Evidence using a green fluorescent protein-glucocorticoid receptor chimera that the RAN/TC4 GTPase mediates an essential function independent of nuclear protein import

Journal of Cell Biology

Volumn 133, Issue 5, 1996, Pages 985-996

  Carey, Kimberly L ^a   Richards, Stephanie A ^a   Lounsbury, Karen M ^a   Macara, Ian G ^a,b  

^a UNIVERSITY OF VERMONT   (United States)

^b UNIVERSITY OF VERMONT COLLEGE OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

GLUCOCORTICOID RECEPTOR; GUANOSINE TRIPHOSPHATASE; NUCLEAR PROTEIN;

ANIMAL CELL; ARTICLE; CELL NUCLEUS MEMBRANE; CELL STRAIN BHK; CHIMERA; CONTROLLED STUDY; INTRACELLULAR TRANSPORT; NONHUMAN; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; PROTEIN TRANSPORT; TRANSPORT KINETICS;

AMINO ACID SEQUENCE; ANIMALS; BIOLOGICAL TRANSPORT, ACTIVE; CELL DIVISION; CELL LINE; CELL NUCLEUS; CRICETINAE; ESCHERICHIA COLI; GENE EXPRESSION; GREEN FLUORESCENT PROTEINS; GTP PHOSPHOHYDROLASES; LUMINESCENT PROTEINS; MICROINJECTIONS; MOLECULAR SEQUENCE DATA; MUTATION; NUCLEAR PROTEINS; RAN GTP-BINDING PROTEIN; RECEPTORS, GLUCOCORTICOID; RECOMBINANT FUSION PROTEINS; TRANSFECTION;

ANIMALIA; CRICETINAE; MAMMALIA;

EID: 0029899120     PISSN: 00219525     EISSN: None     Source Type: Journal    
DOI: 10.1083/jcb.133.5.985     Document Type: Article

References (41)

