Isolation and biochemical characterization of LEAP-2, a novel blood peptide expressed in the liver

Protein Science

Volumn 12, Issue 1, 2003, Pages 143-152

  Krause, Alexander ^a   Sillard, Rannar ^b   Kleemeier, Burkhard ^a   Klüver, Enno ^a   Maronde, Erik ^a   Conejo García, José Ramon ^a   Forssmann, Wolf Georg ^a   Schulz Knappe, Peter ^c   Nehls, Michael C ^d   Wattler, Frank ^d   Wattler, Sigrid ^d   Adermann, Knut ^a  

^a IPF PHARMACEUTICALS GMBH   (Germany)

^b KAROLINSKA INSTITUTE   (Sweden)

^c BioVisioN AG   (Germany)

^d Ingenium Pharmaceuticals AG   (Germany)

Author keywords

Alternative splicing; Antimicrobial activity; Disulfide bonds; Hemofiltrate; Liver; Peptide; Secretion

Indexed keywords

ANTIINFECTIVE AGENT; COMPLEMENTARY DNA; PEPTIDE; PROTEIN LEAP 2; RNA; UNCLASSIFIED DRUG;

ALTERNATIVE RNA SPLICING; ANTIMICROBIAL ACTIVITY; ARTICLE; CHROMOSOMAL LOCALIZATION; DISULFIDE BOND; DRUG STRUCTURE; HUMAN; LIVER; MASS SPECTROMETRY; NONHUMAN; NUCLEOTIDE SEQUENCE; PEPTIDE ANALYSIS; PRIORITY JOURNAL; PROMOTER REGION; TISSUE DISTRIBUTION;

ALTERNATIVE SPLICING; AMINO ACID SEQUENCE; ANIMALS; ANTI-INFECTIVE AGENTS; ANTIMICROBIAL CATIONIC PEPTIDES; BASE SEQUENCE; BLOOD PROTEINS; CLONING, MOLECULAR; DISULFIDES; DNA, COMPLEMENTARY; DOSE-RESPONSE RELATIONSHIP, DRUG; HEMOFILTRATION; HUMANS; LIVER; MICROBIAL SENSITIVITY TESTS; MOLECULAR SEQUENCE DATA; ORGAN SPECIFICITY; SACCHAROMYCES CEREVISIAE; SEQUENCE ALIGNMENT; SPECTROMETRY, MASS, ELECTROSPRAY IONIZATION;

MAMMALIA;

EID: 12244313876     PISSN: 09618368     EISSN: None     Source Type: Journal    
DOI: 10.1110/ps.0213603     Document Type: Article

References (30)

