메뉴 건너뛰기




Volumn 551, Issue 7678, 2017, Pages 100-104

Single-cell transcriptomics reconstructs fate conversion from fibroblast to cardiomyocyte

Author keywords

[No Author keywords available]

Indexed keywords

CD200 ANTIGEN; CLCA1 PROTEIN; MEF2C PROTEIN; PROTEIN; TM4SF1 PROTEIN; TRANSCRIPTION FACTOR GATA 4; TRANSCRIPTION FACTOR TBX5; TRANSCRIPTOME; UNCLASSIFIED DRUG; VASCULAR CELL ADHESION MOLECULE 1; GATA4 PROTEIN, MOUSE; HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN; MEF2C PROTEIN, MOUSE; MESSENGER RNA; MYOCYTE ENHANCER FACTOR 2; POLYPYRIMIDINE TRACT BINDING PROTEIN; PTBP1 PROTEIN, MOUSE; T BOX TRANSCRIPTION FACTOR; T-BOX TRANSCRIPTION FACTOR 5;

EID: 85032797966     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature24454     Document Type: Article
Times cited : (155)

References (42)
  • 1
    • 77955321344 scopus 로고    scopus 로고
    • Direct reprogramming of fbroblasts into functional cardiomyocytes by defned factors
    • Ieda, M. et al. Direct reprogramming of fbroblasts into functional cardiomyocytes by defned factors. Cell 142, 375-386 (2010).
    • (2010) Cell , vol.142 , pp. 375-386
    • Ieda, M.1
  • 2
    • 84861642380 scopus 로고    scopus 로고
    • MicroRNA-mediated in vitro and in vivo direct reprogramming of cardiac fbroblasts to cardiomyocytes
    • Jayawardena, T. M. et al. microRNA-mediated in vitro and in vivo direct reprogramming of cardiac fbroblasts to cardiomyocytes. Circ. Res. 110, 1465-1473 (2012).
    • (2012) Circ. Res. , vol.110 , pp. 1465-1473
    • Jayawardena, T.M.1
  • 3
    • 84863629484 scopus 로고    scopus 로고
    • In vivo reprogramming of murine cardiac fbroblasts into induced cardiomyocytes
    • Qian, L. et al. In vivo reprogramming of murine cardiac fbroblasts into induced cardiomyocytes. Nature 485, 593-598 (2012).
    • (2012) Nature , vol.485 , pp. 593-598
    • Qian, L.1
  • 4
    • 84863626782 scopus 로고    scopus 로고
    • Heart repair by reprogramming non-myocytes with cardiac transcription factors
    • Song, K. et al. Heart repair by reprogramming non-myocytes with cardiac transcription factors. Nature 485, 599-604 (2012).
    • (2012) Nature , vol.485 , pp. 599-604
    • Song, K.1
  • 5
    • 85013472499 scopus 로고    scopus 로고
    • Demethylation of H3K27 is essential for the induction of direct cardiac reprogramming by miR combo
    • Dal-Pra, S., Hodgkinson, C. P., Mirotsou, M., Kirste, I. & Dzau, V. J. Demethylation of H3K27 is essential for the induction of direct cardiac reprogramming by miR combo. Circ. Res. 120, 1403-1413 (2017).
    • (2017) Circ. Res. , vol.120 , pp. 1403-1413
    • Dal-Pra, S.1    Hodgkinson, C.P.2    Mirotsou, M.3    Kirste, I.4    Dzau, V.J.5
  • 6
    • 84947567460 scopus 로고    scopus 로고
    • In vivo cardiac reprogramming using an optimal single polycistronic construct
    • Ma, H., Wang, L., Yin, C., Liu, J. & Qian, L. In vivo cardiac reprogramming using an optimal single polycistronic construct. Cardiovasc. Res. 108, 217-219 (2015).
    • (2015) Cardiovasc. Res. , vol.108 , pp. 217-219
    • Ma, H.1    Wang, L.2    Yin, C.3    Liu, J.4    Qian, L.5
  • 7
    • 84880316804 scopus 로고    scopus 로고
    • In vivo cardiac cellular reprogramming efcacy is enhanced by angiogenic preconditioning of the infarcted myocardium with vascular endothelial growth factor
    • Mathison, M. et al. In vivo cardiac cellular reprogramming efcacy is enhanced by angiogenic preconditioning of the infarcted myocardium with vascular endothelial growth factor. J. Am. Heart Assoc. 1, e005652 (2012).
