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Volumn 10, Issue 2, 1998, Pages 188-195

B-cell lymphopoiesis in mouse and man

Author keywords

[No Author keywords available]

Indexed keywords

LYMPHOCYTE MEMBRANE RECEPTOR; MEMBRANE PROTEIN; SECRETORY PROTEIN;

EID: 0031956760     PISSN: 09527915     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0952-7915(98)80248-3     Document Type: Article
Times cited : (28)

References (68)
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    • of special interest. This intriguing paper introduces Polycomb group (Pc-G) gene function into the IL-7 proliferation equation. Pc-G genes in Drosophila melanogaster are transcription factors that regulate expression of HOM-C genes, which in turn are induced by segmentation gene products such as Kruppel and hunchback. The outcome of these complex interactions includes the determination of anterior-posterior segment identity in the fly. Vertebrate homologs of Pc-G have been identified, and include mel-18 and bmi-1. Evidence from several laboratories suggested a possible role for bmi-1 in hematopoietic cell proliferation. The current study examined lymphoid growth in mel-18-deficient mice. In addition to skeletal and smooth muscle abnormalities, mel-18-deficient mice exhibited defects in B-cell and T-cell lymphopoiesis that resembled the defects which occur in IL-7-deficient
    • of rest Akusaka T, Tsuji K-I, Kawakira H, Kanno M, Harigaya K-I, Hu L, Ebihara Y, Nakahata T, Tetsu O, Taniguchi M, Koseki H. The role of mel-18, a mammalian polycomb group gene, during IL-7-dependent proliferation of lymphocyte precursors. Immunity. 7:1997;135-146 This intriguing paper introduces Polycomb group (Pc-G) gene function into the IL-7 proliferation equation. Pc-G genes in Drosophila melanogaster are transcription factors that regulate expression of HOM-C genes, which in turn are induced by segmentation gene products such as Kruppel and hunchback. The outcome of these complex interactions includes the determination of anterior-posterior segment identity in the fly. Vertebrate homologs of Pc-G have been identified, and include mel-18 and bmi-1. Evidence from several laboratories suggested a possible role for bmi-1 in hematopoietic cell proliferation. The current study examined lymphoid growth in mel-18-deficient mice. In addition to skeletal and smooth muscle abnormalities, mel-18-deficient mice exhibited defects in B-cell and T-cell lymphopoiesis that resembled the defects which occur in IL-7-deficient mice. IL-7-dependent proliferation of thymocytes and pre-B-cell colony formation were reduced in mel-18-deficient mice; however, analysis of the expression and function of IL-7 signaling pathway molecules (γ chain, Jak3, STATS) revealed no detectable deficiencies in mel-18-deficient mice. Thus, mel-18 is important in IL-7 dependent proliferation, but acts potentially downstream of, or parallel to, Jak/STAT function.
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    • Matrix glycoprotein SC1/ECM2 augments B lymphopoiesis
    • of special interest. Oritani and Kincade have previously developed a novel cloning strategy that selects for stromal cell molecules that bind to the surface of pre-B cells. This study confirms the utility of that cloning strategy, by demonstrating that secreted and membrane forms of a matrix glycoprotein designated SC1/ECM2 can potentiate the clonogenicity of IL-7-dependent pre-B cells.
    • of special interest Oritani K, Kanakura Y, Aoyama K, Yokota T, Copeland NG, Gilbert DJ, Jenkins NA, Tomiyama Y, Matsuzawa Y, Kincade PW. Matrix glycoprotein SC1/ECM2 augments B lymphopoiesis. Blood. 90:1997;3404-3413 Oritani and Kincade have previously developed a novel cloning strategy that selects for stromal cell molecules that bind to the surface of pre-B cells. This study confirms the utility of that cloning strategy, by demonstrating that secreted and membrane forms of a matrix glycoprotein designated SC1/ECM2 can potentiate the clonogenicity of IL-7-dependent pre-B cells.
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    • The chemokine SDF-1, stromal cell-derived factor 1, attracts early stage B cell precursors via the chemokine receptor CXCR4
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    • + population, CD19- (i.e., Hardy fraction A) cells exhibited the strongest chemotactic response to SDF-1. Analysis of human pro-B leukemic, pre-B leukemic and Burkitt lymphoma cell lines revealed both a chemotactic response and a transient rise in intracellular calcium response to SDF-1 in the pro-B and pre-B cells. Importantly, an antibody to the CXCR4 chemokine receptor for SDF-1 blocked migration. This report provides the first evidence that B cell precursors exhibit chemotactic responses to a specific chemokine.
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