Effect of Trichostatin A on radiation induced epithelial-mesenchymal transition in A549 cells

Biochemical and Biophysical Research Communications

Volumn 493, Issue 4, 2017, Pages 1534-1541

  Nagaraja, SunilGowda Sunnaghatta ^a   Krishnamoorthy, Vishnuvarthan ^a   Raviraj, Raghavi ^a   Paramasivam, Alagudinesh ^a   Nagarajan, Devipriya ^a  

^a SASTRA UNIVERSITY   (India)

Author keywords

Docking studies; EMT; Radiation; TGF 1; Trichostatin A

Indexed keywords

NERVE CELL ADHESION MOLECULE; PROTEIN ZO1; SMAD2 PROTEIN; SMAD3 PROTEIN; SMAD4 PROTEIN; TRANSCRIPTION FACTOR SNAIL; TRANSCRIPTION FACTOR ZEB1; TRANSFORMING GROWTH FACTOR BETA1; TRICHOSTATIN A; UVOMORULIN; VIMENTIN; HISTONE DEACETYLASE INHIBITOR; HYDROXAMIC ACID; MESSENGER RNA; RNA; SMAD PROTEIN; TRANSFORMING GROWTH FACTOR BETA; ZEB1 PROTEIN, HUMAN;

A-549 CELL LINE; ARTICLE; BINDING ENERGY; BINDING SITE; CELL STRUCTURE; CELL VIABILITY; CONTROLLED STUDY; DRUG EFFECT; DRUG EFFICACY; DRUG PROTEIN BINDING; E CADHERIN GENE; ENERGY; EPITHELIAL MESENCHYMAL TRANSITION; GENE EXPRESSION; HYDROGEN BOND; IC50; IRRADIATION; MIGRATION INHIBITION; MOLECULAR DOCKING; PRIORITY JOURNAL; PROTEIN EXPRESSION; SIGNAL TRANSDUCTION; SNAIL GENE; TGF BETA GENE; WESTERN BLOTTING; CELL MOTION; DRUG EFFECTS; GENETICS; HUMAN; LUNG NEOPLASMS; METABOLISM; RADIATION RESPONSE;

A549 CELLS; CELL MOVEMENT; EPITHELIAL-MESENCHYMAL TRANSITION; HISTONE DEACETYLASE INHIBITORS; HUMANS; HYDROXAMIC ACIDS; LUNG NEOPLASMS; RNA, MESSENGER; RNA, NEOPLASM; SIGNAL TRANSDUCTION; SMAD PROTEINS; SNAIL FAMILY TRANSCRIPTION FACTORS; TRANSFORMING GROWTH FACTOR BETA; ZINC FINGER E-BOX-BINDING HOMEOBOX 1;

EID: 85030831260     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2017.10.031     Document Type: Article

References (39)