    • (2012) J. Am. Heart Assoc. , vol.1 , pp. e005652
    • Mathison, M.1
  • 8
    • 85011006666 scopus 로고    scopus 로고
    • Chemical enhancement of in vitro and in vivo direct cardiac reprogramming
    • Mohamed, T. M. et al. Chemical enhancement of in vitro and in vivo direct cardiac reprogramming. Circulation 135, 978-995 (2017).
    • (2017) Circulation , vol.135 , pp. 978-995
    • Mohamed, T.M.1
  • 9
    • 84941595669 scopus 로고    scopus 로고
    • High-efciency reprogramming of fbroblasts into cardiomyocytes requires suppression of pro-fbrotic signalling
    • Zhao, Y. et al. High-efciency reprogramming of fbroblasts into cardiomyocytes requires suppression of pro-fbrotic signalling. Nat. Commun. 6, 8243 (2015).
    • (2015) Nat. Commun. , vol.6 , pp. 8243
    • Zhao, Y.1
  • 10
    • 84942939090 scopus 로고    scopus 로고
    • Akt1/protein kinase B enhances transcriptional reprogramming of fbroblasts to functional cardiomyocytes
    • Zhou, H., Dickson, M. E., Kim, M. S., Bassel-Duby, R. & Olson, E. N. Akt1/protein kinase B enhances transcriptional reprogramming of fbroblasts to functional cardiomyocytes. Proc. Natl Acad. Sci. USA 112, 11864-11869 (2015).
    • (2015) Proc. Natl Acad. Sci. USA , vol.112 , pp. 11864-11869
    • Zhou, H.1    Dickson, M.E.2    Kim, M.S.3    Bassel-Duby, R.4    Olson, E.N.5
  • 11
    • 84896303301 scopus 로고    scopus 로고
    • Inhibition of TGFβ signaling increases direct conversion of fbroblasts to induced cardiomyocytes
    • Ifkovits, J. L., Addis, R. C., Epstein, J. A. & Gearhart, J. D. Inhibition of TGFβ signaling increases direct conversion of fbroblasts to induced cardiomyocytes. PLoS ONE 9, e89678 (2014).
    • (2014) PLoS ONE , vol.9 , pp. e89678
    • Ifkovits, J.L.1    Addis, R.C.2    Epstein, J.A.3    Gearhart, J.D.4
  • 12
    • 84959276987 scopus 로고    scopus 로고
    • Re-patterning of H3K27me3 H3K4me3 and DNA methylation during fbroblast conversion into induced cardiomyocytes
    • Liu, Z. et al. Re-patterning of H3K27me3, H3K4me3 and DNA methylation during fbroblast conversion into induced cardiomyocytes. Stem Cell Res. 16, 507-518 (2016).
    • (2016) Stem Cell Res. , vol.16 , pp. 507-518
    • Liu, Z.1
  • 13
    • 84904580766 scopus 로고    scopus 로고
    • MiR-133 promotes cardiac reprogramming by directly repressing Snai1 and silencing fbroblast signatures
    • Muraoka, N. et al. MiR-133 promotes cardiac reprogramming by directly repressing Snai1 and silencing fbroblast signatures. EMBO J. 33, 1565-1581 (2014).
    • (2014) EMBO J. , vol.33 , pp. 1565-1581
    • Muraoka, N.1
  • 14
    • 84922105989 scopus 로고    scopus 로고
    • Stoichiometry of Gata4, Mef2c, and Tbx5 infuences the efciency and quality of induced cardiac myocyte reprogramming
    • Wang, L. et al. Stoichiometry of Gata4, Mef2c, and Tbx5 infuences the efciency and quality of induced cardiac myocyte reprogramming. Circ. Res. 116, 237-244 (2015).
    • (2015) Circ. Res. , vol.116 , pp. 237-244
    • Wang, L.1
  • 15
    • 84960112104 scopus 로고    scopus 로고
    • Bmi1 is a key epigenetic barrier to direct cardiac reprogramming
    • Zhou, Y. et al. Bmi1 is a key epigenetic barrier to direct cardiac reprogramming. Cell Stem Cell 18, 382-395 (2016).
