rapiejko, P.J., and R. Gilmore. 1994. Signal sequence recognition and targeting of ribosomes to the endoplasmic reticulum by the signal recognition particle do not require GTP. Mol. Biol. Cell. 5:887-897.
34. Rapiejko, P.J., and R. Gilmore. 1997. Empty site forms of the SRP54 and SRα GTPases mediate targeting of ribosome-nascent chain complexes to the endoplasmic reticulum. Cell. 89:703-713.
35. Savitz, A.J., and D.I. Meyer. 1990. Identification of a ribosome receptor in the rough endoplasmic reticulum. Nature. 346:540-544.
36. Savitz, A.J., and D.I. Meyer. 1993. 180-kD ribosome receptor is essential for both ribosome binding and protein translocation. J. Cell Biol. 120:853-863.
37. Schägger, H., and G. von Jagow. 1987. Tricine-sodium dodecyl-sulphate polyacrylamide gel electrophoresis for the separation of proteins in the range from 1-100 kDa. Anal. Biochem. 166:368-379.
38. Simon, S.M., and G. Blobel. 1991. A protein-conducting channel in the endoplasmic reticulum. Cell. 65:371-380.
39. Song, W., D. Raden, E. Mandon, and R. Gilmore. 2000. Role of Sec61α in the regulated transfer of the ribosome-nascent chain complex from the signal recognition particle to the translocation channel. Cell. 100:333-343.
40. Voigt, S., B. Jungnickel, E. Hartmann, and T.A. Rapoport. 1996. Signal sequence-dependent function of the TRAM protein during early phases of protein transport across the endoplasmic reticulum. J. Cell Biol. 134:25-35.
41. Walter, P., and A.E. Johnson. 1994. Signal sequence recognition and protein targeting to the endoplasmic reticulum membrane. Annu. Rev. Cell Biol. 10: 87-119.
42. Waller, P., I. Ibrahimi, and G. Blobel. 1981. Translocation of proteins across the endoplasmic reticulum. I. Signal recognition protein (SRP) binds to in-vitro-assembled polysomes synthesizing secretory protein. J. Cell Biol. 91:545-550.
43. Wilkinson, B.M., A.J. Critchley, and C.J. Stirling. 1996. Determination of the transmembrane topology of yeast Sec61p; an essential component of the ER translocation complex. J. Biol. Chem. 271:25590-25597.
44. Wilkinson, B.M., Y. Esnault, R.A. Craven, F. Skiba, J. Fieschi, F. Képès, and C.J. Stirling. 1997. Molecular architecture of the ER translocase probed by chemical crosslinking of Ssslp to complementary fragments of Sec61p. EMBO (Eur. Mol. Biol. Organ.) J. 16:4549-4559.