Effects of amino acid substitutions at position 115 on the fidelity of human immunodeficiency virus type 1 reverse transcriptase

Journal of Virology

Volumn 74, Issue 14, 2000, Pages 6494-6500

  Boyer, Paul L ^a   Hughes, Stephen H ^a  

^a NATIONAL CANCER INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

RNA DIRECTED DNA POLYMERASE;

AMINO ACID SUBSTITUTION; ARTICLE; DNA SYNTHESIS; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME ASSAY; HUMAN IMMUNODEFICIENCY VIRUS 1; NONHUMAN; PRIORITY JOURNAL; VIRUS REPLICATION;

AMINO ACID SEQUENCE; AMINO ACID SUBSTITUTION; BASE PAIR MISMATCH; BASE SEQUENCE; BETA-GALACTOSIDASE; DNA, BACTERIAL; ESCHERICHIA COLI; HIV-1 REVERSE TRANSCRIPTASE; MOLECULAR SEQUENCE DATA; MUTATION, MISSENSE;

EID: 0033934724     PISSN: 0022538X     EISSN: None     Source Type: Journal    
DOI: 10.1128/JVI.74.14.6494-6500.2000     Document Type: Article

References (26)

1. Abbots, J., K. Bebenek, and T. A. Kunkel. 1993. Mechanism of HIV-1 reverse transcriptase. Termination of processive synthesis on a natural DNA template is influenced by the sequence of the template-primer stem. J. Biol. Chem. 268:10312-10323.
2. Arion, D., N. Kaushik, S. McCormick, G. Borkow, and M. A. Parniak. 1998. Phenotypic mechanism of HIV-1 resistance to 3′-azido-3′-deoxythymidine (AZT): increased polymerization processivity and enhanced sensitivity to pyrophosphate of the mutant viral reverse transcriptase. Biochemistry 37: 15908-15917.
3. Bebenek, K., J. Abbotts, J. D. Roberts, S. H. Wilson, and T. A. Kunkel. 1989. Specificity and mechanism of error-prone replication by human immunodeficiency virus-1 reverse transcriptase. J. Biol. Chem. 264:16948-16956.
4. Bebenek, K., J. Abbotts, S. H. Wilson, and T. A. Kunkel. 1993. Error-prone polymerization by HIV-1 reverse transcriptase. Contribution of template-primer misalignment, miscoding, and termination probability to mutational hot spots. J. Biol. Chem. 268:10324-10334.
5. Bebenek, K., W. A. Beard, J. R. Casas-Finet, H.-R. Kim, T. A. Darden, S. H. Wilson, and T. A. Kunkel. 1995. Reduced frameshift fidelity and processivity of HIV-1 reverse transcriptase mutants containing alanine substitutions in helix H of the thumb subdomain. J. Biol. Chem. 270:19516-19523.
6. Bebenek, K., and T. A. Kunkel. 1991. Analyzing fidelity of DNA polymerases. Methods Enzymol. 262:217-232.
7. Boyer, J. C., K. Bebenek, and T. A. Kunkel. 1996. Analyzing the fidelity of reverse transcription and transcription. Methods Enzymol. 275:523-537.
8. Boyer, J. C., K. Bebenek, and T. A. Kunkel. 1992. Unequal human immunodeficiency virus type 1 reverse transcriptase error rates with RNA and DNA templates. Proc. Natl. Acad. Sci. USA 89:6919-6923.
9. Boyer, P. L., S. G. Sarafianos, E. Arnold, and S. H. Hughes. 2000. Analysis of mutations at positions 115 and 116 in the dNTP binding site of HIV-1 reverse transcriptase. Proc. Natl. Acad. Sci. USA 97:3056-3061.
10. Ding, J., K. Das, Y. Hsiou, S. G. Sarafianos, A. D. Clark, Jr., A. Jacobo-Molina, C. Tantillo, S. H. Hughes, and E. Arnold. 1998. Structure and functional implications of the polymerase active site region in a complex of HIV-1 RT with a double-stranded DNA template-primer and an antibody Fab fragment at 2.8 Å resolution. J. Mol. Biol. 284:1095-1111.