    • (2016) Cell Stem Cell , vol.18 , pp. 382-395
    • Zhou, Y.1
  • 16
    • 84941010341 scopus 로고    scopus 로고
    • Single-cell RNA-Seq with waterfall reveals molecular cascades underlying adult neurogenesis
    • Shin, J. et al. Single-cell RNA-Seq with waterfall reveals molecular cascades underlying adult neurogenesis. Cell Stem Cell 17, 360-372 (2015).
    • (2015) Cell Stem Cell , vol.17 , pp. 360-372
    • Shin, J.1
  • 17
    • 84969505817 scopus 로고    scopus 로고
    • SLICER: Inferring branched nonlinear cellular trajectories from single cell RNA-seq data
    • Welch, J. D., Hartemink, A. J. & Prins, J. F. SLICER: inferring branched, nonlinear cellular trajectories from single cell RNA-seq data. Genome Biol. 17, 106 (2016).
    • (2016) Genome Biol. , vol.17 , pp. 106
    • Welch, J.D.1    Hartemink, A.J.2    Prins, J.F.3
  • 18
    • 84871586080 scopus 로고    scopus 로고
    • A molecular roadmap of reprogramming somatic cells into iPS cells
    • Polo, J. M. et al. A molecular roadmap of reprogramming somatic cells into iPS cells. Cell 151, 1617-1632 (2012).
    • (2012) Cell , vol.151 , pp. 1617-1632
    • Polo, J.M.1
  • 19
    • 84978910922 scopus 로고    scopus 로고
    • Generation of an inducible fbroblast cell line for studying direct cardiac reprogramming
    • Vaseghi, H. R. et al. Generation of an inducible fbroblast cell line for studying direct cardiac reprogramming. Genesis 54, 398-406 (2016).
    • (2016) Genesis , vol.54 , pp. 398-406
    • Vaseghi, H.R.1
  • 20
    • 84922255144 scopus 로고    scopus 로고
    • Context-dependent control of alternative splicing by RNA-binding proteins
    • Fu, X. D. & Ares, M. Jr. Context-dependent control of alternative splicing by RNA-binding proteins. Nat. Rev. Genet. 15, 689-701 (2014).
    • (2014) Nat. Rev. Genet. , vol.15 , pp. 689-701
    • Fu, X.D.1    Ares, M.2
  • 21
    • 84994740646 scopus 로고    scopus 로고
    • RMAPS: RNA map analysis and plotting server for alternative exon regulation
    • Park, J. W., Jung, S., Rouchka, E. C., Tseng, Y. T. & Xing, Y. rMAPS: RNA map analysis and plotting server for alternative exon regulation. Nucleic Acids Res. 44, W333-W338 (2016).
    • (2016) Nucleic Acids Res. , vol.44 , pp. W333-W338
    • Park, J.W.1    Jung, S.2    Rouchka, E.C.3    Tseng, Y.T.4    Xing, Y.5
  • 22
    • 84924629414 scopus 로고    scopus 로고
    • Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2
    • Love, M. I., Huber, W. & Anders, S. Moderated estimation of fold change and dispersion for RNA-seq data with DESeq2. Genome Biol. 15, 550 (2014).
    • (2014) Genome Biol. , vol.15 , pp. 550
    • Love, M.I.1    Huber, W.2    Anders, S.3
  • 23
    • 84949464639 scopus 로고    scopus 로고
    • Improved generation of induced cardiomyocytes using a polycistronic construct expressing optimal ratio of Gata4, Mef2c and Tbx5
    • Wang, L. et al. Improved generation of induced cardiomyocytes using a polycistronic construct expressing optimal ratio of Gata4, Mef2c and Tbx5. J. Vis. Exp. 105, e53426 (2015).
    • (2015) J. Vis. Exp. , vol.105 , pp. e53426
    • Wang, L.1
  • 24
    • 79955027617 scopus 로고    scopus 로고
    • Co-occupancy by multiple cardiac transcription factors identifes transcriptional enhancers active in heart
    • He, A., Kong, S. W., Ma, Q. & Pu, W. T. Co-occupancy by multiple cardiac transcription factors identifes transcriptional enhancers active in heart. Proc. Natl Acad. Sci. USA 108, 5632-5637 (2011).
