Perspectives in the biological function and the technological application of polygalacturonases

Applied Microbiology and Biotechnology

Volumn 53, Issue 4, 2000, Pages 366-375

  Lang, C ^a   Dornenburg H ^a  

^a TECHNISCHE UNIVERSITÄT BERLIN   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

POLYGALACTURONASE;

ENZYME SYNTHESIS; FOOD PROCESSING; HOST PARASITE INTERACTION; NONHUMAN; PLANT GROWTH; SHORT SURVEY; TRANSGENIC PLANT;

FUNGI;

EID: 0034011112     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s002530051628     Document Type: Short Survey

References (116)

1. Allen RL, Lonsdale DM (1992) Sequence analysis of three members of the maize polygalacturonase gene family expressed during pollen development. Plant Mol Biol 20: 343-345
2. Andrews PK, Shulin L (1995) Cell wall hydrolytic enzyme activity during development of nonclimacteric sweet cheery (Prunus avium L.) fruit. J Hortic Sci 70: 561-567
3. Annis SL, Goodwin PH (1996) Comparison of cell-wall degrading enzymes produced by highly and weakly virulent isolates of Leptosphaeria maculans in culture. Microbiol Res 151: 401-406
4. Annis SL, Goodwin PH (1997) Inhibition of polygalacturonase activity produced by Leptosphaeria maculans with stem extracts of canola, and the relationship of inhibition to resistance. J Phytopathol 145: 217-223
5. Atkinson RG, Gardner RC (1993) A polygalacturonase gene from kiwifruit (Actinidia deliciosa). Plant Physiol 103: 669-670
6. Atkinson RG, Bolitho KM, Wright MA, Iturriagagoita-Bueno T, Reid SJ, Ross GS (1998) Apple ACC-oxidase and polygalacturonase: ripening-specific gene expression and promoter analysis in transgenic tomato. Plant Mol Biol 38: 449-460
7. Baayen RP, Schoffelmeer EAM, Toet S, Elgersma DM (1997) Fungal polygalacturonase activity reflects susceptibility of carnation cultivars to fusarium wilt. Eur J Plant Pathol 103: 15-23
8. Barnby FM, Morpeth FF, Pyle DL (1990) Endopolygalacturonase production from Kluyveromyces marxianus. I. Resolution, purification and partial characterization of the enzyme. Enzyme Microbiol Technol 12: 891-897
9. Ben-Arie R, Saks Y, Sonego L, Frank A (1996) Cell wall metabolism in gibberellin-treated persimmon fruits. Plant Growth Regul 19: 25-33
10. Bennett A, Labavitch JM, Powell A, Stotz H (1996) Plant inhibitors of fungal polygalacturonases and their use to control fungal disease. US Patent 5,569,830, Oct 29, 1996
11. Bergey DR, Orozco-Cardenas M, de Moura DS, Ryan CA (1999) A wound-and systemin-inducible polygalacturonase in tomato leaves. Proc Natl Acad Sci USA 96: 1756-1760
12. Bergmann CW, Ito Y, Singer D, Albersheim P, Darvill AG, Benhamou N, Nuss L, Salvi G, Cervone F, De Lorenzo G (1994) Polygalacturonase-inhibiting protein accumulates in Phaseolus vulgaris L. in response to wounding, elicitors and fungal infection. Plant J 5: 625-634
13. Biely P, Benen J, Heinrichova K, Kester HC, Visser J (1996) Inversion of configuration during hydrolysis of alpha-1,4-galacturonidic linkage by three Aspergillus polygalacturonases. FEBS Lett 382: 249-255
14. Biggs AR, El Kholi MM, El Neshawy S, Nickerson R (1997) Effects of calcium salts on growth, polygalacturonase activity, and infection of peach fruit by Monilinia fructicola. Plant Dis 81: 399-403
