Gene targeting in Aspergillus fumigatus by homologous recombination is facilitated in a nonhomologous end-joining-deficient genetic background

Eukaryotic Cell

Volumn 5, Issue 1, 2006, Pages 212-215

  Krappmann, Sven ^a,b   Sasse, Christoph ^a   Braus, Gerhard H ^a  

^a UNIVERSITY OF GÖTTINGEN   (Germany)

^b Institute of Microbiology and Genetics   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CELL NUCLEUS ANTIGEN; DNA BINDING PROTEIN; KU ANTIGEN;

ARTICLE; ASPERGILLUS FUMIGATUS; CYTOLOGY; FUNGAL GENE; GENE DELETION; GENE TARGETING; GENETIC RECOMBINATION; GENETICS; METHODOLOGY; PATHOGENICITY;

ANTIGENS, NUCLEAR; ASPERGILLUS FUMIGATUS; DNA-BINDING PROTEINS; GENE DELETION; GENE TARGETING; GENES, FUNGAL; RECOMBINATION, GENETIC;

ASPERGILLUS FUMIGATUS;

EID: 30944467252     PISSN: 15359778     EISSN: None     Source Type: Journal    
DOI: 10.1128/EC.5.1.212-215.2006     Document Type: Article

References (15)

1. Bird, D., and R. Bradshaw. 1997. Gene targeting is locus dependent in the filamentous fungus Aspergillus nidulans. Mol. Gen. Genet. 255:219-225.
2. Borkovich, K. A., L. A. Alex, O. Yarden, M. Freitag, G. E. Turner, N. D. Read, S. Seiler, D. Bell-Pedersen, J. Paietta, N. Plesofsky, M. Plamann, M. Goodrich-Tanrikulu, U. Schulte, G. Mannhaupt, F. E. Nargang, A. Radford, C. Selitrennikoff, J. E. Galagan, J. C. Dunlap, J. J. Loros, D. Catcheside, H. Inoue, R. Aramayo, M. Polymenis, E. U. Selker, M. S. Sachs, G. A. Marzluf, I. Paulsen, R. Davis, D. J. Ebbole, A. Zelter, E. R. Kalkman, R. O'Rourke, F. Bowring, J. Yeadon, C. Ishii, K. Suraki, W. Sakai, and R. Pratt. 2004. Lessons from the genome sequence of Neurospora crassa: tracing the path from genomic blueprint to multicellular organism. Microbiol. Mol. Biol. Rev. 68:1-108.
3. Brakhage, A. A., and K. Langfelder. 2002. Menacing mold: the molecular biology of Aspergillus fumigatus. Annu. Rev. Microbiol. 56:433-455.
4. Critchlow, S. E., and S. P. Jackson. 1998. DNA end-joining: from yeast to man. Trends Biochem. Sci. 23:394-398.
5. Kämper, J. 2004. A PCR-based system for highly efficient generation of gene replacement mutants in Ustilago maydis. Mol. Genet. Genomics 271:103-110.
6. Kavanagh, K., and E. P. Reeves. 2004. Exploiting the potential of insects for in vivo pathogenicity testing of microbial pathogens. FEMS Microbiol. Rev. 28:101-112.
7. Krappmann, S., Ö. Bayram, and G. H. Braus. 2005. Deletion and allelic exchange of the Aspergillus fumigatus veA locus via a novel recyclable marker module. Eukaryot. Cell 4:1298-1307.
8. Kubodera, T., N. Yamashita, and A. Nishimura. 2000. Pyrithiamine resistance gene (ptrA) of Aspergillus oryzae: cloning, characterization and application as a dominant selectable marker for transformation. Biosci. Biotechnol. Biochem. 64:1416-1421.
9. Maerker, C., M. Rohde, A. A. Brakhage, and M. Brock. 2005. Methylcitrate synthase from Aspergillus fumigatus. FEBS J. 272:3615-3630.
10. Ninomiya, Y., K. Suzuki, C. Ishii, and H. Inoue. 2004. Highly efficient gene replacements in Neurospora strains deficient for nonhomologous end-joining. Proc. Natl. Acad. Sci. USA 101:12248-12253.
11. Punt, P. J., M. A. Dingemanse, A. Kuyvenhoven, R. D. Soede, P. H. Pouwels, and C. A. van den Hondel. 1990. Functional elements in the promoter region of the Aspergillus nidulans gpdA gene encoding glyceraldehyde-3-phosphate dehydrogenase. Gene. 93:101-109.
12. Punt, P. J., and C. A. van den Hondel. 1992. Transformation of filamentous fungi based on hygromycin B and phleomycin resistance markers. Methods Enzymol. 216:447-457.
13. Reichard, U., S. Buttner, H. Eiffert, F. Staib, and R. Rüchel. 1990. Purification and characterisation of an extracellular serine proteinase from Aspergillus fumigatus and its detection in tissue. J. Med. Microbiol. 33:243-251.
14. Southern, E. M. 1975. Detection of specific sequences among DNA fragments separated by gel electrophoresis. J. Mol. Biol. 98:503-517.
15. Tsai, H. F., M. H. Wheeler, Y. C. Chang, and K. J. Kwon-Chung. 1999. A developmentally regulated gene cluster involved in conidial pigment biosynthesis in Aspergillus fumigatus. J. Bacteriol. 181:6469-6477.