Enzyme-linked immunosorbent assay for the measurement of JNK activity in cell extracts

Biological Chemistry

Volumn 380, Issue 5, 1999, Pages 569-578

  Tamaskovic, Rastislav ^a   Forrer, Patrik ^a   Jaussi, Rolf ^a  

^a UNIVERSITY OF ZURICH   (Switzerland)

Author keywords

ELISA; Kinase assay; MAPK; Protein immobilisation

Indexed keywords

ANTIBODY; CELL EXTRACT; PROTEIN KINASE;

ACCURACY; ANIMAL CELL; ARTICLE; AUTOMATION; COLORIMETRY; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; IMMOBILIZATION; MOUSE; NONHUMAN; ONCOGENE C JUN; PRIORITY JOURNAL; PROTEIN INTERACTION; PROTEIN PHOSPHORYLATION;

3T3 CELLS; ADENOSINE TRIPHOSPHATE; ANIMALS; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; CELL EXTRACTS; ENZYME-LINKED IMMUNOSORBENT ASSAY; GLUTATHIONE TRANSFERASE; JNK MITOGEN-ACTIVATED PROTEIN KINASES; KINETICS; MICE; MITOGEN-ACTIVATED PROTEIN KINASES; PHOSPHORYLATION; SUBSTRATE SPECIFICITY;

ANIMALIA;

EID: 0032992753     PISSN: 14316730     EISSN: None     Source Type: Journal    
DOI: 10.1515/BC.1999.072     Document Type: Article

References (68)

