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Volumn 37, Issue 20, 1998, Pages 2872-2875

Expanding the potential of DNA for binding and catalysis: Highly functionalized dUTP derivatives that are substrates for thermostable DNA polymerases

Author keywords

Aptamers; Combinatorial chemistry; DNA enzymes; In vitro selection; Nucleotides

Indexed keywords

DEOXYURIDINE TRIPHOSPHATE DERIVATIVE; DNA DIRECTED RNA POLYMERASE;

EID: 0032476840     PISSN: 14337851     EISSN: None     Source Type: Journal    
DOI: 10.1002/(SICI)1521-3773(19981102)37:20<2872::AID-ANIE2872>3.0.CO;2-5     Document Type: Article
Times cited : (158)

References (48)
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    • Catalytic nucleic acids: a) R. R. Breaker, Chem. Rev. 1997, 97, 371-390;
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    • Angew. Chem. Int. Ed. Engl. 1997, 36, 1879-1881. These authors State that "when modified oligomers are incorporated, the selection procedure is terminated after just one round of amplification since polymerases will not tolerate most modified mononucleotides".
    • (1997) Angew. Chem. Int. Ed. Engl. , vol.36 , pp. 1879-1881
  • 38
    • 0000477103 scopus 로고
    • Compound 3 was synthesized previously from deoxyuridine triphosphate (dUTP), however, problems with by-products and the expense of dUTP as a starting material posed serious obstacles for this synthetic route. P. R. Langer, A. A. Waldrop, D. C. Ward, Proc. Natl. Acad. Sci. U.S.A. 1981, 78, 6633-7.
    • (1981) Proc. Natl. Acad. Sci. U.S.A. , vol.78 , pp. 6633-6637
    • Langer, P.R.1    Waldrop, A.A.2    Ward, D.C.3
  • 39
    • 0003610959 scopus 로고
    • Several derivatives of 3 have been shown to be substrates for E. coli DNA polymerase and useful in nick translation and random primed synthesis when they replace dTTP. Homogeneous incorporation of these derivatives in PCR is not possible because of chain termination, see ref. [11c] for a discussion of derivatives in PCR. a) M. Shimkus, J. Levy, T. Herman, Proc. Natl. Acad. Sci. U.S.A. 1985, 82, 2593-7;
    • (1985) Proc. Natl. Acad. Sci. U.S.A. , vol.82 , pp. 2593-2597
    • Shimkus, M.1    Levy, J.2    Herman, T.3
  • 42
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    • PCR products with incorporated modified nucleotide analogues were cloned into the vector pCR2.1TOPO with the topoisomerase-activated vector provided from the manufacturer (Invitrogen)
    • PCR products with incorporated modified nucleotide analogues were cloned into the vector pCR2.1TOPO with the topoisomerase-activated vector provided from the manufacturer (Invitrogen).
  • 44
    • 0031570666 scopus 로고    scopus 로고
    • Reviews of the structures of nucleic acids that were selected to bind ligands detail the differences between the ligand-binding pockets of nucleic acids and proteins, see a) K. A. Marshall, M. P. Robertson, A. D. Ellington, Structure 1997, 5, 729-734;
    • (1997) Structure , vol.5 , pp. 729-734
    • Marshall, K.A.1    Robertson, M.P.2    Ellington, A.D.3
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    • b) M. Egli, Angew. Chem. 1997, 109, 494-497;
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    • Egli, M.1


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.