메뉴 건너뛰기




Volumn 53, Issue , 2018, Pages 20-25

Engineering microorganisms for improving polyhydroxyalkanoate biosynthesis

Author keywords

[No Author keywords available]

Indexed keywords

BIOSYNTHESIS; CELL ENGINEERING;

EID: 85034605398     PISSN: 09581669     EISSN: 18790429     Source Type: Journal    
DOI: 10.1016/j.copbio.2017.10.008     Document Type: Review
Times cited : (83)

References (62)
  • 1
    • 84859038271 scopus 로고    scopus 로고
    • Plastics derived from biological sources: present and future: a technical and environmental review
    • Chen, G.Q., Patel, M.K., Plastics derived from biological sources: present and future: a technical and environmental review. Chem Rev 112 (2012), 2082–2099.
    • (2012) Chem Rev , vol.112 , pp. 2082-2099
    • Chen, G.Q.1    Patel, M.K.2
  • 2
    • 82755198036 scopus 로고    scopus 로고
    • Polyhydroxyalkanoates as a source of chemicals, polymers, and biofuels
    • Gao, X., Chen, J.C., Wu, Q., Chen, G.Q., Polyhydroxyalkanoates as a source of chemicals, polymers, and biofuels. Curr Opin Biotechnol 22 (2011), 768–774.
    • (2011) Curr Opin Biotechnol , vol.22 , pp. 768-774
    • Gao, X.1    Chen, J.C.2    Wu, Q.3    Chen, G.Q.4
  • 3
    • 84874388995 scopus 로고    scopus 로고
    • The chemomechanical properties of microbial polyhydroxyalkanoates
    • Laycock, B., Halley, P., Pratt, S., Werker, A., Lant, P., The chemomechanical properties of microbial polyhydroxyalkanoates. Prog Polym Sci 38 (2013), 536–583.
    • (2013) Prog Polym Sci , vol.38 , pp. 536-583
    • Laycock, B.1    Halley, P.2    Pratt, S.3    Werker, A.4    Lant, P.5
  • 4
    • 0032900321 scopus 로고    scopus 로고
    • Factors affecting the economics of polyhydroxyalkanoate production by bacterial fermentation
    • Choi, J., Lee, S.Y., Factors affecting the economics of polyhydroxyalkanoate production by bacterial fermentation. Appl Microbiol Biotechnol 51 (1999), 13–21.
    • (1999) Appl Microbiol Biotechnol , vol.51 , pp. 13-21
    • Choi, J.1    Lee, S.Y.2
  • 5
    • 84896926470 scopus 로고    scopus 로고
    • Valorization of industrial waste and by-product streams via fermentation for the production of chemicals and biopolymers
    • This review focuses on the potential importance of utilizing waste and by-product streams from current industrial activities for the production of chemicals and biopolymers via microbial bioconversion.
    • Koutinas, A.A., Vlysidis, A., Pleissner, D., Kopsahelis, N., Lopez Garcia, I., Kookos, I.K., Papanikolaou, S., Kwan, T.H., Lin, C.S., Valorization of industrial waste and by-product streams via fermentation for the production of chemicals and biopolymers. Chem Soc Rev 43 (2014), 2587–2627 This review focuses on the potential importance of utilizing waste and by-product streams from current industrial activities for the production of chemicals and biopolymers via microbial bioconversion.
    • (2014) Chem Soc Rev , vol.43 , pp. 2587-2627
    • Koutinas, A.A.1    Vlysidis, A.2    Pleissner, D.3    Kopsahelis, N.4    Lopez Garcia, I.5    Kookos, I.K.6    Papanikolaou, S.7    Kwan, T.H.8    Lin, C.S.9
  • 6
    • 4544314878 scopus 로고    scopus 로고
    • Metabolic engineering and pathway construction for biotechnological production of relevant polyhydroxyalkanoates in microorganisms
    • Steinbüchel, A., Lütke-Eversloh, T., Metabolic engineering and pathway construction for biotechnological production of relevant polyhydroxyalkanoates in microorganisms. Biochem Eng J 16 (2003), 81–96.
    • (2003) Biochem Eng J , vol.16 , pp. 81-96
    • Steinbüchel, A.1    Lütke-Eversloh, T.2
  • 7
    • 84903383582 scopus 로고    scopus 로고
    • Microbial polyhydroxyalkanoates, challenges and opportunities
    • Wang, Y., Yin, J., Chen, G.Q., Microbial polyhydroxyalkanoates, challenges and opportunities. Curr Opin Biotechnol 30C (2014), 59–65.
    • (2014) Curr Opin Biotechnol , vol.30C , pp. 59-65
    • Wang, Y.1    Yin, J.2    Chen, G.Q.3
  • 8
    • 69249092516 scopus 로고    scopus 로고
    • A microbial polyhydroxyalkanoates (PHA) based bio- and materials industry
    • Chen, G.Q., A microbial polyhydroxyalkanoates (PHA) based bio- and materials industry. Chem Soc Rev 38 (2009), 2434–2446.
