-
1
-
-
0029997936
-
Protein purification in the nineties
-
Scopes RK. Protein purification in the nineties. Biotechnol Appl Biochem. 23:1996;197-204.
-
(1996)
Biotechnol Appl Biochem
, vol.23
, pp. 197-204
-
-
Scopes, R.K.1
-
2
-
-
0017288609
-
The kinetics of protein salting-out: Precipitation of yeast enzymes by ammonium sulfate
-
Foster PR, Dunnill P, Lilly MD. The kinetics of protein salting-out: precipitation of yeast enzymes by ammonium sulfate. Biotechnol Bioeng. 28:1976;545-580.
-
(1976)
Biotechnol Bioeng
, vol.28
, pp. 545-580
-
-
Foster, P.R.1
Dunnill, P.2
Lilly, M.D.3
-
3
-
-
0031031150
-
Initial protein concentration effects on precipitation by salt
-
Shiau K-S, Chen T-L. Initial protein concentration effects on precipitation by salt. Biotechnol Bioeng. 53:1997;202-206.
-
(1997)
Biotechnol Bioeng
, vol.53
, pp. 202-206
-
-
Shiau K-S1
Chen T-L2
-
4
-
-
0027113003
-
Some characteristics of protein precipitation by salts
-
Shih Y-C, Prausnitz JM, Blanch HW. Some characteristics of protein precipitation by salts. Biotechnol Bioeng. 40:1992;1155-1164.
-
(1992)
Biotechnol Bioeng
, vol.40
, pp. 1155-1164
-
-
Shih Y-C1
Prausnitz, J.M.2
Blanch, H.W.3
-
5
-
-
30244510096
-
Salt-induced protein precipitation: Phase equilibria from an equation of state
-
of special interest. The latest paper of a series from this group treating the thermodynamics of protein precipitation with a pseudo-single component equation-of-state approach. They use a protein - protein interaction potential that includes specific interactions and gives reasonable agreement with experimental results. The article also provides an entrée to their earlier work.
-
Kuehner DE, Blanch HW, Prausnitz JM. Salt-induced protein precipitation: phase equilibria from an equation of state. of special interest Fluid Phase Equil. 116:1996;140-147 The latest paper of a series from this group treating the thermodynamics of protein precipitation with a pseudo-single component equation-of-state approach. They use a protein - protein interaction potential that includes specific interactions and gives reasonable agreement with experimental results. The article also provides an entrée to their earlier work.
-
(1996)
Fluid Phase Equil
, vol.116
, pp. 140-147
-
-
Kuehner, D.E.1
Blanch, H.W.2
Prausnitz, J.M.3
-
6
-
-
1842328003
-
Protein salting-out: Phase equilibria in two-protein systems
-
of special interest. Interactions between unlike proteins in solution can cause striking deviations of individual protein solubility behavior relative to that in the corresponding single protein solutions.
-
Coen CJ, Prausnitz JM, Blanch HW. Protein salting-out: phase equilibria in two-protein systems. of special interest Biotechnol Bioeng. 53:1997;567-574 Interactions between unlike proteins in solution can cause striking deviations of individual protein solubility behavior relative to that in the corresponding single protein solutions.
-
(1997)
Biotechnol Bioeng
, vol.53
, pp. 567-574
-
-
Coen, C.J.1
Prausnitz, J.M.2
Blanch, H.W.3
-
7
-
-
0028372337
-
Protein precipitation: Effects of mixing on protein solubility
-
Iyer H, Przybycien TM. Protein precipitation: effects of mixing on protein solubility. AIChE J. 40:1994;349-360.
-
(1994)
AIChE J
, vol.40
, pp. 349-360
-
-
Iyer, H.1
Przybycien, T.M.2
-
8
-
-
0030736197
-
Phase equilibria for the mean-force potential of globular protein solutions
-
of special interest. A powerful coupling of Monte Carlo simulation and perturbation theory that is qualitatively predictive of observed precipitation behavior.
-
Tavares FW, Sandler SI. Phase equilibria for the mean-force potential of globular protein solutions. of special interest AIChE J. 43:1997;218-231 A powerful coupling of Monte Carlo simulation and perturbation theory that is qualitatively predictive of observed precipitation behavior.
