Generation of innate immune reporter cells using retroviral transduction

Methods in Molecular Biology

Volumn 1714, Issue , 2018, Pages 97-117

  Gloria, Yamel Cardona ^a   Latz, Eicke ^b,c,d   De Nardo, Dominic ^e,f  

^a UNIVERSITY OF TÜBINGEN   (Germany)

^b UNIVERSITY HOSPITAL BONN   (Germany)

^c UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

^d GERMAN CENTER FOR NEURODEGENERATIVE DISEASES DZNE   (Germany)

^e WALTER AND ELIZA HALL INSTITUTE OF MEDICAL RESEARCH   (Australia)

^f UNIVERSITY OF MELBOURNE   (Australia)

Author keywords

Bone marrow isolation; Bone marrow derived macrophages; Fluorescent protein; Infection; Innate immune reporter cells; Innate immunity; Retrovirus; Transduction

Indexed keywords

ANTIBIOTIC AGENT; RETROVIRUS VECTOR; TOLL LIKE RECEPTOR 4; GREEN FLUORESCENT PROTEIN;

ADHERENT CELL; ANIMAL TISSUE; ANTIBIOTIC RESISTANCE; BONE MARROW CELL; CELL COUNT; CELL CULTURE; CELL DEATH; CELL DENSITY; CELL DIFFERENTIATION; CELL MATURATION; CONTROLLED STUDY; GENOTYPE; HEALTH HAZARD; HEK293T CELL LINE; HINDLIMB; HUMAN; HUMAN CELL; INNATE IMMUNITY; LAMINAR FLOW; MACROPHAGE; MOUSE; NONHUMAN; RETROVIRIDAE; RETROVIRUS INFECTION; TARGET CELL; TISSUE CULTURE; TLR SIGNALING; TRANSIENT TRANSFECTION; VIRAL GENE DELIVERY SYSTEM; ANIMAL; CYTOLOGY; GENE VECTOR; GENETIC TRANSDUCTION; GENETICS; HEK293 CELL LINE; METABOLISM; PHYSIOLOGY; PROCEDURES;

ANIMALS; BONE MARROW CELLS; CELL DIFFERENTIATION; CELLS, CULTURED; GENETIC VECTORS; GREEN FLUORESCENT PROTEINS; HEK293 CELLS; HUMANS; IMMUNITY, INNATE; MACROPHAGES; MICE; RETROVIRIDAE; TRANSDUCTION, GENETIC;

EID: 85035337144     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-4939-7519-8_7     Document Type: Chapter

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