1. Brunner, T.B., Kunz-Schughart, L.A., Grosse-Gehling, P., Baumann, M., Cancer stem cells as a predictive factor in radiotherapy. Semin. Radiat.Oncol 22 (2012), 151–174.
2. Polyak, K., Weinberg, R.A., Transitions between epithelial and mesenchymal states: acquisition of malignant and stem cell traits. Nat. Rev. Cancer 9 (2009), 265–273.
3. Kong, D., Li, Y., Wang, Z., Sarkar, F.H., Cancer stem cells and epithelial-to-mesenchymal transition (EMT)-Phenotypic cells: are they cousins or twins?. Cancers (Basel) 3 (2011), 716–729.
4. Marie-Egyptienne, D.T., Lohse, I., Hill, R.P., Cancer stem cells, the epithelial to mesenchymal transition (EMT) and radioresistance: potential role of hypoxia. Cancer Lett. 341 (2013), 63–72.
5. Kawamoto, A., Yokoe, T., Tanaka, K., Saigusa, S., Toiyama, Y., Yasuda, H., Inoue, Y., Miki, C., Kusunoki, M., Radiation induces epithelial-mesenchymal transition in colorectal cancer cells. Oncol. Rep. 27 (2012), 51–57.
6. Josson, S., Anderson, C.S., Sung, S.Y., Johnstone, P.A., Kubo, H., Hsieh, C.L., Arnold, R., Gururajan, M., Yates, C., Chung, L.W., Inhibition of ADAM9 expression induces epithelial phenotypic alterations and sensitizes human prostate cancer cells to radiation and chemotherapy. Prostate 71 (2011), 232–240.
7. Kalluri, R., Weinberg, R.A., The basics of epithelial-mesenchymal transition. J. Clin. Invest. 119 (2009), 1420–1428.
8. Chang, L., Graham, P.H., Hao, J., Ni, J., Bucci, J., Cozzi, P.J., Kearsley, J.H., Li, Y., Acquisition of epithelial-mesenchymal transition and cancer stem cell phenotypes is associated with activation of the PI3K/Akt/mTOR pathway in prostate cancer radioresistance. Cell Death Dis., 4, 2013, e875.
9. Peinado, H., Olmeda, D., Cano, A., Snail, Zeb and bHLH factors in tumour progression: an alliance against the epithelial phenotype?. Nat. Rev. Cancer 7 (2007), 415–428.
10. Rosivatz, E., Becker, I., Specht, K., Fricke, E., Luber, B., Busch, R., Hofler, H., Becker, K.F., Differential expression of the epithelial-mesenchymal transition regulators snail, SIP1, and twist in gastric cancer. Am. J. Pathol. 161 (2002), 1881–1891.
11. Korpal, M., Lee, E.S., Hu, G., Kang, Y., The miR-200 family inhibits epithelial-mesenchymal transition and cancer cell migration by direct targeting of E-cadherin transcriptional repressors ZEB1 and ZEB2. J. Biol. Chem. 283 (2008), 14910–14914.
12. Nagarajan, D., Melo, T., Deng, Z., Almeida, C., Zhao, W., ERK/GSK3beta/Snail signaling mediates radiation-induced alveolar epithelial-to-mesenchymal transition. Free Radic. Biol. Med. 52 (2012), 983–992.
13. Chen, Y., Liu, W., Wang, P., Hou, H., Liu, N., Gong, L., Wang, Y., Ji, K., Zhao, L., Wang, P., Halofuginone inhibits radiotherapy-induced epithelial-mesenchymal transition in lung cancer. Oncotarget 7 (2016), 71341–71352.
14. Reka, A.K., Kuick, R., Kurapati, H., Standiford, T.J., Omenn, G.S., Keshamouni, V.G., Identifying inhibitors of epithelial-mesenchymal transition by connectivity map-based systems approach. J. Thorac. Oncol. 6 (2011), 1784–1792.
15. Kim, H.J., Bae, S.C., Histone deacetylase inhibitors: molecular mechanisms of action and clinical trials as anti-cancer drugs. Am. J. Transl. Res. 3 (2011), 166–179.
16. Shi, Q.Q., Zuo, G.W., Feng, Z.Q., Zhao, L.C., Luo, L., You, Z.M., Li, D.Y., Xia, J., Li, J., Chen, D.L., Effect of trichostatin A on anti HepG2 liver carcinoma cells: inhibition of HDAC activity and activation of Wnt/beta-Catenin signaling. Asian pac. J. Cancer Prev. 15 (2014), 7849–7855.
17. Morris, G.M., Goodsell, D.S., Halliday, R.S., Huey, R., Hart, W.E., Belew, R.K., Automated docking using a lamarckian genetic algorithm and empirical binding free energy functions. J. Comput. Chem. 19 (1998), 1639–1662.
18. Heckman, C., Kanagasundaram, S., Cayer, M., Paige, J., Preparation of Cultured Cells for Scanning Electron Microscope. 2007.
19. Peinado, H., Ballestar, E., Esteller, M., Cano, A., Snail mediates E-cadherin repression by the recruitment of the Sin3A/histone deacetylase 1 (HDAC1)/HDAC2 complex. Mol. Cell Biol. 24 (2004), 306–319.
20. Laskowski, R.A., MacArthur, M.W., Moss, D.S., Thornton, J.M., PROCHECK: a progtamme to check the sterochemical quality of protein structure. J. Appl. Crystallogr. 26 (1993), 283–291.