15. Blanco P, Sieiro C, Reboredo NM, Villa TG (1997) Genetic determination of polygalacturonase production in wild-type and laboratory strains of Saccharomyces cerevisiae. Arch Microbiol 167: 284-288
16. Boudart G, Dechamp-Guillaume G, Lafitte C, Ricart G, Barthe JP, Mazau D, Esquerre-Tugaye MT (1995) Elicitors and suppressors of hydroxyproline-rich glycoprotein accumulation are solubilized from plant cell walls by endopolygalacturonase. Eur J Biochem 232: 449-457
17. Buescher RW, Hudson JM, Adams JR (1981) Utilization of calcium to reduce pectinolytic softening of cucumber pickles in low salt conditions. Lebensm Wiss Technol 14: 65-69
18. Bussink HJ, Buxton FP, Visser J (1991) Expression and sequence comparison of the Aspergillus niger and Aspergillus tubigensis genes encoding polygalacturonase II. Curr Genet 19: 467-474
19. Bussink HJ, Buxton FP, Fraaye BA, de Graaff LH, Visser J (1992) The polygalacturonases of Aspergillus niger are encoded by a family of diverged genes. Eur J Biochem 208: 83-90
20. Caprari C, Richter A, Bergmann C, Lo Cicero S, Salvi G, Cervone F, De Lorenzo G (1993) Cloning and characterization of a gene encoding the endogalacturonase of Fusarium moniliforme. Mycol Res 97: 497-505
21. Caprari C, Mattei B, Basile ML, Salvi G, Crescenzi V, De Lorenzo G, Cervone F (1996) Mutagenesis of endopolygalacturonase from Fusarium moniliforme: histidine residue 234 is critical for enzymatic and macerating activities and not for binding to polygalacturonase-inhibiting protein (PGIP). Mol Plant Microbe Interact 9: 617-624
22. Cary JW, Brown R, Cleveland TE, Whitehead M, Dean RA (1995) Cloning and characterization of a novel polygalacturonase-encoding gene from Aspergillus parasiticus. Gene 153: 129-133
23. Centis S, Guillas I, Sejalon N, Esuerre-Tugaye MT (1997) Endopolygalacturonase genes from Colletotrichum lindemuthianum: cloning of CLPG2 and comparison of its expression to that of CLPG1 during saprophytic and parasitic growth of the fungus. Mol Plant Microbe Interact 10: 769-775
24. Cerda JJ (1987) The role of grapefruit pectin in health and disease. Trans Am Clin Climatol Assoc 99: 203-213
25. Chun JP, Huber DJ (1998) Polygalacturonase-mediated solubilization and depolymerization of pectic polymers in tomato fruit cell walls. Regulation by pH and ionic conditions. Plant Physiol 117: 1293-1299
26. Cleveland TE, Cotty PJ (1991) Invasiveness of Aspergillus flavus isolates in unwounded cotton balls is associated with production of specific fungal polygalacturonase. Phytopathology 81: 155-158
27. Crittenden RG, Plaine MJ (1996) Production, properties and applications of food-grade oligosaccharides. Trends Food Sci Technol 7: 353-361
28. Crotti LB, Terenzi HF, Jorge JA, de Lourdes M, Polizeli ML (1998) Regulation of pectic enzymes from the exo-1 mutant strain of Neurospora crassa: effects of glucose, galactose, and galacturonic acid. J Basic Microbiol 38: 181-188
29. De Bary A (1886) Ueber einige Sclerotinien und Sclerotien Krankheiten. Bot Ztg 44: 376-480
30. Dean RA, Timberlake WE (1989) Production of cell-wall degrading enzymes by Aspergillus nidulans: A model system for fungal pathogenesis of plants. Plant Cell 1: 265-273
31. Devi NA, Rao AG (1998) Conformation of polygalacturonase-II from Aspergillus carbonarius - a spectroscopic study. Biochem Mol Biol Int 44: 79-87