-
(1997)
AIChE J
, vol.43
, pp. 218-231
-
-
Tavares, F.W.1
Sandler, S.I.2
-
9
-
-
0030244193
-
Thermodynamics of precipitation of globular proteins by nonionic polymers
-
of special interest. This is a very accessible treatment of the application of perturbation theory to the prediction of phase behavior. The paper includes fitting of the model to actual experimental solubility data.
-
Guo R, Guo M, Narsimhan G. Thermodynamics of precipitation of globular proteins by nonionic polymers. of special interest Ind Eng Chem Res. 35:1996;3015-3026 This is a very accessible treatment of the application of perturbation theory to the prediction of phase behavior. The paper includes fitting of the model to actual experimental solubility data.
-
(1996)
Ind Eng Chem Res
, vol.35
, pp. 3015-3026
-
-
Guo, R.1
Guo, M.2
Narsimhan, G.3
-
10
-
-
0030189731
-
A model for the prediction of precipitation curves for globular proteins with nonionic polymers as the precipitation agent
-
Guo M, Narsimhan G. A model for the prediction of precipitation curves for globular proteins with nonionic polymers as the precipitation agent. Sep Sci Technol. 31:1996;1777-1804.
-
(1996)
Sep Sci Technol
, vol.31
, pp. 1777-1804
-
-
Guo, M.1
Narsimhan, G.2
-
11
-
-
0030169669
-
Phase behavior of globular protein and flexible polymer in an aqueous medium
-
of special interest. They take phase behavior modeling in non-ionic polymer precipitation to the next level by the treating protein and polymer as separate components.
-
Yu M, de Swaan Arons J. Phase behavior of globular protein and flexible polymer in an aqueous medium. of special interest Fluid Phase Equil. 120:1996;121-129 They take phase behavior modeling in non-ionic polymer precipitation to the next level by the treating protein and polymer as separate components.
-
(1996)
Fluid Phase Equil
, vol.120
, pp. 121-129
-
-
Yu, M.1
De Swaan Arons, J.2
-
12
-
-
0343907278
-
Whey protein fractionation: Isoelectric precipitation of α-lactalbumin under gentle heat treatment
-
Bramaud C, Aimar P, Daufin G. Whey protein fractionation: isoelectric precipitation of α-lactalbumin under gentle heat treatment. Biotechnol Bioeng. 56:1997;391-397.
-
(1997)
Biotechnol Bioeng
, vol.56
, pp. 391-397
-
-
Bramaud, C.1
Aimar, P.2
Daufin, G.3
-
14
-
-
51249166058
-
Affinity thermoprecipitation of trypsin using soybean trypsin inhibitor conjugated with a thermoreactive polymer, poly(N-vinyl caprolactam)
-
Galaev IY, Mattiasson B. Affinity thermoprecipitation of trypsin using soybean trypsin inhibitor conjugated with a thermoreactive polymer, poly(N-vinyl caprolactam). Biotechnol Tech. 6:1992;353-358.
-
(1992)
Biotechnol Tech
, vol.6
, pp. 353-358
-
-
Galaev, I.Y.1
Mattiasson, B.2
-
15
-
-
0029395001
-
Protein purification by bulk crystallization: The recovery of ovalbumin
-
Judge RA, John MR, White ET. Protein purification by bulk crystallization: the recovery of ovalbumin. Biotechnol Bioeng. 48:1995;316-323.
-
(1995)
Biotechnol Bioeng
, vol.48
, pp. 316-323
-
-
Judge, R.A.1
John, M.R.2
White, E.T.3
-
16
-
-
0030861664
-
Use of a fusion protein to obtain crystals suitable for x-ray analysis: Crystallization of a GST-fused protein containing the DNA-binding domain of DNA replication-related element-binding factor, DREF
-
Kuge M, Fuii Y, Shimizu T, Hirose F, Matsukage A, Hakoshima T. Use of a fusion protein to obtain crystals suitable for x-ray analysis: crystallization of a GST-fused protein containing the DNA-binding domain of DNA replication-related element-binding factor, DREF. Protein Sci. 6:1997;1783-1786.