21. Yamasaki, H., Sekimoto, T., Ohkubo, T., Douchi, T., Nagata, Y., Ozawa, M., Yoneda, Y., Zinc finger domain of Snail functions as a nuclear localization signal for importin beta-mediated nuclear import pathway. Genes cells. 10 (2005), 455–464.
22. Sheen, Y.Y., Kim, M.J., Park, S.A., Park, S.Y., Nam, J.S., Targeting the transforming growth factor-beta signaling in. Cancer Ther. Biomol. Ther. Seoul. 21 (2013), 323–331.
23. Nakao, A., Imamura, T., Souchelnytskyi, S., Kawabata, M., Ishisaki, A., Oeda, E., Tamaki, K., Hanai, J., Heldin, C.H., Miyazono, K., ten Dijke, P., TGF-beta receptor-mediated signalling through Smad2, Smad3 and Smad4. EMBO J. 16 (1997), 5353–5362.
24. Grimes, H.L., Chan, T.O., Zweidler-McKay, P.A., Tong, B., Tsichlis, P.N., The Gfi-1 proto-oncoprotein contains a novel transcriptional repressor domain, SNAG, and inhibits G1 arrest induced by interleukin-2 withdrawal. Mol. Cell Biol. 16 (1996), 6263–6272.
25. Thiery, J.P., Epithelial-mesenchymal transitions in development and pathologies. Curr. Opin. Cell Biol. 15 (2003), 740–746.
26. Brabletz, T., Hlubek, F., Spaderna, S., Schmalhofer, O., Hiendlmeyer, E., Jung, A., Kirchner, T., Invasion and metastasis in colorectal cancer: epithelial-mesenchymal transition, mesenchymal-epithelial transition, stem cells and beta-catenin. Cells Tissues Organs 179 (2005), 56–65.
27. Almeida, C., Nagarajan, D., Tian, J., Leal, S.W., Wheeler, K., Munley, M., Blackstock, W., Zhao, W., The role of alveolar epithelium in radiation-induced lung injury. PLoS One, 8, 2013, e53628.
28. Thiery, J.P., Acloque, H., Huang, R.Y., Nieto, M.A., Epithelial-mesenchymal transitions in development and disease. Cell 139 (2009), 871–890.
29. Huber, M.A., Kraut, N., Beug, H., Molecular requirements for epithelial-mesenchymal transition during tumor progression. Curr. Opin. Cell Biol. 17 (2005), 548–558.
30. Araki, K., Shimura, T., Suzuki, H., Tsutsumi, S., Wada, W., Yajima, T., Kobayahi, T., Kubo, N., Kuwano, H., E/N-cadherin switch mediates cancer progression via TGF-beta-induced epithelial-to-mesenchymal transition in extrahepatic cholangiocarcinoma. Br. J. Cancer 105 (2011), 1885–1893.
31. Wang, X., Xu, J., Wang, H., Wu, L., Yuan, W., Du, J., Cai, S., Trichostatin A, a histone deacetylase inhibitor, reverses epithelial-mesenchymal transition in colorectal cancer SW480 and prostate cancer PC3 cells. Biochem. Biophys. Res. Commun. 456 (2015), 320–326.
32. Kaimori, A., Potter, J.J., Choti, M., Ding, Z., Mezey, E., Koteish, A.A., Histone deacetylase inhibition suppresses the transforming growth factor beta1-induced epithelial-to-mesenchymal transition in hepatocytes. Hepatology 52 (2010), 1033–1045.
33. Yoshikawa, M., Hishikawa, K., Marumo, T., Fujita, T., Inhibition of histone deacetylase activity suppresses epithelial-to-mesenchymal transition induced by TGF-beta1 in human renal epithelial cells. J. Am. Soc. Nephrol. 18 (2007), 58–65.
34. Cano, A., Perez-Moreno, M.A., Rodrigo, I., Locascio, A., Blanco, M.J., del Barrio, M.G., Portillo, F., Nieto, M.A., The transcription factor snail controls epithelial-mesenchymal transitions by repressing E-cadherin expression. Nat. Cell Biol. 2 (2000), 76–83.
35. Bolos, V., Peinado, H., Perez-Moreno, M.A., Fraga, M.F., Esteller, M., Cano, A., The transcription factor Slug represses E-cadherin expression and induces epithelial to mesenchymal transitions: a comparison with Snail and E47 repressors. J. Cell Sci. 116 (2003), 499–511.
36. Comijn, J., Berx, G., Vermassen, P., Verschueren, K., van Grunsven, L., Bruyneel, E., Mareel, M., Huylebroeck, D., van Roy, F., The two-handed E box binding zinc finger protein SIP1 downregulates E-cadherin and induces invasion. Mol. Cell. 7 (2001), 1267–1278.
37. Park, I.H., Kang, J.H., Shin, J.M., Lee, H.M., Trichostatin a inhibits epithelial mesenchymal transition induced by TGF-beta1 in airway epithelium. PLoS One, 11, 2016, e0162058.
38. Tsuchida, K., Nakatani, M., Hitachi, K., Uezumi, A., Sunada, Y., Ageta, H., Inokuchi, K., Activin signaling as an emerging target for therapeutic interventions. Cell Commun. Signal, 7, 2009, 15.
39. Lamouille, S., Derynck, R., Cell size and invasion in TGF-beta-induced epithelial to mesenchymal transition is regulated by activation of the mTOR pathway. J. Cell. Biol. 178 (2007), 437–451.