Primary structure and characterization of an exopolygalacturonase from Aspergillus tubingensis

European Journal of Biochemistry

Volumn 240, Issue 3, 1996, Pages 738-746

  Kester, Harry C M ^a   Kusters Van Someren, Margo A ^a   Müller, Yvonne ^a   Visser, Jaap ^a  

^a WAGENINGEN UNIVERSITY   (Netherlands)

Author keywords

Aspergillus; Exopolygalacturonase; Gene expression; Kinetic analysis; Subsite structure

Indexed keywords

GALACTURAN 1,4 ALPHA GALACTURONIDASE; GALACTURONIC ACID; POLYGALACTURONASE; UNCLASSIFIED DRUG;

AMINO ACID SEQUENCE; ARTICLE; ASPERGILLUS; ASPERGILLUS NIGER; ENZYME ACTIVITY; ENZYME INDUCTION; ENZYME PURIFICATION; ENZYME STRUCTURE; GENE EXPRESSION; MOLECULAR CLONING; MOLECULAR WEIGHT; PRIORITY JOURNAL; SEQUENCE HOMOLOGY;

ASPERGILLUS; ASPERGILLUS NIGER; ASPERGILLUS TUBINGENSIS; FUNGI;

EID: 0029778186     PISSN: 00142956     EISSN: None     Source Type: Journal    
DOI: 10.1111/j.1432-1033.1996.0738h.x     Document Type: Article

References (49)