    • (2009) Chem Soc Rev , vol.38 , pp. 2434-2446
    • Chen, G.Q.1
  • 9
    • 84867703155 scopus 로고    scopus 로고
    • Advanced bacterial polyhydroxyalkanoates: towards a versatile and sustainable platform for unnatural tailor-made polyesters
    • Park, S.J., Kim, T.W., Kim, M.K., Lee, S.Y., Lim, S.C., Advanced bacterial polyhydroxyalkanoates: towards a versatile and sustainable platform for unnatural tailor-made polyesters. Biotechnol Adv 30 (2012), 1196–1206.
    • (2012) Biotechnol Adv , vol.30 , pp. 1196-1206
    • Park, S.J.1    Kim, T.W.2    Kim, M.K.3    Lee, S.Y.4    Lim, S.C.5
  • 10
    • 0345688125 scopus 로고    scopus 로고
    • Polyester synthases: natural catalysts for plastics
    • Bernd, H., Polyester synthases: natural catalysts for plastics. Biochem J 376 (2003), 15–33.
    • (2003) Biochem J , vol.376 , pp. 15-33
    • Bernd, H.1
  • 11
    • 77950858901 scopus 로고    scopus 로고
    • Analysis of polyhydroxybutyrate flux limitations by systematic genetic and metabolic perturbations
    • In this study, growth rate and PHB flux were quantified directly in response to systematic manipulation of (1) gene expression in the product-forming pathway and (2) growth rates in a nitrogen-limited chemostat. They demonstrated that acetoacetyl-CoA reductase (phaB) limits flux to PHB.
    • Tyo, K.E., Fischer, C.R., Simeon, F., Stephanopoulos, G., Analysis of polyhydroxybutyrate flux limitations by systematic genetic and metabolic perturbations. Metab Eng 12 (2010), 187–195 In this study, growth rate and PHB flux were quantified directly in response to systematic manipulation of (1) gene expression in the product-forming pathway and (2) growth rates in a nitrogen-limited chemostat. They demonstrated that acetoacetyl-CoA reductase (phaB) limits flux to PHB.
    • (2010) Metab Eng , vol.12 , pp. 187-195
    • Tyo, K.E.1    Fischer, C.R.2    Simeon, F.3    Stephanopoulos, G.4
  • 12
    • 0030725937 scopus 로고    scopus 로고
    • Poly (3-hydroxybutyrate) production from whey using recombinant Escherichia coli
    • Lee, S., Middelberg, A., Lee, Y., Poly (3-hydroxybutyrate) production from whey using recombinant Escherichia coli. Biotechnol Lett 19 (1997), 1033–1035.
    • (1997) Biotechnol Lett , vol.19 , pp. 1033-1035
    • Lee, S.1    Middelberg, A.2    Lee, Y.3
  • 13
    • 85020514019 scopus 로고    scopus 로고
    • Controlling microbial PHB synthesis via CRISPRi
    • Li, D., Lv, L., Chen, J.C., Chen, G.Q., Controlling microbial PHB synthesis via CRISPRi. Appl Microbiol Biotechnol 101 (2017), 5861–5867.
    • (2017) Appl Microbiol Biotechnol , vol.101 , pp. 5861-5867
    • Li, D.1    Lv, L.2    Chen, J.C.3    Chen, G.Q.4
  • 14
    • 84891860323 scopus 로고    scopus 로고
    • Metabolic engineering of Escherichia coli for biosynthesis of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) from glucose
    • Yang, J.E., Choi, Y.J., Lee, S.J., Kang, K.-H., Lee, H., Oh, Y.H., Lee, S.H., Park, S.J., Lee, S.Y., Metabolic engineering of Escherichia coli for biosynthesis of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) from glucose. Appl Microbiol Biotechnol 98 (2014), 95–104.
    • (2014) Appl Microbiol Biotechnol , vol.98 , pp. 95-104
    • Yang, J.E.1    Choi, Y.J.2    Lee, S.J.3    Kang, K.-H.4    Lee, H.5    Oh, Y.H.6    Lee, S.H.7    Park, S.J.8    Lee, S.Y.9
  • 16
    • 84888397225 scopus 로고    scopus 로고
    • Production of block copolymer poly(3-hydroxybutyrate)-block-poly(3-hydroxypropionate) with adjustable structure from an inexpensive carbon source
    • Wang, Q., Yang, P., Xian, M., Liu, H., Cao, Y., Yang, Y., Zhao, G., Production of block copolymer poly(3-hydroxybutyrate)-block-poly(3-hydroxypropionate) with adjustable structure from an inexpensive carbon source. ACS Macro Lett 2 (2013), 996–1000.
    • (2013) ACS Macro Lett , vol.2 , pp. 996-1000
    • Wang, Q.1    Yang, P.2    Xian, M.3    Liu, H.4    Cao, Y.5    Yang, Y.6    Zhao, G.7
  • 17
    • 84917686886 scopus 로고    scopus 로고
    • Impact on poly (3-hydroxybutyrate) storage in recombinant Escherichia coli by Ralstonia eutropha's PHB depolymerases and phasins
    • AEM.02666-02614
    • Eggers, J., Steinbüchel, A., Impact on poly (3-hydroxybutyrate) storage in recombinant Escherichia coli by Ralstonia eutropha's PHB depolymerases and phasins. Appl Environ Microbiol, 2014, 10.1128/AEM.02666-14 AEM.02666-02614.
