Tn5 transposase active site mutations suggest position of donor backbone DNA in synaptic complex

Journal of Biological Chemistry

Volumn 278, Issue 3, 2003, Pages 1904-1909

  Peterson, Gregory ^a   Reznikoff, William ^a  

^a UNIVERSITY OF WISCONSIN MADISON   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

AMINO ACIDS; CATALYSIS; DNA; POSITIVE IONS; PROTEINS;

BINDING ASSAYS;

MUTAGENESIS;

DNA; TN5 TRANSPOSASE; TRANSPOSASE; UNCLASSIFIED DRUG; BACTERIAL DNA; PRIMER DNA;

AMINO ACID SEQUENCE; ARTICLE; BINDING ASSAY; CATALYSIS; COMPLEX FORMATION; DNA STRAND BREAKAGE; DNA STRUCTURE; ENZYME ACTIVE SITE; ENZYME BINDING; ENZYME MECHANISM; ENZYME SUBSTRATE COMPLEX; GEOMETRY; NICK END LABELING; NONHUMAN; PRIORITY JOURNAL; PROTEIN DNA INTERACTION; PROTEIN TRANSPORT; SYNAPSE; TRANSPOSON; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; GENETICS; METABOLISM; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; SITE DIRECTED MUTAGENESIS;

TRANSPOSON TN5; TRANSPOSONS;

BASE SEQUENCE; DNA PRIMERS; DNA, BACTERIAL; ELECTROPHORESIS, POLYACRYLAMIDE GEL; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; SUBSTRATE SPECIFICITY; SYNAPSES; TRANSPOSASES;

EID: 0037449821     PISSN: 00219258     EISSN: None     Source Type: Journal    
DOI: 10.1074/jbc.M208968200     Document Type: Article

References (18)

1. Reznikoff, W. S., Bhasin, A., Davies, D. R., Goryshin, I. Y., Mahnke, L. A., Naumann, T., Rayment, I., Steiniger-White, M., and Twining, S. S. (1999) Biochem. Biophys. Res. Commun. 266, 729-734
2. Bhasin, A., Goryshin, I. Y., and Reznikoff, W. S. (1999) J. Biol. Chem. 274, 37021-37029
3. Bhasin, A., Goryshin, I. Y., Steiniger-White, M., York, D., and Reznikoff, W. S. (2000) J. Mol. Biol. 302, 49-63
4. Davies, D. R., Goryshin, I. Y., Reznikoff, W. S., and Rayment, I. (2000) Science 289, 77-85
5. Naumann, T. A., and Reznikoff, W. S. (2000) Proc. Natl. Acad. Sci. U. S. A. 97, 8944-8949
6. Goryshin, I. Y., and Reznikoff, W. S. (1998) J. Biol. Chem. 273, 7367-7374
7. Zhou, M., Bhasin, A., and Reznikoff, W. S. (1998) J. Mol. Biol. 276, 913-925
8. Ason, B., and Reznikoff, W. S. (2002) J. Biol. Chem. 277, 11284-11291
9. Naumann, T. A., and Reznikoff, W. S. (2002) J. Biol. Chem. 277, 17623-17629
10. Davies, D. R., Braam, L. M., Reznikoff, W. S., and Rayment, I. (1999) J. Biol. Chem. 274, 11904-11913
11. Bujacz, G., Jaskolski, M., Alexandratos, J., Wlodawer, A., Merkel, G., Katz, R. A., and Skalka, A. M. (1996) Structure 4, 89-96
12. Bujacz, G., Alexandratos, J., Wlodawer, A., Merkel, G., Andrake, M., Katz, R. A., and Skalka, A. M. (1997) J. Biol. Chem. 272, 18161-18168
13. Goldgur, Y., Dyda, F., Hickman, A. B., Jenkins, T. M., Craigie, R., and Davies, D. R. (1998) Proc. Natl. Acad. Sci. U. S. A. 95, 9150-9154
14. Allingham, J. S., Pribil, P. A., and Haniford, D. B. (1999) J. Mol. Biol. 289, 1195-1206
15. Lovell, S., Goryshin, I. Y., Reznikoff, W. R., and Rayment, I. (2002) Nat. Struct. Biol. 9, 278-281
16. Sambrook, J., Fritsch, E. F., and Maniatis, T. (1989) Molecular Cloning: A Laboratory Manual, Appendix A.1, Cold Spring Harbor Press, Cold Spring Harbor, NY.
17. Horton, R. M., Hunt, H. D., Ho, S. N., Pullen, J. K., and Pease, L. R. (1989) Gene 77, 61-68
18. Bradford, M. M. (1976) Anal. Biochem. 72, 248-254