All seven comG open reading frames are required for DNA binding during transformation of competent Bacillus subtilis

Journal of Bacteriology

Volumn 180, Issue 1, 1998, Pages 41-45

  Chung, Y S ^a   Dubnau, D ^a  

^a PUBLIC HEALTH RESEARCH INSTITUTE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACILLUS SUBTILIS; BACTERIUM TRANSFORMATION; GENE INSERTION; GENE TARGETING; NONHUMAN; OPEN READING FRAME; PRIORITY JOURNAL; PROTEIN ASSEMBLY; PROTEIN DETERMINATION; PROTEIN DNA BINDING;

BACILLUS SUBTILIS; BACTERIA (MICROORGANISMS); NEGIBACTERIA;

EID: 0031973221     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/jb.180.1.41-45.1998     Document Type: Article

References (29)

1. Albano, M., R. Breitling, and D. A. Dubnau. 1989. Nucleotide sequence and genetic organization of the Bacillus subtilis comG operon. J. Bacteriol. 171: 5386-5404.
2. Albano, M., and D. A. Dubnau. 1989. Cloning and characterization of a cluster of linked Bacillus subtilis late competence mutations. J. Bacteriol. 171:5376-5385.
3. Albano, M., J. Hahn, and D. Dubnau. 1987. Expression of competence genes in Bacillus subtilis. J. Bacteriol. 169:3110-3117.
4. Anagnostopoulos, C., and J. Spizizen. 1961. Requirements for transformation in Bacillus subtilis. J. Bacteriol. 81:741-746.
5. Breitling, R., and D. Dubnau. 1990. A membrane protein with similarity to N-methylphenylalanine pilin is essential for DNA binding by competent Bacillus subtilis. J. Bacteriol. 172:1499-1508.
6. Chung, Y. S., and D. Dubnau. 1994. ComC is required for the processing and translocation of ComGC, a pilin-like competence protein of Bacillus subtilis. Mol. Microbiol. 15:543-551.
7. Chung, Y. S., and D. Dubnau. Unpublished data.
8. Clifton, S. W., D. McCarthy, and B. A. Roe. 1994. Sequence of the rec-2 locus of Hemophilus influezae: homologies to comE-ORF3 of Bacillus subtilis and msbA of Escherichia coli. Gene 146:95-100.
9. Dubnau, D. 1993. Genetic exchange and homologous recombination, p. 555-584. In A. L. Sonenshein, J. A. Hoch, and R. Losick (ed.), Bacillus subtilis and other gram-positive bacteria: biochemistry, physiology, and molecular genetics. American Society for Microbiology, Washington, D.C.
10. Dubnau, D., and C. Cirigliano. 1972. Fate of transforming deoxyribonucleic acid after uptake by competent Bacillus subtilis: size and distribution of the integrated donor segments. J. Bacteriol. 111:488-494.
11. Facius, D., and T. F. Meyer. 1993. A novel determinant (comA) essential for natural transformation competence in Neisseria gonorrhoeae. Mol. Microbiol. 10:699-712.
12. Fleischmann, R. D., M. D. Adams, O. White, R. A. Clayton, E. F. Kirkness, A. R. Kerlavage, C. J. Bult, J. F. Tomb, B. A. Dougherty, J. M. Merrick, K. McKenney, G. Sutton, et al. 1995. Whole-genome random sequencing and assembly of Haemophilus influenzae Rd. Science 269:496-512.
13. Freitag, N. E., H. S. Seifert, and M. Koomey. 1995. Characterization of the pilF-pilD pilus-assembly locus of Neisseria gonorrhoeae. Mol. Microbiol. 16: 575-586.
14. Hahn, J., M. Albano, and D. Dubnau. 1987. Isolation and characterization of Tn917lac-generated competence mutants of Bacillus subtilis. J. Bacteriol. 169:3104-3109.
15. Inamine, G., and D. Dubnau. Unpublished data.
16. Inamine, G. S., and D. Dubnau. 1995. ComEA, a Bacillus subtilis integral membrane protein required for genetic transformation, is needed for both DNA binding and transport. J. Bacteriol. 177:3045-3051.
17. Ireton, K., D. Z. Rudner, K. Jaacks-Siranosian, and A. D. Grossman. 1993. Integration of multiple developmental signals in Bacillus subtilis through the SpoOA transcription factor. Genes Dev. 7:283-294.
18. Lunsford, R. D., and A. G. Robel. 1997. comYA, a gene similar to comGA of Bacillus subtilis, is essential for competence-factor-dependent DNA transformation in Streptococcus gordonii. J. Bacteriol. 179:3122-3126.
19. Mohan, S., J. Aghion, N. Guillen, and D. Dubnau. 1989. Molecular cloning and characterization of comC, a late competence gene of Bacillus subtilis. J. Bacteriol. 171:6043-6051.
20. Nunn, D., S. Bergman, and S. Lory. 1990. Products of three accessory genes, pilB, pilC, and pilD, are required for biogenesis of Pseudomonas aeruginosa pili. J. Bacteriol. 172:2911-2919.
21. Nunn, D. N., and S. Lory. 1993. Cleavage, methylation, and localization of the Pseudomonas aeruginosa export proteins XcpT, -U, -V, and -W. J. Bacteriol. 175:4375-4382.
22. Pestova, E. V., and D. A. Morrison. 1997. Streptococcus pneumoniae chromosomal loci specifically induced during competence for genetic transformation, p. 293, abstr. H-49. In Abstracts of the 97th General Meeting of the American Society for Microbiology 1997. American Society for Microbiology, Washington, D.C.
23. Provvedi, R., and D. Dubnau. 1997. Unpublished data.
24. Pugsley, A. P. 1993. The complete general secretory pathway in gram-negative bacteria. Microbiol. Rev. 57:50-108.
25. Sambrook, J., E. F. Fritsch, and T. Maniatis. 1989. Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
26. Schagger, H., and G. von Jagow. 1987. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal. Biochem. 166:368-379.
27. Strom, M. S., D. N. Nunn, and S. Lory. 1993. A single bifunctional enzyme, PilD, catalyzes cleavage and N-methylation of proteins belonging to the type IV pilin family. Proc. Natl. Acad. Sci. USA 90:2404-2408.
28. Tønjum, T., N. E. Freitag, E. Namork, and M. Koomey. 1995. Identification and characterization of pilG, a highly conserved pilus-assembly gene in pathogenic Neisseria. Mol. Microbiol. 16:451-464.
29. Towbin, H., T. Staehelin, and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc. Natl. Acad. Sci. USA 76:4350-4354.