Cloning and partial characterization of endopolygalacturonase genes from Botrytis cinema

Applied and Environmental Microbiology

Volumn 65, Issue 4, 1999, Pages 1596-1602

  Wubben, J P ^a   Mulder, W ^a   Have, A Ten ^a   Van Kan, J A L ^a   Visser, J ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

[No Author keywords available]

Indexed keywords

POLYGALACTURONASE;

ARTICLE; BOTRYTIS CINEREA; GENE EXPRESSION REGULATION; GENE ISOLATION; MOLECULAR CLONING; MYCELIUM; NONHUMAN; NUCLEOTIDE SEQUENCE; PHYLOGENY; SPECIES DIFFERENCE;

BACTERIA (MICROORGANISMS); BOTRYOTINIA FUCKELIANA; BOTRYTIS; BOTRYTIS CINEREA; FUNGI;

EID: 0032949792     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.65.4.1596-1602.1999     Document Type: Article

References (43)

1. Annis, S. L., and P. H. Goodwin. 1997. Recent advances in the molecular genetics of plant cell wall-degrading enzymes produced by plant pathogenic fungi. Eur. J. Plant Pathol. 103:1-14.
2. Benen, J. A. E., H. C. M. Kester, and J. Visser. 1999. Kinetic characterization of Aspergillus niger N400 endopolygalacturonases I, II and C. Eur. J. Biochem. 259:577-585.
3. Benen, J. A. E., L. Pařenicová, M. Kusters van Someren, H. C. M. Kester, and J. Visser. 1996. Molecular genetic and biochemical aspects of pectin degradation in Aspergillus, p. 331-346. In J. Visser and A. G. J. Voragen (ed.), Pectins and pectinases. Elsevier Science B.V., Amsterdam, The Netherlands.
4. Bussink, H. J. D., K. B. Brouwer, L. H. De Graaff, H. C. M. Kester, and J. Visser. 1991. Identification and characterization of a second polygalacturonase gene of Aspergillus niger. Curr. Genet. 20:301-308.
5. Bussink, H. J. D., F. P. Buxton, B. A. Fraaye, L. H. De Graaff, and J. Visser. 1992. The polygalacturonases of Aspergillus niger are encoded by a family of diverged genes. Eur. J. Biochem. 208:83-90.
6. Bussink, H. J. D., F. P. Buxton, and J. Visser. 1991. Expression and sequence comparison of the Aspergillus niger and Aspergillus tubingensis genes encoding polygalacturonase II. Curr. Genet. 19:467-474.
7. Bussink, H. J. D., H. C. M. Kester, and J. Visser. 1991. Molecular cloning, nucleotide sequence and expression of the gene encoding prepro-polygalacturonase II of Aspergillus niger. FEBS lett. 273:127-130.
8. Caprari, C., A. Richter, C. Bergmann, S. Lo Cicero, G. Salvi, F. Cervone, and G. De Lorenzo. 1993. Cloning and characterization of a gene encoding the endopolygalacturonase of Fusarium moniliforme. Mycol. Res. 97:497-505.
9. Cary, J. W., R. Brown, T. E. Cleveland, M. P. Whitehead, and R. A. Dean. 1995. Cloning and characterization of a novel polygalacturonase-encoding gene from Aspergillus parasiticus. Gene 153:129-133.
10. Centis, S., B. Dumas, J. Fournier, M. Marolda, and M. T. Esquerré Tugayé. 1996. Isolation and sequence analysis of clpg1, a gene coding for an endopolygalacturonase of the phytopathogenic fungus Colletotrichum lindemuthianum. Gene 170:125-129.
11. Centis, S., I. Guillas, N. Sejalon, M. T. Esquerré Tugayé, and B. Dumas. 1997. Endopolygalacturonase genes from Colletotrichum lindemuthianum: cloning of clpg2 and comparison of its expression to that of clpg1 during saprophytic and parasitic growth of the fungus. Mol. Plant-Microbe Interact. 10:769-775.
12. Di Pietro, A., and M. I. G. Roncero. 1998. Cloning, expression, and role in pathogenicity of pg1 encoding the major extracellular endopolygalacturonase of the vascular wilt pathogen Fusarium oxysporum. Mol. Plant-Microbe Interact. 11:91-98.
13. Edlich, W., G. Lorenz, H. Lyr, E. Nega, and E.-H. Pommer. 1989. New aspects on the infection mechanism of Botrytis cinerea Pers. Neth J. Plant Pathol. 95:53-62.
14. Fickett, J. W. 1982. Recognition of protein coding regions in DNA sequences. Nucleic Acids Res. 10:5303-5318.
15. Fraissinet Tachet, L., P. Reymond Cotton, and M. Fèvre. 1995. Characterization of a multigene family encoding an endopolygalacturonase in Sclerotinia sclerotiorum. Curr. Genet. 29:96-99.
16. Gao, S., G. H. Choi, L. Shain, and D. L. Nuss. 1996. Cloning and targeted disruption of enpg-1, encoding the major in vitro extracellular endopolygalacturonase of the chestnut blight fungus. Cryphonectria parasitica. Appl. Environ. Microbiol. 62:1984-1990.
17. Iguchi, K. I., H. Hirano, M. Kishida, H. Kawasaki, and T. Sakai. 1997. Cloning of a protopectinase gene of Trichosporon penicillatum and its expression in Saccharomyces cerevisiae. Microbiology 143:1657-1664.
18. Jarvis, W. R. 1977. Botryotinia and Botrytis species: taxonomy, physiology, and pathogenicity: a guide to the literature. Canada Department of Agriculture, Ottawa, Ontario, Canada.
