Functional domains in nuclear import factor p97 for binding the nuclear localization sequence receptor and the nuclear pore

Molecular Biology of the Cell

Volumn 8, Issue 6, 1997, Pages 945-956

  Chi, Neil C ^a   Adam, Stephen A ^a  

^a NORTHWESTERN UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

NUCLEAR PROTEIN;

ARTICLE; CELL NUCLEUS; CELLULAR DISTRIBUTION; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; HUMAN; HUMAN CELL; INTRACELLULAR TRANSPORT; MOLECULAR RECOGNITION; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN DOMAIN; PROTEIN LOCALIZATION; PROTEIN TARGETING;

EID: 0030910804     PISSN: 10591524     EISSN: None     Source Type: Journal    
DOI: 10.1091/mbc.8.6.945     Document Type: Article

References (63)

1. Adam, E.J.H., and Adam, S.A. (1994). Identification of cytosolic factors required for nuclear location sequence-mediated binding to the nuclear envelope. J. Cell Biol. 125, 547-555.
2. Adam, S.A., Adam, E.J.H., Chi, N.C., and Visser, G.D. (1995). Cytoplasmic factors in NLS-mediated targeting to the nuclear pore complex. Cold Spring Harbor Symp. Quant. Biol. 60, 687-694.
3. Adam, S.A., and Gerace, L. (1991). Cytosolic proteins that specifically bind nuclear location signals are receptors for nuclear import. Cell 66, 837-847.
4. Adam, S.A., R.E. Sterne-Marr, and Gerace, L. (1990). Nuclear protein import in permeabilized mammalian cells requires soluble cytoplasmic factors. J. Cell Biol. 111, 807-816.
5. Aitchison, J.D., Blobel, G., and Rout, M.P. (1996). Kap104p: a karyopherin involved in the nuclear transport of messenger RNA binding proteins. Science 274, 624-627.
6. Becker, J., Melchior, F., Gerke, V., Bischoff, F.R., Ponstingl, H., and Wittinghofer, A. (1995). RNA1 encodes a GTPase-activating protein specific for Gsp1p, the Ran/TC4 homologue of Saccharomyces cerevisiae. J. Biol. Chem. 270, 11860-11865.
7. Beddow, A.L., Richards, S.A., Orem, N.R., and Macara, I.G. (1995). The Ran/TC4 GTPase-binding domain: Identification by expression cloning and characterization of a conserved sequence motif. Proc. Natl. Acad. Sci. USA 92, 3328-3332.
8. Bischoff, F.R., Klebe, C., Kretschmer, J., Wittinghofer, A., and Ponstingl, H. (1994). RanGAP1 induces GTPase activity of nuclear Ras-related Ran. Proc. Natl. Acad. Sci. USA 91, 2587-2591.
9. Bischoff, F.R., Krebber, H., Smirnova, E., Dong, W., and Ponstingl, H. (1995). Co-activation of RanGTPase and inhibition of GTP dissociation by Ran-GTP binding protein RanBP1. EMBO J. 14, 705-715.
10. Bischoff, F.R., and Ponstingl, H. (1991). Catalysis of guanine nucleotide exchange on Ran by the mitotic regulator RCC1. Nature 355, 80-82.
11. Bullock, T.L., Clarkson, W.D., Kent, H.M., and Stewart, M. (1996). The 1.6 angstrom resolution crystal structure of nuclear transport factor 2 (NTF2). J. Mol. Biol. 260, 422-431.
12. Carey, K.L., Richards, S.A., Lounsbury, K.M., and Macara, I.G. (1996). Evidence using a green fluorescent protein-glucocorticoid receptor chimera that the RAN/TC4 GTPase mediates an essential function independent of nuclear protein import. J. Cell Biol. 133, 985-996.
13. Chi, N.C., Adam, E.J.H., and Adam, S.A. (1995). Sequence and characterization of cytosolic nuclear protein import factor p97. J. Cell Biol. 130, 265-274.
14. Chi, N.C., Adam, E.J.H., and Adam, S.A. (1997). Different binding domains for Ran-GTP and Ran-GDP/RanBP1 on nuclear import factor p97. J. Biol. Chem. 272, 6818-6822.
15. Chi, N.C., Adam, E.J.H., Visser, G.D., and Adam, S.A. (1996). RanBP1 stabilizes the interaction of Ran with p97 in nuclear protein import. J. Cell Biol. 135, 1-11.
16. Corbett, A.H., Koepp, D.M., Schlenstedt, G., Lee, M.S., Hopper, A.K., and Silver, P.A. (1995). Rna1p, a Ran/TC4 GTPase activating protein, is required for nuclear import. J. Cell Biol. 130, 1017-1026.
17. Dingwall, C., and Laskey, R. (1991). Nuclear targeting sequences - a consensus? Trends Biochem. Sci. 16, 478-481.
18. Enenkel, C., Blobel, G., and Rexach, M. (1995). Identification of a yeast karyopherin heterodimer that targets import substrate to mammalian nuclear pore complexes. J. Biol. Chem. 270, 16499-16502.
19. Görlich, D., Henklein, P., Laskey, R.A., and Hartmann, E. (1996a). A 41 amino acid motif in importin-α confers binding to importin-β and hence transit into the nucleus. EMBO J. 15, 1810-1817.
