Genomic organization and promoter characterization of the gene encoding the human telomerase reverse transcriptase (hTERT)

Gene

Volumn 232, Issue 1, 1999, Pages 97-106

  Wick, M ^a   Zubov, D ^a   Hagen, G ^a  

^a BAYER AG   (Germany)

Author keywords

Alternative splicing; Cancer; Catalytic subunit; Immortalization; Transcriptional regulation

Indexed keywords

RNA DIRECTED DNA POLYMERASE; TELOMERASE; TRANSCRIPTION FACTOR SP1;

ALTERNATIVE RNA SPLICING; ANIMAL CELL; ARTICLE; CELL AGING; CELL IMMORTALIZATION; CELL STRAIN HL 60; CHO CELL; CONTROLLED STUDY; GENE ISOLATION; HUMAN; HUMAN CELL; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROMOTER REGION; TRANSCRIPTION REGULATION;

ALTERNATIVE SPLICING; BASE SEQUENCE; CELL LINE; EXONS; GENE EXPRESSION REGULATION, ENZYMOLOGIC; HUMANS; INTRONS; MOLECULAR SEQUENCE DATA; PLACENTA; PROMOTER REGIONS (GENETICS); SEQUENCE ANALYSIS, DNA; TELOMERASE; TRANSCRIPTION, GENETIC; TRANSFECTION;

EID: 0033577876     PISSN: 03781119     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0378-1119(99)00108-0     Document Type: Article

References (38)

