Cofactor-Assisted Self-Cleavage in DNA Libraries with a 3′-5′-Phosphoramidate Bond

Angewandte Chemie (International Edition in English)

Volumn 36, Issue 12, 1997, Pages 1321-1324

  Burmeister, Jens ^a   Von Kiedrowski, Günter ^a   Ellington, Andrew D ^b  

^a RUHR UNIVERSITY BOCHUM   (Germany)

^b INDIANA UNIVERSITY   (United States)

Author keywords

Combinatorial chemistry; DNA cleavage; Ribozymes

Indexed keywords

EID: 0030794322     PISSN: 05700833     EISSN: None     Source Type: Journal    
DOI: 10.1002/anie.199713211     Document Type: Article

References (32)

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22. N3-CCGpnT (1) and pCGG-dansyl (2) were synthesized in solution by the standard phosphotriester method.
23. 2) were followed in a 0.1 M solution of the following buffers: sodium citrate (pH = 3, 4, and 5); 2-(N-morpholino)ethanesulfonic acid, sodium salt (pH = 6); N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid), sodium salt (pH = 7).
24. a) The modified primer B was prepared on an Applied Biosystems 391 DNA Syntheziser with the phosphoramidite biotin TEG (Glen Research) and the phosphoramidite of 5′-N-(4-monomethoxytrityl)amino-2′,5′-dideoxythymidine;
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26. Negative selection was performed by incubation of the immobilized single-stranded pool in the selection buffer for 2.5 h in round 7 and 1 h in rounds 8-10. Following incubation the beads were washed several times with bidistilled water to remove released aminooligomers. Positive selection was performed according to the conditions described above (see legend of Figure 2).
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28. N.
29. The second clone had the following sequence: 5′- CCGpnTGGATCCTAATGACCAAGGCATAGCCTTTTAATGGATCCTCAGAAGGGGCTTGGTCCCGCCTGCGTCTAAACAAC ACGCTACGGCTATTTCGGTGCCAGTCGGATAGTGTT-3′
30. The program was invoked with the World Wide Web at http://www.ibc. wustl.edu/~zuker/dna/form1.cgi.
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32. -1. Upon incubation of selected pools in the presence of 2 and the template at pH = 6, more than 50% hydrolyzed product was detected after 16 h. Under the assumption that the whole pool can be hydrolyzed we conclude that the catalyzed reaction is about three orders of magnitude faster than uncatalyzed hydrolysis.