    • (2014) Appl Environ Microbiol
    • Eggers, J.1    Steinbüchel, A.2
  • 18
    • 0000979635 scopus 로고    scopus 로고
    • Production of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) by high-cell-density cultivation of Aeromonas hydrophila
    • Lee, S.H., Oh, D.H., Ahn, W.S., Lee, Y., Choi Ji, Lee, S.Y., Production of poly (3-hydroxybutyrate-co-3-hydroxyhexanoate) by high-cell-density cultivation of Aeromonas hydrophila. Biotechnol Bioeng 67 (2000), 240–244.
    • (2000) Biotechnol Bioeng , vol.67 , pp. 240-244
    • Lee, S.H.1    Oh, D.H.2    Ahn, W.S.3    Lee, Y.4    Choi Ji5    Lee, S.Y.6
  • 19
    • 61449182522 scopus 로고    scopus 로고
    • Effect of anaerobic promoters on the microaerobic production of polyhydroxybutyrate (PHB) in recombinant Escherichia coli
    • Wei, X.X., Shi, Z.Y., Yuan, M.Q., Chen, G.Q., Effect of anaerobic promoters on the microaerobic production of polyhydroxybutyrate (PHB) in recombinant Escherichia coli. Appl Microbiol Biotechnol, 82, 2009, 703.
    • (2009) Appl Microbiol Biotechnol , vol.82 , pp. 703
    • Wei, X.X.1    Shi, Z.Y.2    Yuan, M.Q.3    Chen, G.Q.4
  • 20
    • 13544255492 scopus 로고    scopus 로고
    • Kinetic studies and biochemical pathway analysis of anaerobic poly-(R)-3-hydroxybutyric acid synthesis in Escherichia coli
    • Carlson, R., Wlaschin, A., Srienc, F., Kinetic studies and biochemical pathway analysis of anaerobic poly-(R)-3-hydroxybutyric acid synthesis in Escherichia coli. Appl Environ Microbiol 71 (2005), 713–720.
    • (2005) Appl Environ Microbiol , vol.71 , pp. 713-720
    • Carlson, R.1    Wlaschin, A.2    Srienc, F.3
  • 21
    • 38049156419 scopus 로고    scopus 로고
    • Poly (3-hydroxybutyrate) synthesis from glycerol by a recombinant Escherichia coli arcA mutant in fed-batch microaerobic cultures
    • Nikel, P.I., Pettinari, M.J., Galvagno, M.A., Méndez, B.S., Poly (3-hydroxybutyrate) synthesis from glycerol by a recombinant Escherichia coli arcA mutant in fed-batch microaerobic cultures. Appl Microbiol Biotechnol 77 (2008), 1337–1343.
    • (2008) Appl Microbiol Biotechnol , vol.77 , pp. 1337-1343
    • Nikel, P.I.1    Pettinari, M.J.2    Galvagno, M.A.3    Méndez, B.S.4
  • 22
    • 78650544471 scopus 로고    scopus 로고
    • Biosynthesis of poly (3-hydroxydecanoate) and 3-hydroxydodecanoate dominating polyhydroxyalkanoates by β-oxidation pathway inhibited Pseudomonas putida
    • In this study, several fatty acid degradation enzymes were deleted to allow PHA structure controls and enhanced fatty acid conversion to PHA.
    • Liu, Q., Luo, G., Zhou, X.R., Chen, G.Q., Biosynthesis of poly (3-hydroxydecanoate) and 3-hydroxydodecanoate dominating polyhydroxyalkanoates by β-oxidation pathway inhibited Pseudomonas putida. Metab Eng 13 (2011), 11–17 In this study, several fatty acid degradation enzymes were deleted to allow PHA structure controls and enhanced fatty acid conversion to PHA.
    • (2011) Metab Eng , vol.13 , pp. 11-17
    • Liu, Q.1    Luo, G.2    Zhou, X.R.3    Chen, G.Q.4
  • 23
    • 58049208259 scopus 로고    scopus 로고
    • Production and characterization of homopolymer polyhydroxyheptanoate (P3HHp) by a fadBA knockout mutant Pseudomonas putida KTOY06 derived from P. putida KT2442
    • Wang, H.H., Li, X.T., Chen, G.Q., Production and characterization of homopolymer polyhydroxyheptanoate (P3HHp) by a fadBA knockout mutant Pseudomonas putida KTOY06 derived from P. putida KT2442. Process Biochem 44 (2009), 106–111.
    • (2009) Process Biochem , vol.44 , pp. 106-111
    • Wang, H.H.1    Li, X.T.2    Chen, G.Q.3
  • 24
    • 80053938042 scopus 로고    scopus 로고
    • Biosynthesis and characterization of poly (3-hydroxydodecanoate) by β-oxidation inhibited mutant of Pseudomonas entomophila L48
    • Chung, A.L., Jin, H.L., Huang, L.J., Ye, H.M., Chen, J.C., Wu, Q., Chen, G.Q., Biosynthesis and characterization of poly (3-hydroxydodecanoate) by β-oxidation inhibited mutant of Pseudomonas entomophila L48. Biomacromolecules 12 (2011), 3559–3566.
    • (2011) Biomacromolecules , vol.12 , pp. 3559-3566
    • Chung, A.L.1    Jin, H.L.2    Huang, L.J.3    Ye, H.M.4    Chen, J.C.5    Wu, Q.6    Chen, G.Q.7
  • 25
    • 84896121349 scopus 로고    scopus 로고
    • Development of Halomonas TD01 as a host for open production of chemicals
    • Fu, X.Z., Tan, D., Aibaidula, G., Wu, Q., Chen, J.C., Chen, G.Q., Development of Halomonas TD01 as a host for open production of chemicals. Metab Eng 23 (2014), 78–91.
