Relevance and standardization of in vitro antioxidant assays: ABTS, DPPH, and Folin–Ciocalteu

Journal of Chemistry

Volumn 2018, Issue , 2018, Pages

  Abramovič, Helena ^a   Grobin, Blaž ^a   Ulrih, Nataša Poklar ^a   Cigić, Blaž ^a  

^a UNIVERSITY OF LJUBLJANA   (Slovenia)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 85062360644     PISSN: 20909063     EISSN: 20909071     Source Type: Journal    
DOI: 10.1155/2018/4608405     Document Type: Article

References (34)

1. R. G. Berger, S. Lunkenbein, A. Ströhle, and A. Hahn, “Antioxidants in food: mere myth or magic medicine?,” Critical Reviews in Food Science and Nutrition, vol. 52, no. 2, pp. 162–171, 2012.
2. M. C. Foti, “Use and abuse of the DPPH radical,” Journal of Agricultural and Food Chemistry, vol. 63, no. 40, pp. 8765–8776, 2015.
3. R. Apak, M. Mustafa Özyürek, K. Kubilay Güçlü, and E. Esra Çapanoğlu, “Antioxidant activity/capacity measurement. 1. Classification, physicochemical principles, mechanisms, and electron transfer (ET)-based assays,” Journal of Agricultural and Food Chemistry, vol. 64, no. 5, pp. 997–1027, 2016.
4. D. Huang, B. Ou, and R. L. Prior, “The chemistry behind antioxidant capacity assays,” Journal of Agricultural and Food Chemistry, vol. 53, no. 6, pp. 1841–1856, 2005.
5. . radicals by vitamin E is accelerated by its partial ionization: the role of sequential proton loss electron transfer,” Organic Letters, vol. 7, no. 22, pp. 4951–4954, 2005.
6. L. Bertalanič, T. Košmerl, N. Poklar Ulrih, and B. Cigić, “Influence of solvent composition on antioxidant potential of model polyphenols and red wines determined with 2,2-diphenyl-1-picrylhydrazyl,” Journal of Agricultural and Food Chemistry, vol. 60, no. 50, pp. 12282–12288, 2012.
7. • method (Problems in estimation of antioxidant activity),” Food Chemistry, vol. 131, no. 3, pp. 1037–1043, 2012.
8. T. Prevc, N. Šegatin, N. Poklar Ulrih, and B. Cigić, “DPPH assay of vegetable oils and model antioxidants in protic and aprotic solvents,” Talanta, vol. 109, pp. 13–19, 2013.
9. J. Xie and K. M. Schaich, “Re-evaluation of the 2,2-diphenyl-1-picrylhydrazyl free radical (DPPH) assay for antioxidant activity,” Journal of Agricultural and Food Chemistry, vol. 62, no. 19, pp. 4251–4260, 2014.
10. 2 to antioxidant potential of white wine,” Food Chemistry, vol. 174, pp. 147–153, 2015.
11. P. Goupy, C. Dufour, M. Loonis, and O. Dangles, “Quantitative kinetic analysis of hydrogen transfer reactions from dietary polyphenols to the DPPH radical,” Journal of Agricultural and Food Chemistry, vol. 51, no. 3, pp. 615–622, 2003.
12. M. J. Arts, G. R. Haenen, H. P. Voss, and A. Bast, “Antioxidant capacity of reaction products limits the applicability of the trolox equivalent antioxidant capacity (TEAC) assay,” Food and Chemistry Toxicology, vol. 42, no. 1, pp. 45–49, 2004.
13. L. M. Magalhães, M. A. Segundo, S. Reis, and J. L. F. C. Lima, “Methodological aspects about in vitro evaluation of antioxidant properties,” Analytica Chimica Acta, vol. 613, no. 1, pp. 1–19, 2008.
14. K. Thaipong, U. Boonprakob, K. Crosby, L. Cisneros-Zevallos, and D. H. Byrne, “Comparison of ABTS, DPPH, FRAP, and ORAC assays for estimating antioxidant activity from guava fruit extracts,” Journal of Food Composition and Analysis, vol. 19, no. 6-7, pp. 669–675, 2006.
15. A. K. C. Saw, W. S. Yam, K. C. Wong, and C. S. Lai, “A comparative study of the volatile constituents of southeast asian Coffea arabica, Coffea liberica and Coffea robusta green beans and their antioxidant activities,” Journal of Essential Oil Bearing Plants, vol. 18, no. 1, pp. 64–73, 2015.
16. J. A. Vinson, M. Mandarano, M. Hirst, J. R. Trevithick, and P. Bose, “Phenol antioxidant quantity and quality in foods: beers and the effect of two types of beer on an animal model of atherosclerosis,” Journal of Agricultural and Food Chemistry, vol. 51, no. 18, pp. 5528–5533, 2003.
