Development of the CRISPR/Cas9 system for targeted gene disruption in Aspergillus fumigatus

Eukaryotic Cell

Volumn 14, Issue 11, 2015, Pages 1073-1080

  Fuller, Kevin K ^a   Chen, Shan ^a   Loros, Jennifer J ^a   Dunlap, Jay C ^a  

^a DARTMOUTH MEDICAL SCHOOL   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

FUNGAL PROTEIN; POLYKETIDE SYNTHASE;

ASPERGILLUS FUMIGATUS; CRISPR CAS SYSTEM; GENE TARGETING; GENETICS; MOLECULAR GENETICS; NUCLEOTIDE SEQUENCE; PROCEDURES;

ASPERGILLUS FUMIGATUS; BASE SEQUENCE; CRISPR-CAS SYSTEMS; FUNGAL PROTEINS; GENE TARGETING; MOLECULAR SEQUENCE DATA; POLYKETIDE SYNTHASES;

EID: 85047289483     PISSN: 15359778     EISSN: None     Source Type: Journal    
DOI: 10.1128/EC.00107-15     Document Type: Article

References (41)

1. Rhodes JC. 2006. Aspergillus fumigatus: growth and virulence. Med Mycol 44(Suppl 1):S77-S81.
2. Askew DS. 2008. Aspergillus fumigatus: virulence genes in a street-smart mold. Curr Opin Microbiol 11:331-337. http://dx.doi.org/10.1016/j.mib.2008.05.009.
3. Upton A, Kirby KA, Carpenter P, Boeckh M, Marr KA. 2007. Invasive aspergillosis following hematopoietic cell transplantation: outcomes and prognostic factors associated with mortality. Clin Infect Dis 44:531-540. http://dx.doi.org/10.1086/510592.
4. Segal BH. 2009. Aspergillosis. N Engl J Med 360:1870-1884. http://dx.doi.org/10.1056/NEJMra0808853.
5. Singh N, Paterson DL. 2005. Aspergillus infections in transplant recipients. Clin Microbiol Rev 18:44-69. http://dx.doi.org/10.1128/CMR.18.1.44-69.2005.
6. Firon A, Villalba F, Beffa R, d’Enfert C. 2003. Identification of essential genes in the human fungal pathogen Aspergillus fumigatus by transposon mutagenesis. Eukaryot Cell 2:247-255. http://dx.doi.org/10.1128/EC.2.2.247-255.2003.
7. Jackson J, Higgins L, Lin X. 2009. Conidiation color mutants of Aspergillus fumigatus are highly pathogenic to the heterologous insect host Galleria mellonella. PLoS One 4:e4224. http://dx.doi.org/10.1371/journal.pone.0004224.
8. Manavathu EK, Alangaden GJ, Chandrasekar PH. 1998. In-vitro isolation and antifungal susceptibility of amphotericin B-resistant mutants of Aspergillus fumigatus. J Antimicrob Chemother 41:615-619. http://dx.doi.org/10.1093/jac/41.6.615.
9. da Silva Ferreira ME, Kress MRVZ, Savoldi M, Goldman MHS, Härtl A, Heinekamp T, Brakhage A, Goldman GH. 2006. The akuBKU80 mutant deficient for nonhomologous end joining is a powerful tool for analyzing pathogenicity in Aspergillus fumigatus. Eukaryot Cell 5:207-211. http://dx.doi.org/10.1128/EC.5.1.207-211.2006.
10. Krappmann S, Sasse C, Braus GH. 2006. Gene targeting in Aspergillus fumigatus by homologous recombination is facilitated in a nonhomologous end-joining-deficient genetic background. Eukaryot Cell 5:212-215. http://dx.doi.org/10.1128/EC.5.1.212-215.2006.
11. Gravelat F, Askew D, Sheppard D. 2012. Targeted gene deletion in Aspergillus fumigatus using the hygromycin-resistance split-marker approach. Methods Mol Biol 845:119-130. http://dx.doi.org/10.1007/978-1-61779-539-8_8.