    • (2014) Metab Eng , vol.23 , pp. 78-91
    • Fu, X.Z.1    Tan, D.2    Aibaidula, G.3    Wu, Q.4    Chen, J.C.5    Chen, G.Q.6
  • 26
    • 77953233224 scopus 로고    scopus 로고
    • Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) from unrelated carbon sources by metabolically engineered Escherichia coli
    • Li, Z.J., Shi, Z.Y., Jian, J., Guo, Y.Y., Wu, Q., Chen, G.Q., Production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) from unrelated carbon sources by metabolically engineered Escherichia coli. Metab Eng 12 (2010), 352–359.
    • (2010) Metab Eng , vol.12 , pp. 352-359
    • Li, Z.J.1    Shi, Z.Y.2    Jian, J.3    Guo, Y.Y.4    Wu, Q.5    Chen, G.Q.6
  • 27
    • 75249095758 scopus 로고    scopus 로고
    • Conversion of glycerol to poly (3-hydroxypropionate) in recombinant Escherichia coli
    • Andreeßen, B., Lange, A.B., Robenek, H., Steinbüchel, A., Conversion of glycerol to poly (3-hydroxypropionate) in recombinant Escherichia coli. Appl Environ Microbiol 76 (2010), 622–626.
    • (2010) Appl Environ Microbiol , vol.76 , pp. 622-626
    • Andreeßen, B.1    Lange, A.B.2    Robenek, H.3    Steinbüchel, A.4
  • 28
    • 0037940280 scopus 로고    scopus 로고
    • Medium-chain-length polyhydroxyalkanoic acids (PHA mcl) produced by Pseudomonas putida IPT 046 from renewable sources
    • Sánchez, R.J., Schripsema, J., da Silva, L.F., Taciro, M.K., Pradella, J.G., Gomez, J.G.C., Medium-chain-length polyhydroxyalkanoic acids (PHA mcl) produced by Pseudomonas putida IPT 046 from renewable sources. Euro Polym J 39 (2003), 1385–1394.
    • (2003) Euro Polym J , vol.39 , pp. 1385-1394
    • Sánchez, R.J.1    Schripsema, J.2    da Silva, L.F.3    Taciro, M.K.4    Pradella, J.G.5    Gomez, J.G.C.6
  • 29
    • 0036715480 scopus 로고    scopus 로고
    • Biochemical observations on medium-chain-length polyhydroxyalkanoate biosynthesis and accumulation in Pseudomonas mendocina
    • Kroumova, A.B., Wagner, G.J., Davies, H.M., Biochemical observations on medium-chain-length polyhydroxyalkanoate biosynthesis and accumulation in Pseudomonas mendocina. Arch Biochem Biophys 405 (2002), 95–103.
    • (2002) Arch Biochem Biophys , vol.405 , pp. 95-103
    • Kroumova, A.B.1    Wagner, G.J.2    Davies, H.M.3
  • 30
    • 84858747436 scopus 로고    scopus 로고
    • Effect of cultivation parameters on the production of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) and poly (3-hydroxybutyrate-4-hydroxybutyrate-3-hydroxyvalerate) by Cupriavidus necator using waste glycerol
    • Cavalheiro, J.M., Raposo, R.S., de Almeida, M.C.M., Cesário, M.T., Sevrin, C., Grandfils, C., Da Fonseca, M., Effect of cultivation parameters on the production of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) and poly (3-hydroxybutyrate-4-hydroxybutyrate-3-hydroxyvalerate) by Cupriavidus necator using waste glycerol. Bioresour Technol 111 (2012), 391–397.
    • (2012) Bioresour Technol , vol.111 , pp. 391-397
    • Cavalheiro, J.M.1    Raposo, R.S.2    de Almeida, M.C.M.3    Cesário, M.T.4    Sevrin, C.5    Grandfils, C.6    Da Fonseca, M.7
  • 31
    • 0024682195 scopus 로고
    • Biodegradable poly (3-hydroxybutyrate-co-4-hydroxybutyrate) produced from γ-butyrolactone and butyric acid by Alcaligenes eutrophus
    • Doi, Y., Segawa, A., Kunioka, M., Biodegradable poly (3-hydroxybutyrate-co-4-hydroxybutyrate) produced from γ-butyrolactone and butyric acid by Alcaligenes eutrophus. Polym Commun (Guildford) 30 (1989), 169–171.
    • (1989) Polym Commun (Guildford) , vol.30 , pp. 169-171
    • Doi, Y.1    Segawa, A.2    Kunioka, M.3
  • 32
    • 0031549644 scopus 로고    scopus 로고
    • Production of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) in recombinant Escherichia coli grown on glucose
    • Valentin, H.E., Dennis, D., Production of poly (3-hydroxybutyrate-co-4-hydroxybutyrate) in recombinant Escherichia coli grown on glucose. J Biotechnol 58 (1997), 33–38.