20. Görlich, D., Kostka, S., Kraft, R., Dingwall, C., Laskey, R.A., Hartmann, E., and Prehn, S. (1995a). Two different subunits of importin cooperate to recognize nuclear localization signals and bind them to the nuclear envelope. Curr. Biol. 5, 383-392.
21. Görlich, D.S., and Mattaj, I.W. (1996). Nucleocytoplasmic transport. Science 271, 1513-1518.
22. Görlich, D., Panté, N., Kutay, U., Aebi, U., and Bischoff, F.R. (1996b). Identification of different roles for RanGDP and RanGTP in nuclear protein import. EMBO J. 15, 5584-5594.
23. Görlich, D.S., Prehn, S., Laskey, R.A., and Hartmann, E. (1994). Isolation of a protein that is essential for the first step of nuclear protein import. Cell 79, 767-778.
24. Görlich, D., Vogel, F., Mills, A.D., Hartmann, E., and Laskey, R.A. (1995b). Distinct functions for the two importin subunits in nuclear protein import. Nature 377, 246-248.
25. Guan, T., Muller, S., Klier, G., N. Panté, Blevitt, J.M., Haner, M., Paschal, B., Aebi, U., and Gerace, L. (1995). Structural analysis of the p62 complex, an assembly of O-linked glycoproteins that localizes near the central gated channel of the nuclear pore complex. Mol. Biol. Cell 6, 1591-1603.
26. Ho, S., Hunt, H.D., Morton, R.M., Pullen, J.K., and Pease, L.R. (1989). Site-directed mutagenesis by overlap extension using the polymerase chain reaction. Gene 77, 51-59.
27. Hu, T., Guan, T., and Gerace, L. (1996). Molecular and functional characterization of the p62 complex, an assembly of nuclear pore complex glycoproteins. J. Cell Biol. 134, 589-601.
28. Imamoto, N., Shimamoto, T., Kose, S., Takao, T., Tachibana, T., Matsubae, M., Sekimoto, T., Shimonishi, Y., and Yoneda, Y. (1995a). The nuclear pore-targeting complex binds to nuclear pores after association with a karyophile. FEBS Lett. 368, 415-419.
29. Imamoto, N., Shimamoto, T., Takao, T., Tachibana, T., Kose, S., Matsubae, M., Sekimoto, T., Shimonishi, Y., and Yoneda, Y. (1995b). In vivo evidence for involvement of a 58kDa component of nuclear pore-targeting complex in nuclear protein import. EMBO J. 14, 3617-3626.
30. Imamoto, N., Tachibana, T., Matsubae, M., and Yoneda, Y. (1995c). A karyophilic protein forms a stable complex with cytoplasmic components prior to nuclear pore binding. J. Biol. Chem. 270, 8559-8565.
31. Iovine, M.K., Watkins, J.L., and Wente, S.R. (1995). The GLFG repetitive region of the nucleoporin Nup116p interacts with Kap95p, an essential yeast nuclear import factor. J. Cell Biol. 131, 1699-1713.
32. Koepp, D.M., and Silver, P. (1996). A GTPase controlling nuclear trafficking: running the right way or walking randomly. Cell 87, 1-4.
33. Koepp, D.M., Wong, D.H., Corbett, A.H., and Silver, P.A. (1996). Dynamic localization of the nuclear import receptor and its interactions with transport factors. J. Cell Biol. 133, 1163-1176.
34. Lounsbury, K.M., Richards, S.A., Perlungher, R.R., and Macara, I.G. (1996). Ran binding domains promote the interaction of Ran with p97/β-karyopherin, linking the docking and translocation steps of nuclear import. J. Biol. Chem. 271, 2357-2360.
35. Mahajan, R., Delphin, C., Guan, T., Gerace, L., and Melchior, F. (1997). A small ubiquitin-related polypeptide involved in targeting RanGAP1 to nuclear pore complex protein RanBP2. Cell 88, 97-107.
36. Matunis, M.J., Coutavas, E., and Blobel, G. (1996). A novel ubiquitin-like modification modulates the partitioning of the Ran-GTPase-activating protein RanGAP1 between the cytosol and the nuclear pore complex. J. Cell. Biol. 135, 1457-1470.
37. Melchior, F., Guan, T., Yokoyama, N., Nishimoto, T., and Gerace, L. (1995). GTP hydrolysis by Ran occurs at the nuclear pore complex in an early step of protein import. J. Cell Biol. 131, 571-581.
38. Melchior, F.B., Paschal, B., Evans, J., and Gerace, L. (1993). Inhibition of nuclear protein import by nonhydrolyzable analogues of GTP and identification of the small GTPase Ran/TC4 as an essential transport factor. J. Cell Biol. 123, 1649-1659.
39. Moore, M.S., and Blobel, G. (1993). The GTP-binding protein Ran/ TC4 is required for protein import into the nucleus. Nature 365, 661-663.
40. Moore, M.S., and Blobel, G. (1994). Purification of a Ran-interacting protein that is required for protein import into the nucleus. Proc. Natl. Acad. Sci. USA 91, 10212-10216.