1. Angeles, T.S., Steffler, C., Bartlett, BA, Hudkins, R.L., Stephens, R.M., Kaplan, D.R., and Dionne, C.A. (1996). Enzyme-linked immunosorbent assay for trkA tyrosine kinase activity. Anal. Biochem. 236, 49-55.
2. Bocco, J.L., Bahr, A., Goetz, J., Hauss, C., Kallunki, T., Kedinger, C., and Chatton, B. (1996). In vivo association of ATFa with JNK/SAP kinase activities. Oncogene 12, 1971-1980.
3. Butler, J.E., Ni, L., Nessler, R., Joshi, K.S., Suter, M., Rosenberg, B., Chang, J., Brown, W.R., and Cantarero, L.A. (1992). The physical and functional behavior of capture antibodies adsorbed on polystyrene. J. Immunol. Methods 150, 77-90.
4. Cavigelli, M., Li, W.W., Lin, A., Su, B., Yoshioka, K., and Karin, M. (1996). The tumor promoter arsenite stimulates AP-1 activity by inhibiting a JNK phosphatase. EMBO J. 15, 6269-6279.
5. Chen, Y.R., Meyer, C.F., and Tan, T.H. (1996). Persistent activation of c-Jun N-terminal kinase 1 (JNK1) in gamma radiation-induced apoptosis. J. Biol. Chem. 271, 631-634.
6. Cheng, C.H., and Hui, S.T. (1996). Immobilized glucose-6-phosphate dehydrogenase as a substrate for solubilized epidermal growth factor receptor tyrosine kinase. A convenient microtiter plate assay system. Appl. Biochem. Biotechnol. 56, 155-167.
7. Choi, K.Y., Satterberg, B., Lyons, D.M., and Elion, E.A. (1994). Ste5 tethers multiple protein kinases in the MAP kinase cascade required for mating in S. cerevisiae. Cell 78, 499-512.
8. Chow, C.W., Rincon, M., Cavanagh, J., Dickens, M., and Davis, R.J. (1997). Nuclear accumulation of NFAT4 opposed by the JNK signal transduction pathway. Science 278, 1638-1641.
9. Cleaveland, J.S., Kiener, P.A., Hammond, D.J., and Schacter, B.Z. (1990). A microtiter-based assay for the detection of protein tyrosine kinase activity. Anal. Biochem. 190, 249-253.
10. Dai, T., Rubie, E., Franklin, C.C., Kraft, A., Gillespie, D.A., Avruch, J., Kyriakis, J.M., and Woodgett, J.R. (1995). Stress-activated protein kinases bind directly to the delta domain of c-Jun in resting cells: implications for repression of c-Jun function. Oncogene 10, 849-855.
11. Derijard, B., Hibi, M., Wu, I.H., Barrett, T., Su, B., Deng, T., Karin, M., and Davis, R.J. (1994). JNK1: a protein kinase stimulated by UV light and Ha-Ras that binds and phosphorylates the c-Jun activation domain. Cell 76, 1025-1037.
12. Fanger, G.R., Johnson, N.L., and Johnson, G.L. (1997). MEK kinases are regulated by EGF and selectively interact with Rac/Cdc42. EMBO J. 16, 4961-4972.
13. Farley, K., Mett, H., McGlynn, E., Murray, B., and Lydon, N.B. (1992). Development of solid-phase enzyme-linked immunosorbent assays for the determination of epidermal growth factor receptor and pp60c-src tyrosine protein kinase activity. Anal. Biochem. 203, 151-157.
14. Forrer, P., and Jaussi, R. (1998). High level expression of soluble heterologous proteins in the cytoplasm of Escherichia coli by fusion to the bacteriophage Lambda head protein D. Gene 224, 45-52.
15. Forrer, P., Tamaskovic, R., and Jaussi, R. (1998). Enzyme-linked immunosorbent assay for measurement of JNK, ERK, and p38 kinase activities. Biol. Chem. 379, 1101-1111.
16. Franz, B., and Stegemann, M. (1991). The kinetics of solid-phase microtiter immunoassays. In: Immunochemistry of Solid-Phase Immunoassay. J.E. Butler, ed. (Boca Raton, USA: CRC Press, Inc.), pp. 277-284.
17. Frey, R.S., and Mulder, K.M. (1997). Involvement of extracellular signal-regulated kinase 2 and stress-activated protein kinase/Jun N-terminal kinase activation by transforming growth factor beta in the negative growth control of breast cancer cells. Cancer Res. 57, 628-633.
18. Fukuda, M., Gotoh, Y., and Nishida, E. (1997). Interaction of MAP kinase with MAP kinase kinase: its possible role in the control of nucleocytoplasmic transport of MAP kinase. EMBO J. 16, 1901-1908.