1. Ahmem El Rayah, A. & Labavitch, J. M. (1977) A simplified method for accurate determination of cell wall uronide content, J. Food Biochem. 1, 361-365.
2. Azadi, P., O'Neill, M., Bergmann, C., Darvill, A. G. & Albersheim, P. (1995) The backbone of the pectic polysaccharide rhamnogalacturonan I is cleaved by an endohydrolase and an endolyase, Glycobiology 5, 783-789.
3. Bailey, C. & Arst, H. Jr (1975) Carbon catabolite repression in Aspergillus midulans, Eur. J. Biochem. 51, 573-577.
4. Bussink, H. J. D., Buxton, F. P. & Visser, J. (1991a) Expression and sequence comparison of the Aspergillus niger and Aspergillus tubingensis genes encoding polygalacturonase-II, Curr. Gen. 19, 467-474.
5. Bussink, H. J. D., Brouwer, K. B., de Graaff, L. H., Kester, H. C. M. & Visser, J. (1991b) Identification and characterization of a second polygalacturonase gene of Aspergillus niger, Curr. Genet. 20, 301-307.
6. Bussink, H. J. D., Buxton, F. P., Fraaye, B. A., de Graaff, L. H. & Visser, J. (1992) The polygalacturonases of Aspergillus niger are encoded by a family of diverged genes, Eur. J. Biochem. 208, 83-90.
7. Cooke, R. D., Ferber, C. E. M. & Ranagasabapathy, L. (1976) Purification and characterisation of polygalacturonases from a commercial Aspergillus niger preparation, Biochim. Biophys. Acta 452, 440-451.
8. Cubero, B. & Scazzocchio, C. (1994) Two different adjacent and divergent zinc finger binding sites are necessary for CREA-mediated carbon catabolite repression in the proline gene cluster of Aspergillus nidulans, EMBO J. 13, 407-415.
9. de Graaff, L., van den Broek, H. & Visser, J. (1988) Isolation and transformation of the pyruvate kinase gene of Aspergillus nidulans, Curr. Genet. 13, 315-321.
10. de Graaff, L., van den Broeck, H. & Visser, J. (1992) Isolation and characterization of the Aspergillus niger pyruvate kinase gene, Curr. Genet. 22, 21-27.
11. de Graaff, L. H., van den Broeck, H. C., van Ooijen, A. J. J. & Visser, J. (1994) Regulation of the xylanase-encoding xlnA gene of Aspergillus tubingensis, Mol. Microbiol. 12, 479-490.
12. Dente, L., Cesarini, G. & Cortese, R. (1983) pEMBL: a new family of single stranded plasmids, Nucleic Acids Res. 11, 1645-1655.
13. de Vries, J., Voragen, A. G. F., Rombouts, F. M. & Pilnik, W. (1986) in Recent advances in the chemistry and function of pectins (Fisherman, M. L. & Jen, J. J., eds) vol. 310, pp. 38-48. ACS Symposium series, American Chemical Society, Washington DC.
14. Espeso, E. A. & Peñalva, M. A. (1994) In vitro binding of the two finger repressor CreA to several consensus and non-consensus sites at the ipna upstream region is context dependent, FEBS Lett. 342, 43-48.
15. Felsenstein, J. (1989) PHYLIP-Phylogeny Inference Package (Version 3.2), Cladistics 5, 164-166.
16. Flipphi, M. J. A., Visser, J., Van der Veen, P. & De Graaff, L. H. (1994) Arabinase gene expression in Aspergillus: Indications for coordinated regulation, Microbiology 140, 2673-2682.
17. Goosen, T., Bloemheuvel, G., Gysler, C., de Bie, D. A., van den Broek H. W. J. & Swart, K. (1987) Transformation of Aspergillus niger using the homologous orotidine-5′-phosphate-decarboxylase gene, Curr. Genet. 11, 499-503.
18. Guarente, L., Lalonde, B., Gifford, B. & Alani, E. (1984) Distinctly regulated tandem upstream activation sites mediate catabolite repression of the CYC1 gene of S. cerevisiae, Cell 36, 503-511.
19. Hanahan, D. (1983) Studies on transformation of Escherichia coli with plasmids, J. Mol. Biol. 166, 557-580.
20. Hara, T., Lim, J. Y. & Ueda, S. (1984) Purification and some properties of exopolygalacturonase from Aspergillus niger cultured in the medium containing satsuma mandarin peel, J. Jpn. Soc. Food-Sci. Technol. 31, 581-586.
21. Higgins, D. G. & Sharp, P. M. (1988) CLUSTAL: a package for performing multiple sequence alignment on a microcomputer, Gene (Amst.) 73, 237-244
22. Hiromi, K. (1970) Interpretation of dependency of rale parameters on the degree of polymerization of substrate in enzyme-catalyzed reactions. Evaluation of subsite affinities of exoenzymes, Biochem. Biophys. Res. Commun. 40, 1-6.
23. Hiromi, K., Nitta, Y., Numata, C. & Ono, S. (1973) Suhsite affinities of glucoamylase: examination of the validity of the subsite theory, Biochem. Biophys. Acta 302, 362-375.
24. Kester, H. C. M. & Visser, J. (1990) Purification and characterization of polygalacturonases produced by the hyphal fungus Aspergillus niger, Biotech. Appl. Biochem. 12, 150-160.
25. Kita, A. K., Matsui, H., Somoto, A., Kimaru, A., Takata, M. & Chiba, S. (1991) Substrate specificity and subsite affinities of crystalline alpha-glucosidase from Apergillus niger, Agric. Biol. Chem. 55, 2327-2325.
