The L3 loop: A structural motif determining specific interactions between SMAD proteins and TGF-β receptors

EMBO Journal

Volumn 17, Issue 4, 1998, Pages 996-1005

  Lo, Roger S ^a   Chen, Ye Guang ^a   Shi, Yigong ^a   Pavletich, Nikola P ^a   Massagué, Joan ^a  

^a HOWARD HUGHES MEDICAL INSTITUTE   (United States)

Author keywords

L3 loop; Receptor interactions; SMAD proteins; Transforming growth factor

Indexed keywords

BONE MORPHOGENETIC PROTEIN; GROWTH FACTOR RECEPTOR; TRANSFORMING GROWTH FACTOR BETA;

ANIMAL CELL; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; HUMAN; NONHUMAN; PRIORITY JOURNAL; PROTEIN SECONDARY STRUCTURE; SIGNAL TRANSDUCTION; TRANSCRIPTION REGULATION;

AMINO ACID SEQUENCE; BASIC HELIX-LOOP-HELIX LEUCINE ZIPPER TRANSCRIPTION FACTORS; DNA-BINDING PROTEINS; HUMANS; MODELS, MOLECULAR; MOLECULAR SEQUENCE DATA; PHOSPHORYLATION; PROTEIN STRUCTURE, TERTIARY; RECEPTORS, TRANSFORMING GROWTH FACTOR BETA; REPRESSOR PROTEINS; SIGNAL TRANSDUCTION; SMAD2 PROTEIN; STRUCTURE-ACTIVITY RELATIONSHIP; TRANS-ACTIVATORS;

ANIMALIA;

EID: 0032481351     PISSN: 02614189     EISSN: None     Source Type: Journal    
DOI: 10.1093/emboj/17.4.996     Document Type: Article

References (42)

