Analysis of assembly of synthetic antibody fragments: Expression of functional scFv with predefined specificity

BioTechniques

Volumn 23, Issue 3, 1997, Pages 500-503

  Kobayashi, Norihiro ^a   Söderlind, Eskil ^a   Borrebaeck, Carl A K ^a  

^a LUND UNIVERSITY   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

ANTIBODY; OLIGONUCLEOTIDE;

ARTICLE; GENE STRUCTURE; PHAGE DISPLAY; POLYMERASE CHAIN REACTION;

EID: 0343907773     PISSN: 07366205     EISSN: None     Source Type: Journal    
DOI: 10.2144/97233st09     Document Type: Article

References (9)

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3. Griffiths, A.D., S.C. Williams, O. Hartley, I.M. Tomlinson, P. Waterhouse, W.L. Crosby, R.E. Konterman, P.T. Jones et al. 1994. Isolation of high affinity human antibodies directly from large synthetic repertoires. EMBO J. 13:3245-3260.
4. Hayashi, N., M. Welschof, M. Zewe, M. Braunagel, S. Dübel, F. Breitling and M. Little. 1994. Simultaneous mutagenesis of antibody CDR regions by overlap extension and PCR. BioTechniques 17:310-315.
5. Kütemeier, G., W. Höhne, T. Werner, R. Schuh and R. Mocikat. 1994. Assembly of humanized antibody genes from synthetic oligonucleotides using a single-round PCR. BioTechniques 17:242-246.
6. Marks, J.D. 1995. Human monoclonal antibodies from V-gene repertoires expressed on bacteriophage, p. 53-88. In C.A.K. Borrebaeck (Ed.), Antibody Engineering, 2nd ed. Oxford University Press, Oxford.
7. Söderlind, E., A.-C. Simonsson Lagerkvist, M. Dueñas, A.-C. Malmborg, L. Danielsson and C.A.K. Borrebaeck. 1993. Chaperonin assisted phage display of antibody fragments on filamentous bacteriophages. Bio/Technology 11:503-507.
8. Söderlind, E., M. Vergeles and C.A.K. Borrebaeck. 1995. Domain libraries: synthetic diversity for de novo design of antibody V-regions. Gene 160:269-272.
9. I.M. Tomlinson, S.C. Williams, O. Ignatovich, J. Corbett and G. Winter. 1995. V BASE Sequence Directory, MRC Centre for Protein Engineering, Cambridge, UK. World Wide Web location: http://www.mrc-cpe.cam. ac.uk/imt-doc/vbase-home-page.html