32. Devoto A, Leckie F, Lupotto E, Cervone F, De Lorenzo G (1998) The promoter of a gene encoding a polygalacturonase-inhibiting protein of Phaseolus vulgaris L. is activated by wounding but not by elicitors or pathogen infection. Planta 205: 165-174
33. Di Pietro A, Roncero MI (1996) Purification and characterization of an exo-polygalacturonase from the tomato vascular wilt pathogen Fusarium oxysporum f.sp. lycopersici. FEMS Microbiol Lett 145: 295-299
34. Di Pietro A, Roncero MI (1998) Cloning, expression, and role in pathogenicity of pg1 encoding the major extracellular endopolygalacturonase of the vascular wilt pathogen Fusarium oxysporum. Mol Plant Microbe Interact 11: 91-98
35. Downs CG, Brady CJ, Gooley A (1992) Exopolygalacturonase protein accumulates late in peach fruit ripening. Physiol Plant 85: 133-140
36. Dumas B, Centis S, Sarrazin N, Esquerre-Tugaye E (1999) Use of green fluorescent protein to detect expression of an endopolygalacturonase gene of Colletotrichum lindemuthianum during bean infection. Appl Environ Microbiol 65: 1769-1771
37. Ebbelaar MEM, Tucker GA, Laats MM, van Dijk C, Stolle-Smits T, Recourt K (1996) Characterization of pectinases and pectin methylesterase cDNAs in pods of green beans (Phaseolus vulgaris L.). Plant Molec Biol 31: 1141-1151
38. Endreß HU (1996) Pektin und Pektinenzyme in der Technologie pflanzlicher Lebensmittel. Flüssiges Obst 63: 567-574
39. Errington N, Tucker GA, Mitchell JR (1998) Effect of genetic down-regulation of polygalacturonase and pectin esterase activity on rheology and composition of tomato juice. J Sci Food Agric 76: 515-519
40. Fraissinet-Tachet L, Fevre M (1996) Regulation by galacturonic acid of pectinolytic enzyme production by Sclerotinia sclerotiorum. Curr Microbiol 33: 49-53
41. Fraissinet-Tachet L, Reymond-Cotton P, Fevre M (1995) Characterization of a multigene family encoding an endogalacturonase in Sclerotinia sclerotiorum. Curr Genet 29: 96-99
42. Galiotou-Panayotou M, Kalantzi O, Aggelis G (1998) Modelling of simultaneous production of polygalacturonase and exopolysaccharide by Aureobasidium pullulans ATHUM 2915. Antonie Van Leeuwenhoek J Microbiol Serol 73: 155-162
43. Gao S, Choi GH, Shain L, Nuss DL (1996) Cloning and targeted disruption of enpg-1, encoding the major in vitro extracellular endopolygalacturonase of the chestnut blight fungus, Cryphonectria parasitica. Appl Environ Microbiol 62: 1984-1990
44. García-Romera I, Fry SC (1997) Upper limits for endogenous oligogalacturonides and free galacturonic acids in rose cell-suspension cultures: Implications for the action of exo-and endo-polygalacturonases in vivo. J Plant Physiol 150: 241-246
45. Glinka EM, Protsenko MA (1998) Polygalacturonase inhibiting protein in plant cell walls. Biochemistry (Mosc) 63: 1015-1020
46. Gognies S, Gainvors A, Aigle M, Belarbi A (1999) Cloning, sequence analysis and overexpression of a Saccharomyces cerevisiae endopolygalacturonase-encoding gene. Yeast 15: 11-22
47. Hadfield KA, Bennett AB (1998) Polygalacturonases: Many genes in search of a function. Plant Physiol 117: 337-343
48. Hadfield KA, Rose JK, Yaver DS, Berka RM, Bennett AB (1998) Polygalacturonase gene expression in ripe melon fruit supports a role for polygalacturonase in ripening-associated pectin disassembly. Plant Physiol 117: 363-373