    • (1997) J Biotechnol , vol.58 , pp. 33-38
    • Valentin, H.E.1    Dennis, D.2
  • 33
    • 84901348905 scopus 로고    scopus 로고
    • Synthesis of polyhydroxyalkanoates from glucose that contain medium-chain-length monomers via the reversed fatty acid β-oxidation cycle in Escherichia coli
    • By engineering the reversed fatty acid β-oxidation cycle, mcl-PHAs were synthesized in E. coli directly from glucose. The engineered E. coli produced 6.62 wt% of cell dry weight mcl-PHA heteropolymers by deleting the major thioesterases. Furthermore, recombinant E. coli synthesized 12.10 wt% of cell dry weight scl–mcl PHA copolymers.
    • Zhuang, Q., Wang, Q., Liang, Q., Qi, Q., Synthesis of polyhydroxyalkanoates from glucose that contain medium-chain-length monomers via the reversed fatty acid β-oxidation cycle in Escherichia coli. Metab Eng 24 (2014), 78–86 By engineering the reversed fatty acid β-oxidation cycle, mcl-PHAs were synthesized in E. coli directly from glucose. The engineered E. coli produced 6.62 wt% of cell dry weight mcl-PHA heteropolymers by deleting the major thioesterases. Furthermore, recombinant E. coli synthesized 12.10 wt% of cell dry weight scl–mcl PHA copolymers.
    • (2014) Metab Eng , vol.24 , pp. 78-86
    • Zhuang, Q.1    Wang, Q.2    Liang, Q.3    Qi, Q.4
  • 34
    • 85017513242 scopus 로고    scopus 로고
    • Semirational approach for ultrahigh poly(3-hydroxybutyrate) accumulation in Escherichia coli by combining one-step library construction and high-throughput screening
    • This paper reported a semirational approach for highly efficient PHB pathway optimization in E. coli based on a phbCAB operon cloned from the native producer Ralstonia entropha. Rationally designed ribosomal binding site (RBS) libraries with defined strengths for each of the three genes were constructed based on high or low copy number plasmids in a one-pot reaction by an oligo-linker mediated assembly (OLMA) method. Applying this approach, strains accumulating 0%–92% PHB contents in cell dry weight (CDW) were achieved.
    • Li, T., Ye, J., Shen, R., Zong, Y., Zhao, X., Lou, C., Chen, G.Q., Semirational approach for ultrahigh poly(3-hydroxybutyrate) accumulation in Escherichia coli by combining one-step library construction and high-throughput screening. ACS Synth Biol 5 (2016), 1308–1317 This paper reported a semirational approach for highly efficient PHB pathway optimization in E. coli based on a phbCAB operon cloned from the native producer Ralstonia entropha. Rationally designed ribosomal binding site (RBS) libraries with defined strengths for each of the three genes were constructed based on high or low copy number plasmids in a one-pot reaction by an oligo-linker mediated assembly (OLMA) method. Applying this approach, strains accumulating 0%–92% PHB contents in cell dry weight (CDW) were achieved.
    • (2016) ACS Synth Biol , vol.5 , pp. 1308-1317
    • Li, T.1    Ye, J.2    Shen, R.3    Zong, Y.4    Zhao, X.5    Lou, C.6    Chen, G.Q.7
  • 35
    • 84926645319 scopus 로고    scopus 로고
    • Application of CRISPRi for prokaryotic metabolic engineering involving multiple genes, a case study: controllable P (3HB-co-4HB) biosynthesis
    • This paper shows that CRISPRi can also be used for fine-tuning prokaryotic gene expression while simultaneously regulating multiple essential gene expression with less labor and time consumption. CRISPRi was used to control polyhydroxyalkanoate (PHA) biosynthesis pathway flux and to adjust PHA composition in this study.
    • Lv, L., Ren, Y.-L., Chen, J.-C., Wu, Q., Chen, G.-Q., Application of CRISPRi for prokaryotic metabolic engineering involving multiple genes, a case study: controllable P (3HB-co-4HB) biosynthesis. Metab Eng 29 (2015), 160–168 This paper shows that CRISPRi can also be used for fine-tuning prokaryotic gene expression while simultaneously regulating multiple essential gene expression with less labor and time consumption. CRISPRi was used to control polyhydroxyalkanoate (PHA) biosynthesis pathway flux and to adjust PHA composition in this study.
    • (2015) Metab Eng , vol.29 , pp. 160-168
    • Lv, L.1    Ren, Y.-L.2    Chen, J.-C.3    Wu, Q.4    Chen, G.-Q.5
  • 36
    • 84968755068 scopus 로고    scopus 로고
    • Morphology engineering of bacteria for bio-production
    • Jiang, X.R., Chen, G.Q., Morphology engineering of bacteria for bio-production. Biotechnol Adv 34 (2016), 435–440.
    • (2016) Biotechnol Adv , vol.34 , pp. 435-440
    • Jiang, X.R.1    Chen, G.Q.2
  • 37
    • 84928328474 scopus 로고    scopus 로고
    • Engineering the bacterial shapes for enhanced inclusion bodies accumulation
    • Jiang, X.R., Wang, H., Shen, R., Chen, G.Q., Engineering the bacterial shapes for enhanced inclusion bodies accumulation. Metab Eng 29 (2015), 227–237.
