RNA sequencing by direct tagmentation of RNA/DNA hybrids

Proceedings of the National Academy of Sciences of the United States of America

Volumn 117, Issue 6, 2020, Pages 2886-2893

  Di, Lin ^a   Fu, Yusi ^a,i   Sun, Yue ^a   Li, Jie ^b   Liu, Lu ^b   Yao, Jiacheng ^b   Wang, Guanbo ^c,d   Wu, Yalei ^e   Lao, Kaiqin ^e   Lee, Raymond W ^e   Zheng, Genhua ^e   Xu, Jun ^f   Oh, Juntaek ^f   Wang, Dong ^f   Sunney Xie, X ^a   Huang, Yanyi ^a,d,g,h   Wang, Jianbin ^b,h  

^a PEKING UNIVERSITY   (China)

^b TSINGHUA UNIVERSITY   (China)

^c NANJING NORMAL UNIVERSITY   (China)

^d Shenzhen Bay Laboratory ^*  (China)

^e XGen US Co   (United States)

^f UNIVERSITY OF CALIFORNIA   (United States)

^g PEKING UNIVERSITY   (China)

^h CHINESE INSTITUTE FOR BRAIN RESEARCH   (China)

ⁱ BAYLOR COLLEGE OF MEDICINE   (United States)

Author keywords

RNA seq; Single cell; Tn5 transposase

Indexed keywords

COMPLEMENTARY DNA; CYTOSINE; GUANINE; PROTEIN TN5; RNA; TRANSPOSASE; UNCLASSIFIED DRUG; DNA; TN5 TRANSPOSASE;

ARTICLE; DNA DETERMINATION; DNA RNA HYBRIDIZATION; DNA SYNTHESIS; GENE CONSTRUCT; GENE EXPRESSION; GENE LIBRARY; HETERODUPLEX ANALYSIS; HIGH THROUGHPUT ANALYSIS; HUMAN; HUMAN CELL; NONHUMAN; PRIORITY JOURNAL; REPRODUCIBILITY; REVERSE TRANSCRIPTION; RNA SEQUENCING; CHIMERA; GENETICS; HEK293 CELL LINE; HELA CELL LINE; METABOLISM; PROCEDURES; SEQUENCE ANALYSIS; SINGLE CELL ANALYSIS;

CHIMERA; DNA; DNA, COMPLEMENTARY; GENE LIBRARY; HEK293 CELLS; HELA CELLS; HUMANS; RNA; SEQUENCE ANALYSIS, RNA; SINGLE-CELL ANALYSIS; TRANSPOSASES;

EID: 85079322878     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1919800117     Document Type: Article

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