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Protein engineering of antibody binding sites: Recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli
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Huston JS, Levinson D, Mudgett-Hunter M, Tai MS, Novotnyu J, Margolies MN, Ridge RJ, Bruccoleri RE, Haber E, Crea R. Protein engineering of antibody binding sites: recovery of specific activity in an anti-digoxin single-chain Fv analogue produced in Escherichia coli. Proc Natl Acad Sci USA. 85:1988;5879-5883.
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The cloning of hybridoma V regions for their ectopic expression in intracellular and intercellular immunization
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of special interest. Borrebaeck C.A.K. edn 2 Oxford University Press, Oxford, A nice summary of the cloning of hybridoma V regions and their use in creating novel molecules. The whole book is destined to become a classic reference for researchers in Ab engineering
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Bradbury A, Ruberti F, Werge T, Amati V, Di Luzio A, Gonfloni S, Hoogenboom H, Picciioli P, Biocca S, Cattaneo A. The cloning of hybridoma V regions for their ectopic expression in intracellular and intercellular immunization. of special interest Borrebaeck CAK. edn 2 Antibody Engineering: a Practical Guide. 1995;295-353 Oxford University Press, Oxford, A nice summary of the cloning of hybridoma V regions and their use in creating novel molecules. The whole book is destined to become a classic reference for researchers in Ab engineering.
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Gilliland LK, Norris NA, Marquardt H, Tsu TT, Hayden MS, Neubauer MG, Yelton DE, Mittler RS, Ledbetter JA. Rapid and reliable cloning of antibody V regions and generation of recombinant single chain antibody fragments. Tissue Antigens. 47:1996;1-20.
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Marks JD. Human monoclonal antibodies from V-gene repertoires expressed on bacteriophage. of special interest Borrebaeck CAK. edn 2 Antibody Engineering: a Practical Guide. 1995;53-69 Oxford University Press, Oxford, Another chapter from this excellent reference, including basic methods and protocols for generating and manipulating phage display libraries.
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Marks, J.D.1
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Glaser S, Kristensson K, Chilton T, Huse W. Engineering the antibody combining site by codon-based mutagenesis in a filamentous phage display system. Borrebaeck CAK. edn 2 Antibody Engineering: a Practical Guide. 1995;117-121 Oxford University Press, Oxford.
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0029008062
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Rapid selection of cell subpopulation specific human monoclonal antibodies from a synthetic phage antibody library
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of outstanding interest. Novel application of subtractive/sorted selection techniques for isolating new human anti-human binding specificities from heterogeneous Ab mixtures.
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De Kruif J, Terstappen L, Boel E, Logtenberg T. Rapid selection of cell subpopulation specific human monoclonal antibodies from a synthetic phage antibody library. of outstanding interest Proc Natl Acad Sci USA. 92:1995;3938-3942 Novel application of subtractive/sorted selection techniques for isolating new human anti-human binding specificities from heterogeneous Ab mixtures.
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0029839470
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A novel strategy for generating monoclonal antibodies from single, isolated lymphocytes producing antibodies of defined specificities
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of outstanding interest. A recent paper which combines the power of Ab selection techniques with PCR to achieve single cell cloning of desired Ab cDNAs.
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Babcook JS, Leslie KB, Olsen OA, Salmon RA, Schrader JW. A novel strategy for generating monoclonal antibodies from single, isolated lymphocytes producing antibodies of defined specificities. of outstanding interest Proc Natl Acad Sci USA. 93:1996;7843-7848 A recent paper which combines the power of Ab selection techniques with PCR to achieve single cell cloning of desired Ab cDNAs.
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Surface expression of CD28 single chain Fv for costimulation by tumor cells
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of special interest. An extension of the intracellular expression of scFvs leading to cell surface expression thereby creating artificial adhesion receptors from scFv binding specificities.
