Pathway optimization and key enzyme evolution of N-acetylneuraminate biosynthesis using an in vivo aptazyme-based biosensor

Metabolic Engineering

Volumn 43, Issue , 2017, Pages 21-28

  Yang, Peng ^a   Wang, Jing ^a   Pang, Qingxiao ^a   Zhang, Fengyu ^a   Wang, Junshu ^a   Wang, Qian ^a   Qi, Qingsheng ^a  

^a SHANDONG UNIVERSITY   (China)

Author keywords

Biosensor; Genetic selection; N acetylneuraminate; NeuAc synthase; Pathway optimization

Indexed keywords

BIOCHEMISTRY; BIOLOGY; BIOSYNTHESIS; CARBON; ENZYMES; ESCHERICHIA COLI; GLUCOSE; METABOLIC ENGINEERING;

BIOSYNTHESIS PATHWAYS; BIOSYNTHETIC PATHWAY; GENETIC SCREENING; GENETIC SELECTION; SELECTION SYSTEMS; SOLE CARBON SOURCE; STRAIN IMPROVEMENT; SYNTHASES;

BIOSENSORS;

BACTERIAL ENZYME; GLUCOSE; N ACETYLNEURAMINATE; N ACETYLNEURAMINATE SYNTHETASE; SIALIC ACID DERIVATIVE; SYNTHETASE; UNCLASSIFIED DRUG; APTAMER; ESCHERICHIA COLI PROTEIN; LYASE; N ACETYLNEURAMINIC ACID; N-ACETYLNEURAMINATE SYNTHASE;

ARTICLE; BACTERIAL GENE; BACTERIAL GROWTH; BACTERIAL STRAIN; BATCH FERMENTATION; BIOSYNTHESIS; CELL GROWTH; CONTROLLED STUDY; ENZYMIC BIOSENSOR; GENE LIBRARY; GENETIC SCREENING; GENETIC SELECTION; HIGH THROUGHPUT SCREENING; IN VIVO STUDY; METABOLIC ENGINEERING; MOLECULAR EVOLUTION; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; TETA GENE; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GENETIC PROCEDURES; GENETICS; METABOLISM;

APTAMERS, NUCLEOTIDE; BIOSENSING TECHNIQUES; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; ESCHERICHIA COLI PROTEINS; N-ACETYLNEURAMINIC ACID; OXO-ACID-LYASES;

EID: 85026764597     PISSN: 10967176     EISSN: 10967184     Source Type: Journal    
DOI: 10.1016/j.ymben.2017.08.001     Document Type: Article

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