Improved molecular diagnosis of COVID-19 by the novel, highly sensitive and specific COVID-19-RdRp/Hel real-time reverse transcription-PCR assay validated in vitro and with clinical specimens

Journal of Clinical Microbiology

Volumn 58, Issue 5, 2020, Pages

  Chan, Jasper Fuk Woo ^a,b,c,d,e   Yip, Cyril Chik Yan ^f   To, Kelvin Kai Wang ^a,b,c   Tang, Tommy Hing Cheung ^g   Wong, Sally Cheuk Ying ^g   Leung, Kit Hang ^c   Fung, Agnes Yim Fong ^c   Ng, Anthony Chin Ki ^c   Zou, Zijiao ^c   Tsoi, Hoi Wah ^c   Choi, Garnet Kwan Yue ^f   Tam, Anthony Raymond ^f   Cheng, Vincent Chi Chung ^f   Chan, Kwok Hung ^a,c   Tsan, Owen Tak Yin ^g,h   Yuen, Kwok Yung ^b,c,d,e  

^a THE UNIVERSITY OF HONG KONG   (Hong Kong)

^b THE UNIVERSITY OF HONG KONG SHENZHEN HOSPITAL   (China)

^c UNIVERSITY OF HONG KONG   (Hong Kong)

^d HAINAN MEDICAL UNIVERSITY   (China)

^e UNIVERSITY OF HONG KONG   (Hong Kong)

^f QUEEN MARY HOSPITAL   (Hong Kong)

^g QUEEN ELIZABETH HOSPITAL   (Hong Kong)

^h PRINCESS MARGARET HOSPITAL   (Hong Kong)

Author keywords

Coronavirus; COVID 19; Diagnostic; Emerging; PCR; SARS; Virus; Wuhan

Indexed keywords

GENOMIC RNA; NUCLEOCAPSID PROTEIN; RNA DIRECTED RNA POLYMERASE; RNA HELICASE; VIRUS SPIKE PROTEIN; VIRAL PROTEIN;

ADULT; AGED; CELL CULTURE; CHINA; CLINICAL ARTICLE; CORONAVIRUS DISEASE 2019; CROSS REACTION; DIAGNOSTIC TEST ACCURACY STUDY; EPIDEMIC; FEMALE; HONG KONG; HUMAN; IN VITRO STUDY; INTERMETHOD COMPARISON; LABORATORY DIAGNOSIS; LIMIT OF DETECTION; MALE; MOLECULAR DIAGNOSIS; PRIORITY JOURNAL; REAL TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; REVIEW; SENSITIVITY AND SPECIFICITY; SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS 2; VALIDATION STUDY; VIRUS DETECTION; VIRUS GENE; VIRUS LOAD; VIRUS PNEUMONIA; ANIMAL; BETACORONAVIRUS; CHLOROCEBUS AETHIOPS; CORONAVIRUS INFECTION; GENETICS; ISOLATION AND PURIFICATION; MIDDLE AGED; PANDEMIC; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VERO CELL LINE; VIROLOGY;

ADULT; AGED; ANIMALS; BETACORONAVIRUS; CHINA; CHLOROCEBUS AETHIOPS; CORONAVIRUS INFECTIONS; FEMALE; HUMANS; IN VITRO TECHNIQUES; MALE; MIDDLE AGED; MOLECULAR DIAGNOSTIC TECHNIQUES; PANDEMICS; PNEUMONIA, VIRAL; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VERO CELLS; VIRAL PROTEINS;

EID: 85083226778     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.00310-20     Document Type: Review

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