Structural analysis of nucleic acid aptamers

Current Opinion in Chemical Biology

Volumn 1, Issue 1, 1997, Pages 32-46

  Patel, Dinshaw J ^a  

^a MEMORIAL SLOAN KETTERING CANCER CENTER   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

BIOPOLYMER; NUCLEIC ACID;

CHEMISTRY; CONFORMATION; REVIEW;

BIOPOLYMERS; NUCLEIC ACID CONFORMATION; NUCLEIC ACIDS;

EID: 0031152162     PISSN: 13675931     EISSN: None     Source Type: Journal    
DOI: 10.1016/s1367-5931(97)80106-8     Document Type: Article

References (103)

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84. Klussmann S, Nolte A, Bald R, Erdmann VA, Furst JP: Mirror image RNA that binds D-adenosine. Nat Biotechnol 1996, 14:1112-1115. The identification of L-RNA aptamers that target D-adenosine and D-RNA aptamers that target L-adenosine are reported in this paper. The RNA aptamers exhibited reciprocal chiral specificity in that the L-RNA aptamer discriminates by four orders of magnitude between D- and L-adenosine. The nonnatural L-RNA aptamer was extraordinarily stable in human serum.
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101. Allen P, Collins B, Brown D, Hostomsky Z, Gold L: A specific RNA structural motif mediates high affinity binding by the HIV-1 nucleocapsid protein (NCp7). Virology 1996, 225:306-315. This paper describes the identification of an RNA aptamer through in vitro selection for the HIV-1 nucleocapsid protein. The particular selection approach used yielded an RNA aptamer containing a stem-loop fold with an invariant four base bulge and a single-stranded segment.
102. Berglund JA, Charpentier B, Rosbash M: A high affinity binding site for the HIV-1 nucleocapsid protein. Nucleic Acids Res 1996, 25:1042-1049. This study describes the identification of an RNA aptamer through in vitro selection for the HIV-1 nucleocapsid protein. The particular selection approach used yielded a stem-loop RNA aptamer sequence containing an invariant asymmetric internal loop. Specific RNA residues in the stem, hairpin and asymmetric internal loops participate in complex formation.
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