    • (2011) Proc. Natl Acad. Sci. USA , vol.108 , pp. 5632-5637
    • He, A.1    Kong, S.W.2    Ma, Q.3    Pu, W.T.4
  • 25
    • 84958661999 scopus 로고    scopus 로고
    • The cardiac TBX5 interactome reveals a chromatin remodeling network essential for cardiac septation
    • Waldron, L. et al. The cardiac TBX5 interactome reveals a chromatin remodeling network essential for cardiac septation. Dev. Cell 36, 262-275 (2016).
    • (2016) Dev. Cell , vol.36 , pp. 262-275
    • Waldron, L.1
  • 26
    • 84903769379 scopus 로고    scopus 로고
    • Prolyl hydroxylation by EglN2 destabilizes FOXO3a by blocking its interaction with the USP9x deubiquitinase
    • Zheng, X. et al. Prolyl hydroxylation by EglN2 destabilizes FOXO3a by blocking its interaction with the USP9x deubiquitinase. Genes Dev. 28, 1429-1444 (2014).
    • (2014) Genes Dev. , vol.28 , pp. 1429-1444
    • Zheng, X.1
  • 27
    • 84879333188 scopus 로고    scopus 로고
    • Reprogramming of mouse fbroblasts into cardiomyocyte-like cells in vitro
    • Qian, L., Berry, E. C., Fu, J. D., Ieda, M. & Srivastava, D. Reprogramming of mouse fbroblasts into cardiomyocyte-like cells in vitro. Nat. Protoc. 8, 1204-1215 (2013).
    • (2013) Nat. Protoc. , vol.8 , pp. 1204-1215
    • Qian, L.1    Berry, E.C.2    Fu, J.D.3    Ieda, M.4    Srivastava, D.5
  • 28
    • 84893910301 scopus 로고    scopus 로고
    • Quantitative assessment of single-cell RNA-sequencing methods
    • Wu, A. R. et al. Quantitative assessment of single-cell RNA-sequencing methods. Nat. Methods 11, 41-46 (2014).
    • (2014) Nat. Methods , vol.11 , pp. 41-46
    • Wu, A.R.1
  • 29
    • 84900529199 scopus 로고    scopus 로고
    • Reconstructing lineage hierarchies of the distal lung epithelium using single-cell RNA-seq
    • Treutlein, B. et al. Reconstructing lineage hierarchies of the distal lung epithelium using single-cell RNA-seq. Nature 509, 371-375 (2014).
    • (2014) Nature , vol.509 , pp. 371-375
    • Treutlein, B.1
  • 30
    • 84928987900 scopus 로고    scopus 로고
    • HTSeq-a Python framework to work with high-throughput sequencing data
    • Anders, S., Pyl, P. T. & Huber, W. HTSeq-a Python framework to work with high-throughput sequencing data. Bioinformatics 31, 166-169 (2015).
    • (2015) Bioinformatics , vol.31 , pp. 166-169
    • Anders, S.1    Pyl, P.T.2    Huber, W.3
  • 31
    • 84887109584 scopus 로고    scopus 로고
    • Accounting for technical noise in single-cell RNA-seq experiments
    • Brennecke, P. et al. Accounting for technical noise in single-cell RNA-seq experiments. Nat. Methods 10, 1093-1095 (2013).
    • (2013) Nat. Methods , vol.10 , pp. 1093-1095
    • Brennecke, P.1
  • 32
    • 33845432928 scopus 로고    scopus 로고
    • Adjusting batch efects in microarray expression data using empirical Bayes methods
    • Johnson, W. E., Li, C. & Rabinovic, A. Adjusting batch efects in microarray expression data using empirical Bayes methods. Biostatistics 8, 118-127 (2007).
    • (2007) Biostatistics , vol.8 , pp. 118-127
    • Johnson, W.E.1    Li, C.2    Rabinovic, A.3
  • 33
    • 79952230540 scopus 로고    scopus 로고
    • Removing batch efects in analysis of expression microarray data: An evaluation of six batch adjustment methods
    • Chen, C. et al. Removing batch efects in analysis of expression microarray data: an evaluation of six batch adjustment methods. PLoS ONE 6, e17238 (2011).