    • (2015) Metab Eng , vol.29 , pp. 227-237
    • Jiang, X.R.1    Wang, H.2    Shen, R.3    Chen, G.Q.4
  • 38
    • 84947613662 scopus 로고    scopus 로고
    • Halophiles, coming stars for industrial biotechnology
    • Yin, J., Chen, J.-C., Wu, Q., Chen, G.-Q., Halophiles, coming stars for industrial biotechnology. Biotechnol Adv 33 (2014), 1433–1442.
    • (2014) Biotechnol Adv , vol.33 , pp. 1433-1442
    • Yin, J.1    Chen, J.-C.2    Wu, Q.3    Chen, G.-Q.4
  • 39
    • 84925938688 scopus 로고    scopus 로고
    • Open and continuous fermentation: products, conditions and bioprocess economy
    • Li, T., Chen, X.B., Chen J.C., Wu, Q., Chen, G.Q., Open and continuous fermentation: products, conditions and bioprocess economy. Biotechnol J 9 (2014), 1503–1511.
    • (2014) Biotechnol J , vol.9 , pp. 1503-1511
    • Li, T.1    Chen, X.B.2    Chen J.C.3    Wu, Q.4    Chen, G.Q.5
  • 40
    • 0344604517 scopus 로고    scopus 로고
    • Biochemical and molecular characterization of a succinate semialdehyde dehydrogenase involved in the catabolism of 4-hydroxybutyric acid in Ralstonia eutropha
    • Lütke-Eversloh, T., Steinbüchel, A., Biochemical and molecular characterization of a succinate semialdehyde dehydrogenase involved in the catabolism of 4-hydroxybutyric acid in Ralstonia eutropha. FEMS Microbiol Lett 181 (1999), 63–71.
    • (1999) FEMS Microbiol Lett , vol.181 , pp. 63-71
    • Lütke-Eversloh, T.1    Steinbüchel, A.2
  • 41
    • 84946615049 scopus 로고    scopus 로고
    • Synthesis of poly (3-hydroxybutyrate-co-3-hydroxyvalerate) from unrelated carbon sources in engineered Rhodospirillum rubrum
    • 2 and CO of artificial synthesis gas (syngas) with a 3HV content of 56 mol%.
    • 2 and CO of artificial synthesis gas (syngas) with a 3HV content of 56 mol%.
    • (2015) FEMS Microbiol Lett , vol.362 , pp. fnv038
    • Heinrich, D.1    Raberg, M.2    Steinbüchel, A.3
  • 42
    • 85017124187 scopus 로고    scopus 로고
    • Engineering Halomonas species TD01 for enhanced polyhydroxyalkanoates synthesis via CRISPRi
    • Tao, W., Lv, L., Chen, G.-Q., Engineering Halomonas species TD01 for enhanced polyhydroxyalkanoates synthesis via CRISPRi. Microb Cell Fact, 16, 2017, 48.
    • (2017) Microb Cell Fact , vol.16 , pp. 48
    • Tao, W.1    Lv, L.2    Chen, G.-Q.3
  • 43
    • 84907856997 scopus 로고    scopus 로고
    • Engineering Halomonas TD01 for the low-cost production of polyhydroxyalkanoates
    • Tan, D., Wu, Q., Chen, J.C., Chen, G.Q., Engineering Halomonas TD01 for the low-cost production of polyhydroxyalkanoates. Metab Eng 26C (2014), 34–47.
    • (2014) Metab Eng , vol.26C , pp. 34-47
    • Tan, D.1    Wu, Q.2    Chen, J.C.3    Chen, G.Q.4
  • 44
    • 85011011231 scopus 로고    scopus 로고
    • Synthesis of medium-chain-length polyhydroxyalkanoate homopolymers, random copolymers, and block copolymers by an engineered strain of Pseudomonas entomophila
    • Wang, Y., Chung, A., Chen, G.Q., Synthesis of medium-chain-length polyhydroxyalkanoate homopolymers, random copolymers, and block copolymers by an engineered strain of Pseudomonas entomophila. Adv Healthc Mater, 6, 2017, 10.1002/adhm.201601017.
    • (2017) Adv Healthc Mater , vol.6
    • Wang, Y.1    Chung, A.2    Chen, G.Q.3
  • 45
    • 85027956141 scopus 로고    scopus 로고
    • CRISPRi engineering E. coli for morphology diversification
    • Clustered regularly interspaced short palindromic repeats interference, abbreviated as CRISPRi, was for the first time used to regulate expression intensities of ftsZ or/and mreB in E. coli. Combined repressions on expressions of ftsZ and mreB generated long and larger E. coli with diverse morphologies including various sizes of gourds, bars, coccus, spindles, multi-angles and ellipsoids.
    • Elhadi, D., Lv, L., Jiang, X.R., Wu, H., Chen, G.Q., CRISPRi engineering E. coli for morphology diversification. Metab Eng 38 (2016), 358–369 Clustered regularly interspaced short palindromic repeats interference, abbreviated as CRISPRi, was for the first time used to regulate expression intensities of ftsZ or/and mreB in E. coli. Combined repressions on expressions of ftsZ and mreB generated long and larger E. coli with diverse morphologies including various sizes of gourds, bars, coccus, spindles, multi-angles and ellipsoids.
    • (2016) Metab Eng , vol.38 , pp. 358-369
    • Elhadi, D.1    Lv, L.2    Jiang, X.R.3    Wu, H.4    Chen, G.Q.5
  • 46
    • 84907331475 scopus 로고    scopus 로고
    • Engineering Escherichia coli for enhanced production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in larger cellular space
    • Wang, Y., Wu, H., Jiang, X., Chen, G.Q., Engineering Escherichia coli for enhanced production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) in larger cellular space. Metab Eng 25 (2014), 183–193.
