Respiratory tract samples, viral load, and genome fraction yield in patients with middle east respiratory syndrome

Journal of Infectious Diseases

Volumn 210, Issue 10, 2014, Pages 1590-1594

  Memish, Ziad A ^a   Al Tawfiq, Jaffar A ^d,g   Makhdoom, Hatem Q ^a,f   Assiri, Abdullah ^a   Alhakeem, Raafat F ^a   Albarrak, Ali ^b   Alsubaie, Sarah ^c   Al Rabeeah, Abdullah A ^a   Hajomar, Waleed H ^a,e,f   Hussain, Raheela ^a,f   Kheyami, Ali M ^a,f   Almutairi, Abdullah ^a,f   Azhar, Esam I ^a   Drosten, Christian ^h   Watson, Simon J ⁱ   Kellam, Paul ⁱ   Cotton, Matthew ⁱ   Zumla, Alimuddin ^a,i  

^a MINISTRY OF HEALTH   (Saudi Arabia)

^b The General Directorate of Armed Forces Health Services   (Saudi Arabia)

^c KING SAUD UNIVERSITY   (Saudi Arabia)

^d DHAHRAN HEALTH CENTER   (Saudi Arabia)

^e KING ABDULAZIZ UNIVERSITY   (Saudi Arabia)

^f INDIANA UNIVERSITY SCHOOL OF MEDICINE   (United States)

^g UNIVERSITY HOSPITAL BONN   (Germany)

^h WELLCOME TRUST SANGER INSTITUTE   (United Kingdom)

ⁱ UNIVERSITY COLLEGE LONDON HOSPITALS   (United Kingdom)

Author keywords

clinical; coronavirus; Ct value; diagnosis; genome fraction; MERS CoV; Middle East; molecular; RT PCR; screening; viral load

Indexed keywords

ARTICLE; GENOME ANALYSIS; HUMAN; LUNG LAVAGE; MAJOR CLINICAL STUDY; MIDDLE EAST; NASOPHARNGEAL SWAB; NONHUMAN; REAL TIME POLYMERASE CHAIN REACTION; SARS CORONAVIRUS; SMEAR; SPUTUM SMEAR; TRACHEAL ASPIRATION PROCEDURE; VIRUS DETECTION; VIRUS GENOME; VIRUS LOAD; CORONAVIRUS INFECTION; ISOLATION AND PURIFICATION; MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS; PATHOLOGY; POLYMERASE CHAIN REACTION; RESPIRATORY SYSTEM; VIROLOGY;

CORONAVIRUS INFECTIONS; HUMANS; MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS; POLYMERASE CHAIN REACTION; RESPIRATORY SYSTEM; VIRAL LOAD;

EID: 84901422841     PISSN: 00221899     EISSN: 15376613     Source Type: Journal    
DOI: 10.1093/infdis/jiu292     Document Type: Article

References (25)