    • (2011) PLoS ONE , vol.6 , pp. e17238
    • Chen, C.1
  • 34
    • 84878995293 scopus 로고    scopus 로고
    • MBNL proteins repress ES-cell-specifc alternative splicing and reprogramming
    • Han, H. et al. MBNL proteins repress ES-cell-specifc alternative splicing and reprogramming. Nature 498, 241-245 (2013).
    • (2013) Nature , vol.498 , pp. 241-245
    • Han, H.1
  • 35
    • 84900873950 scopus 로고    scopus 로고
    • The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells
    • Trapnell, C. et al. The dynamics and regulators of cell fate decisions are revealed by pseudotemporal ordering of single cells. Nat. Biotechnol. 32, 381-386 (2014).
    • (2014) Nat. Biotechnol. , vol.32 , pp. 381-386
    • Trapnell, C.1
  • 36
    • 84926507971 scopus 로고    scopus 로고
    • Limma powers diferential expression analyses for RNA-sequencing and microarray studies
    • Ritchie, M. E. et al. limma powers diferential expression analyses for RNA-sequencing and microarray studies. Nucleic Acids Res. 43, e47 (2015).
    • (2015) Nucleic Acids Res. , vol.43 , pp. e47
    • Ritchie, M.E.1
  • 37
    • 84919936245 scopus 로고    scopus 로고
    • RMATS: Robust and fexible detection of diferential alternative splicing from replicate RNA-seq data
    • Shen, S. et al. rMATS: robust and fexible detection of diferential alternative splicing from replicate RNA-seq data. Proc. Natl Acad. Sci. USA 111, E5593-E5601 (2014).
    • (2014) Proc. Natl Acad. Sci. USA , vol.111 , pp. E5593-E5601
    • Shen, S.1
  • 38
    • 84860997131 scopus 로고    scopus 로고
    • An abundant tissue macrophage population in the adult murine heart with a distinct alternatively-activated macrophage profle
    • Pinto, A. R. et al. An abundant tissue macrophage population in the adult murine heart with a distinct alternatively-activated macrophage profle. PLoS ONE 7, e36814 (2012).
    • (2012) PLoS ONE , vol.7 , pp. e36814
    • Pinto, A.R.1
  • 39
    • 0035985177 scopus 로고    scopus 로고
    • Identifcation of genes periodically expressed in the human cell cycle and their expression in tumors
    • Whitfeld, M. L. et al. Identifcation of genes periodically expressed in the human cell cycle and their expression in tumors. Mol. Biol. Cell 13, 1977-2000 (2002).
    • (2002) Mol. Biol. Cell , vol.13 , pp. 1977-2000
    • Whitfeld, M.L.1
  • 40
    • 60849094513 scopus 로고    scopus 로고
    • Tropomyosin exons as models for alternative splicing
    • Gooding, C. & Smith, C. W. Tropomyosin exons as models for alternative splicing. Adv. Exp. Med. Biol. 644, 27-42 (2008).
    • (2008) Adv. Exp. Med. Biol. , vol.644 , pp. 27-42
    • Gooding, C.1    Smith, C.W.2
  • 41
    • 85011940615 scopus 로고    scopus 로고
    • Conserved developmental alternative splicing of muscleblind-like (MBNL) transcripts regulates MBNL localization and activity
    • Terenzi, F. & Ladd, A. N. Conserved developmental alternative splicing of muscleblind-like (MBNL) transcripts regulates MBNL localization and activity. RNA Biol. 7, 43-55 (2010).
    • (2010) RNA Biol. , vol.7 , pp. 43-55
    • Terenzi, F.1    Ladd, A.N.2
  • 42
    • 84906079761 scopus 로고    scopus 로고
    • Translational control of tropomyosin expression in vertebrate hearts
    • Dube, D. K., McLean, M. D., Dube, S. & Poiesz, B. J. Translational control of tropomyosin expression in vertebrate hearts. Anat. Rec. (Hoboken) 297, 1585-1595 (2014).
    • (2014) Anat. Rec. (Hoboken) , vol.297 , pp. 1585-1595
    • Dube, D.K.1    McLean, M.D.2    Dube, S.3    Poiesz, B.J.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.