    • (2014) Metab Eng , vol.25 , pp. 183-193
    • Wang, Y.1    Wu, H.2    Jiang, X.3    Chen, G.Q.4
  • 47
    • 85029476168 scopus 로고    scopus 로고
    • Controlling cell volume for efficient PHB production by Halomonas
    • This paper developed a temperature-responsible plasmid expression system for compensated expression of relevant gene(s) in mreB or ftsZ disrupted recombinants H. campaniensis LS21, allowing mreB or ftsZ disrupted recombinants to grow normally at 30 °C in a bioreactor for 12 h so that a certain cell density can be reached, followed by 36 h cell size expansions or cell shape elongations at elevated 37 °C at which the mreB and ftsZ encoded plasmid pTKmf failed to replicate in the recombinants and thus lost themselves.
    • Jiang, X.R., Yao, Z.H., Chen, G.Q., Controlling cell volume for efficient PHB production by Halomonas. Metab Eng 44 (2017), 30–37 This paper developed a temperature-responsible plasmid expression system for compensated expression of relevant gene(s) in mreB or ftsZ disrupted recombinants H. campaniensis LS21, allowing mreB or ftsZ disrupted recombinants to grow normally at 30 °C in a bioreactor for 12 h so that a certain cell density can be reached, followed by 36 h cell size expansions or cell shape elongations at elevated 37 °C at which the mreB and ftsZ encoded plasmid pTKmf failed to replicate in the recombinants and thus lost themselves.
    • (2017) Metab Eng , vol.44 , pp. 30-37
    • Jiang, X.R.1    Yao, Z.H.2    Chen, G.Q.3
  • 48
    • 84994662320 scopus 로고    scopus 로고
    • Fundamental factors determining the molecular weight of polyhydroxyalkanoate during biosynthesis
    • Tsuge, T., Fundamental factors determining the molecular weight of polyhydroxyalkanoate during biosynthesis. Polym J 48 (2016), 1051–1057.
    • (2016) Polym J , vol.48 , pp. 1051-1057
    • Tsuge, T.1
  • 49
    • 84899530091 scopus 로고    scopus 로고
    • Current trends in polyhydroxyalkanoates (PHAs) biosynthesis: insights from the recombinant Escherichia coli
    • Leong, Y.K., Show, P.L., Ooi, C.W., Ling, T.C., Lan, J.C.W., Current trends in polyhydroxyalkanoates (PHAs) biosynthesis: insights from the recombinant Escherichia coli. J Biotechnol 180 (2014), 52–65.
    • (2014) J Biotechnol , vol.180 , pp. 52-65
    • Leong, Y.K.1    Show, P.L.2    Ooi, C.W.3    Ling, T.C.4    Lan, J.C.W.5
  • 50
    • 84901927684 scopus 로고    scopus 로고
    • Biotechnological strategies to improve production of microbial poly-(3-hydroxybutyrate): a review of recent research work
    • This paper summarized the recent trends in the bacterial production of PHB using novel fermentation strategies combined with the use of genetic engineering to improve productivity and molecular weight of PHB that could be applied for its commercial production.
    • Peña, C., Castillo, T., García, A., Millán, M., Segura, D., Biotechnological strategies to improve production of microbial poly-(3-hydroxybutyrate): a review of recent research work. Microb Biotechnol 7 (2014), 278–293 This paper summarized the recent trends in the bacterial production of PHB using novel fermentation strategies combined with the use of genetic engineering to improve productivity and molecular weight of PHB that could be applied for its commercial production.
    • (2014) Microb Biotechnol , vol.7 , pp. 278-293
    • Peña, C.1    Castillo, T.2    García, A.3    Millán, M.4    Segura, D.5
  • 51
    • 0023690652 scopus 로고
    • Cloning and expression in Escherichia coli of the Alcaligenes eutrophus H16 poly-beta-hydroxybutyrate biosynthetic pathway
    • Slater, S.C., Voige, W., Dennis, D., Cloning and expression in Escherichia coli of the Alcaligenes eutrophus H16 poly-beta-hydroxybutyrate biosynthetic pathway. J Bacteriol 170 (1988), 4431–4436.
    • (1988) J Bacteriol , vol.170 , pp. 4431-4436
    • Slater, S.C.1    Voige, W.2    Dennis, D.3
  • 53
    • 84957435727 scopus 로고    scopus 로고
    • Engineering of core promoter regions enables the construction of constitutive and inducible promoters in Halomonas sp
    • Li, T., Li, T., Ji, W., Wang, Q., Zhang, H., Chen, G.Q., Lou, C., Ouyang, Q., Engineering of core promoter regions enables the construction of constitutive and inducible promoters in Halomonas sp. Biotechnol J 11 (2016), 219–227.
    • (2016) Biotechnol J , vol.11 , pp. 219-227
    • Li, T.1    Li, T.2    Ji, W.3    Wang, Q.4    Zhang, H.5    Chen, G.Q.6    Lou, C.7    Ouyang, Q.8
  • 55
    • 84155188952 scopus 로고    scopus 로고
    • Acidophilic bacteria and archaea: acid stable biocatalysts and their potential applications
    • Sharma, A., Kawarabayasi, Y., Satyanarayana, T., Acidophilic bacteria and archaea: acid stable biocatalysts and their potential applications. Extremophiles 16 (2012), 1–19.
