A mammalian scaffold complex that selectively mediates MAP kinase activation

Science

Volumn 281, Issue 5383, 1998, Pages 1671-1674

  Whitmarsh, Alan J ^a   Cavanagh, Julie ^a   Tournier, Cathy ^a   Yasuda, Jun ^a   Davis, Roger J ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

MITOGEN ACTIVATED PROTEIN KINASE; TRANSCRIPTION FACTOR;

ARTICLE; ENZYME ACTIVATION; GENE OVEREXPRESSION; IMMUNOBLOTTING; MAMMAL CELL; NONHUMAN; ONCOGENE C JUN; PHOSPHORYLATION; PRIORITY JOURNAL; SACCHAROMYCES CEREVISIAE; SIGNAL TRANSDUCTION; STRESS;

ANIMALS; CA(2+)-CALMODULIN DEPENDENT PROTEIN KINASE; CARRIER PROTEINS; CELL LINE; CERCOPITHECUS AETHIOPS; COS CELLS; ENZYME ACTIVATION; INTERLEUKIN-1; JNK MITOGEN-ACTIVATED PROTEIN KINASES; MAP KINASE KINASE 7; MAP KINASE KINASE KINASES; MITOGEN-ACTIVATED PROTEIN KINASE KINASES; MITOGEN-ACTIVATED PROTEIN KINASES; MOLECULAR SEQUENCE DATA; PROTEIN KINASES; PROTEIN-SERINE-THREONINE KINASES; RECOMBINANT FUSION PROTEINS; SIGNAL TRANSDUCTION;

EID: 0032508714     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.281.5383.1671     Document Type: Article

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31. 2; 60 min). The cells were collected at 48 hours and solubilized in lysis buffer [20 mM tris-HCl (pH 7.4), 137 mM NaCl, 2 mM EDTA, 25 mM β-glycerophosphate, 2 mM pyrophosphate, 1 mM sodium orthovanadate, 1 mM phenylmethylsulfonyl fluoride, leupeptin (10 μg/ml), 10% (v/v) glycerol, 1% Triton X-100]. GST fusion proteins were isolated by incubation with glutathione-agarose (Pharmacia-LKB) beads (20 μl) for 3 hours at 4°C. Epitope-tagged proteins were immunoprecipitated by incubation for 3 hours at 4°C with the monoclonal antibodies M2-Flag (IBI-Kodak), hemagglutinin (HA) (Boehringer-Mannheim), or T7-Tag (Novagen Inc.) bound to protein G-Sepharose (Pharmacia-LKB). HPK1 was immunoprecipitated with a rabbit polyclonal antibody to HPK1. Immunoprecipitated proteins were examined by SDS-polyacrylamide gel-electrophoresis and detected by immunoblot analysis. Protein kinase activity was measured by the in-gel method with substrate polymerized in the gel (0.25 mg/ml) [B. Dérijard et al., Cell 76, 1025 (1994)].
32. 2-terminus using the vector pCDNA3.
33. Bacterial expression of GST-cJun (1 to 79), GST-JNK1, and GST-JIP-1 (127 to 282) have been described (4). Expression plasmids for GST-JIP-1 (1 to 127) and GST-JIP-1 (283 to 660) were constructed by subcloning polymerase chain reaction fragments of JIP-1 into pGEX-4T-1 (Pharmacia-LKB). Bacterial expression plasmids for epitope-tagged MKK7 and MLK3 were constructed by inserting Flag-MKK7 and Flag-MLK3 (encoding amino acids 1 to 204) into pRSETA (Invitrogen).
34. We thank our colleagues for providing essential reagents, T. Barrett for DNA sequence analysis, and K. Gemme for secretarial assistance. Supported by a grant from the National Cancer Institute. R.J.D. is an Investigator of the Howard Hughes Medical Institute.