1. Antequera F., Bird A. Number of CpG islands and genes in human and mouse. Proc. Natl. Acad. Sci. USA. 90:1993;11995-11999.
2. Avilion A.A., Piatyszek M.A., Gupta J., Shay J.W., Bacchetti S., Greider C.W. Human telomerase RNA and telomerase activity in immortal cell lines and tumor tissues. Cancer Res. 56:1996;645-650.
3. Beattie T.L., Zhou W., Robinson M.O., Harrington L. Reconstitution of human telomerase activity in vitro. Curr. Biol. 8:1998;177-180.
4. Blackburn E.H. Structure and function of telomeres. Nature. 350:1991;569-573.
5. Bodnar A.G., Ouellette M., Frolkis M., Holt S.E., Chiu C.-P., Morin G.B., Harley C.B., Shay J.W., Lichtsteiner S., Wright W.E. Extension of life-span by introduction of telomerase into normal human cells. Science. 279:1998;349-352.
6. Boisclair Y.R., Brown A.L., Casola S., Rechler M.M. Three clustered Sp1 sites are required for efficient transcription of the TATA-less promoter of the gene for insulin-like growth factor-binding protein-2 from the rat. J. Biol. Chem. 268:1993;24892-24901.
7. Broccoli D., de Young J.W., Lange T. Telomerase activity in normal and malignant hematopoietic cells. Proc. Natl. Acad. Sci. USA. 92:1995;9082-9086.
8. Collins K., Kobayashi R., Greider C.W. Purification of tetrahymena telomerase and cloning of genes encoding the two protein components of the enzyme. Cell. 81:1995;677-686.
9. Cong Y.S., Wen J., Bacchetti S. The human telomerase catalytic subunit hTERT: organization of the gene and characterization of the promoter. Hum. Mol. Genet. 8:1999;137-142.
10. Counter C.M., Avilion A.A., LeFeuvre C.E., Stewart N.G., Greider C.W., Harley C.B., Bacchetti S. Telomere shortening associated with chromosome instability is arrested in immortal cells which express telomerase activity. EMBO J. 11:1992;1921-1929.
11. Feng J., Funk W.D., Wang S.-S., Weinrich S.L., Avilion A.A., Chiu C.-P., Adams R.R., Chang E., Allsopp R.C., Yu J., Le S., West M.D., Harley C.B., Andrews W.H., Greider C.W., Villeponteau B. The RNA component of human telomerase. Science. 269:1995;1236-1241.
12. Gardiner-Garden M., Frommer M. CpG islands in vertebrate genomes. J. Mol. Biol. 196:1987;261-282.
13. Greider C.W., Blackburn E.H. Identification of a specific telomere terminal transferase activity in tetrahymena extracts. Cell. 43:1985;405-413.
14. Greider C.W., Blackburn E.H. The telomere terminal transferase of tetrahymena is a ribonucleoprotein enzyme with two kinds of primer specificity. Cell. 51:1987;887-898.
15. Härle-Bachor C., Boukamp P. Telomerase activity in the regenerative basal layer of the epidermis in human skin and in immortal and carcinoma-derived skin keratinocytes. Proc. Natl. Acad. Sci. USA. 93:1996;6476-6481.
16. Harley C.B., Futcher A.B., Greider C.W. Telomeres shorten during ageing of human fibroblasts. Nature. 345:1990;458-460.
17. Harley C.B., Vaziri H., Counter C.M., Allsopp R.C. The telomere hypothesis of cellular aging. Exp. Gerontol. 27:1992;375-382.
18. Harrington L., McPhail T., Mar V., Zhou W., Oulton R., Bass M.B., Arruda I., Robinson M.O. A mammalian telomerase-associated protein. Science. 275:1997;973-977.
19. Jones P.A., Taylor S.M., Wilson V. DNA modification, differentiation, and transformation. J. Exp. Zool. 228:1983;287-295.
20. Kilian A., Bowtell D.D.L., Abud H.E., Hime G.R., Venter D.J., Keese P.K., Duncan E.L., Reddel R.R., Jefferson R.A. Isolation of a candidate human telomerase catalytic subunit gene, which reveals complex splicing patterns in different cell types. Hum. Mol. Genet. 6:1997;2011-2019.
21. Kim N.W., Piatyszek M.A., Prowse K.R., Harley C.B., West M.D., Ho P.L., Coviello G.M., Wright W.E., Weinrich S.L., Shay J.W. Specific association of human telomerase activity with immortal cells and cancer. Science. 266:1994;2011-2015.
22. Laird P.W., Jaenisch R. The role of DNA methylation in cancer genetics and epigenetics. Annu. Rev. Genet. 30:1996;441-464.
23. Lingner J., Hughes T.R., Shevchenko A., Mann M., Lundblad V., Cech T.R. Reverse transcriptase motifs in the catalytic subunit of telomerase. Science. 276:1997;561-567.
24. Meyerson M., Counter C.M., Eaton E.N., Ellisen L.W., Steiner P., Caddle S.D., Ziaugra L., Beijersbergen R.L., Davidoff M.J., Liu Q., Bacchetti S., Haber D.A., Weinberg R.A. HEST2, the putative human telomerase catalytic subunit gene, is up-regulated in tumor cells and during immortalization. Cell. 90:1997;785-795.
25. Morin G.B. The human telomere terminal transferase enzyme is a ribonucleoprotein that synthesizes TTAGGG repeats. Cell. 59:1989;521-529.
26. Nakamura T.M., Morin G.B., Chapman K.B., Weinrich S.L., Andrews W.H., Lingner J., Harley C.B., Cech T.R. Telomerase catalytic subunit homologs from fission yeast and human. Science. 277:1997;955-959.
27. Nakayama J., Saito M., Nakamura H., Matsuura A., Ishikawa F. TLP1: a gene encoding a protein component of mammalian telomerase is a novel member of WD repeats family. Cell. 88:1997;875-884.
28. Olovnikov A.M. A theory of marginotomy. The incomplete copying of template margin in enzymic synthesis of polynucleotides and biological significance of the phenomenon. J. Theor. Biol. 41:1973;181-190.
29. Sambrook J., Fritsch E.F., Maniatis T. Molecular Cloning: A Laboratory Manual. 2nd Ed. 1989;Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.
30. Shapiro M.B., Senapathy P. RNA splice junctions of different classes of eukaryotes: sequence statistics and functional implications in gene expression. Nucleic Acids Res. 15:1987;7155-7174.
31. Takakura M., Kyo S., Kanaya T., Hirano H., Takeda J., Yutsudo M., Inoue M. Cloning of the human telomerase catalytic subunit (hTERT) gene promoter and identification of proximal core promoter sequences essential for transcriptional activation in immortalized and cancer cells. Cancer Res. 59:1999;551-557.
32. Ulaner G.A., Hu J.-F., Vu T.H., Giudice L.C., Hoffman A.R. Telomerase activity in human development is regulated by human telomerase reverse transcriptase (hTERT) transcription and by alternate splicing of hTERT transcripts. Cancer Res. 58:1998;4168-4172.
33. Vaziri H., Benchimol S. Reconstitution of telomerase activity in normal human cells leads to elongation of telomeres and extended replicative life span. Curr. Biol. 8:1998;279-282.
34. Wang J., Xie L.Y., Allan S., Beach D., Hannon G.J. Myc activates telomerase. Genes Dev. 12:1998;1769-1774.
35. Weinrich S.L., Pruzan R., Ma L., Ouellette M., Tesmer V.M., Holt S.E., Bodnar A.G., Lichtsteiner S., Kim N.W., Trager J.B., Taylor R.D., Carlos R., Andrews W.H., Wright W.E., Shay J.W., Harley C.B., Morin G.B. Reconstitution of human telomerase with the template RNA component hTR and the catalytic protein subunit hTRT. Nat. Genet. 17:1997;498-502.
36. Wright W.E., Piatyszek M.A., Rainey W.E., Byrd W., Shay J. Telomerase activity in human germline and embryonic tissues and cells. Dev. Genet. 18:1996;173-179.
37. Yasumoto S., Kunimura C., Kikuchi K., Tahara H., Ohji H., Yamamoto H., Ide T., Utakoji T. Telomerase activity in normal human epithelial cells. Oncogene. 13:1996;433-439.
38. Zhao J.-Q., Hoare S.F., McFarlane R., Muir S., Parkinson E.K., Black D.M., Keith W.N. Cloning and characterization of human and mouse telomerase RNA gene promoter sequences. Oncogene. 16:1998;1345-1350.