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Volumn 271, Issue 5256, 1996, Pages 1718-1723

Requirement for cAMP-PKA pathway activation by M phase-promoting factor in the transition from mitosis to interphase

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIC AMP; CYCLIC AMP DEPENDENT PROTEIN KINASE; CYCLIN B; CYCLINE;

EID: 0029964613     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.271.5256.1718     Document Type: Article
Times cited : (127)

References (36)
  • 1
    • 0024342438 scopus 로고
    • J.-C. Labbé et al , EMBO J. 8, 3053 (1989); J Gautier et al., Cell 60, 487 (1990); P Nurse, Nature 344, 503 (1990)
    • (1989) EMBO J. , vol.8 , pp. 3053
    • Labbé, J.-C.1
  • 2
    • 0025012866 scopus 로고
    • J.-C. Labbé et al , EMBO J. 8, 3053 (1989); J Gautier et al., Cell 60, 487 (1990); P Nurse, Nature 344, 503 (1990)
    • (1990) Cell , vol.60 , pp. 487
    • Gautier, J.1
  • 3
    • 0025246110 scopus 로고
    • J.-C. Labbé et al , EMBO J. 8, 3053 (1989); J Gautier et al., Cell 60, 487 (1990); P Nurse, Nature 344, 503 (1990)
    • (1990) Nature , vol.344 , pp. 503
    • Nurse, P.1
  • 6
    • 0024978338 scopus 로고
    • 2. and 20 mM EGTA (pH 7.4)] and incubating for 10 min at 30°C. Histone H1 was separated in an SDS-12% polyacrylamide gel, and radioactivity was quantified by counting excised gel portions containing histone in a scintillation counter. Addition of PK1 to the histone H1 kinase reaction inhibited phosphorylation by less than 5%; a similar degree of inhibition was observed in experiments in which cAMP was added to the extracts (at the concentrations indicated in the legend to Fig. 3).
    • (1989) Nature , vol.339 , pp. 275
    • Murray, A.W.1    Kirschner, M.W.2
  • 10
    • 0026697250 scopus 로고
    • F. C. Luca, E. K. Shibuya, C. E Dohrmann, J V Ruderman, EMBO J. 10, 4311 (1991); T. Lorca et al , J Cell Sci. 102, 55 (1992).
    • (1992) J Cell Sci. , vol.102 , pp. 55
    • Lorca, T.1
  • 12
    • 0023613813 scopus 로고
    • D Kimelman, M. W. Kirschner, T. Scherson, ibid 48. 399 (1987), J. Gerhart, M Wu, M. W. Kirschner, J. Cell Biol. 98, 1247 (1984); K Hara, P Tydeman, M W. Kirschner, Proc. Natl. Acad Sci U S A. 77, 462 (1980); A. W Murray, Nature 359, 599 (1992)
    • (1987) Cell , vol.48 , pp. 399
    • Kimelman, D.1    Kirschner, M.W.2    Scherson, T.3
  • 13
    • 0021215746 scopus 로고
    • D Kimelman, M. W. Kirschner, T. Scherson, ibid 48. 399 (1987), J. Gerhart, M Wu, M. W. Kirschner, J. Cell Biol. 98, 1247 (1984); K Hara, P Tydeman, M W. Kirschner, Proc. Natl. Acad Sci U S A. 77, 462 (1980); A. W Murray, Nature 359, 599 (1992)
    • (1984) J. Cell Biol. , vol.98 , pp. 1247
    • Gerhart, J.1    Wu, M.2    Kirschner, M.W.3
  • 14
    • 0018845758 scopus 로고
    • D Kimelman, M. W. Kirschner, T. Scherson, ibid 48. 399 (1987), J. Gerhart, M Wu, M. W. Kirschner, J. Cell Biol. 98, 1247 (1984); K Hara, P Tydeman, M W. Kirschner, Proc. Natl. Acad Sci U S A. 77, 462 (1980); A. W Murray, Nature 359, 599 (1992)
    • (1980) Proc. Natl. Acad Sci U S A , vol.77 , pp. 462
    • Hara, K.1    Tydeman, P.2    Kirschner, M.W.3
  • 15
    • 0026703325 scopus 로고
    • D Kimelman, M. W. Kirschner, T. Scherson, ibid 48. 399 (1987), J. Gerhart, M Wu, M. W. Kirschner, J. Cell Biol. 98, 1247 (1984); K Hara, P Tydeman, M W. Kirschner, Proc. Natl. Acad Sci U S A. 77, 462 (1980); A. W Murray, Nature 359, 599 (1992)
    • (1992) Nature , vol.359 , pp. 599
