Introduction of spacer peptides N-terminal to a cleavage recognition motif in recombinant fusion proteins can improve site-specific cleavage

Protein Engineering

Volumn 10, Issue 6, 1997, Pages 615-619

  Polyak, Steven W ^a   Forsberg, Göran ^b   Forbes, Briony E ^a   McNeil, Kerrie A ^a   Aplin, Sally E ^c   Wallace, John C ^a  

^a UNIVERSITY OF ADELAIDE   (Australia)

^b Pharmacia & Upjohn AB   (Sweden)

^c GroPep Ltd   (Australia)

Author keywords

H64A subtilisin; IGF II fusion proteins; Linkers; Protein digestion; Spacer peptides

Indexed keywords

HYBRID PROTEIN; PROTEINASE; SOMATOMEDIN B; SOMATREM; SUBTILISIN;

AMINO TERMINAL SEQUENCE; ARTICLE; CONTROLLED STUDY; PRIORITY JOURNAL; PROTEIN DEGRADATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN PURIFICATION; PROTEIN STRUCTURE;

SUIDAE;

EID: 0030808309     PISSN: 02692139     EISSN: None     Source Type: Journal    
DOI: 10.1093/protein/10.6.615     Document Type: Article

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