12. D’Enfert C, Diaquin M, Delit A, Wuscher N, Debeaupuis JP, Huerre M, Latge JP. 1996. Attenuated virulence of uridine-uracil auxotrophs of Aspergillus fumigatus. Infect Immun 64:4401-4405.
13. Mouyna I, Henry C, Doering T, Latgé J. 2004. Gene silencing with RNA interference in the human pathogenic fungus Aspergillus fumigatus. FEMS Microbiol Lett 15:317-324.
14. Henry C, Mouyna I, Latgé J. 2007. Testing the efficacy of RNA interference constructs in Aspergillus fumigatus. Curr Genet 51:277-284. http://dx.doi.org/10.1007/s00294-007-0119-0.
15. Horvath P, Barrangou R. 2010. CRISPR/Cas, the immunes system of Bacteria and Archaea. Science 327:167-170. http://dx.doi.org/10.1126/science.1179555.
16. Wiedenheft B, Sternberg SH, Doudna JA. 2012. RNA-guided genetic silencing systems in bacteria and archaea. Nature 482:331-338. http://dx.doi.org/10.1038/nature10886.
17. Jinek M, Chylinski K, Fonfara I, Hauer M, Doudna JA, Charpentier E. 2012. A programmable dual-RNA- guided DNA endonuclease in adaptive bacterial immunity. Science 337:816-821. http://dx.doi.org/10.1126/science.1225829.
18. Cong L, Ran FA, Cox D, Lin S, Barretto R, Habib N, Hsu PD, Wu X, Jiang W, Marraffini LA, Zhang F. 2013. Multiplex genome engineering using CRISPR/Cas systems. Science 339:819-823. http://dx.doi.org/10.1126/science.1231143.
19. Wang H, Yang H, Shivalila C, Dawlaty MM, Cheng A, Zhang F, Jaenisch R. 2013. One-step generation of mice carrying mutations in multiple genes by CRISPR/Cas-mediated genome engineering. Cell 153: 910-918. http://dx.doi.org/10.1016/j.cell.2013.04.025.
20. Shen B, Zhang J, Wu H, Wang J, Ma K, Li Z, Zhang X, Zhang P, Huang X. 2013. Generation of gene-modified mice via Cas9/RNAmediated gene targeting. Cell Res 23:720-723. http://dx.doi.org/10.1038/cr.2013.46.
21. Li D, Qiu Z, Shao Y, Chen Y, Guan Y, Liu M, Li Y, Gao N, Wang L, Lu X, Zhao Y, Liu M. 2013. Heritable gene targeting in the mouse and rat using a CRISPR-Cas system. Nat Biotechnol 31:681-683. http://dx.doi.org/10.1038/nbt.2661.
22. Jiang W, Zhou H, Bi H, Fromm M, Yang B, Weeks DP. 2013. Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice. Nucleic Acids Res 41:e188-e188. http://dx.doi.org/10.1093/nar/gkt780.
23. Shan Q, Wang Y, Li J, Zhang Y, Chen K, Liang Z, Zhang K, Liu J, Xi JJ, Qiu JL, Gao C. 2013. Targeted genome modification of crop plants using a CRISPR-Cas system. Nat Biotechnol 31:686-688. http://dx.doi.org/10.1038/nbt.2650.
24. DiCarlo JE, Norville JE, Mali P, Rios X, Aach J, Church GM. 2013. Genome engineering in Saccharomyces cerevisiae using CRISPR-Cas systems. Nucleic Acids Res 41:4336-4343. http://dx.doi.org/10.1093/nar/gkt135.
25. Vyas VK, Barrasa MI, Fink GR. 2015. A Candida albicans CRISPR system permits genetic engineering of essential genes and gene families. Sci Adv 1:e1500248. http://dx.doi.org/10.1126/sciadv.1500248.
26. Rui L, Chen L, Jiang Y, Zhou Z, Zou G. 2015. Efficient genome editing in filamentous fungus Trichoderma reesei using the CRISPR/Cas9 system. Cell Discovery 1:15007. http://dx.doi.org/10.1038/celldisc.2015.7.