1. Adam, E., and S.A. Adam. 1994. Identification of cytosolic factors required for nuclear location sequence-mediated binding to the nuclear envelope. J. Cell Biol. 125:547-555.
2. Becker, J., F. Melchior, V. Gerke, F.R. Bischoff, H. Ponstingl, and A. Wittinghofer. 1995. RNA1 encodes a GTPase-activating protein specific for Gsp1. the Ran/TC4 homolog of Saccharomyces cerevisiae. J. Biol. Chem. 270: 11860-11865.
3. Beddow, A.L.,S.A. Richards, N.R. Orem, and I.G. Macara. 1995. The Ran/TC4 GTPase binding domain: identification by expression cloning and characterization of a novel sequence motif. Proc. Natl. Acad. Sci. USA. 92:3328-3332.
4. Bischoff, F.R., and H. Ponstingl. 1991. Mitotic regulator protein RCC1 is complexed with a nuclear ras-related polypeptide. Proc. Natl. Acad. Sci. USA. 88:10830-10834.
5. Bischoff, F.R., H. Krebber, T. Kempf, I. Hermes, and H. Ponstingl. 1995. Human RanGTPase-activating protein RanGAP1 is a homologue of yeast Rna1p involved in mRNA processing and transport. Proc. Natl. Acad. Sci. USA. 92:1749-1753.
6. Bloom, E., D.T. Matulich, N.C. Lan, S.J. Higgins, S.S. Simons, and J.D. Baxter. 1980. Nuclear binding of glucocorticoid receptors: relations between cytosol binding, activation and the biological response. J. Steroid Biochem. 12:175-184.
7. Bourne, H.R. 1988. Do GTPases direct membrane traffic in secretion? Cell. 53: 669-671.
8. Chalfie, M., Y. Tu, G. Euskirchen, W.W. Ward, and D.C. Prasher. 1994. Green fluorescent protein as a marker for gene expression. Science (Wash. DC). 263:802-805.
9. Cheng, Y., J.E. Dahlberg, and E. Lund. 1995. Diverse effects of the guanine nucleolide exchange factor RCC1 on RNA transport. Science (Wash. DC). 267: 1807-1810.
10. Corbett, A.M., D.M. Koepp, G. Schlenstedt, M.S. Lee, A.K. Hopper, and P.A. Silver. 1995. Rnalp, a Ran/TC4 GTPase activating protein, is required for nuclear import. J. Cell Biol. 130:1017-1026.
11. Cserpan, J., and A. Udvardy. 1995. The mechanism of nuclear transport of natural or artificial transport substrates in digitonin-permeabilized cells. J. Cell Sci. 108:1849-1861.
12. Dascher, C., and W.E. Balch. 1994. Dominant inhibitory mutants of ARF1 block endoplasmic reticulum to Golgi transport and trigger disassembly of the Golgi apparatus. J. Biol. Chem. 269:1437-1448.
13. Dasso, M., T. Seki, Y. Azuma, T. Ohba, and T. Nishimoto. 1994. A mutant form of the Ran/TC4 protein disrupts nuclear fusion in Xenopus laevis egg extracts by inhibiting the RCC1 protein, a regulator of chromosome condensation. EMBO (Eur. Mol. Biol. Organ.) J. 13:5732-5744.
14. Eckert, R.L., and B.S. Katzenellenbogen. 1982. Physical properties of estrogen receptor complexes in MCF-7 human breast cancer cells. J. Biol. Chem. 257: 8840-8846.
15. Gorlich, D.S., S. Prehn, R.A. Laskey, and E. Hartmann. 1994. Isolation of a protein that is essential for the first step of nuclear protein import. Cell. 79: 767-778.
16. Gorlich, D.S., F. Vogel, A.D. Mills, E. Hartmann, and R.A. Laskey. 1995. Distinct functions for two importin subunits in nuclear protein import. Nature (Land.). 377:246-248.
17. Heim, R., A.B. Cubitt, and R.Y. Tsien. 1995. Improved green fluorescence. Nature (Lond.). 373:663-664.
18. Kadowaki, T., D. Goldfarb, L.M. Spitz, A.M. Tarkatoff, and M. Ohno. 1993. Regulation of RNA processing and transport by a nuclear guanine nucleotide release protein and members of the Ras superfamily. EMBO (Eur. Mol. Biol. Organ.) J. 12:2929-2937.
19. rcc1 and fluorescence measurements of their interactions. Biochemistry. 32:11923-11928.
20. Kornbluth, S., M. Dasso, and J. Newport. 1994. Evidence for a dual role for TC4 protein in regulating nuclear structure and cell cycle progression. J. Cell Biol. 125:705-719.
21. Lounsbury, K.M., A.L. Beddow, and I.G. Macara. 1994. A family of proteins that stabilize the Ran/TC4 GTPase in its GTP-bound conformation. J. Biol. Chem. 269:11285-11290.
