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This paper documents the observation that active scFvs can be produced in L-form cells of E. coli. Apparently, even in the absence of an outer membrane, periplasmic functions are preserved. This allows for the usage of the Gram-negative bacterial 'construction compartment', the periplasm, without the outer membrane barrier
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Wu SC, Ye R, Wu XC, Ng SC, Wong SL: Enhanced secretory production of a single-chain antibody fragment from Bacillus subtilis by coproduction of molecular chaperones. J Bacteriol 1998, 180:2830-2835. This paper documents the finding that the combined overproduction of cytosolic and extra-cytoplasmic chaperones and foldases can improve heterologous protein secretion in B. subtilis.
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