49. Hassan AK, Schulz C, Sacristan MD, Wöstemeyer J (1991) Biochemical and molecular tools for the differentiation of aggressive and non-aggressive isolates of the oilseed rape pathogen, Phoma lingam. J Phytopathol 131: 120-136
50. Iwasaki Ki, Inoue M, Matsubara Y (1998) Continuous hydrolysis of pectate by immobilized endo-polygalacturonase in a continuously stirred tank reactor. Biosci Biotech Biochem 62: 262-267
51. John ME, Petersen MW (1994) Cotton (Gossipium hirsutum L.) pollen-specific polygalacturonase mRNA: tissue and temporal specificity of its promoter in transgenic tobacco. Plant Mol Biol 26: 1989-1993
52. Jones CS, Davies HV, McNicol RJ, Taylor MA (1998) Cloning of three genes up-regulated in ripening raspberry fruit (Rubus ideaus cv. Glen Clova). J Plant Physiol 153: 643-648
53. Jouany JP, Mathieu F, Senaud J, Bohatier J, Bertin G, Mercier M (1998) The effect of Saccharomyces cerevisiae and Aspergillus oryzae on the digestion of the cell wall fraction of a mixed diet in defaunated and refaunated sheep rumen. Reprod Nutr Dev 38: 401-416
54. Karam NE, Belarbi A (1995) Detection of polygalacturonases and pectin esterases in lactic acid bacteria. World J Microbiol Biotechnol 11: 559-563
55. Kester HC, Kusters-van Someren MA, Muller Y, Visser J (1996) Primary structure and characterization of an exopolygalacturonase from Aspergillus tubingensis. Eur J Biochem 240: 738-746
56. Ketsa S, Chidtragool S, Klein JD, Lurie S (1998) Effect of heat treatment on changes in softening, pectic substances and activities of polygalacturonase, pectinesterase and β-galactosidase of ripening mango. J Plant Physiol 153: 457-461
57. Kitamoto N, Kimura T, Kito Y, Ohmiya K, Tsukagoshi N (1993) Structural features of a polygalacturonase gene cloned from Aspergillus oryzae KBN616. FEMS Microbiol Lett 111: 37-41
58. Kitamoto N, Matsui J, Kawai Y, Kato A, Yoshino S, Ohmiya K, Tsukagoshi N (1998) Utilization of the TEF1-alpha gene (TEF1) promoter for expression of polygalacturonase genes, pgaA and pgaB, in Aspergillus oryzae. Appl Microbiol Biotechnol 50: 85-92
59. Konno H, Yamasaki Y, Katoh K (1989) Extracellular exo-polygalacturonase secreted from carrot cell cultures. Its purification and involvement in pectic polymer degradation. Physiol Plant 76: 514-520
60. Kutsunai SY, An CL, Persival FW, Laties GG, Christoffersen RE (1993) Ripening-related polygalacturonase cDNA from avocado. Plant Physiol 103: 289-290
61. Lang C, Looman AC (1995) Efficient expression and secretion of Aspergillus niger RH5344 polygalacturonase in Saccharomyces cerevisiae. Appl Microbiol Biotechnol 44: 147-156
62. Lazan H, Selamat KM, Ali ZM (1995) β-Galactosidase, polygalacturonase and pectinesterase in differential softening and cell wall modification during papaya fruit ripening. Physiol Plant 95: 106-112
63. Lester DR, Sherman WB, Atwell BJ (1996) Endopolygalacturonase and the melting flesh (M) locus in peach. J Am Soc Hortic Sci 121: 231-234
64. Lopez P, Sanchez AC, Vercet A, Burgos J (1997) Thermal resistance of tomato polygalacturonase and pectinmethylesterase at physiological pH. Z Lebensm Unters Forsch 204: 146-150
65. Matsumoto T, Cyong JC, Kiyohara H, Matsui H, Abe A, Hirano M, Danbara H, Yamada H (1993) The pectic polysaccharide from Bupleurum falcatum L. enhances immune-complexes binding to peritoneal macrophages through Fc receptor expression. Int J Immunopharmacol 15: 683-693