22. Lounsbury, K.M., S.A. Richards, R.R. Perlungher, and I.G. Macara. 1996. Ran binding domains promote the interaction of Ran with p97/β-karyopherin, linking the docking and translocation steps of nuclear import. J. Biol. Chem. 271:2357-2360.
23. Mattingly, R.R., A. Sorisky, M.R. Brann, and I.G. Macara. 1994. Muscarinic receptors transform NIH 3T3 cells through a Ras-dependent pathway inhibited by the Ras GTPase-activating protein SH3 domain. Mol. Cell. Biol. 14: 7943-7952.
24. Melchior, F., and L. Gerace. 1995. Mechanisms of nuclear protein import. Curr. Opin. Cell Biol. 7:310-318.
25. Melchior, F., B. Pashal, J. Evans, and L. Gerace. 1993. Inhibition of nuclear protein import by nonhydrolyzable analogs of GTP and identification of the small GTPase Ran/TC4 as an essential transport factor. J. Cell Biol. 123: 1649-1659.
26. Moore, M.S., and G. Blobel. 1993. The GTP-binding protein RanATC4 is required for protein import into the nucleus. Nature (Land.). 365:661-663.
27. Moroianu, J., and G. Blobel. 1995. Protein export from the nucleus requires the GTPase Ran and GTP hydrolysis. Proc. Natl. Acad. Sci. USA. 92:4318-4322.
28. Picard, D., and K. Yamamoto. 1987. Two signals mediate hormone-dependent nuclear localization of the glucocorticoid receptor. EMBO (Eur. Mol. Biol. Organ.) J. 6:3333-3340.
29. Qi, M., B.J. Hamilton, and D. DeFranco. 1989. V-mos oncoproteins affect the nuclear retention and reutilizalion of glucocorticoid receptors. Mol. Endocrinol. 3:1279-1288.
30. Radu, A., G. Blobel, and M.S. Moore. 1995. Identification of a protein complex that is required for nuclear protein import and mediates docking of import substrate to distinct nucleoporins. Proc. Natl. Acad. Sci. USA. 92:1769-1773.
31. Ren, M., A. Villamarin, A. Shih, E. Coutavas, M.S. Moore, M. Locurcio. V. Clarke, J.D. Oppenheim, P. D'Eustachio, and M.G. Rush. 1995. Separate domains of the Ran GTPase interact with different factors to regulate nuclear protein import and RNA processing. Mol. Cell. Biol. 15:2117-2124.
32. Ren, M., E. Coutavas, P. D'Eustachio, and M.G. Rush. 1994. Effects of mutant Ran/TC4 proteins on cell cycle progression. Mol. Cell. Biol. 14:4216-4224.
33. Rexach, M., and G. Blobel. 1995. Protein import into nuclei: association and dissociation reactions involving transport substrate, transport factors and nucleoporins. Cell. 83:683-692.
34. Richards, S.A., K.M. Lounsbury. and I.G. Macara. 1995. The C-terminus of the nuclear Ran/TC4 GTPase stabilizes the GDP-bound state and mediates interactions with RCC1. Ran-GAP and HTF9A/RanBP1. J. Biol. Chem. 270: 14405-14411.
35. Sambrook, J., E.F. Fritsch, and T. Maniatis. 1989. Molecular Cloning: A Laboratory Manual. 2nd edition. Vol. 3. CHS Press, Plainview, NY.
36. Satoh, T., M. Nakafuka, and Y. Kaziro. 1992. Function of Ras as a molecular switch in signal transduction. J. Biol. Chem. 267:24149-24152.
37. Sazer, S., and P. Nurse. 1994. A fission yeast RCC1-related protein is required for the mitosis to interphase transition. EMBO (Eur. Mol. Biol. Organ.) J. 13:606-615.
38. Sheffzek, K., C. Klebe, K. Fritz-Wolf, W. Kahsch, and A. Wittinghofer. 1995. Crystal structure of the nuclear Ras-related protein Ran in its GDP-bound form. Nature (Lond.). 374:378-381.
39. Schlenstcdt, G., C. Saavedra, J.D.J. Loeb, C.N. Cole, and P.A. Silver. 1995. The GTP-bound form of the yeast Ran/TC4 homologue blocks nuclear protein import and appearance of poly(A)+ RNA in the cytoplasm. Proc. Natl. Acad. Sci. USA. 92:225-229.
40. Wu, J., M.I. Matunis, D. Kraemer, G. Blobel, and E. Coutavas. 1995. Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a Cyclophilin A homologous domain, and a leucinerich region. J. Biol. Chem. 270:14209-14213.
41. Yokoyama. N., N. Hayashi, T. Seki, N. Pante, T. Ohba, K. Nishii, K. Kuma, T. Hayashida, T. Miyata, U. Aebi, et al. 1995. A giant nucleopore protein that binds Ran/TC4. Nature (Lond). 376:184-188.