In vitro selection without intervening amplification

Angewandte Chemie (International Edition in English)

Volumn 36, Issue 17, 1997, Pages 1879-1881

  Smith, Joseph ^a   Anslyn, Eric V ^a  

^a UNIVERSITY OF TEXAS AT AUSTIN   (United States)

Author keywords

Aptamers; Combinatorial chemistry; In vitro selection; Nucleotides; Polymerase chain reaction

Indexed keywords

EID: 0030799729     PISSN: 05700833     EISSN: None     Source Type: Journal    
DOI: 10.1002/anie.199718791     Document Type: Article

References (5)

1. Some exceptions include 2′-amino- and 2′-fluoro substitutions on sugar (H. Aurup, D. M. Williams, F. Eckstein, Biochemistry 1992, 31, 9636-9641 ) and 1-pentynyl substitution on pyrimidine (J. A. Latham, R. Johnson, J. J. Toole, Nucleic Acids Res. 1994, 22, 2817-2822).
2. Some exceptions include 2′-amino- and 2′-fluoro substitutions on sugar (H. Aurup, D. M. Williams, F. Eckstein, Biochemistry 1992, 31, 9636-9641 ) and 1-pentynyl substitution on pyrimidine (J. A. Latham, R. Johnson, J. J. Toole, Nucleic Acids Res. 1994, 22, 2817-2822).
3. D. E. Huizenga, J. W. Szostak, Biochemistry 1995, 34, 656-665.
4. Oligonucleotides were purchased HPLC-purified from Operon. The initial sequence was 5′-GAATTCCAGATCTCT-(18N)-GATATCAGGATCCCA-3′. The two primers were 5′-GAATTCCAGATCTCT-3′ and 5′-TGGGATCCTGATATC-3′. These sequences incorporated Eco R I and BamH I digestion enzyme restriction sites.
5. D. M. Coen in Current Protocols in Molecular Biology (Eds. : F. M. Ausubel, R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, K. Struhl), Current Protocols, New York, 1994, Chapter 15.4.