1. Lieberman D, Shimoni A, Keren-Naus R, et al. Identification of respiratory viruses in adults: nasopharyngeal versus oropharyngeal sampling. J Clin Microbiol 2009; 47:3439-43.
2. Loens K, Van Heirstraeten L, Malhotra-Kumar S, et al. Optimal sampling sites and methods for detection of pathogens possibly causing community-Acquired lower respiratory tract infections. J Clin Microbiol 2009; 47:21-31.
3. Rello JA, Rodrguez P, Ibaez L, et al. Intensive care adult patients with severe respiratory failure caused by influenza A (H1N1)v in Spain. Crit Care 2009; 13:R148.
4. To KK, Chan KH, Li IW, et al. Viral load in patients infected with pandemic H1N1 2009 influenza A virus. J Med Virol 2010; 8:1-7.
5. Hung IFN, Lau SKP, Woo PCY, et al. Viral loads in clinical specimens and SARS manifestations. Hong Kong Med J 2009; 15:S20-2.
6. Liu CL, Lu YT, Peng MJ, et al. Clinical and laboratory features of severe acute respiratory syndrome vis-A-vis onset of fever. Chest 2004; 126:509-17.
7. Fan CK, Yieh KM, Peng MY, et al. Clinical and laboratory features in the early stage of severe acute respiratory syndrome. J Microbiol Immunol Infect 2006; 39:45-53.
8. Chan PKS, To W-K, Ng K-C, et al. Laboratory diagnosis of SARS. Emerg Infect Dis 2004; 10:825-31.
9. Hui DS, Chan PK. Severe acute respiratory syndrome and coronavirus. Infect Dis Clin North Am 2010; 3:619-38.
10. Rainer TH, Lee N, Ip M, et al. Features discriminating SARS from other severe viral respiratory tract infections. Eur J Clin Microbiol Infect Dis 2007; 26:121-9.
11. Zaki AM, van Boheemen S, Bestebroer TM, et al. Isolation of a novel coronavirus from a man with pneumonia in Saudi Arabia. N Engl J Med 2012; 367:1814-20.
12. Assiri A, Al-Tawfiq JA, Al-Rabeeah AA, et al. Epidemiological, demographic, and clinical characteristics of 47 cases of Middle East respiratory syndrome coronavirus disease from Saudi Arabia: a descriptive study. Lancet Infect Dis 2013; 9:752-61.
13. Assiri A, McGeer A, Perl TM, et al. Hospital outbreak of Middle East respiratory syndrome coronavirus. N Engl J Med 2013; 369:407-16.
14. Bermingham A, Chand MA, Brown CS, et al. Severe respiratory illness caused by a novel coronavirus, in a patient transferred to the United Kingdom from the Middle East, September 2012. Euro Surveill 2012; 17:20290.
15. Mailles A, Blanckaert K, Chaud P, et al. First cases of Middle East respiratory syndrome coronavirus (MERS-CoV) infections in France, investigations and implications for the prevention of humanto- human transmission, France, May 2013. Euro Surveill 2013; 18: 20502.
16. Guery B, Poissy J, El Mansouf L, et al. Clinical features and viral diagnosis of two cases of infection with Middle East Respiratory Syndrome coronavirus: a report of nosocomial transmission. Lancet 2013; 381: 2265-74.
17. Buchholz U, Mller MA, Nitsche A, et al. Contact investigation of a case of human novel coronavirus infection treated in a German hospital, October- November 2012. Euro Surveill 2013; 18:20406.
18. Drosten C, Seilmaier M, Corman VM, et al. Clinical features and virological analysis of a case of Middle East respiratory syndrome coronavirus infection. Lancet Infect Dis 2013; 13: 13:745-51.
19. Memish ZA, Al-Tawfiq JA, Makhdoom HQ, et al. Screening for Middle East respiratory syndrome coronavirus infection in hospital patients and their healthcare worker and family contacts: a prospective descriptive study. Clin Microbiol Infect; 2014. doi:10.1111/1469-0691.12562. [Epub ahead of print]. PMID: 24460984.
20. Corman VM, Eckerle I, Bleicker T, et al. Detection of a novel human coronavirus by real-time reverse-transcription polymerase chain reaction. Euro Surveill 2012; 17:20285.
21. Corman VM, Muller MA, Costabel U, et al. Assay for laboratory confirmation of novel human coronavirus (hCoV-EMC) infections. Euro Surveill 2012; 17:20334.
22. Cotten M,Watson SJ, Kellam P, et al. Transmission and evolution of the Middle East respiratory syndrome coronavirus in Saudi Arabia: a descriptive genomic study. Lancet 2013; 382:1993-2002.
23. Cotten M,Watson SJ, Zumla AI, et al. Spread, circulation, and evolution of the Middle East respiratory syndrome coronavirus. MBio 2014; 5: e01062-13.
24. Memish ZA, Cotten M, Meyer B, et al. Human infection with MERS Cornavirus after exposure to infected camels, Saudi Arabia. Emerg Infect Dis 2014; 20:1012-5.
25. Raj VS, Mou H, Smits SL, et al. Dipeptidyl peptidase 4 is a functional receptor for the emerging human coronavirus-EMC. Nature 2013; 495:251-4.