Development and evaluation of novel real-time reverse transcription-PCR assays with locked nucleic acid probes targeting leader sequences of human-pathogenic coronaviruses

Journal of Clinical Microbiology

Volumn 53, Issue 8, 2015, Pages 2722-2726

  Chan, Jasper Fuk Woo ^a,b   Choi, Garnet Kwan Yue ^b   Tsang, Alan Ka Lun ^b   Tee, Kah Meng ^b   Lam, Ho Yin ^c   Yip, Cyril Chik Yan ^b   To, Kelvin Kai Wang ^a,b   Cheng, Vincent Chi Chung ^b   Yeung, Man Lung ^b   Lau, Susanna Kar Pui ^a,b   Woo, Patrick Chiu Yat ^a,b   Chan, Kwok Hung ^b   Tang, Bone Siu Fai ^c   Yuen, Kwok Yung ^a,b  

^a THE UNIVERSITY OF HONG KONG   (Hong Kong)

^b UNIVERSITY OF HONG KONG   (Hong Kong)

^c HONG KONG SANATORIUM AND HOSPITAL   (Hong Kong)

Author keywords

[No Author keywords available]

Indexed keywords

LOCKED NUCLEIC ACID; VIRUS RNA; OLIGONUCLEOTIDE; OLIGONUCLEOTIDE PROBE;

5' UNTRANSLATED REGION; ADOLESCENT; ADULT; AGED; ANALYTIC METHOD; ARTICLE; CHILD; CLINICAL EVALUATION; CONTROLLED STUDY; CORONAVIRIDAE; FEMALE; HUMAN; HUMAN CORONAVIRUS 229E LS; HUMAN CORONAVIRUS HKU1 LS; HUMAN CORONAVIRUS NL63 LS; HUMAN CORONAVIRUS OC43 LS; INTERMETHOD COMPARISON; LIMIT OF DETECTION; MAJOR CLINICAL STUDY; MALE; MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS; MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS LS; MULTIPLEX POLYMERASE CHAIN REACTION; NASOPHARYNGEAL ASPIRATION; NONHUMAN; NUCLEIC ACID PROBE; PRIORITY JOURNAL; REAL TIME POLYMERASE CHAIN REACTION; REAL TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RESPIRATORY VIRUS; RESPLEX II; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SEQUENCE ANALYSIS; VERY ELDERLY; VIRUS CULTURE; VIRUS DETECTION; VIRUS ISOLATION; VIRUS STRAIN; ANIMAL; CLASSIFICATION; COMPARATIVE STUDY; CORONAVIRUS INFECTIONS; EVALUATION STUDY; GENETICS; INFANT; ISOLATION AND PURIFICATION; MIDDLE AGED; OLIGONUCLEOTIDE PROBE; PRESCHOOL CHILD; PROCEDURES; VIROLOGY; YOUNG ADULT;

CORONAVIRUS;

ADOLESCENT; ADULT; AGED; AGED, 80 AND OVER; ANIMALS; CHILD; CHILD, PRESCHOOL; CORONAVIRUS; CORONAVIRUS INFECTIONS; FEMALE; HUMANS; INFANT; MALE; MIDDLE AGED; OLIGONUCLEOTIDE PROBES; OLIGONUCLEOTIDES; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; YOUNG ADULT;

EID: 84938100010     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01224-15     Document Type: Article

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