26. Kofod, L. V., Kauppinen, S., Christgau, S., Andersen, L. N., Heldt-Hansen, H. P., Dörreich, K. & Dalboge, U. (1994) Cloning and characterization of two structurally and functionally divergent rhamnogalacturonases from Aspergillus aculeatus, J. Biol. Chem. 269, 29 182-29 189.
27. Kulmburg, P., Mathieu, M., Dowzer, C., Kelly, J. & Felenbok, B. (1993) Specific binding sites in the alcR and alcA promotors of the ethanol regulon for the CRLA repressor mediating carbon catabolite repression in Aspergillus nidulans. Mol. Microbiol. 7, 847-857.
28. Kusters-van Someren, M. A., Samson, R. A. & Visser, J. (1991) The use of RFLP analysis in classification of the black Aspergilli - Reinterpretation of the Aspergillus niger aggregate, Curr. Genet. 19, 21-26.
29. Kusters-van Someren, M. A., Flipphi, M. J. A., de Graaff, L. H., van den Broeck, H. C., Kester, H. C. M., Hinnen, A. & Visser, J. (1992) Characterization of the Aspergillus niger pelB gene: structure and regulation of expression. Mol. Gen. Genet. 234, 113-120.
30. Laemmli, U. K. (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4, Nature 227, 680-685.
31. Matsudaira, P. (1990) Limited N-terminal sequence analysis, Methods Enzymol. 182, 602-613.
32. Mill, P. J. (1996a) The pectic enxymes of Aspergillus niger. A mercury-activated exopolygalacturonase. Biochem. J. 99, 557-561.
33. Mill, P. J. (1966b) The pectic enzymes of Aspergillus niger. A second exopolygalacturonase. Biochem. J. 99, 562-565.
34. Omran, H., Dörreich, K. & Gierscher, K. (1986) Some results of the isolation of discrete enzymes (exopolygalacturonase and pectinesterase) from a commercial pectic enzyme preparation. 1. Isolation, purification and characterisation of an exopolygalacturonase (exoPG). Lebensm.-Wiss. u. Technol. 19, 457-463.
35. Rambosek, J. & Leach, J. (1987) Recombinant UNA in filamentous fungi: progress and prospects. CRC Crit. Rev. Biotech. 6, 357-393.
36. Rexová-Benková, L. (1990) Evidence for the role of carboxyl groups in activity of endopolygalacturonase of Aspergillus niger. Chemical modification by carbodiimide reagent, Collect. Czech. Chem. Commun. 55, 1389-1395.
37. Rexová-Benková, L. & Mraçková, M. (1978) Active groups ot extracellular endoD-galacturonase of Aspergillus niger derived from pH effect on kinetic data, Biochim. Biophys. Acta 523, 162-169.
38. Rexová-Benková, L. & Slezárik, A. (1970) On the role of histidine in the active center of the endopolygalacturonase of Aspergilus niger, Coll. Czech. Chem. Comm. 35, 1255-1260.
39. Robyt, J. F., Ackerman, R. J. & Keng, J. G. (1973) Reducing value methods for maltodextrins: II. Automated methods and chain-length independence of alkaline ferricyanide, Anal. Biochem. 45, 517-524.
40. Rolin, C. & de Vries, J. (1990) in Food gels (Harris, P., ed.), pp. 401-434. Elsevier Applied Science, London and New York.
41. Rombouts, F. M. & Pilnik, W. (1980) in Microbiol enzymes and bioconversions (Rose, A. H., ed.), vol. 5, pp. 227-282. Economic Microbiology, Academic Press, London.
42. Sambrook, J., Fritsch, E. F. & Maniatis, T. (1989) Molecular cloning: a laboratory manual, Cold Spring Harbor, New York. Cold Spring Harbor Laboratory Press.
43. Schols, H. A., Geraeds, C. C. J. M., Searle-van Leeuwen, M. F., Kormelink, F. J. M. & Voragen, A. G. J. (1990a) Rhamnogalacturonase: a novel enzyme that degrades the hairy regions of pectins. Carbohydr. Res. 206, 105-115.
44. Schols, H. A., Posthumus, M. A. & Voragen, A. G. J. (1990b) Structural features of hairy regions of pectins isolated from apple juice produced by the liquefaction process, Carbohydr. Res. 206, 117-129.
45. Stephens, B. G., Felkel, H. J. Jr & Spinelli, W. M. (1974) Spectrophotometric determination of copper and iron subsequent to the simultaneous extraction of bis(2,9-dimethyl-1,O-phenantroline)copper(I) and bis[2,4,6-tri(2-pyridyl)-1,3,5-triazine]iron(II) into propylene carbonate, Anal. Chem. 46, 692-696.
46. Suykerbuyk, M. E. G., Schaap, P. J., Stam, H., Musters, W. & Visser, J. (1995) Cloning, sequence and expression of the gene coding for rhamnogalacturonase of Aspergillus aculeatus; a novel pectinolytic enzyme, Appl. Microbiol. Biotechnol. 43, 861-870.
47. Vishniae, W. & Santer, M. (1957) The thiobacilli, Bacteriol. Rev. 21, 195-213.
48. von Heijne, G. (1986) A new method for predicting signal sequence cleavage sites, Nucleic Acids Res. 14, 4683-4690.
49. Whitaker, J. R. (1990) in Microbial enzymes and biotechnology (Fogarty, W. M. & Kelly, C. T., eds) 2nd edn, pp. 133-176, Elsevier Applied Science, London and New York.