27. Nødvig CS, Nielsen JB, Kogle ME, Mortensen UH. 2015. A CRISPRCas9 system for genetic engineering of filamentous fungi. PLoS One 10: e0133085. http://dx.doi.org/10.1371/journal.pone.0133085.
28. Jiang W, Bikard D, Cox D, Zhang F, Marraffini LA. 2013. RNA-guided editing of bacterial genomes using CRISPR-Cas systems. Nat Biotechnol 31:233-239. http://dx.doi.org/10.1038/nbt.2508.
29. Pattanayak V, Lin S, Guilinger JP, Ma E, Doudna JA, Liu DR. 2013. High-throughput profiling of off-target DNA cleavage reveals RNAprogrammed Cas9 nuclease specificity. Nat Biotechnol 31:839-843. http://dx.doi.org/10.1038/nbt.2673.
30. Langfelder K, Jahn B, Gehringer H, Schmidt A, Wanner G, Brakhage AA. 1998. Identification of a polyketide synthase gene (pksP) of Aspergillus fumigatus involved in conidial pigment biosynthesis and virulence. Med Microbiol Immunol 187:79-89. http://dx.doi.org/10.1007/s004300050077.
31. Tsai HF, Chang YC, Washburn RG, Wheeler MH, Kwon-Chung KJ. 1998. The developmentally regulated alb1 gene of Aspergillus fumigatus: its role in modulation of conidial morphology and virulence. J Bacteriol 180: 3031-3038.
32. Oldenburg KR, Vo KT, Michaelis S, Paddon C. 1997. Recombinationmediated PCR-directed plasmid construction in vivo in yeast. Nucleic Acids Res 25:451-452. http://dx.doi.org/10.1093/nar/25.2.451.
33. Silar P. 1995. Two new easy to use vectors for transformations. Fungal Genet News Lett 42:73.
34. Yelton MM, Hamer JE, Timberlake WE. 1984. Transformation of Aspergillus nidulans by using a trpC plasmid 81:1470-1474.
35. Thön M, Al-Abdallah Q, Hortschansky P, Brakhage AA. 2007. The thioredoxin system of the filamentous fungus Aspergillus nidulans: impact on development and oxidative stress response. J Biol Chem 282:27259-27269. http://dx.doi.org/10.1074/jbc.M704298200.
36. Ram AF, Klis FM. 2006. Identification of fungal cell wall mutants using susceptibility assays based on Calcofluor white and Congo red. Nat Protoc 1:2253-2256. http://dx.doi.org/10.1038/nprot.2006.397.
37. Ghannoum MA, Rice LB. 1999. Antifungal agents: mode of action, mechanisms of resistance, and correlation of these mechanisms with bacterial resistance. Clin Microbiol Rev 12:501-517.
38. Sugui JA, Chang YC, Kwon-Chung KJ. 2005. Agrobacterium tumefaciens-mediated transformation of Aspergillus fumigatus: an efficient tool for insertional mutagenesis and targeted gene disruption. Appl Environ Microbiol 71:1798-1802. http://dx.doi.org/10.1128/AEM.71.4.1798-1802.2005.
39. Qiao JJ, Liu W, Ma Y, Wan Z, Li RY. 2008. Agrobacterium tumefaciensmediated transformation of uracil auxotroph Aspergillus fumigatus is an efficient method for target gene knockout. Beijing Da Xue Xue Bao 40: 330-333.
40. Bachu R, Bergareche I, Chasin LA. 2015. CRISPR-Cas targeted plasmid integration into mammalian cells via non-homologous end joining. Biotechnol Bioeng. 112:2154-2162. http://dx.doi.org/10.1002/bit.25629.
41. Smulian AG, Gibbons RS, Demland JA, Spaulding DT, Deepe GS, Jr. 2007. Expression of hygromycin phosphotransferase alters virulence of Histoplasma capsulatum. Eukaryot Cell 6:2066-2071. http://dx.doi.org/10.1128/EC.00139-07.