19. Gille, H., Strahl, T., and Shaw, P.E. (1995). Activation of ternary complex factor Elk-1 by stress-activated protein kinases. Curr. Biol. 5, 1191-1200.
20. Goillot, E., Raingeaud, J., Ranger, A., Tepper, R.I., Davis, R.J., Harlow, E., and Sanchez, I. (1997). Mitogen-activated protein kinase-mediated Fas apoptotic signaling pathway. Proc. Natl. Acad. Sci. USA 94, 3302-3307.
21. Gupta, S., and Davis, R.J. (1994). MAP kinase binds to the NH2-terminal activation domain of c-Myc. FEBS Lett. 353, 281-285.
22. Gupta, S., Campbell, D., Derijard, B., and Davis, R.J. (1995). Transcription factor ATF2 regulation by the JNK signal transduction pathway. Science 267, 389-393.
23. Gupta, S., Barrett, T., Whitmarsh, A.J., Cavanagh, J., Sluss, H.K., Derijard, B., and Davis, R.J. (1996). Selective interaction of JNK protein kinase isoforms with transcription factors. EMBO J. 15, 2760-2770.
24. Han, J., Lee, J.D., Bibbs, L., and Ulevitch, R.J. (1994). A MAP kinase targeted by endotoxin and hyperosmolarity in mammalian cells. Science 265, 808-811.
25. Harrison, B., and Zimmerman, S.B. (1986). T4 polynucleotide kinase: macromolecular crowding increases the efficiency of reaction at DNA termini. Anal. Biochem. 158, 307-315.
26. Hibi, M., Lin, A., Smeal, T., Minden, A., and Karin, M. (1993). Identification of an oncoprotein- and UV-responsive protein kinase that binds and potentiates the c-Jun activation domain. Genes Dev. 7, 2135-2148.
27. Houen, G., and Koch, C. (1997). A non-denaturing enzyme linked immunosorbent assay with protein preadsorbed onto aluminum hydroxide. J. Immunol. Methods 200, 99-105.
28. Hsiao, K.M., Chou, S.Y., Shih, S.J., and Ferrell-JE, J. (1994). Evidence that inactive p42 mitogen-activated protein kinase and inactive Rsk exist as a heterodimer in vivo. Proc. Natl. Acad. Sci. USA 91, 5480-5484.
29. Hu, M.C., Qiu, W.R., Wang, X., Meyer, C.F., and Tan, T.H. (1996). Human HPK1, a novel human hematopoietic progenitor kinase that activates the JNK/SAPK kinase cascade. Genes Dev. 10, 2251-2264.
30. Ichijo, H., Nishida, E., Irie, K., ten Dijke, P., Saitoh, M., Moriguchi, T., Takagi, M., Matsumoto, K., Miyazono, K., and Gotoh, Y. (1997). Induction of apoptosis by ASK1, a mammalian MAP-KKK that activates SAPK/JNK and p38 signaling pathways. Science 275, 90-94.
31. Kallunki, T., Su, B., Tsigelny, I., Sluss, H.K., Derijard, B., Moore, G., Davis, R., and Karin, M. (1994). JNK2 contains a specificity-determining region responsible for efficient c-Jun binding and phosphorylation. Genes Dev. 8, 2996-3007.
32. Kharbanda, S., Saleem, A., Shafman, T., Emoto, Y., Taneja, N., Rubin, E., Weichselbaum, R., Woodgett, J., Avruch, J., Kyriakis, J., et al. (1995). Ionizing radiation stimulates a Grb2-mediated association of the stress-activated protein kinase with phosphatidylinositol 3-kinase. J. Biol. Chem. 270, 18871-18874.
33. Khokhlatchev, A., Xu, S., English, J., Wu, P., Schaefer, E., and Cobb, M.H. (1997). Reconstitution of mitogen-activated protein kinase phosphorylation cascades in bacteria. Efficient synthesis of active protein kinases. J. Biol. Chem. 272, 11057-11062.
34. Kiefer, F., Tibbles, L.A., Anafi, M., Janssen, A., Zanke, B.W., Lassam, N., Pawson, T., Woodgett, J.R., and Iscove, N.N. (1996). HPK1, a hematopoietic protein kinase activating the SAPK/ JNK pathway. EMBO J. 15, 7013-7025.
35. Lazaro, I., Gonzalez, M., Roy, G., Villar, L.M., and Gonzalez, P.P. (1991). Description of an enzyme-linked immunosorbent assay for the detection of protein tyrosine kinase. Anal.Biochem. 192, 257-261.
