Cloning, sequence analysis and overexpression of a Saccharomyces cerevisiae endopolygalacturonase-encoding gene (PGL1)

Yeast

Volumn 15, Issue 1, 1999, Pages 11-22

  Gognies, S ^a   Gainvors, A ^a   Aigle, M ^b   Belarbi, A ^a  

^a UNIVERSITÉ DE REIMS CHAMPAGNE ARDENNE   (France)

^b Institut de Biochimie et Genetique Cellulaires   (France)

Author keywords

Pectin; Pectinases; PGL1 gene; Polygalacturonase; Yeast

Indexed keywords

AGAR; ESTERASE; GALACTURONIC ACID; HYDROLASE; LYASE; PECTIN; POLYGALACTURONASE; RUTHENIUM RED;

AMINO ACID SEQUENCE; ARTICLE; CHEMICAL BOND; DEPOLYMERIZATION; ENZYME ACTIVITY; GENE OVEREXPRESSION; MOLECULAR CLONING; NONHUMAN; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SEQUENCE ANALYSIS;

AMINO ACID SEQUENCE; BASE SEQUENCE; CLONING, MOLECULAR; DNA, FUNGAL; GENE EXPRESSION; GENES, FUNGAL; MOLECULAR SEQUENCE DATA; PECTINS; PLASMIDS; POLYGALACTURONASE; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

EUKARYOTA; FUNGI; MYXOGASTRIA; SACCHAROMYCES CEREVISIAE;

EID: 0033555443     PISSN: 0749503X     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1097-0061(19990115)15:1<11::AID-YEA336>3.0.CO;2-O     Document Type: Article

References (52)

