Genome-Wide Abolishment of Mobile Genetic Elements Using Genome Shuffling and CRISPR/Cas-Assisted MAGE Allows the Efficient Stabilization of a Bacterial Chassis

ACS Synthetic Biology

Volumn 6, Issue 8, 2017, Pages 1471-1483

  Umenhoffer, Kinga ^a   Draskovits, Gábor ^a   Nyerges, Ákos ^a   Karcagi, Ildikó ^a   Bogos, Balázs ^a   Tímár, Edit ^a   Csörgö, Bálint ^a   Herczeg, Róbert ^b   Nagy, István ^a,b   Fehér, Tamás ^a   Pál, Csaba ^a   Pósfai, György ^a  

^a INSTITUTE OF EXPERIMENTAL MEDICINE   (Hungary)

^b SEQOMICS BIOTECHNOLOGY LTD   (Hungary)

Author keywords

bacterial genome reduction; CRISPR Cas assisted multiplex automated genome editing; genome shuffling; genome stabilization; mutation rate; transposon inactivation

Indexed keywords

BACTERIAL GENOME; CRISPR CAS SYSTEM; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GENETIC ENHANCEMENT; GENETICS; GENOMIC INSTABILITY; INTERSPERSED REPEAT; PROCEDURES; SITE DIRECTED MUTAGENESIS;

CRISPR-CAS SYSTEMS; DIRECTED MOLECULAR EVOLUTION; ESCHERICHIA COLI; GENETIC ENHANCEMENT; GENOME, BACTERIAL; GENOMIC INSTABILITY; INTERSPERSED REPETITIVE SEQUENCES; MUTAGENESIS, SITE-DIRECTED;

EID: 85025159683     PISSN: None     EISSN: 21615063     Source Type: Journal    
DOI: 10.1021/acssynbio.6b00378     Document Type: Article

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