66. Miyairi K, Senda M, Watanabe M, Hasui S, Okuno T (1997) Cloning and sequence analysis of cDNA encoding endopolygalacturonase I from Stereum purpureum. Biosci Biotechnol Biochem 61: 655-659
67. Moore T, Bennett AB (1994) Tomato fruit polygalacturonase isozyme 1. Plant Physiol 106: 1461-1469
68. Muller S, Sandal T, Kamp-Hansen P, Dalboge H (1998) Comparison of expression systems in the yeasts Saccharomyces cerevisiae, Hansenula polymorpha, Kluyveromyces lactis, Schizosaccharomyces pombe and Yarrowia lipolytica. Cloning of two novel promoters from Yarrowia lipolytica. Yeast 14: 1267-1283
69. Munoz JA, Coronado C, Perez-Hormaeche J, Kondorosi A, Ratet P, Palomares AJ (1998) MsPG3, a Medicago sativa polygalacturonase gene expressed during the alfalfa-Rhizobium meliloti interaction. Proc Natl Acad Sci USA 95: 9687-9692
70. Nagar PK (1994) Effect of some ripening retardants on fruit softening enzymes of Kinnow mandarin fruits. Ind J Plant Physiol 37: 122-124
71. Nogata Y, Ohta H, Voragen AGJ (1993) Polygalacturonase in strawberry fruit. Phytochemistry 34: 617-620
72. Olsson K (1989) Relationship between pectolytic enzyme activity and rot development in potato tubers inoculated with Fusarium solani var. coeruleum. J Phytopathol 124: 225-235
73. Parenicova L, Benen JAE, Kester HCM, Visser J (1998) pgaE encodes a fourth member of the endopolygalacturonase gene family from Aspergillus niger. Eur J Biochem 251: 72-80
74. Pathak N, Sanwal GG (1998) Multiple forms of polygalacturonase from banana fruits. Phytochemistry 48: 249-255
75. Patino B, Posada ML, Gonzales-Jaen MT, Martinez del Pozo A, Vazquez C (1997) Control of polygalacturonase synthesis in Fusarium oxysporum f.sp. radicis lycopersici. Can J Microbiol 43: 1084-1090
76. Petäistö RL, Kajander EO (1993) The ability of Gremmeniella abietina to hydrolyze polygalacturonic acid. Eur J For Pathol 23: 306-313
77. Petersen M, Sander L, Child R, van Onckelen H, Ulvskov P, Borkhardt B (1996) Isolation and characterisation of pod dehiscence zone-specific polygalacturonase from Brassica napus. Plant Mol Biol 31: 517-527
78. Polizeil M de L, Jorge JA, Terenzi HF (1991) Pectinase production by Neurospora crassa: purification and biochemical characterization of extracellular polygalacturonase activity. J Gen Microbiol 137: 1815-23
79. Pressey R (1996) Polygalacturonase inhibitors in bean pods. Phytochemistry 42: 1267-1270
80. Priya-Setu KM, Prabha TN, Tharanathan RN (1996) Post-harvest biochemical changes associated with the softening phenomen in Capsicum annuum fruits. Phytochemistry 42: 961-966
81. Ralet MC, Thibault C (1994) Extraction and characterisation of very highly methylated pectins from lemon cell walls. Carbohydr Res 260: 283-296
82. Rao MN, Kembhavi AA, Pant A (1996) Implication of tryptophan and histidine in the active site of endo-polygalacturonase from Aspergillus ustus: elucidation of the reaction mechanism. Biochim Biophys Acta 1296: 167-73
83. Rensberg P van, Zyl WH van, Pretorius (1994) Expression of the Butyrivibrio fibrisolvens endo-beta-1,4-glucanase gene together with the Erwinia pectate lyase and polygalacturonase genes in Saccharomyces cerevisiae. Curr Genet 27: 17-22