1. Baker, J. and Harland, R.M. (1996) A novel mesoderm inducer, Madr-2, functions in the activin signal transduction pathway. Genes Dev., 10, 1880-1889.
2. Boyd, F.T. and Massagué J. (1989) Growth inhibitory response to transforming growth factor- linked to expression of a 53 kDa cell surface TGF-β receptor. J. Biol. Chem., 264, 2272-2278.
3. Chen, X., Weisberg, E., Fridmacher, V., Watanabe, M., Naco, G. and Whitman, M. (1997) Smad4 and FAST-1 in the assembly of activin-responsive factor. Nature, 389, 85-89.
4. Chen, Y., Bhushan, A. and Vale, W. (1997) Smad8 mediates the signaling of the receptor serine kinase. Proc. Natl Acad. Sci. USA, 94, 12938-12943.
5. Chen, Y.G., Liu, F. and Massagué, J. (1997) Mechanism of TGFβ receptor inhibition by FKBP12. EMBO J., 16, 3866-3876.
6. Derynck, R. and Zhang, Y. (1996) Intracellular signaling: the Mad way to do it. Curr. Biol., 6, 1226-1229.
7. Eppert, K. et al. (1996) MADR2 maps to 18q21 and encodes a TGFβregulated MAD-related protein that is functionally mutated in colorectal carcinoma. Cell, 86, 543-552.
8. Graff, J.M., Bansal, A. and Melton, D.A. (1996) Xenopus Mad proteins transduce distinct subsets of signals for the TGFβ superfamily. Cell, 85, 479-487.
9. Hahn, S.A. et al. (1996) DPC4, a candidate tumor suppressor gene at human chromosome 18q21.1. Science, 271, 350-353.
10. Hata, A., Lo, R.S., Wotton, D., Lagna, G. and Massagué, J. (1997) Mutations increasing autoinhibition inactivate the tumour suppressors Smad2 and Smad4. Nature, 388, 82-86.
11. Hata, A., Lagna, G., Massagué, J. and Hemmati-Brivanlou, A. (1998) Smad6 inhibits BMP/Smad1 signaling by specifically competing with the Smad4 tumor suppressor. Genes Dev., 12, 186-197.
12. Hayashi, H. et al. (1997) The MAD-related protein Smad7 associates with the TGFβ receptor and functions as an antagonist of TGFβ signaling. Cell, 89, 1165-1173.
13. Hill, C.S. and Treisman, R. (1995) Transcriptional regulation by extracellular signals: mechanisms and specificity. Cell, 80, 199-211.
14. Hoodless, P.A., Haerry, T., Abdollah, S., Stapleton, M., O'Connor, M.B., Attisano, L. and Wrana, J.L. (1996) MAOR1, a MAD-related protein that functions in BMP2 signalling pathways. Cell 85, 489-500.
15. Imamura, T., Takase, M., Nishihara, A., Oeda, E., Hanai, J., Kawabata, M. and Miyazono, K. (1997) Smad6 inhibits signalling by the TGF-beta superfamily. Nature, 389, 622-626.
16. Kim, J., Johnson, K., Chen, H.J., Carroll, S. and Laughon, A. (1997) Drosophila Mad binds to DNA and directly mediates activation of vestigial by Decapentaplegic. Nature, 388, 304-308.
17. Kretzschmar, M., Doody, J. and MassaguéJ. (1997a) Opposing BMP and EGF signalling pathway converge on the TGFβ family mediator Smad1. Nature, 389, 618-622.
18. Kretzschmar, M., Liu, F., Hata, A., Doody, J. and Massagué, J. (1997b) The TGF-β mediator Smad1 is directly phosphorylated and functionally activated by the BMP receptor kinase. Genes Dev., 11, 984-995.
19. Lagna, G., Hata, A., Hemmati-Brivanlou, A. and Massagué, J. (1996) Partnership between DPC4 and SMAD proteins in TGFβ signalling pathways. Nature, 383, 832-836.
20. Liu, F., Hata, A., Bakcer, J., Doody, J., Cárcamo, J., Harland, R. and MassaguéJ. (1996) A human Mad protein acting as a BMP-regulated transcriptional activator. Nature, 381, 620-623.
21. Liu, F., Pouponnot, C. and Massagué, J. (1997) Dual role of the Samd4/ DPC4 tumor suppressor in TGFβ-inducible transcriptional complexes. Genes Dev., 11, 3157-3167.
22. Macias-Silva, M., Abdollah, S., Hoodless, P.A., Pirone, R., Attisano, L. and Wrana, J.L. (1996) MADR2 is a substrate of the TGFβ receptor and phosphorylation is required for nuclear accumulation and signaling. Cell, 87, 1215-1224.
23. Massagué, J. (1987) Identification of receptors of type βtransforming growth factor. Methods Enzymol, 146m 174-195.
24. MassaguéJ. (1996) TGFβ signaling: receptors, transducers and Mad proteins. Cell, 85, 947-950.
25. Massagué, J., Hata, A. and Liu, F. (1997) TGFβ signaling through the Smad pathway. Trends Cell Biol., 7, 187-192.
26. Nakao, A., Röijer, E., Imamura, T., Souchelnytskyi, S., Stenman, G., Heldin, C.-H. and ten Dijke, P. (1997a) Identification of Smad2, a human Mad-related protein in the transforming growth factor-β signaling pathway. J. Biol. Chem., 272, 2896-2900.
27. Nakao, A. et al. (1997b) Identification of Smad7, a TGF beta-inducible antagonist of TGF-beta signalling. Nature, 389, 631-635.
28. Newfeld, S.J., Mehra, A., Singer, M.A., Wrana, J.L., Attisano, L. and Gelbart, W.M. (1997) Mothers against dpp participates in a DPP/TGF-β responsive serine/threonine kinase signal transduction cascade. Development, 124, 3167-3176.
29. Pawson, T. and Scott, J.D. (1997) Signaling through scaffold, anchoring and adaptor proteins. Science, 278, 2075-2080.
30. Savage, C., Das, P., Finelli, A., Townsend, S.R., Sun, C.-Y., Baird, S.E. and Padgett, R.W. (1996) Caenorhabditis elegans genes sma-2, sma-3 and sma-4 define a conserved family of transforming growth factor β pathway components. Proc. Natl Acad. Sci. USA, 93, 790-794.
31. Sekeisky, J.J., Newfeld, S.J., Raftery, L.A., Chartoff, E.H. and Gelbart, W.M. (1995) Genetic characterization and cloning of Mothers against dpp, a gene required for decapentaplegic function in Drosophila melanogaster. Genetics, 139, 1347-1358.
32. Shi, Y., Hata, A., Lo, R.S., Massagué, J. and Pavletich, N.P. (1997) A structural basis for mutational inactivation of the tumour suppressor Smad4. Nature, 388, 87-93.
33. Thomsen, G. (1996) Xenopus mothers against decapentaplegic is an embryonic ventralizing agent that acts downstream of the BMP-2/4 receptor. Development, 122, 2359-2366.
34. Topper, J.N. et al. (1997) Vascular MADs: two novel MAD-related genes selectively inducible by flow in human vascular endothelium. Proc. Natl Acad. Sci. USA, 94, 9314-9319.
35. Tsuneizumi, K., Nakayama, T., Kamoshida, Y., Kornberg, T., Christian, J.L. and Tabata, T. (1997) Daughters against dpp modulates dpp organizing activity in Drosophila wing development. Nature, 389, 627-631.
36. Watanabe, T.K., Suzuki, M., Omori, Y., Hishigaki, H., Horie, M., Kanemoto, N., Fujiwara, T. Nakamura, Y. and Takahashi, E. (1997) Cloning and characterization of a novel member of the human Mad gene family (MADH6). Genomics, 42, 446-451.
37. Wiersdorff, V., Lecuit, T., Cohen, S.M. and Mlodzik, M. (1996) Mad acts downstream of Dpp receptors, revealing differential requirement for dpp signaling in initiation and propagation of morphogenesis in the Drosophila eye. Development, 122, 2153-2162.
38. Wrana, J.L. and Attisano, L. (1996) MAD-related proteins in TGFβ signalling. Trends Genet., 12, 493-496.
39. Wu, R.-Y., Zhang, Y., Feng, X.-H. and Derynck, R. (1997) Heteromeric and homomeric interactions correlate with signaling activity and functional cooperativity of Smad3 and Smad4/DPC4. Mol. Cell. Biol., 17, 2521-2528.
40. Yingling, J.M., Das, P., Savage, C., Zhang, C., Padgett, R.W. and Wang, X.-F. (1996) Mammalian Dwarfins are phosphorylated in response to TGFβ and are implicated in control of cell growth. Proc. Natl Acad. Sci. USA, 93, 8940-8944.
41. Zhang, Y., Feng, X.-H., Wu, R.-Y. and Derynck, R. (1996) Receptor-associated Mad homologues synergize as effectors of the TGFβ response. Nature, 383, 168-172.
42. Zhang, Y., Musci, T. and Derynck, R. (1997) The tumor suppressor Smad4/ DPC4 as a central mediator of Smad function. Curr. Biol., 7, 270-276.