41. Moroianu, J., Blobel, G., and Radu, A. (1995a). Previously identified protein of uncertain function is karyopherin α and together with karyopherin β docks import substrate at nuclear pore complexes. Proc. Natl. Acad. Sci. USA 92, 2008-2011.
42. Moroianu, J., Blobel, G., and Radu, A. (1996). Nuclear protein import: Ran-GTP dissociates the karyopherin αβ heterodimer by displacing α from an overlapping binding site on β. Proc. Natl. Acad. Sci. USA 93, 7059-7062.
43. 2 subunit binds nuclear localization signal and β subunit interacts with peptide repeat-containing nucleoporins. Proc. Natl. Acad. Sci. USA 92, 6532-6536.
44. Nehrbass, U., and Blobel, G. (1996). Role of the nuclear transport factor p10 in nuclear import. Science 272, 120-122.
45. Newmeyer, D.D., and Forbes, D.J. (1990). An N-ethylmaleimide-sensitive cytosolic factor necessary for nuclear protein import: requirement in signal-mediated binding to the nuclear pore. J. Cell. Biol. 110, 547-557.
46. Panté, N., and Aebi, U. (1996). Sequential binding of import ligands to distinct nucleopore regions during their nuclear import. Science 273, 1729-1732.
47. Paschal, B.M., Delphin, C., and Gerace, L. (1996). Nucleotide-specific interaction of Ran/TC4 with nuclear transport factors NTF2 and p97. Proc. Natl. Acad. Sci. USA 93, 7678-7683.
48. Paschal, B.M., and Gerace, L. (1995). Identification of NTF2, a cytosolic factor for nuclear import that interacts with nuclear pore complex protein p62. J. Cell. Biol. 129, 925-937.
49. Pollard, V.W., Michael, W.M., Nakeilny, S., Siomi, M.C., Wang, F., and Dreyfuss, G. (1996). A novel receptor-mediated nuclear protein import pathway. Cell 86, 985-994.
50. Prieve, M.G., Guttridge, K.L., Mungia, J.E., and Waterman, M.L. (1996). The nuclear localization signal of lymphoid enhancer factor-1 is recognized by two differentially expressed Srp1-nuclear localization sequence receptor proteins. J. Biol. Chem. 271, 7654-7658.
51. Radu, A., Blobel, G., and Moore, M.S. (1995a). Identification of a protein complex that is required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins. Proc. Natl. Acad. Sci. USA 92, 1769-1773.
52. Radu, A., Moore, M.S., and Blobel, G. (1995b). The peptide repeat domain of nucleoporin Nup98 functions as a docking site in transport across the nuclear pore complex. Cell 81, 215-222.
53. Rexach, M., and Blobel, G. (1995). Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors, and nucleoporins. Cell 83, 683-692.
54. Saitoh, H., Cooke, C.A., Burgess, W.H., Earnshaw, W.C., and Dasso, M. (1996). Direct and indirect association of the small GTPase Ran with nuclear pore proteins and soluble transport factors: studies in Xenopus laevis egg extracts. Mol. Biol. Cell 7, 1319-1334.
55. Scheffzek, K., Klebe, C., K. Fritz-Wolf, Kabsch, W., and Wittnghofer, A. (1995). Crystal structure of the nuclear Ras-related protein Ran in its GDP-bound form. Nature 374, 378-381.
56. + RNA in the cytoplasm. Proc. Natl. Acad. Sci. USA 92, 225-229.
57. Siomi, H., and Dreyfuss, G. (1995). A nuclear localization domain in the hnRNP A1 protein. J. Cell Biol. 129, 551-559.
58. Tachibana, T., Imamoto, N., Seino, H., Nishimoto, T., and Yoneda, Y. (1994). Loss of RCC1 leads to suppression of nuclear protein import in living cells. J. Biol. Chem. 269, 24542-24545.
59. Weis, K., Mattaj, I.W., and Lamond, A.I. (1995). Identification of hSRP1a as a functional receptor for nuclear localization sequences. Science 268, 1049-1053.
60. Weis, K., Ryder, U., and Lamond, A. (1996). The conserved amino-terminal domain of hSRP1a is essential for nuclear protein import. EMBO J. 15, 1818-1825.
61. Wu J., Matunis, M.J., Kraemer, D., Blobel, G., and Coutavas, E. (1995). Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region. J. Biol. Chem. 270, 14209-14213.
62. Yano, R., Oakes, M.L., Yamagishi, M., Dodd, J.A., and Nomura, M. (1992). Cloning and characterization of SRP1, a suppressor of temperature-sensitive RNA polymerase I mutations, in Saccharomyces cerevisiae. Mol. Cell. Biol. 12, 5640-5651.
63. Yokoyama N., Hayashi, N., Seki, T., Panté, N., Ohba, T., Nishii, K., Kuma, K., Hayashida, T., Miyata, T., Aebi, U., Fukui, M., and Nishimoto, T. (1995). A giant nucleopore protein that binds Ran/ TC4. Nature 376, 184-188.