    • Murray, A.W.1
  • 19
    • 13344253154 scopus 로고    scopus 로고
    • note
    • -32P]ATP (3000 Ci, mmol. Amersham) to 4 μl of extract diluted 1 : 50 in EB buffer (4) and incubatingfor 10 min at 30°C Where indicated, 20 μM PK1 synthetic peptide (amide, Gibco) was added to the reaction mixture. Kemptide was separated in an SDS-18% polyacrylamide gel, and radioactivity was quantified by counting excised gel portions containing Kemptide in a scintillation counter
  • 20
    • 13344251075 scopus 로고    scopus 로고
    • note
    • The GST-RII fusion protein was made by inserting after filling in with Klenow fragment (Promega), the Nde I fragment of the plasmid pET3a containing the rat PKA regulatory subunit type II β coding sequence (provided by C S Rubin) into the Sma I site o pGEX2T plasmid (Pharmacia) The GST-RII fusior protein was prepared as described (26) GST-RII was stored at 4°C in 20 mM tris (pH 7 4) and 50 mM NaCl and was used within 10 days of preparation
  • 21
    • 13344256378 scopus 로고    scopus 로고
    • unpublished data
    • A Porcelini. unpublished data
    • Porcelini, A.1
  • 22
    • 13344292198 scopus 로고    scopus 로고
    • unpublished data
    • D Grieco, unpublished data
    • Grieco, D.1
  • 23
    • 0025041797 scopus 로고
    • J Minshull, R Golsteyn, C S Hill, T Hunt, EMBO J 9, 2865 (1990). Cyclin A immunoprecipitations were done on extract samples (4 μl) diluted in EB buffer (4) containing 150 mM NaCl (100 μl) and cleared with protein G-agarose (Pharmacia) Antibody to Xenopus cyclin A (2 μl) was added to each sample, and the mixture was incubated for 6 hours at 4°C in constant rotation. Protein G-agarose was then added and incubation was continued for another hour The beads were washed three times with 0 5 ml of EB buffer containing 150 mM NaCl Proteins were eluted by boiling in SDS sample buffer and were separated on a 10% polyacrylamide gel The gel was dried and autoradiographed.
    • (1990) EMBO J , vol.9 , pp. 2865
    • Minshull, J.1    Golsteyn, R.2    Hill, C.S.3    Hunt, T.4
  • 24
    • 13344257167 scopus 로고    scopus 로고
    • note
    • One unit of PKA activity is equal to 1 pmol of phosphate transferred from ATP to Kemptide per minute at 30°C.
  • 25
    • 0027519780 scopus 로고
    • T Lorca et al , Nature 366, 270 (1993)
    • (1993) Nature , vol.366 , pp. 270
    • Lorca, T.1
  • 26
    • 13344255434 scopus 로고    scopus 로고
    • note
    • 35S]methionine (> 1000 Ci/mmol, Amersham) at 1 mCi/ml
  • 28
  • 33
    • 0017340457 scopus 로고
    • J. Finidon-Lepicard, S. Schorderet-Slatkine, J Hanoune, E.-E Baulieu, Nature 292, 255 (1981), J. L Maller and E G Krebs, J Biol Chem 252, 1712 (1977), S Schorderet-Slatkine, S. Schorderet, P Boquet, F. Godeau, E. E. Baulieu, Cell 15, 1269 (1978)
    • (1977) J Biol Chem , vol.252 , pp. 1712
    • Maller, J.L.1    Krebs, E.G.2
  • 36
    • 13344253153 scopus 로고    scopus 로고
    • note
    • We thank T. Hunt and A. W Murray for helpful discussions; M Pagano, G. Draetta, I Daar, G F Vande Woude, C. S Rubin, and S Obici for reagents; S. Scala for technical advice; and M. Berardone for artwork. Supported by Associazione Italiana per la Ricerca sul Cancro (AIRC), Consiglio Nazionale della Ricerca (Progetti Finalizzati Oncologia, Applicazioni Cliniche della Ricerca Oncologica e Ingegnena Genetica), and NIH D G was supported by an AIRC fellowship


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