Akt1/protein kinase B enhances transcriptional reprogramming of fibroblasts to functional cardiomyocytes

Proceedings of the National Academy of Sciences of the United States of America

Volumn 112, Issue 38, 2015, Pages 11864-11869

  Zhou, Huanyu ^a   Dickson, Matthew E ^a   Kim, Min Soo ^a   Bassel Duby, Rhonda ^a   Olson, Eric N ^a  

^a UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

Cardiogenesis; Cardiomyopathy; Heart; Regeneration; Transdifferentiation

Indexed keywords

MAMMALIAN TARGET OF RAPAMYCIN COMPLEX 1; PHOSPHATIDYLINOSITOL 3 KINASE; PROTEIN KINASE; PROTEIN KINASE B; SOMATOMEDIN C; TRANSCRIPTION FACTOR; TRANSCRIPTION FACTOR FKHRL1; TRANSCRIPTION FACTOR GATA 4; TRANSCRIPTION FACTOR HAND2; TRANSCRIPTION FACTOR MEF2C; TRANSCRIPTION FACTOR TBX5; UNCLASSIFIED DRUG; CALCIUM;

ANIMAL CELL; ARTICLE; CALCIUM TRANSPORT; EMBRYO; FIBROBLAST; GENE EXPRESSION; HEART FIBROBLAST; HEART MUSCLE CELL; MOUSE; NONHUMAN; PHENOTYPE; PRIORITY JOURNAL; ANIMAL; CALCIUM SIGNALING; CARDIAC MUSCLE CELL; CELL DIFFERENTIATION; CYTOLOGY; GENETIC TRANSCRIPTION; HEART CONTRACTION; MAMMALIAN EMBRYO; METABOLISM; NUCLEAR REPROGRAMMING; SEQUENCE ANALYSIS; TIME FACTOR;

ANIMALS; CALCIUM; CALCIUM SIGNALING; CELL DIFFERENTIATION; CELLULAR REPROGRAMMING; EMBRYO, MAMMALIAN; FIBROBLASTS; MICE; MYOCARDIAL CONTRACTION; MYOCYTES, CARDIAC; PROTO-ONCOGENE PROTEINS C-AKT; SEQUENCE ANALYSIS, RNA; TIME FACTORS; TRANSCRIPTION FACTORS; TRANSCRIPTION, GENETIC;

EID: 84942939090     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1516237112     Document Type: Article

References (21)

1. Xin M, Olson EN, Bassel-Duby R (2013) Mending broken hearts: Cardiac development as a basis for adult heart regeneration and repair. Nat Rev Mol Cell Biol 14(8): 529-541.
2. Ieda M, et al. (2010) Direct reprogramming of fibroblasts into functional cardiomyocytes by defined factors. Cell 142(3): 375-386.
3. Inagawa K, et al. (2012) Induction of cardiomyocyte-like cells in infarct hearts by gene transfer of Gata4, Mef2c, and Tbx5. Circ Res 111(9): 1147-1156.
4. Jayawardena TM, et al. (2015) MicroRNA induced cardiac reprogramming in vivo: Evidence for mature cardiac myocytes and improved cardiac function. Circ Res 116(3): 418-424.
5. Nam YJ, et al. (2013) Reprogramming of human fibroblasts toward a cardiac fate. Proc Natl Acad Sci USA 110(14): 5588-5593.
6. Qian L, et al. (2012) In vivo reprogramming of murine cardiac fibroblasts into induced cardiomyocytes. Nature 485(7400): 593-598.
7. Song K, et al. (2012) Heart repair by reprogramming non-myocytes with cardiac transcription factors. Nature 485(7400): 599-604.
8. Später D, Hansson EM, Zangi L, Chien KR (2014) How to make a cardiomyocyte. Development 141(23): 4418-4431.
9. Sadahiro T, Yamanaka S, Ieda M (2015) Direct cardiac reprogramming: Progress and challenges in basic biology and clinical applications. Circ Res 116(8): 1378-1391.
10. Yang X, Pabon L, Murry CE (2014) Engineering adolescence: Maturation of human pluripotent stem cell-derived cardiomyocytes. Circ Res 114(3): 511-523.
11. Bock-Marquette I, et al. (2009) Thymosin beta4 mediated PKC activation is essential to initiate the embryonic coronary developmental program and epicardial progenitor cell activation in adult mice in vivo. J Mol Cell Cardiol 46(5): 728-738.
12. Furtado MB, et al. (2014) Cardiogenic genes expressed in cardiac fibroblasts contribute to heart development and repair. Circ Res 114(9): 1422-1434.
13. Condorelli G, et al. (2002) Akt induces enhanced myocardial contractility and cell size in vivo in transgenic mice. Proc Natl Acad Sci USA 99(19): 12333-12338.
14. Gude N, et al. (2006) Akt promotes increased cardiomyocyte cycling and expansion of the cardiac progenitor cell population. Circ Res 99(4): 381-388.
15. Shiojima I, Walsh K (2006) Regulation of cardiac growth and coronary angiogenesis by the Akt/PKB signaling pathway. Genes Dev 20(24): 3347-3365.
16. Nam YJ, et al. (2014) Induction of diverse cardiac cell types by reprogramming fibroblasts with cardiac transcription factors. Development 141(22): 4267-4278.
17. Boehm JS, et al. (2007) Integrative genomic approaches identify IKBKE as a breast cancer oncogene. Cell 129(6): 1065-1079.
18. Li H, Durbin R (2009) Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics 25(14): 1754-1760.
19. Anders S, Huber W (2010) Differential expression analysis for sequence count data. Genome Biol 11(10): R106.
20. Anders S, et al. (2013) Count-based differential expression analysis of RNA sequencing data using R and Bioconductor. Nat Protoc 8(9): 1765-1786.
21. Huang DW, et al. (2007) DAVID Bioinformatics Resources: Expanded annotation database and novel algorithms to better extract biology from large gene lists. Nucleic Acids Res 35(Web Server issue, Suppl 2): W169-W175.