19. Johnston, D. J., and B. Williamson. 1992. An immunological study of the induction of polygalacturonases in Botrytis cinerea. FEMS Microbiol. Lett. 97:19-24.
20. Kester, H. C. M., and J. Visser. 1990. Purification and characterization of polygalacturonases produced by the hyphal fungus Aspergillus niger. Biotechnol. Appl. Biochem. 12:150-160.
21. Kitamoto, N., T. Kimura, Y. Kito, K. Ohmiya, and N. Tsukagoshi. 1993. Structural features of a polygalacturonase gene cloned from Aspergillus oryzae KBN616. FEMS Microbiol. Lett. 111:37-41.
22. Leone, G. 1992. Significance of polygalacturonase production by Botrytis cinerea in pathogenesis, p. 63-68. In K. Verhoeff, N. E. Malathrakis, and B. Williamson (ed.), Recent advances in Botrytis research. Pudoc Scientific Publishers, Wageningen, The Netherlands.
23. Leone, G., E. A. M. Schoffelmeer, and J. Van den Heuvel. 1990. Purification and characterization of a constitutive polygalacturonase associated with the infection process of French bean leaves by Botrytis cinerea. Can. J. Bot. 68:1921-1930.
24. Miyairi, K., M. Senda, M. Watanabe, Y. Hasui, and T. Okuno. 1997. Cloning and sequence analysis of cDNA encoding endopolygalacturonase 1 from Stereum purpureum. Biosci. Biotechnol. Biochem. 61:655-659.
25. Möller, E. M., G. Bahnweg, H. Sandermann, and H. H. Geiger. 1992. A simple and efficient protocol for isolation of high molecular weight DNA from filamentous fungi, fruit bodies, and infected plant tissues. Nucleic Acids Res. 20:6115-6116.
26. Movahedi, S., and J. B. Heale. 1990. The roles of aspartic proteinase and endo-pectin lyase enzymes in the primary stages of infection and pathogenesis of various host tissues by different isolates of Botrytis cinerea Pers ex. Pers. Physiol. Mol. Plant Pathol. 36:303-324.
27. Nielsen, H., J. Engelbrecht, S. Brunak, and G. von Heijne. 1997. Identification of prokaryotic and eukaryotic signal peptides and prediction of cleavage sites. Protein Eng. 10:1-6.
28. Pařenicová, L. 1998. Unpublished results.
29. Pařenicovà, L., J. A. E. Benen, H. C. M. Kester, and J. Visser. 1998. pgaE encodes a fourth member of the endopolygalacturonase gene family from Aspergillus niger. Eur. J. Biochem. 251:72-80.
30. Reignault, P., M. Mercier, G. Bompeix, and M. Boccara. 1994. Pectin methylesterase from Botrytis cinerea: physiological, biochemical and immunochemical studies. Microbiology 140:3249-3255.
31. Reymond, P., G. Deléage, C. Rascle, and M. Fèvre. 1994. Cloning and sequence analysis of a polygalacturonase-encoding gene from the phytopathogenic fungus Sclerotinia sclerotiorum. Gene 146:233-237.
32. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, N.Y.
33. Scott-Craig, J. S., D. G. Panaccione, F. Cervone, and J. D. Walton. 1990. Endopolygalacturonase is not required for pathogenicity of Cochliobolus carbonum on maize. Plant Cell 2:1191-1200.
34. Staples, R. C., and A. M. Mayer. 1995. Putative virulence factors of Botrytis cinerea acting as a wound pathogen. FEMS Microbiol. Lett. 134:1-7.
35. Swofford, D. L. 1993. PAUP: phylogenetic analysis using parsimony version 3.1.1. Illinois Natural History Survey, Champaign, III.
36. Tenberge, K. B., V. Homann, B. Oeser, and P. Tudzynski. 1996. Structure and expression of two polygalacturonase genes of Claviceps purpurea oriented in tandem and cytological evidence for pectinolytic enzyme activity during infection of rye. Phytopathology 86:1084-1097.
37. Ten Have, A. 1998. Unpublished results.
38. Ten Have, A., W. Mulder, J. Visser, and J. A. L. Van Kan. 1998. The endopolygalacturonase gene Bcpg1 is required for full virulence of Botrytis cinerea. Mol. Plant-Microbe Interact. 11:1009-1016.
39. Unkles, S. E. 1992. Gene organization in filamentous fungi, p. 28-53. In J. R. Kinghorn and G. Turner (ed.), Applied molecular genetics of filamentous fungi. Blackie Academic and Professional, Glasgow, Scotland.
40. Van der Cruyssen, G., E. De Meester, and O. Kamoen. 1994. Expression of polygalacturonases of Botrytis cinerea in vitro and in vivo. Meded. Fac. Landbouwkd. Toegep. Biol. Wet. Univ. Gent 59:895-905.
41. Van der Vlugt Bergmans, C. J. B., C. A. M. Wagemakers, and J. A. L. Van Kan. 1997. Cloning and expression of the cutinase A gene of Botrytis cinerea. Mol. Plant-Microbe Interact. 10:21-29.
42. Whitehead, M. P., M. T. Shieh, T. E. Cleveland, J. W. Cary, and R. A. Dean. 1995. Isolation and characterization of polygalacturonase genes (pecA and pecB) from Aspergillus flavus. Appl. Environ. Microbiol. 61:3316-3322.
43. Yang, Y., C. Bergmann, J. Benen, and R. Orlando. 1997. Identification of the glycosylation site and glycan structures of recombinant endopolygalacturonase II by mass spectrometry. Rapid Commun. Mass Spectrom. 11:1257-1262.