-
(1997)
Protein Sci
, vol.6
, pp. 1783-1786
-
-
Kuge, M.1
Fuii, Y.2
Shimizu, T.3
Hirose, F.4
Matsukage, A.5
Hakoshima, T.6
-
17
-
-
10144225635
-
Self-interaction chromatography: A tool for the study of protein - Protein interactions in bioprocessing environments
-
of special interest. A brief review of common themes in protein - protein interaction phenomena is given in addition to a description of the SIC technique. Analytical applications of SIC are described including the identification of binding sites and the screening of excipients.
-
Patro SY, Przybycien TM. Self-interaction chromatography: a tool for the study of protein - protein interactions in bioprocessing environments. of special interest Biotechnol Bioeng. 52:1996;193-203 A brief review of common themes in protein - protein interaction phenomena is given in addition to a description of the SIC technique. Analytical applications of SIC are described including the identification of binding sites and the screening of excipients.
-
(1996)
Biotechnol Bioeng
, vol.52
, pp. 193-203
-
-
Patro, S.Y.1
Przybycien, T.M.2
-
18
-
-
0026751770
-
Immobilized hemoglobin in the purification of hemoglobin-based oxygen carriers
-
Chiancone E, Fronticelli C, Gattoni M, Urbatis B, Bucci E. Immobilized hemoglobin in the purification of hemoglobin-based oxygen carriers. J Chromtogr. 604:1992;117-123.
-
(1992)
J Chromtogr
, vol.604
, pp. 117-123
-
-
Chiancone, E.1
Fronticelli, C.2
Gattoni, M.3
Urbatis, B.4
Bucci, E.5
-
19
-
-
0031570359
-
Low-molecular-weight displacers for high resolution protein separations
-
Kundu A, Cramer SM. Low-molecular-weight displacers for high resolution protein separations. Anal Biochem. 248:1997;111-116.
-
(1997)
Anal Biochem
, vol.248
, pp. 111-116
-
-
Kundu, A.1
Cramer, S.M.2
-
20
-
-
0027561441
-
Affinity-based reverse micellar extraction and separation (ARMES): A facile technique for the purification of peroxidase from soybean hulls
-
Paradkar VM, Dordick JS. Affinity-based reverse micellar extraction and separation (ARMES): a facile technique for the purification of peroxidase from soybean hulls. Biotechnol Prog. 9:1993;199-203.
-
(1993)
Biotechnol Prog
, vol.9
, pp. 199-203
-
-
Paradkar, V.M.1
Dordick, J.S.2
-
21
-
-
0031194058
-
Parameters affecting the efficiency of affinity-based reversed micellar extraction and separation (ARMES) in glycoprotein purification
-
Choe J, VanderNoot VA, Linhardt RJ, Dordick JS. Parameters affecting the efficiency of affinity-based reversed micellar extraction and separation (ARMES) in glycoprotein purification. Biotechnol Prog. 13:1997;440-445.
-
(1997)
Biotechnol Prog
, vol.13
, pp. 440-445
-
-
Choe, J.1
Vandernoot, V.A.2
Linhardt, R.J.3
Dordick, J.S.4
-
22
-
-
0342894714
-
Bioaffinity separation of trypsin using trypsin inhibitor immobilized in reverse micelles composed of a nonionic surfactant
-
Adachi M, Yamazaki M, Harada M, Shioi A, Katch S. Bioaffinity separation of trypsin using trypsin inhibitor immobilized in reverse micelles composed of a nonionic surfactant. Biotechnol Bioeng. 53:1997;406-408.
-
(1997)
Biotechnol Bioeng
, vol.53
, pp. 406-408
-
-
Adachi, M.1
Yamazaki, M.2
Harada, M.3
Shioi, A.4
Katch, S.5
-
23
-
-
0030042260
-
Porous zirconia: A new support material for enzyme immobilization
-
Huckel M, Wirth H-J, Hearn MTW. Porous zirconia: a new support material for enzyme immobilization. J Biochem Biophys Methods. 31:1996;165-179.