84. Reymond P, Kunz B, Paul-Pletzer K, Grimm R, Eckerskorn C, Farmer EE (1996) Cloning of a cDNA encoding a plasma membrane-associated, uronide binding phosphoprotein with physical properties similar to viral movement proteins. Plant Cell 8: 2265-2276
85. Reymond-Cotton P, Fraissinet-Tachet L, Fevre M (1996) Expression of the Sclerotinia sclerotiorum pg1 gene: possible involvement of CREA in glucose catabolite repression. Curr Genet 30: 240-245
86. Ruttkowski E, Khanh NQ, Wientjes FJ, Gottschalk M (1991) Characterization of a polygalacturonase gene of Aspergillus niger RH5344. Mol Microbiol 5: 1353-1361
87. Salomov IT, Ashurov AR, Oblakulav KHM, Salomov UI, Dzhalilov BM (1994) Effect of pectin additives from cotton on the physical development, nutrition and erythrocytes in infants. Vopr Pitan 6: 16-18
88. Sanchez LM, Doke N, Kawakita K (1994) Involvement of suppressor-glucans and plant epidermal cells in host-selective pathogenesis of Phytophthora capsici. J Phytopathol 140: 153-164
89. Sanchez JA, Zamorano JP, Alique R (1998) Polygalacturonase, cellulase and invertase activities during cherimoya fruit ripening. J Hortic Sci Biotechnol 73: 87-92
90. Scott-Craig JS, Panaccione DG, Cervone F, Walton JD (1990) Endopolygalacturonase is not required for pathogenicity of Cochliobolus carbonum on maize. Plant Cell 2: 1191-1200
91. Scott-Craig JS, Cheng YQ, Cervone F, De Lorenzo G, Pitkin JW, Walton JD (1998) Targeted mutants of Cochliobolus carbonum lacking the two major extracellular polygalacturonases. Appl Environ Microbiol 64: 1497-1503
92. Selveraj Y, Pal DK, Sing R, Roy TK (1995) Biochemistry of uneven ripening in Gulabi grape. J Food Biochem 18: 325-340
93. Shieh M, Brown RL, Whitehead MP, Cary JW, Cotty PJ, Cleveland TE, Dean RA (1997) Molecular genetic evidence for the involvement of a specific polygalacturonase, P2c, in the invasion and spread of Aspergillus flavus in cotton balls. Appl Environ Microbiol 63: 3548-3552
94. Siekstele R, Bartkeviciute D, Sasnauskas K (1999) Cloning, targeted disruption and heterologous expression of the Kluyveromyces marxianus endopolygalacturonase gene. Yeast 15: 311-322
95. Siessere V, Vieira Fonseca MJ, Said S (1992) Extracellular polygalacturonases from Penicillium frequentans: separation and regulatory aspects. J Gen Microbiol 138: 1801-1805
96. Simpson SD, Ashford DA, Harvey DJ, Bowles DJ (1998) Short chain oligogalacturonides induce ethylene production and expression of the gene encoding aminocyclopropane 1-carboxylic acid oxidase in tomato plants. Glycobiology 8: 579-583
97. Sozzi-Quiroga GO, Fraschina AA (1997) Evaluation of sensory attributes and biochemical parameters in transgenic tomato fruit with reduced polygalacturonase activity. Food Sci Technol Int 3: 93-102
98. Sriram S, Raguchander T, Vidhyasekaran P, Muthukrishan S, Samiyappan R (1997) Genetic relatedness with special reference to virulence among the isolates of Rhizoctonia solani causing sheath blight in rice. J Plant Dis Prot 104: 260-271
99. Staples RC, Meyer AM (1995) Putative virulence factors of Botrytis cinerea acting as a wound pathogen. FEMS Microbiol Lett 134: 1-7
100. Stratilova E, Markovic O, Skrovinova D, Rexova-Benkova L, Jornvall H (1993) Pectinase Aspergillus sp. polygalacturonase: multiplicity, divergence, and structural patterns linking fungal, bacterial, and plant polygalacturonases. J Protein Chem 12: 15-22