1. Atkinson, R. G. (1994). A cDNA clone for endopolygalacturonase from apple. Plant Physiol. 105, 1437-1438.
2. Atkinson, R. G. and Gardner, R. C. (1993). A polygalacturonase gene from kiwifruit (Actinidia deliciosa) Plant Physiol. 103, 669-670.
3. Baganz, F., Hayes, A. and Oliver, S. G. (1997). A quantitative approach to the analysis of gene function, using competition experiments to measure selective fitness. In Eighteenth International Conference on Yeast Genetics and Molecular Biology, Stellenbosch, South Africa. Yeast 13, 183.
4. Barnby, F. M., Morpeth, F.F. and Pyle, D. L. (1990). Endopolygalacturonase production from Kluyveromyces marxianus. Resolution, purification, and partial characterization of the enzyme . Enzyme Microbiol. Technol. 12, 891-897.
5. Baron, A. and Drilleau, J. F. (1982). Utilisation de la pectinestérase dans les industries cidricoles. Utilisation des enzymes en technologie alimentaire. In Dupuy, P. (Ed.), Lavoisier, Paris, pp. 471-476.
6. Be Miller, J. N. (1986). An introduction to pectins: structure and properties. In Fishman, M. L. and Jen, J. J. (Eds), Chemistry and Function of Pectins. ACS Symposium Series 310, Washington, DC, pp. 2-12.
7. Becker, D. M. and Guarente, L. (1992). High efficiency transformation of yeast by electroporation. Meth. Enzymol. 194, 182..
8. Blanco, P., Sieiro, C., Reboredo, N. M. and Villa, T. G. (1997). Genetic determination of polygalacturonase production in wild-type and laboratory strains of Saccharomyces cerevisiae. Arch. Microbiol. 167, 284-288.
9. Brown, M. R. and Ough, C. S. (1981). A comparison of activity and effects of two commercial pectic enzyme preparations on white grape musts and wines. Am. J. Vitic. Enol. 32, 272-276.
10. Bussink H. J., Kester, H. C. and Visser, J. (1990). Molecular cloning, nucleotide sequence and expression of the gene encoding prepro-polygalacturonase II of Aspergillus niger. FEBS Lett. 273, 127-130.
11. Cary, J. W. Brown, R., Cleveland, T. E., Whitehead, M. and Dean, R. A. (1995). Cloning and characterization of a novel polygalacturonase-encoding gene from Aspergillus parasiticus. Gene 153, 129-133.
12. Clewell, D. B. and Helsinki, D. R. (1969). Supercoiled circular DNA protein complex in Escherichia coli: purification and induced conversion to an opened circular DNA form. Proc. Natl. Acad. Sci. USA 62, 1159-1166.
13. Cohen, S. N., Chang, A. C. Y. and Hsu, L. (1972). Non-chromosomal antibiotic resistance in bacteria: genetic transformation of E. coli by R factor DNA. Proc. Natl. Acad. Sci. USA 69, 2110-2114.
14. Cruickshank, R. H. and Wade, G. C. (1980). Detection of pectic enzymes in pectin-acrylamide gels. Anal. Biochem. 107, 177-181.
15. De Vries, J. A., Vorajen, A. G. J., Rombouts, F. M. and Pilnik, W. (1986). In Fisherman, M. L. and Jen, J. J. (Eds), Chemistry and Function of Pectins. American Chemical Society, Washington, DC.
16. Depiereux, E. and Feytmans, E. (1992). Match-Box: a fundamentally new algorithm for the simultaneous alignment of several protein sequences. CABIOS 8, 501-509.
17. DeRisi, J L., Iyer, V. R. and Brown, P. O. (1997). Exploring the metabolic and genetic control of gene expression on a genomic scale. Science 278, 680-686.
18. Dopico, B., Lowe, A. L., Wilson, I. D., Merodio, C. and Grierson, D. (1993). Cloning and characterization of avocado fruit mRNAs and their expression during ripening and low-temperature storage. Plant Mol. Biol. 21, 437-449.
19. Dujon, B. (1996). The yeast genome project: what did we learn? Trends Genet. 12, 263-269.
20. Gainvors, A. and Belarbi, A. (1995). Detection method for polygalacturonase-producing strains of Saccharomyces cerevisiae. Yeast 11, 1493-1499.
21. Gainvors, A., Frézier, V., Lemaresquier, H., Lequart, C., Aigle, M. and Belarbi, A. (1994a). Detection of polygalacturonase, pectin-lyase and pectin-esterase in a Saccharomyces cerevisiae. Yeast 10, 1311-1319.
22. Gainvors, A., Karam, N., Lequart, C. and Belarbi, A. (1994b). Use of Saccharomyces cerevisiae for the clarification of fruit juices. Biotechnol. Lett. 16, 1329-1334.
23. Gonzalez-Candelas, L., Cortell, A. and Ramon, D. (1995). Construction of a recombinant wine yeast strain expressing a fungal pectate lyase gene. FEMS Microbiol. Lett. 126, 263-270.
24. Hinton, J. C., Gill, D. R., Lalo, D., Plastow, G. S. and Salmond, G. P. (1990). Sequence of the peh gene of Envinia carotovora: homology between Erwinia and plant enzymes. Mol. Microbiol. 4, 1029-1036.
25. Holmes, D. S. and Quigley, M. (1981). A rapid boiling method for the preparation of bacterial plasmids. Anal. Biochem. 114, 193-197.