-
(1996)
J Biochem Biophys Methods
, vol.31
, pp. 165-179
-
-
Huckel, M.1
Wirth H-J2
Hearn, M.T.W.3
-
24
-
-
0028967513
-
Gel fiberglass as a new support for affinity chromatography
-
Zusman R, Zusman I. Gel fiberglass as a new support for affinity chromatography. Biotechnol Appl Biochem. 21:1995;161-172.
-
(1995)
Biotechnol Appl Biochem
, vol.21
, pp. 161-172
-
-
Zusman, R.1
Zusman, I.2
-
25
-
-
0029973638
-
Gel fiberglass as a new matrix of affinity chromatography columns for isolation of colon cancer-associated antigens
-
Zusman I, Zusman R, Sandler B, Korol D, Gurevich P, Smirnoff P, Bass D, Or A, Shani A, Idelevich E. Gel fiberglass as a new matrix of affinity chromatography columns for isolation of colon cancer-associated antigens. Int J Oncology. 9:1996;153-157.
-
(1996)
Int J Oncology
, vol.9
, pp. 153-157
-
-
Zusman, I.1
Zusman, R.2
Sandler, B.3
Korol, D.4
Gurevich, P.5
Smirnoff, P.6
Bass, D.7
Or, A.8
Shani, A.9
Idelevich, E.10
-
26
-
-
84985331666
-
Design and preparation of novel particulate and continuous polymeric macroporous media for the separation of biological and synthetic molecules
-
Frechet JMJ. Design and preparation of novel particulate and continuous polymeric macroporous media for the separation of biological and synthetic molecules. Makromol Chem Macromol Symp. 70/71:1993;289-301.
-
(1993)
Makromol Chem Macromol Symp
, vol.7071
, pp. 289-301
-
-
Frechet, J.M.J.1
-
27
-
-
0030087802
-
Immobilization of trypsin onto molded macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) rods and use of conjugates as bioreactors and for affinity chromatography
-
Petro M, Svec F, Frechet JMJ. Immobilization of trypsin onto molded macroporous poly(glycidyl methacrylate-co-ethylene dimethacrylate) rods and use of conjugates as bioreactors and for affinity chromatography. Biotechnol Bioeng. 49:1996;355-363.
-
(1996)
Biotechnol Bioeng
, vol.49
, pp. 355-363
-
-
Petro, M.1
Svec, F.2
Frechet, J.M.J.3
-
28
-
-
0031172715
-
Expanded-bed adsorption in industrial bioprocessing: Recent developments
-
Hjorth R. Expanded-bed adsorption in industrial bioprocessing: recent developments. Trends Biotechnol. 15:1997;230-235.
-
(1997)
Trends Biotechnol
, vol.15
, pp. 230-235
-
-
Hjorth, R.1
-
29
-
-
0030298456
-
Isolation of monoclonal antibodies from cell containing hybridoma broth using a protein A coated adsorbent in expanded beds
-
Thommes J, Bader A, Halfar M, Karau A, Kula M-R. Isolation of monoclonal antibodies from cell containing hybridoma broth using a protein A coated adsorbent in expanded beds. J Chromatogr. 752:1996;111-122.
-
(1996)
J Chromatogr
, vol.752
, pp. 111-122
-
-
Thommes, J.1
Bader, A.2
Halfar, M.3
Karau, A.4
Kula M-R5
-
30
-
-
51249162494
-
Genetic engineering to enhance the selectivity of protein separations
-
Glatz CE, Ford CF. Genetic engineering to enhance the selectivity of protein separations. Appl Biochem Biotechnol. 54:1995;173-191.
-
(1995)
Appl Biochem Biotechnol
, vol.54
, pp. 173-191
-
-
Glatz, C.E.1
Ford, C.F.2
-
31
-
-
0029807108
-
Bifunctional fusion proteins of calmodulin and protein A as affinity ligands in protein purification and in the study of protein - Protein interactions
-
of outstanding interest. A bifunctional fusion protein can be used in a traditional affinity chromatography capture step or can be co-expressed with the target protein to bind in vivo for subsequent chromatographic isolation. The ligand may also be removed and reloaded without repacking the chromatography column.