36. Lehel, C., Daniel, I.S., Brasseur, M., and Strulovici, B. (1997). A chemiluminiscent microtiter plate assay for sensitive detection of protein kinase activity. Anal. Biochem. 244, 340-346.
37. Lim, K., Ho, J.X., Keeling, K., Gilliland, G.L., Ji, X., Ruker, F., and Carter, D.C. (1994). Three-dimensional structure of Schistosoma japonicum glutathione S-transferase fused with a six-amino acid conserved neutralizing epitope of gp41 from HIV. Protein Sci. 3, 2233-2244.
38. Livingstone, C., Patel, G., and Jones, N. (1995). ATP-2 contains a phosphorylation-dependent transcriptional activation domain. EMBO J. 14, 1785-1797.
39. Maehara, S., Kawashita, E., Himeno, Y., Ishibe, T., Toki, N., Sumi, H., Tanaka, Y., and Sasaki, K. (1985). Immunochemical determination of the serum protein reacting with antibody against human urinary trypsin inhibitor by single radial immunodiffusion: use of polyethylene glycol. J. Immunol. Methods 80, 117-123.
40. Mao, C., Ray, G.D., Tavitian, A., and Moreau, G.F. (1996). Differential phosphorylations of Spi-B and Spi-1 transcription factors. Oncogene 12, 863-873.
41. Marcus, S., Polverino, A., Barr, M., and Wigler, M. (1994). Complexes between STE5 and components of the pheromone-responsive mitogen-activated protein kinase module. Proc. Natl. Acad. Sci. USA 91, 7762-7766.
42. Marti, A., Luo, Z., Cunningham, C., Ohta, Y., Hartwig, J., Stossel, T.P., Kyriakis, J.M., and Avruch, J. (1997). Actin-binding protein-280 binds the stress-activated protein kinase (SAPK) activator SEK-1 and is required for tumor necrosis factor-alpha activation of SAPK in melanoma cells. J. Biol. Chem. 272, 2620-2628.
43. Nishina, H., Fischer, K.D., Radvanyi, L., Shahinian, A., Hakem, R., Rubie, E.A., Bernstein, A., Mak, T.W., Woodgett, J.R., and Penninger, J.M. (1997). Stress-signalling kinase Sek1 protects thymocytes from apoptosis mediated by CD95 and CD3. Nature 385, 350-353.
44. Nygren, H., and Stenberg, M. (1985). Kineticsof antibody-binding to surface-immobilized antigen: influence of mass transport on the enzyme-linked immunosorbent assay (ELISA). J. Colloid Interface Sci. 107, 560-566.
45. Nygren, H., and Stenberg, M. (1991). Rate limitation of the antigen-antibody reactions: theoretical and practical aspects. In: Immunochemistry of Solid-Phase Immunoassay. J.E. Butler, ed. (Boca Raton, USA: CRC Press, Inc.), pp. 285-291.
46. Pombo, C.M., Bonventre, J.V., Avruch, J., Woodgett, J.R., Kyriakis, J.M., and Force, T. (1994). The stress-activated protein kinases are major c-Jun amino-terminal kinases activated by ischemia and reperfusion. J. Biol. Chem. 269, 26546-26551.
47. Posas, F., and Saito, H. (1997). Osmotic activation of the HOG MARK pathway via Ste11p MAPKKK: scaffold role of Pbs2p MAPKK. Science 276, 1702-1705.
48. Raitano, A.B., Halpern, J.R., Hambuch, T.M., and Sawyers, C.L. (1995). The Bcr-Abl leukemia oncogene activates Jun kinase and requires Jun for transformation. Proc. Natl. Acad. Sci. USA 92, 11746-11750.
49. Read, M.A., Whitley, M.Z., Gupta, S., Pierce, J.W., Best, J., Davis, R.J., and Collins, T. (1997). Tumor necrosis factor alpha-induced E-selectin expression is activated by the nuclear factor-kappaB and c-JUN N-terminal kinase/p38 mitogen-activated protein kinase pathways. J. Biol. Chem. 272, 2753-2761.
50. Riesgo Escovar, J.R., Jenni, M., Fritz, A., and Hafen, E. (1996).The Drosophila Jun-N-terminal kinase is required for cell morphogenesis but not for DJun-dependent cell fate specification in the eye. Genes Dev. 10, 2759-2768.
51. Robinson, M.J., Harkins, P.C., Zhang, J., Baer, R., Haycock, J.W., Cobb, M.H., and Goldsmith, E.J. (1996). Mutation of position 52 in ERK2 creates a nonproductive binding mode for adenosine 5′-triphosphate. Biochemistry 35, 5641-5646.