17. C. Delgado-Andrade, J. A. Rufian-Henares, and F. J. Morales, “Assessing the antioxidant activity of melanoidins from coffee brews by different antioxidant methods,” Journal of Agricultural and Food Chemistry, vol. 53, no. 20, pp. 7832–7836, 2005.
18. G. Spigno, L. Tramelli, and D. M. De Faveri, “Effects of extraction time, temperature and solvent on concentration and antioxidant activity of grape marc phenolics,” Journal of Food Engineering, vol. 81, no. 1, pp. 200–208, 2007.
19. F. Shahdadi, H. O. Mirzaei, and A. Daraei Garmakhany, “Study of phenolic compound and antioxidant activity of date fruit as a function of ripening stages and drying process,” Journal of Food Science and Technology, vol. 52, no. 3, pp. 1814–1819, 2015.
20. L. Fu, B. T. Xu, X. R. Xu et al., “Antioxidant capacities and total phenolic contents of 62 fruits,” Food Chemistry, vol. 129, no. 2, pp. 345–350, 2011.
21. A. Floegel, D. O. Kim, S. J. Chung, S. I. Koo, and O. K. Chun, “Comparison of ABTS/DPPH assays to measure antioxidant capacity in popular antioxidant-rich US foods,” Journal of Food Composition and Analysis, vol. 24, no. 7, pp. 1043–1048, 2011.
22. H. Abramovič, B. Grobin, N. Poklar Ulrih, and B. Cigić, “The methodology applied in DPPH, ABTS and Folin-Ciocalteau assays has a large influence on the determined antioxidant potential,” Acta Chimica Slovenica, vol. 64, no. 2, pp. 491–499, 2017.
23. T. Gutfinger, “Polyphenols in olive oils,” Journal of the American Oil Chemists Society, vol. 58, no. 11, pp. 966–968, 1981.
24. W. Brand-Williams, M. E. Cuvelier, and C. Berset, “Use of free radical method to evaluate antioxidant activity,” LWT-Food Science and Technology, vol. 28, no. 1, pp. 25–30, 1995.
25. R. Re, N. Pellegrini, A. Proteggente, A. Pannala, M. Yang, and C. Rice-Evans, “Antioxidant activity applying an improved ABTS radical cation decolorization assay,” Free Radical Biology and Medicine, vol. 26, no. 9–10, pp. 1231–1237, 1999.
26. P. Molyneux, “The use of the stable free radical diphenylpicrylhydrazyl (DPPH) for estimating antioxidant activity,” Songklanakarin Journal of Science and Technology, vol. 26, no. 2, pp. 211–219, 2004.
27. . in semi-aqueous solution,” Organic and Biomolecular Chemistry, vol. 4, no. 12, pp. 2417–2423, 2006.
28. 2 O and aqueous organic solvent mixtures: new accessions and assessments (Technical Report),” Pure and Applied Chemistry, vol. 69, no. 5, pp. 1007–1014, 1997.
29. J. D. Everette, Q. M. Bryant, A. M. Green, Y. A. Abbey, G. W. Wangila, and R. B. Walker, “Thorough study of reactivity of various compound classes toward the Folin−Ciocalteu reagent,” Journal of Agricultural and Food Chemistry, vol. 58, no. 14, pp. 8139–8144, 2010.
30. J. P. Yuan and F. Chen, “Degradation of ascorbic acid in aqueous solution,” Journal of Agricultural and Food Chemistry, vol. 46, no. 12, pp. 5078–5082, 1998.
31. I. Zabetakis, J. W. Gramshaw, and D. S. Robinson, “2,5-Dimethyl-4-hydroxy-2H-furan-3-one and its derivatives: analysis, synthesis and biosynthesis – a review,” Food Chemistry, vol. 65, no. 2, pp. 139–151, 1999.
32. E. N. Delicado, A. S. Ferrer, and F. G. Carmona, “A kinetic study of the one-electron oxidation of Trolox C by the hydroperoxidase activity of lipoxygenase,” Biochimica et Biophysica Acta, vol. 1335, no. 1-2, pp. 127–134, 1997.
33. A. F. Hanham, B. P. Dunn, and H. F. Stich, “Clastogenic activity of caffeic acid and its relationship to hydrogen peroxide generated during autooxidation,” Mutation Research, vol. 116, no. 3-4, pp. 333–339, 1983.
34. D. Granato, F. Shahidi, R. Wrolstad et al., “Antioxidant activity, total phenolics and flavonoids contents: should we ban in vitro screening methods?,” Food Chemistry, vol. 264, pp. 471–475, 2018.