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Winberg G, Grosmaire LS, Klussman K, Hayden MS, Fell HP, Ledbetter JA, Mittler RS. Surface expression of CD28 single chain Fv for costimulation by tumor cells. of special interest Immunol Rev. 153:1996;6-14 An extension of the intracellular expression of scFvs leading to cell surface expression thereby creating artificial adhesion receptors from scFv binding specificities.
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Winberg, G.1
Grosmaire, L.S.2
Klussman, K.3
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Ledbetter, J.A.6
Mittler, R.S.7
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19
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0029072102
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Activity of a single-chain immunotoxin that selectively kills lymphoma and other B-lineage cells expressing the CD40 antigen
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Francisco JA, Gilliland LK, Stebbins MR, Norris NA, Ledbetter JA, Siegall CB. Activity of a single-chain immunotoxin that selectively kills lymphoma and other B-lineage cells expressing the CD40 antigen. Cancer Res. 55:1995;3099-3104.
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Reiter Y, Brinkmann U, Lee B, Pastan I. Engineering antibody Fv fragments for cancer detection and therapy: disulfide-stabilized Fv fragments. Nat Biotechnol. 14:1996;1239-1245.
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Reiter Y, Pastan I. Antibody engineering of recombinant Fv immunotoxins for improved targeting of cancer: disulfide-stabilized Fv immunotoxins. Clin Cancer Res. 2:1996;245-252.
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24
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Single chain mono- And bispecific antibody derivatives with novel biological properties and anti-tumor activity from a COS cell transient expression system
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Hayden MS, Linsley PS, Gayle MG, Bajorath J, Brady WA, Norris NA, Fell HP, Ledbetter JA, Gilliland LK. Single chain mono- and bispecific antibody derivatives with novel biological properties and anti-tumor activity from a COS cell transient expression system. Ther Immunol. 1:1994;3-15.
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Development and characterization of anti-renal cell carcinoma x antichelate bispecific monoclonal antibodies for two-phase targeting of renal cell carcinoma
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Kranenborg MHGC, Boerman OC, Oosterwijk-Wakka JC, De Weijert MCA, Corstens FHM, Oosterwijk E. Development and characterization of anti-renal cell carcinoma x antichelate bispecific monoclonal antibodies for two-phase targeting of renal cell carcinoma. Cancer Res. 55:1995;5864-5867.
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0028842806
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G-CSF-stimulated PMN in immunotherapy of breast cancer with a bispecific antibody to FcgammaRI and to HER-2/neu (MDX-210)
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Repp R, Valerius T, Wieland G, Becker W, Steininger H, Deo Y, Helm G, Gramatzki M, Van De Winkel JGJ, Lang N, Kalden JR. G-CSF-stimulated PMN in immunotherapy of breast cancer with a bispecific antibody to FcgammaRI and to HER-2/neu (MDX-210). J Hematotherapy. 4:1995;415-421.
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Repp, R.1
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Alternative triggering molecules and single chain bispecific antibodies
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Segal DM, Sconocchia G, Titus JA, Jost CR, Kurucz I. Alternative triggering molecules and single chain bispecific antibodies. J Hematotherapy. 4:1995;377-382.
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Segal, D.M.1
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Kurucz, I.5
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30
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0028822836
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Phase I trial of 2B1, a bispecific monoclonal antibody targeting c-erbB-2 and FcgammaRIII
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Weiner LM, Clark JI, Davey M, Li WS, Garcia De Palazzo I, Ring DB, Alpaugh RK. Phase I trial of 2B1, a bispecific monoclonal antibody targeting c-erbB-2 and FcgammaRIII. Cancer Res. 55:1995;4586-4593.
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Weiner, L.M.1
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Ring, D.B.6
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0029077481
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2 made by protein folding and bonded through C-terminal cysteinyl peptides
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2 made by protein folding and bonded through C-terminal cysteinyl peptides. Protein Eng. 8:1995;301-314.