-
Hentz NG, Daunert S. Bifunctional fusion proteins of calmodulin and protein A as affinity ligands in protein purification and in the study of protein - protein interactions. of outstanding interest Anal Chem. 68:1996;3939-3944 A bifunctional fusion protein can be used in a traditional affinity chromatography capture step or can be co-expressed with the target protein to bind in vivo for subsequent chromatographic isolation. The ligand may also be removed and reloaded without repacking the chromatography column.
-
(1996)
Anal Chem
, vol.68
, pp. 3939-3944
-
-
Hentz, N.G.1
Daunert, S.2
-
33
-
-
0030808309
-
Introduction of spacer peptides N-terminal to a cleavage recognition motif in recombinant fusion proteins can improve site-specific cleavage
-
Polyak SW, Forsberg G, Forbes BE, McNeil KA, Aplin SE, Wallace JC. Introduction of spacer peptides N-terminal to a cleavage recognition motif in recombinant fusion proteins can improve site-specific cleavage. Protein Eng. 10:1997;615-619.
-
(1997)
Protein Eng
, vol.10
, pp. 615-619
-
-
Polyak, S.W.1
Forsberg, G.2
Forbes, B.E.3
McNeil, K.A.4
Aplin, S.E.5
Wallace, J.C.6
-
34
-
-
0029876212
-
Single-step trypsin cleavage of a fusion protein to obtain human insulin and its C peptide
-
of special interest. Exotic enzymes need not be used to cleave fusion proteins. This is a classic example of thinking outside the box.
-
Jonasson P, Nilsson J, Samuelsson E, Moks T, Stahl S, Uhlen M. Single-step trypsin cleavage of a fusion protein to obtain human insulin and its C peptide. of special interest Eur J Biochem. 236:1996;656-661 Exotic enzymes need not be used to cleave fusion proteins. This is a classic example of thinking outside the box.
-
(1996)
Eur J Biochem
, vol.236
, pp. 656-661
-
-
Jonasson, P.1
Nilsson, J.2
Samuelsson, E.3
Moks, T.4
Stahl, S.5
Uhlen, M.6
-
35
-
-
17744415288
-
Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element
-
of outstanding interest. The use of inteins as self-splicing fusion protein linkers is demonstrated for the isolation of nine different target proteins. A mechanism for the splicing reaction is also proposed. The use of intein linkers will probably become standard fusion protein technology.
-
Chong S, Mersha FB, Comb DG, Scott ME, Landry D, Vence LM, Perler FB, Benner J, Kucera RB, Hiroven CA, et al. Single-column purification of free recombinant proteins using a self-cleavable affinity tag derived from a protein splicing element. of outstanding interest Gene. 192:1997;271-281 The use of inteins as self-splicing fusion protein linkers is demonstrated for the isolation of nine different target proteins. A mechanism for the splicing reaction is also proposed. The use of intein linkers will probably become standard fusion protein technology.
-
(1997)
Gene
, vol.192
, pp. 271-281
-
-
Chong, S.1
Mersha, F.B.2
Comb, D.G.3
Scott, M.E.4
Landry, D.5
Vence, L.M.6
Perler, F.B.7
Benner, J.8
Kucera, R.B.9
Hiroven, C.A.10
-
36
-
-
0030803760
-
Genetic definition of a protein-splicing domain: Functional mini-inteins support structure predictions and a model for intein evolution
-
of special interest. Only a portion of a wild-type intein may be necessary to retain self-splicing capability.
-
Derbyshire V, Wood DW, Wu W, Dansereau JT, Dalgaard JZ, Belfort M. Genetic definition of a protein-splicing domain: functional mini-inteins support structure predictions and a model for intein evolution. of special interest Proc Natl Acad Sci USA. 94:1997;11466-11471 Only a portion of a wild-type intein may be necessary to retain self-splicing capability.
-
(1997)
Proc Natl Acad Sci USA
, vol.94
, pp. 11466-11471
-
-
Derbyshire, V.1
Wood, D.W.2
Wu, W.3
Dansereau, J.T.4
Dalgaard, J.Z.5
Belfort, M.6
-
37
-
-
0031144784
-
Immobilized Cratylia mollis lectin as a potential matrix to isolate plasma glycoproteins, including lecithincholesterol acyl transferase
-
Lima VLM, Correia MTS, Cechinel YMN, Sampaio CAM, Owen JS, Coelho LCBB. Immobilized Cratylia mollis lectin as a potential matrix to isolate plasma glycoproteins, including lecithincholesterol acyl transferase. Carbohydr Polym. 33:1997;27-32.