    • (2012) Extremophiles , vol.16 , pp. 1-19
    • Sharma, A.1    Kawarabayasi, Y.2    Satyanarayana, T.3
  • 56
    • 84956672842 scopus 로고    scopus 로고
    • Sustainable biorefining in wastewater by engineered extreme alkaliphile Bacillus marmarensis
    • Wernick, D.G., Pontrelli, S.P., Pollock, A.W., Liao, J.C., Sustainable biorefining in wastewater by engineered extreme alkaliphile Bacillus marmarensis. Scient Rep, 2016, 6.
    • (2016) Scient Rep , pp. 6
    • Wernick, D.G.1    Pontrelli, S.P.2    Pollock, A.W.3    Liao, J.C.4
  • 57
    • 77949652591 scopus 로고    scopus 로고
    • Methanotrophs: multifunctional bacteria with promising applications in environmental bioengineering
    • Jiang, H., Chen, Y., Jiang, P., Zhang, C., Smith, T.J., Murrell, J.C., Xing, X.H., Methanotrophs: multifunctional bacteria with promising applications in environmental bioengineering. Biochem Eng J 49 (2010), 277–288.
    • (2010) Biochem Eng J , vol.49 , pp. 277-288
    • Jiang, H.1    Chen, Y.2    Jiang, P.3    Zhang, C.4    Smith, T.J.5    Murrell, J.C.6    Xing, X.H.7
  • 58
    • 84990230047 scopus 로고    scopus 로고
    • Halophilic microorganism resources and their applications in industrial and environmental biotechnology
    • In this paper, the authors discussed on recent efforts on discovery and utilization of halophiles for biotechnological interest and future perspective in biotechnology.
    • Waditee-Sirisattha, R., Kageyama, H., Takabe, T., Halophilic microorganism resources and their applications in industrial and environmental biotechnology. AIMS Microbiol 2 (2016), 42–54 In this paper, the authors discussed on recent efforts on discovery and utilization of halophiles for biotechnological interest and future perspective in biotechnology.
    • (2016) AIMS Microbiol , vol.2 , pp. 42-54
    • Waditee-Sirisattha, R.1    Kageyama, H.2    Takabe, T.3
  • 59
    • 84904535022 scopus 로고    scopus 로고
    • A seawater-based open and continuous process for polyhydroxyalkanoates production by recombinant Halomonas campaniensis LS21 grown in mixed substrates
    • Yue, H., Ling, C., Yang, T., Chen, X., Chen, Y., Deng, H., Wu, Q., Chen, J., Chen, G.Q., A seawater-based open and continuous process for polyhydroxyalkanoates production by recombinant Halomonas campaniensis LS21 grown in mixed substrates. Biotechnol Biofuels 7 (2014), 108–120.
    • (2014) Biotechnol Biofuels , vol.7 , pp. 108-120
    • Yue, H.1    Ling, C.2    Yang, T.3    Chen, X.4    Chen, Y.5    Deng, H.6    Wu, Q.7    Chen, J.8    Chen, G.Q.9
  • 60
    • 85026914877 scopus 로고    scopus 로고
    • Engineering Halomonas bluephagenesis TD01 for non-sterile production of poly (3-hydroxybutyrate-co-4-hydroxybutyrate)
    • Chen, X.B., Yin, J., Ye, J., Zhang, H., Che, X., Ma, Y., Li, M., Wu, L.P., Chen, G.Q., Engineering Halomonas bluephagenesis TD01 for non-sterile production of poly (3-hydroxybutyrate-co-4-hydroxybutyrate). Bioresour Technol 244 (2017), 534–541.
    • (2017) Bioresour Technol , vol.244 , pp. 534-541
    • Chen, X.B.1    Yin, J.2    Ye, J.3    Zhang, H.4    Che, X.5    Ma, Y.6    Li, M.7    Wu, L.P.8    Chen, G.Q.9
  • 61
    • 79960839264 scopus 로고    scopus 로고
    • Unsterile and continuous production of polyhydroxybutyrate by Halomonas TD01
    • Tan, D., Xue, Y.S., Aibaidula, G., Chen, G.Q., Unsterile and continuous production of polyhydroxybutyrate by Halomonas TD01. Bioresour Technol 102 (2011), 8130–8136.
    • (2011) Bioresour Technol , vol.102 , pp. 8130-8136
    • Tan, D.1    Xue, Y.S.2    Aibaidula, G.3    Chen, G.Q.4
  • 62
    • 84920257993 scopus 로고    scopus 로고
    • Development of an enhanced chromosomal expression system based on porin synthesis operon for halophile Halomonas sp
    • Yin, J., Fu, X.Z., Wu, Q., Chen, J.C., Chen, G.Q., Development of an enhanced chromosomal expression system based on porin synthesis operon for halophile Halomonas sp. Appl Microbiol Biotechnol 98 (2014), 8987–8997.
    • (2014) Appl Microbiol Biotechnol , vol.98 , pp. 8987-8997
    • Yin, J.1    Fu, X.Z.2    Wu, Q.3    Chen, J.C.4    Chen, G.Q.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.