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McCartney, J.E.1
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Stafford W.F. III7
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Costimulation by CD28 sFv expressed on the tumor cell surface or as a soluble bispecific molecule targeted to the L6 carcinoma antigen
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Hayden MS, Grosmaire LS, Norris NA, Gilliland LK, Winberg G, Tritschler D, Tsu TT, Linsley PS, Mittler RS, Senter PD, et al. Costimulation by CD28 sFv expressed on the tumor cell surface or as a soluble bispecific molecule targeted to the L6 carcinoma antigen. Tissue Antigens. 48:1996;242-254.
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1 lymphoma: increased efficacy with multiple injections and CD28-induced costimulation. Blood. 87:1996;4390-4398.
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36
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0028823836
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Targeted eradication of ovarian cancer mediated by intracellular expression of anti-erbB-2 single-chain antibody
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of special interest. One of the first definitive reports of intracellular Ab fragments for potential use in tumor therapy.
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Deshane J, Cabrera G, Grim JE, Siegal GP, Pike J, Alvarez RD, Curiel DT. Targeted eradication of ovarian cancer mediated by intracellular expression of anti-erbB-2 single-chain antibody. of special interest Gynecol Oncol. 59:1995;8-14 One of the first definitive reports of intracellular Ab fragments for potential use in tumor therapy.
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Gynecol Oncol
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Deshane, J.1
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Design, intracellular expression, and activity of a human anti-human immunodeficiency virus type 1 gp120 single-chain antibody
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Marasco WA, Haseltine WA, Chen S-Y. Design, intracellular expression, and activity of a human anti-human immunodeficiency virus type 1 gp120 single-chain antibody. Proc Natl Acad Sci USA. 90:1993;7889-7893.
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Duan L, Bagasra O, Laughlin MA, Oakes JW, Pomerantz RJ. Potent inhibition of human immunodeficiency virus type 1 replication by an intracellular anti-Rev single chain antibody. Proc Natl Acad Sci USA. 91:1994;5075-5079.
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Werge TM, Baldari CT, Telford JL. Intracellular single chain Fv antibody inhibits Ras activity in T-cell antigen receptor stimulated Jurkat cells. FEBS Lett. 351:1994;393-396.
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Werge, T.M.1
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Telford, J.L.3
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0028839655
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Targeted tumor killing via an intracellular antibody against erbB-2
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of special interest. This paper describes the construction and adenovirus-mediated expression of intracellular Abs against erbB-2 in tumor cells. It is a companion paper to [36].
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Deshane J, Siegal GP, Alvarez RD, Wang MH, Feng M, Caberra G, Liu T, Kay M, Curiel DT. Targeted tumor killing via an intracellular antibody against erbB-2. of special interest J Clin Invest. 96:1995;2980-2989 This paper describes the construction and adenovirus-mediated expression of intracellular Abs against erbB-2 in tumor cells. It is a companion paper to [36].
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J Clin Invest
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Deshane, J.1
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Liu, T.7
Kay, M.8
Curiel, D.T.9
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0028997906
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Intracellular immunization: Antibody targeting to subcellular compartments
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of special interest. A nice review of subcellular targeting approaches and the sequence motifs necessary to achieve localization of expressed Ab fragments.
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Biocca S, Cataneo A. Intracellular immunization: antibody targeting to subcellular compartments. of special interest Trends Cell Biol. 5:1995;248-252 A nice review of subcellular targeting approaches and the sequence motifs necessary to achieve localization of expressed Ab fragments.
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0028877140
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Production of monoclonal antibodies in COS and CHO cells
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of special interest. An interesting review of selected mammalian expression techniques used for Ab production.
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Trill JJ, Shatzman AR, Ganguly S. Production of monoclonal antibodies in COS and CHO cells. of special interest Curr Opin Biotechnol. 6:1995;553-560 An interesting review of selected mammalian expression techniques used for Ab production.
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