26. Huang, J. H. and Schell, M. A. (1990). DNA sequence analysis of pg1A and mechanism of export of its polygalacturonase product from Pseudomonas solanacearum. J. Bacteriol. 172, 3879-3887.
27. Karam, N. E. and Belarbi, A. (1995). Detection of polygalacturonases and pectin esterases in lactic acid bacteria. World J. Microbiol. Biotechnol. 11, 559-563.
28. Laemmli, U. K. (1970). Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227, 680-685.
29. Laing, E. and Pretorius, I. S. (1992). Synthesis and secretion of an Erwinia chrysanthemi pectate lyase in Saccharomyces cerevisiae regulated by different combinations of bacterial and yeast promoter and signal sequences. Gene 121, 35-45.
30. Laing, E. and Pretorius, I. S. (1993a). A note on the primary structure and expression of an Erwinia carotovora polygalacturonase-encoding gene (peh1) in Escherichia coli and Saccharomyces cerevisiae. J. Appl. Bacteriol. 75, 149-158.
31. Laing, E. and Pretorius, I. S. (1993b). Co-expression of an Erwinia chrysanthemi pectate lyase-encoding gene (pehE) and an E. carotovora polygalacturonase-encoding gene (peh1) in Saccharomyces cerevisiae. Appl. Microbiol. Biotechnol. 3, 181-188.
32. Luh, B. S. and Phaff, H. J. (1951). Studies on polygalacturonase of certain yeasts. Arch. Biochem. Biophys. 33, 212-227.
33. Marck, C. (1988). 'DNA Strider': a 'C' program for the fast analysis of DNA and protein sequences on Apple Macintosh family of computers. Nucl. Acid Res. 16, 1829-1836.
34. McKay, A. M. (1988). A plate assay method for the detection of fungal polygalacturonase secretion. FEMS Microbiol. Lett. 56, 355-358.
35. McKay, A. M. (1990). Degradation of polygalacturonic acid by Saccharomyces cererisiae. Lett. Appl. Microbiol. 11, 41-44.
36. McNeil, M., Darvill, A. G., Fry, S. C. and Albersheim, P. (1984). Structure and function of the primary cell wall of plants. Ann. Rev. Biochem. 53, 625-663.
37. Milner, Y. and Avigad, G. (1967). A copper reagent for the determination of hexuronic acids and certain ketohexoses. Carbohydr. Res. 4, 359-361.
38. Nelson, N. (1944). A photometric adaptation of the Sogomyi method for the determination of glucose. J. Biol. Chem. 153, 375.
39. Oliver S. (1996). A network approach to the systematic analysis of yeast gene function. TIG. 12, 240-277.
40. Pilnik, W. (1982) Enzymes in the beverage industry (fruit juices, nectar, wine, spirits and beer). In Use of Enzymes in Food Technology, Versailles, May 5-7. CNERNA (CNRS), pp. 425-451.
41. Pilnik, W. and Rombouts, F. M. (1985). Polysaccharides and food processing. Carbohydr. Res. 142, 93-105.
42. Potier, S (1986). Translocation réciproque entre sites chromosomiques choisis. Remplacement du locus URA2 sauvage par des allèles délétes in vitro chez Saccharomyces cerevisiae. Thèse d'Etat. Université de Strasbourg 1.
43. Rombouts, F. M. and Pilnik, W. (1978). Enzymes in fruit and vegetable juice technology. Proc. Biochem. 8, 9-13.
44. Ruttkowski, E., Labitzke, R., Khanh, N. Q., Loffler, F., Gottschalk, M. and Jany, K. D. (1990). Cloning and DNA sequence analysis of a polygalacturonase cDNA from Aspergillus niger RH5344. Biochim. Biophys. Acta 1087, 104-106.
45. Rzedowski, W. (1972). Utilisation des enzymes dans l'industrie des fruits et légumes. Ann. Technol. Agric. 21, 535-543.
46. Saarilahti, H. T., Heino, P., Pakkanen, R., Kalkkinen, N., Palva, I. and Palva, E. T. (1990). Structural analysis of the pehA. gene and characterization of its protein product, endopolygalacturonase, of Erwinia carotovora subspecies carotovora. Mol. Microbiol. 4, 1037-1044.
47. Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Muilis, K. B. and Erlich, H. A. (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239, 487-491.
48. Van Rensburg P., Van Zyl, W. H. and Pretorius, I. S. (1994). Expression of the Butyrivibrio fibrisolvens endo-β-1,4-glucanase gene together with the Erwinia pectate lyase and polygalacturonase genes in Saccharomyces cerevisiae. Curr. Genet. 27, 17-22.
49. Vernet, T., Dignard, D. and Thomas, D. Y. (1987). A family of yeast expression vectors containing the phage f1 intergenic region. Gene 52, 225-233.
50. Von Heijne, G. (1986). A new method for predicting signal sequence cleavage sites. Nucl. Acids Res. 14, 4683-4691.
51. Winston, F., Chumley, F. and Fink, G. R. (1983). Eviction and transplacement of mutant genes in yeast. Meth. Enzymol. 101, 211-228.
52. Yamasaki, M., Kato, A., Chu, S.-Y. and Arima, K. (1967). Pectic enzymes in the clarification of apple juice. Part II. The mechanism of clarification. Agric. Biol. Chem. 31, 552-560.