Error-prone PCR of Vitreoscilla hemoglobin (VHb) to support the growth of microaerobic Escherichia coli

Biotechnology and Bioengineering

Volumn 70, Issue 4, 2000, Pages 446-455

  Andersson, Charlotte I J ^a   Holmberg, Niklas ^b   Farrés, Judith ^b   Bailey, James E ^b   Blow, Leif ^a   Kallio, Pauli T ^b  

^a LUND UNIVERSITY   (Sweden)

^b ETH ZURICH   (Switzerland)

Author keywords

Error prone PCR; Escherichia coli; Microaerobic growth; VHb mutants; Vitreoscilla hemoglobin (VHb)

Indexed keywords

BIOREACTORS; CELL CULTURE; CLONING; ESCHERICHIA COLI; GROWTH KINETICS; MUTAGENESIS; PROTEINS;

GENE ENCODING; MICROAEROBIC GROWTH; POLYMERASE CHAIN REACTION; VITREOSCILLA HEMOGLOBIN (VHB);

HEMOGLOBIN;

ACETIC ACID; HEMOGLOBIN; MUTANT PROTEIN;

AEROBIC BACTERIUM; AMINO ACID SUBSTITUTION; AMINO TERMINAL SEQUENCE; ARTICLE; BACTERIAL GROWTH; BIOREACTOR; CONTROLLED STUDY; CULTURE MEDIUM; ESCHERICHIA COLI; GENE EXPRESSION; GENE MUTATION; GRAM NEGATIVE BACTERIUM; NONHUMAN; POLYMERASE CHAIN REACTION; VITREOSCILLA;

ACETATES; AEROBIOSIS; BACTERIAL PROTEINS; BETA-LACTAMASES; BIOREACTORS; BIOTECHNOLOGY; CARBON MONOXIDE; CELL DIVISION; ESCHERICHIA COLI; ETHANOL; GENETIC ENGINEERING; HEMOGLOBINS; MUTATION; POLYMERASE CHAIN REACTION; RECOMBINANT PROTEINS;

BACTERIA (MICROORGANISMS); ESCHERICHIA COLI; NEGIBACTERIA; VITREOSCILLA;

EID: 0034694155     PISSN: 00063592     EISSN: None     Source Type: Journal    
DOI: 10.1002/1097-0290(20001120)70:4<446::AID-BIT10>3.0.CO;2-K     Document Type: Article

References (48)

1. Inverse metabolic engineering: A strategy for directed genetic engineering of useful phenotypes
2. Determinants of a protein fold. Unique features of the globin amino acid sequences
3. The metabolic effects of native and transgenic hemoglobins on plants
4. Randomization of genes by PCR mutagenesis
5. Tuning the activity of an enzyme for unusual environments: Sequential random mutagenesis of subtilisin E for catalysis in dimethylformamide
6. 31P NMR and saturation transfer study
7. Asn249Tyr substitution at the coenzyme binding domain activates Sulfolobus solfataricus alcohol dehydrogenase and increases its thermal stability
8. Purification and aqueous 2-phase partitioning properties of recombinant Vitreoscilla hemoglobin
9. Directed evolution of an esterase from Pseudomonas fluorescens. Random mutagenesis by error-prone PCR or a mutator strain and identification of mutants showing enhanced enantioselectivity by a resorufin-based fluorescence assay
10. Transgenic tobacco expressing Vitreoscilla hemoglobin exhibits enhanced growth and altered secondary metabolite production
11. Intracellular expression of Vitreoscilla hemoglobin (VHb) enhances total protein secretion and improves the production of α-amylase and neutral protease in Bacillus subtilis
12. Intracellular expression of Vitreoscilla hemoglobin alters Escherichia coli energy metabolism under oxygen-limited conditions
13. Expression of Vitreoscilla hemoglobin is superior to horse heart myoglobin and yeast flavohemoglobin expression for enhancing Escherichia coli growth in a microaerobic bioreactor
14. Heterologous expression of a bacterial haemoglobin improves the growth properties of recombinant Escherichia coli
15. The Vitreoscilla hemoglobin gene: Molecular cloning, nucleotide sequence and genetic expression in Escherichia coli
16. Characterization of the oxygen-dependent promoter of the Vitreoscilla hemoglobin gene in Escherichia coli
17. Evidence for partial export of Vitreoscilla hemoglobin into the periplasmic space in Escherichia coli: Implications for protein function
18. Expression of intracellular hemoglobin improves protein synthesis in oxygen-limited Escherichia coli
19. Presence of the bacterial hemoglobin gene improves α-amylase production of a recombinant Escherichia coli strain
20. Terminal oxidases of Escherichia coli: Aerobic respiratory chain
21. Effects of oxygen on recombinant protein production
22. Cleavage of structural proteins during the assembly of the head of bacteriophage T4
23. A method for random mutagenesis of a defined DNA segment using a modified polymerase chain reaction
24. Evolutionary molecular engineering by random elongation mutagenesis
25. Improved erythromycin production in a genetically engineered strain of Saccharopolyspora erythraea
26. Directed evolution of para-nitrobenzyl esterase for aqueous-organic solvents
27. Development of the multiple sequence approximation within the Agadir model of α-helix formation. Comparison with Zimm-Bragg and Lifson-Roig formalisms
28. Expression of Vitreoscilla hemoglobin in Escherichia coli enhances ribosome and tRNA levels: A flow field-flow fractionation study
29. A novel method for detection β-1actamase by using a chromogenic cephalosporin substrate
30. Photodissociation of oxygenated cytochrome o(s) (Vitreoscilla) and kinetic studies of reassociation
31. Regulation of oxygen affinity of hemoglobin: Influence of structure of the globin on the heme iron
32. Combinatorial cassette mutagenesis as a probe of the informational content of protein sequences
33. Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase
34. DNA sequencing with chain-termination inhibitors
35. Random mutagenesis on the Pseudomonas lipase activator protein, lipB: Exploring amino acid residues required for its function
36. The transition state transcription regulator abrB of Bacillus subtilis is a DNA binding protein
37. Expression, purification, crystallization, and preliminary X-ray diffraction analysis of the homodimeric bacterial hemoglobin from Vitreoscilla stercoraria
38. Unusual structure of the oxygen-binding site in the dimeric bacterial hemoglobin from Vitreoscilla sp
39. Improvement of Escherichia coli microaerobic oxygen metabolism by Vitreoscilla hemoglobin: New insights from NAD(P)H fluorescence and culture redox potential
40. Effect of Vitreoscilla hemoglobin dosage on microaerobic Escherichia coli carbon and energy metabolism
41. Intracellular expression of Vitreoscilla hemoglobin modifies microaerobic Escherichia coli metabolism through elevated concentration and specific activity of cytochrome o
42. Primary sequence of a dimeric bacterial haemoglobin from Vitreoscilla
43. Structure and function of bacterial hemoglobin and related proteins
44. Western blotting
45. Mechanisms of cytoplasmic hemoglobin and myoglobin function
46. Improved M13 phage cloning vectors and host strains: Nucleotide sequences of the M13mp18 and pUC19 vectors
47. Directed evolution of subtilisin E in Bacillus subtilis to enhance total aqueous dimethylformamide