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Volumn 271, Issue 5256, 1996, Pages 1718-1723

Requirement for cAMP-PKA pathway activation by M phase-promoting factor in the transition from mitosis to interphase

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIC AMP; CYCLIC AMP DEPENDENT PROTEIN KINASE; CYCLIN B; CYCLINE;

EID: 0029964613     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5256.1718     Document Type: Article
Times cited : (129)

References (36)
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    • The GST-RII fusion protein was made by inserting after filling in with Klenow fragment (Promega), the Nde I fragment of the plasmid pET3a containing the rat PKA regulatory subunit type II β coding sequence (provided by C S Rubin) into the Sma I site o pGEX2T plasmid (Pharmacia) The GST-RII fusior protein was prepared as described (26) GST-RII was stored at 4°C in 20 mM tris (pH 7 4) and 50 mM NaCl and was used within 10 days of preparation
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    • We thank T. Hunt and A. W Murray for helpful discussions; M Pagano, G. Draetta, I Daar, G F Vande Woude, C. S Rubin, and S Obici for reagents; S. Scala for technical advice; and M. Berardone for artwork. Supported by Associazione Italiana per la Ricerca sul Cancro (AIRC), Consiglio Nazionale della Ricerca (Progetti Finalizzati Oncologia, Applicazioni Cliniche della Ricerca Oncologica e Ingegnena Genetica), and NIH D G was supported by an AIRC fellowship


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