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G. Baggermann, A. Cerstiaens, A. De Loof, and L. Schoofs Peptidomics of the larval Drosophila melanogaster central nervous system J Biol Chem 277 2002 40368 40374 This paper convincingly demonstrates how efficient modern MS can be applied in terms of sensitivity and sequence elucidation. A total of 28 neuropeptides were identified from only 50 larval Drosophila, whereas classical approaches required several 100 000 larvae.
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M. Ramström, and J. Bergquist Miniaturized proteomics and peptidomics using capillary liquid separation and high resolution mass spectrometry FEBS Lett 567 2004 92 95
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T. Kaiser, H. Kamal, A. Rank, H.J. Kolb, E. Holler, A. Ganser, B. Hertenstein, H. Mischak, and E.M. Weissinger Proteomics applied to the clinical follow-up of patients after allogeneic hematopoietic stem cell transplantation Blood 104 2004 340 349 An example of a sophisticated analytical procedure and its application in diagnostics. CE-MS instrumentation combined with bioinformatics has added a new tool to a specific clinical field. A closely related application of this technique is described in [50].
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Mischak H, Kaiser T, Walden M, Hillmann M, Wittke S, Herrmann A, Knueppel S, Haller H, Fliser D: Proteomic analysis for assessment of diabetic renal damage in humans. Clin Sci (Lond) 2004, doi:10.1042/CS20040103.
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P. Lewczuk, H. Esselmann, T.W. Groemer, M. Bibl, J.M. Maler, P. Steinacker, M. Otto, J. Kornhuber, and J. Wiltfang Amyloid β peptides in cerebrospinal fluid as profiled with surface enhanced laser desorption/ ionization time-of-flight mass spectrometry: evidence of novel biomarkers in Alzheimer's disease Biol Psychiatry 55 2004 524 530
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Peptidomics-based approach reveals the secretion of the 29-residue COOH-terminal fragment of the putative tumor suppressor protein DMBT1 from pancreatic adenocarcinoma cell lines
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K. Sasaki, K. Sato, Y. Akiyama, K. Yanagihara, M. Oka, and K. Yamaguchi Peptidomics-based approach reveals the secretion of the 29-residue COOH-terminal fragment of the putative tumor suppressor protein DMBT1 from pancreatic adenocarcinoma cell lines Cancer Res 62 2002 4894 4898 Example of the use of SELDI technology to detect potential cancer-related biomarkers. Combination of a less complex test mixture of cultured cell supernatants with selective on-target sample preparation represents a promising application for SELDI-MS broadening its diagnostic value.
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