1. Adermann, K., Wattler, F., Wattler, S., Heine, G., Meyer, M., Forssmann, W.G., and Nehls, M. 1999. Structural and phylogenetic characterization of human SLURP-1, the first secreted mammalian member of the Ly-6/uPAR protein. Protein Sci. 8: 810-819.
2. Andreu, D. and Rivas, L. 1998. Animal antimicrobial peptides. An overview. Biopolymers (Peptide Science) 47: 415-433.
3. Ayoubi, T.A. and van de Ven, W.J. 1996. Regulation of gene expression by alternative promoters. FASEB J. 10: 453-460.
4. Bièche, I., Olivi, M., Champème, M.-H., Vidaud, D., Lidereau, R., and Vidaud, M. 1998. Novel approach to quantitative polymerase chain reaction using real-time detection: Application to the detection of gene amplification in cancer. Int. J. Cancer 78: 661-666.
5. Chen, J., Falla, T.J., Liu, H., Hurst, M.A., Fujii, C.A., Mosca, D.A., Embree, J.R., Loury, D.J., Radel, P.A., Cheng Chang, C., et al. 2000. Development of protegrins for the treatment and prevention of oral mucositis: Structure-activity relationships of synthetic protegrin analogues. Biopolymers (Peptide Science) 55: 88-98.
6. Detheux, M., Ständker, L., Vakili, J., Münch, J., Forssmann, U., Adermann, K., Pöhlmann, S., Vassart, G., Kirchhoff, F., Parmentier, M., et al. 2000. Natural proteolytic processing of hemofiltrate CC chemokine 1 generates a potent CC chemokine receptor (CCR)1 and CCR5 with anti-HIV properties. J. Exp. Med. 192: 1501-1508.
7. Ganz, T. 1999. Defensins and host defense. Science 286: 420.
8. Harrison, P.M., Kumar, A., Lang, N., Snyder, M., and Gerstein, M. 2002. A question of size: The eukaryotic proteome and the problems in defining it. Nucleic Acids Res. 30: 1083-1090.
9. John, H., Preissner, K.T., Forssmann, W.G., and Ständker, L. 1999. Novel glycosylated forms of human plasma endostatin and circulating endostatin-related fragments of collagen XV. Biochemistry 38: 10217-10224.
10. Kozak, M. 1991. An analysis of vertebrate mRNA sequences: Intimations of translational control. J. Cell Biol. 115: 887-903.
11. -, 1996. Interpreting cDNA sequences: Some insights from studies on translation. Mamm. Genome 7: 563-574.
12. Krause, A., Neitz, S., Mägert, H.J., Schulz, A., Forssmann, W.G., Schulz-Knappe, P., and Adermann, K. 2000. LEAP-1, a novel highly disulfide-bonded human peptide, exhibits antimicrobial activity. FEBS Lett. 480: 147-150.
13. Lander, E.S., Linton, L.M., Birren, B., Nusbaum, C., Zody, M.C., Baldwin, J., Devon, K., Dewar, K., Doyle, M., FitzHugh, W., et al. 2001. International Human Genome Sequencing Consortium. Initial sequencing and analysis of the human genome. Nature 409: 860-921.
14. Lehrer, R.I., Rosenman, M., Harwig, S.S.S.L., Jackson, R., and Eisenhauer, P. 1991. Ultrasensitive assays for endogenous antimicrobial polypeptides. J. Immunol. Methods 137: 167-173.
15. Mägert, H.J., Reinecke, M., David, I., Raab, H.R., Adermann, K., Zucht, H.D., Hill, O., Hess, R., and Forssmann, W.G. 1998. Uroguanylin: Gene structure, expression, processing as a peptide hormone, and co-storage with somatostatin in gastrointestinal D-cells. Regul. Pept. 73: 165-176.
16. Mägert, H.J., Ständker, L., Kreutzmann, P., Zucht, H.D., Reinecke, M., Sommerhoff, C.P., Fritz, H., and Forssmann, W.G. 1999. LEKTI, A novel 15-domain type of human serine proteinase inhibitor. J. Biol. Chem. 274: 21499-21502.
17. Motzkus, D., Maronde, E., Grunenberg, U., Lee, C.C., Forssmann, W.G., and Albrecht, U. 2000. The human PER1 gene is transcriptionally regulated by multiple signaling pathways. FEBS Lett. 486: 315-319.
18. Nakayama, K. 1997. Furin: A mammalian subtilisin/Kex2p-like endoprotease involved in processing of a wide variety of precursor proteins. Biochem. J. 327: 625-635.
19. Nicolas, G., Bennoun, M., Devaux, I., Beaumont, C., Grandchamp, B., Kahn, A., and Vaulont, S. 2001. Lack of hepcidin gene expression and severe tissue iron overload in upstream stimulatory factor 2 (USF2) knockout mice. Proc. Natl. Acad. Sci. 98: 8780-8785.
20. Nielsen, H., Engelbrecht, J., Brunak, S., and von Heijne, G. 1997. Identification of prokaryotic and eukaryotic signal peptides and prediction of their cleavage sites. Protein Eng. 10: 1-6.
21. Park, C.H., Valore, E.V., Waring, A.J., and Ganz, T. 2001. Hepcidin: A urinary antimicrobial peptide synthesized in the liver. J. Biol. Chem. 276: 7806-7810.
22. Pigeon, C., Ilyin, G., Courselaud, B., Leroyer, P., Turlin, B., Brissot, P., and Loreal, O. 2001. A new mouse liver-specific gene, encoding a protein homologous to human antimicrobial peptide hepcidin, is overexpressed during iron overload. J. Biol. Chem. 276: 7811-7819.
23. Rappsilber, J. and Mann, M. 2002. What does it mean to identify a protein in proteomics? Trends Biochem. Sci. 27: 74-78.
24. Schulz-Knappe, P., Schrader, M., Ständker, L., Richter, R., Hess, R., Jürgens, M., and Forssmann, W.G. 1997. Peptide bank generated by large-scale preparation of circulating human peptides. J. Chromatogr. A 776: 125-132.
25. Sillard, R., Jornvall, H., Carlquist, M., and Mutt, V. 1993. Chemical assay for cyst(e)ine-rich peptides detects a novel intestinal peptide ZF-1, homologous to a single zinc-finger motif. Eur. J. Biochem. 211: 377-380.
26. Venter, J.C., Adams, M.D., Myers, E.W., Li, P.W., Mural, R.J., Sutton, G.G., Smith, H.O., Yandell, M., Evans, C.A., Holt, R.A., et al. 2001. The sequence of the human genome. Science 291: 1304-1351.
27. Wenderfer, S.E., Slack, J.P., McCluskey, T.S., and Monaco, J.J. 2000. Identification of 40 genes on a 1-Mb contig around the IL-4 cytokine family gene cluster on mouse chromosome 11. Genomics 63: 354-373.
28. 2-terminal heterogeneity. Eur. J. Biochem. 260: 421-429.
29. Zadina, J.E., Hackler, L., Ge, L.J., and Kastin, A.J. 1997. A potent and selective endogenous agonist for the mu-opiate receptor. Nature 386: 499-502.
30. Zucht, H.D., Grabowsky, J., Schrader, M., Liepke, C., Jürgens, M., Schulz-Knappe, P., and Forssmann, W.G. 1998. Human β-defensin-1: A urinary peptide present in variant molecular forms and its putative functional implication. Eur. J. Med. Res. 3: 315-323.