A simple and novel method for RNA-seq library preparation of single cell cDNA analysis by hyperactive Tn5 transposase

Developmental Dynamics

Volumn 241, Issue 10, 2012, Pages 1584-1590

  Brouilette, Scott ^a,b   Kuersten, Scott ^c   Mein, Charles ^a   Bozek, Monika ^a   Terry, Anna ^a   Dias, Kerith Rae ^a   Bhaw Rosun, Leena ^a   Shintani, Yasunori ^a   Coppen, Steven ^a   Ikebe, Chiho ^a   Sawhney, Vinit ^a   Campbell, Niall ^a   Kaneko, Masahiro ^a   Tano, Nobuko ^a   Ishida, Hidekazu ^a   Suzuki, Ken ^a   Yashiro, Kenta ^a  

^a QUEEN MARY UNIVERSITY OF LONDON   (United Kingdom)

^b Partek Inc   (United States)

^c ILLUMINA INC   (United States)

Author keywords

Library preparation for deep sequencing; RNA seq; Single cell cDNA; Tn5 transposase

Indexed keywords

COMPLEMENTARY DNA; TN5 TRANSPOSASE; TRANSPOSASE; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; DNA DETERMINATION; DNA LIBRARY; EMBRYO; EMBRYONIC STEM CELL; ENZYME MECHANISM; GENE AMPLIFICATION; GENE CONSTRUCT; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; RNA SEQUENCE; SEQUENCE ANALYSIS; SINGLE CELL ANALYSIS; SINGLE CELL CDNA ANALYSIS;

DNA PRIMERS; DNA, COMPLEMENTARY; GENE LIBRARY; HIGH-THROUGHPUT NUCLEOTIDE SEQUENCING; SEQUENCE ANALYSIS, RNA; SONICATION; TRANSPOSASES;

EID: 84866770459     PISSN: 10588388     EISSN: 10970177     Source Type: Journal    
DOI: 10.1002/dvdy.23850     Document Type: Article

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