52. Sanchez, I., Hughes, R.T., Mayer, B.J., Yee, K., Woodgett, J.R., Avruch, J., Kyriakis, J.M., and Zon, L.I. (1994). Role of SAPK/ ERK kinase-1 in the stress-activated pathway regulating transcription factor c-Jun. Nature 372, 794-798.
53. Schwab, C., and Bosshard, H.R. (1992). Caveats for the use of surface-adsorbed protein antigen to test the specificity of antibodies. J. Immunol. Methods 147, 125-134.
54. Sluss, H.K., Han, Z., Barrett, T., Davis, R.J., and Ip, Y.T. (1996). A JNK signal transduction pathway that mediates morphogenesis and an immune response in Drosophila. Genes Dev. 10, 2745-2758.
55. Su, B., Jacinto, E., Hibi, M., Kallunki, T., Karin, M., and Ben, N.Y. (1994). JNK is involved in signal integration during costimulation of T lymphocytes. Cell 77, 727-736.
56. Teramoto, H., Coso, O.A., Miyata, H., Igishi, T., Miki, T., and Gutkind, J.S. (1996). Signaling from the small GTP-binding proteins Rad and Cdc42 to the c-Jun N-terminal kinase/stress-activated protein kinase pathway. A role for mixed lineage kinase 3/protein-tyrosine kinase 1, a novel member of the mixed lineage kinase family. J. Biol. Chem. 271, 27225-27228.
57. Verheij, M., Bose, R., Lin, X.H., Yao, B., Jarvis, W.D., Grant, S., Birrer, M.J., Szabo, E., Zon, L.I., Kyriakis, J.M., Haimovitz Friedman, A., Fuks, Z., and Kolesnick, R.N. (1996). Requirement for ceramide-initiated SAPK/JNK signalling in stress-induced apoptosis. Nature 380, 75-79.
58. Waskiewicz, A.J., Flynn, A., Proud, C.G., and Cooper, J.A. (1997). Mitogen-activated protein kinases activate the serine/threonine kinases Mnk1 and Mnk2. EMBO J. 16, 1909-1920.
59. Whitmarsh, A.J., and Davis, R.J. (1996). Transcription factor AP-1 regulation by mitogen-activated protein kinase signal transduction pathways. J. Mol. Med. 74, 589-607.
60. Whitmarsh, A.J., Cavanagh, L., Tournier, C., Yasuda, L., and Davis, R.J. (1998). Mammalian scaffold complex that selectively mediates MAP kinase activation. Science 287, 1671-1674.
61. Xia, Z., Dickens, M., Raingeaud, J., Davis, R.J., and Greenberg, M.E. (1995). Opposing effects of ERK and JNK-p38 MAP kinases on apoptosis. Science 270, 1326-1331.
62. Xu, X., Heidenreich, O., Kitajima, I., McGuire, K., Li, Q., Su, B., and Nerenberg, M. (1996). Constitutively activated JNK is associated with HTLV-1 mediated tumorigenesis. Oncogens 13, 135-142.
63. Van, M., Dai, T., Deak, J.C., Kyriakis, J.M., Zon, L.I., Woodgett, J.R., and Templeton, D.J. (1994). Activation of stress-activated protein kinase by MEKK1 phosphorylation of its activator SEK1. Nature 372, 798-800.
64. Yang, D.D., Kuan, C.Y., Whitmarsh, A.J., Rincon, M., Zheng, T.S., Davis, R.J., Rakic, P., and Flavell, R.A. (1997). Absence of excitotoxicity-induced apoptosis in the hippocampus of mice lacking the Jnk3 gene. Nature 389, 865-870.
65. Yang, S.H., Whitmarsh, A.J., Davis, R.J., and Sharrocks, A.D. (1998a). Differential targeting of MAP kinase to the ETS-domain transcription factor Elk-1. EMBO J. 17, 1740-1749.
66. Yang, S.H., Yates, P.R., Whitmarsh, A.J., Davis, R.J., and Sharrocks, A.D. (1998b). The Elk-1 ETS-domain transcription factor contains a mitogen-activated protein kinase targeting motif. Mol. Cell Biol. 18, 710-720.
67. Zanke, B.W., Boudreau, K., Rubie, E., Winnett, E., Tibbles, L.A., Zon, L., Kyriakis, J., Liu, F.F., and Woodgett, J.R. (1996a). The stress-activated protein kinase pathway mediates cell death following injury induced by cis-platinum, UV irradiation or heat. Curr. Biol. 6, 606-613.
68. Zanke, B.W., Rubie, E.A., Winnett, E., Chan, J., Randall, S., Parsons, M., Boudreau, K., McInnis, M., Van, M., Templeton, D.J., and Woodgett, J.R. (1996b). Mammalian mitogen-activated protein kinase pathways are regulated through formation of specific kinase-activator complexes. J. Biol. Chem. 271, 29876-29881.