-
(1997)
Carbohydr Polym
, vol.33
, pp. 27-32
-
-
Lima, V.L.M.1
Correia, M.T.S.2
Cechinel, Y.M.N.3
Sampaio, C.A.M.4
Owen, J.S.5
Coelho, L.C.B.B.6
-
38
-
-
0030835822
-
Binding proteins selected from combinatorial libraries of an α-helical bacterial receptor domain
-
of outstanding interest. Small protein binding domains, termed affibodies, are selected for use as potential affinity chromatography ligands by monovalent phage display technology.
-
Nord K, Gunneriusson E, Ringdahl J, Stahl S, Uhlen M, Nygren P-A. Binding proteins selected from combinatorial libraries of an α-helical bacterial receptor domain. of outstanding interest Nat Biotechnol. 15:1997;772-777 Small protein binding domains, termed affibodies, are selected for use as potential affinity chromatography ligands by monovalent phage display technology.
-
(1997)
Nat Biotechnol
, vol.15
, pp. 772-777
-
-
Nord, K.1
Gunneriusson, E.2
Ringdahl, J.3
Stahl, S.4
Uhlen, M.5
Nygren P-A6
-
39
-
-
0030994634
-
Yeast surface display for screening combinatorial libraries
-
of outstanding interest. An exposition of display technology employing yeast as a host organism; yeast have advantages of facile culture and of post-translational processing machinery that is similar to mammalian cells. They were able to select an scFv antibody fragment with threefold increased antigen binding constant relative to the wild type.
-
Boder ET, Wittrup KD. Yeast surface display for screening combinatorial libraries. of outstanding interest Nat Biotechnol. 15:1997;553-557 An exposition of display technology employing yeast as a host organism; yeast have advantages of facile culture and of post-translational processing machinery that is similar to mammalian cells. They were able to select an scFv antibody fragment with threefold increased antigen binding constant relative to the wild type.
-
(1997)
Nat Biotechnol
, vol.15
, pp. 553-557
-
-
Boder, E.T.1
Wittrup, K.D.2
-
40
-
-
0031012062
-
Display of heterologous proteins on the surface of microorganisms: From the screening of combinatorial libraries to live recombinant vaccines
-
of outstanding interest. A concise review of display technology using bacteria and yeast, giving a good entree to the field.
-
Georgiou G, Stathopoulos C, Daugherty PS, Nayak AR, Iverson BL, Curtiss R. Display of heterologous proteins on the surface of microorganisms: from the screening of combinatorial libraries to live recombinant vaccines. of outstanding interest Nat Biotechnol. 15:1997;29-34 A concise review of display technology using bacteria and yeast, giving a good entree to the field.
-
(1997)
Nat Biotechnol
, vol.15
, pp. 29-34
-
-
Georgiou, G.1
Stathopoulos, C.2
Daugherty, P.S.3
Nayak, A.R.4
Iverson, B.L.5
Curtiss, R.6
-
41
-
-
0029850976
-
Engineering a de novo-designed coiled coil heterodimerization domain for the rapid detection, purification and characterization of recombinantly expressed peptides and proteins
-
of outstanding interest. A universal fusion tag system is described for use in affinity chromatography systems based on a pair of peptides engineered to heterodimerize selectively in a coiled-coil configuration.
-
Tripet B, Yu L, Bautista DL, Wong WY, Irvin RT, Hodges RS. Engineering a de novo-designed coiled coil heterodimerization domain for the rapid detection, purification and characterization of recombinantly expressed peptides and proteins. of outstanding interest Protein Eng. 9:1996;1029-1042 A universal fusion tag system is described for use in affinity chromatography systems based on a pair of peptides engineered to heterodimerize selectively in a coiled-coil configuration.
-
(1996)
Protein Eng
, vol.9
, pp. 1029-1042
-
-
Tripet, B.1
Yu, L.2
Bautista, D.L.3
Wong, W.Y.4
Irvin, R.T.5
Hodges, R.S.6
|