101. Stratilova E, Dzurova M, Markovic O, Jornvall H (1996) An essential tyrosine residue of Aspergillus polygalacturonase. FEBS Lett 382: 164-166
102. Stratilova E, Mislovicova D, Kacurakova M, Machova E, Kolarova N, Markovic O, Jornvall H (1998) The glycoprotein character of multiple forms of Aspergillus polygalacturonase. J Protein Chem 17: 173-179
103. Suykerbuyk MEG, Schaap PJ, Stam H, Musters W, Visser J (1995) Cloning, sequence and expression of the gene coding for rhamnogalacturonase of Aspergillus aculeatus: a novel pectinolytic enzyme. Appl Microbiol Biotechnol 43: 861-870
104. Takasawa T, Sagisaka K, Yagi K, Uchiyama K, Aoki A, Takaoka K, Yamamato K (1997) Polygalacturonase isolated from the culture of the psychrophilic fungus Sclerotinia borealis. Can J Microbiol 43: 417-424
105. Taylor MA, Rabe E, Dodd MC, Jacobs G (1994) Effect of storage regimes on pectolytic enzymes, pectic substances, internal conductivity and gel breakdown in cold stored Songold plums. J Hortic Sci 69: 527-534
106. Tenberge KB, Homann V, Oeser B, Tudzynski P (1996) Structure and expression of two polygalacturonase genes of Claviceps purpurea oriented in tandem and cytological evidence for pectinolytic enzyme activity during infection on rye. Phytopathology 86: 1084-1097
107. Ten Have A, Mulder W, Visser J, Van Kan JAL (1998) The endopolygalacturonase gene Bcpg1 is required for full virulence of Botrytis cinerea. Mol Plant Microbe Interact 11: 1084-1097
108. Torki M, Manadaron P, Thomas F, Quigley F, Mache R, Falconet D (1999) Differential expression of a polygalacturonase gene family in Arabidopsis thaliana. Mol Gen Genet 261: 948-952
109. Valsangiacomo C, Ruckstuhl M, Gessler C (1992) In vitro degradation of cell walls of apple leaves by pectinolytic enzymes of the scab fungus, Venturia inaequalis, and by commercial pectinolytic and cellulolytic enzyme preparations. J Phytopathol 135: 20-27
110. Voragen AGJ (1998) Technological aspects of functional food-related carbohydrates. Trends Food Sci Technol 9: 328-335
111. Whitehead MP, Shieh MT, Cleveland TE, Cary JW, Dean RA (1995) Isolation and characterization of polygalacturonase genes (pecA and pecB) from Aspergillus flavus. Appl Environ Microbiol 61: 3316-3322
112. Wubben JP, Mulder W, Ten Have A, Van Kan JAL, Visser J (1999) Cloning and partial characterization of endopolygalacturonase genes from Botrytis cinerea. Appl Environ Microbiol 65: 1596-1602
113. Yamada H (1995) Structure and pharmacological activity of pectic polysaccharides from the roots of Bupleurum falcatum L. Nippon Yakurigaku Zasshi 106: 229-237
114. Yamada H, Hirano M, Kiyohara H (1991) Partial structure of an anti-ulcer pectic polysaccharide from the roots of Bupleurum falcatum L. Carbohydr Res 219: 173-192
115. Yang Y, Bergmann C, Benen J, Orlando R (1997) Identification of the glycosylation site and glycan structures of recombinant endopolygalacturonase II by mass spectrometry. Rapid Commun Mass Spectrom 11: 1257-1262
116. Zhang Z, Pierce ML, Mort AJ (1996) Detection and differentiation of pectic enzyme activity in vitro and in vivo by capillary electrophoresis of products from fluorescent-labeled substrate. Electrophoresis 17: 372-378