11. Drosopoulos, W. C., and V. R. Prasad. 1998. Increased misincorporation fidelity observed for nucleoside analog resistance mutations M184V and E89G in human immunodeficiency virus type 1 reverse transcriptase does not correlate with the overall error rate measured in vitro. J. Virol. 72:4224-4230.
12. Gao, G., M. Orlova, M. M. Georgiadis, W. A. Hendrickson, and S. P. Goff. 1997. Conferring RNA polymerase activity to a DNA polymerase: a single residue in reverse transcriptase controls substrate selection. Proc. Natl. Acad. Sci. USA 94:407-411.
13. Huang, H., R. Chopra, G. L. Verdine, and S. C. Harrison. 1998. Structure of a covalently trapped catalytic complex of HIV-1 reverse transcriptase: implications for drug resistance. Science 282:1669-1675.
14. Hubner, A., M. Kruhoffer, F. Grosse, and G. Krauss. 1992. Fidelity of human immunodeficiency virus type 1 reverse transcriptase in copying natural RNA. J. Mol. Biol. 223:595-600.
15. Ji, J., and L. A. Loeb. 1992. Fidelity of HIV-1 reverse transcriptase copying RNA in vitro. Biochemistry 31:954-958.
16. Kunkel, T. A. 1990. Misalignment-mediated DNA synthesis errors. Biochemistry 29:8003-8011.
17. Kunkel, T. A., and A. Soni. 1988. Mutagenesis by transient misalignment. J. Biol. Chem. 253:14784-14789.
18. Martin-Hernandez, A. M., E. Domingo, and L. Menendez-Arias. 1996. Human immunodeficiency virus type 1 reverse transcriptase: role of Tyr115 in deoxynucleotide binding and misinsertion fidelity of DNA synthesis. EMBO J. 15:4434-4442.
19. Martin-Hernandez, A. M., M. Gutierrez-Rivas, E. Domingo, and L. Menendez-Arias. 1997. Mispair extension fidelity of human immunodeficiency virus type 1 reverse transcriptases with amino acid substitutions affecting Tyr115. Nucleic Acids Res. 25:1383-1389.
20. Meyer, P. R., S. E. Matsuura, A. G. So, and W. A. Scott. 1998. Unblocking of chain-terminated primer by HIV-1 reverse transcriptase through a nucleotide-dependent mechanism. Proc. Natl. Acad. Sci. USA 95:13471-13476.
21. Rezende, L. F., K. Curr, T. Ueno, H. Mitsuya, and V. R. Prasad. 1998. The impact of multidideoxynucleoside resistance-conferring mutations in human immunodeficiency virus type 1 reverse transcriptase on polymerase fidelity and error specificity. J. Virol. 72:2890-2895.
22. Roberts, J. D., K. Bebenek, and T. A. Kunkel. 1988. The accuracy of reverse transcriptase from HIV-1. Science 242:1171-1173.
23. Telesnitsky, A., and S. P. Gof. 1997. Reverse transcription and the generation of retroviral DNA. p. 121-160. In J. M. Coffin, S. H. Hughes, and H. E. Varmus (ed.), Retroviruses. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
24. Tisdale, M., T. Alnadaf, and D. Cousens. 1997. Combination of mutations in human immunodeficiency virus type 1 reverse transcriptase required for resistance to the carbocyclic nucleoside 1592U89. Antimicrob. Agents Chemother. 41:1094-1098.
25. Vartanian, J.-P., U. Plikat, M. Henry, R. Mahieux, L. Guillemot, A. Meyerhans, and S. Wain-Hobson. 1997. HIV genetic variation is directed and restricted by DNA precursor availability. J. Mol. Biol. 270:139-151.
26. Wainberg, M. A., W. C. Drosopoulos, H. Salomon, M. Hsu, G. Borkow, M. Prniak, Z. Gu, Q. Song, J. Manne, S. Islam, G. Castriota, and V. R. Prasad. 1996. Enhanced fidelity of 3TC-selected mutant HIV-1 reverse transcriptase. Science 271:1282-1285.