Construction of a gene knockdown system based on catalytically inactive ("dead") Cas9 (dCas9) in Staphylococcus aureus

Applied and Environmental Microbiology

Volumn 83, Issue 12, 2017, Pages

  Zhao, Changlong ^a   Shu, Xueqin ^a   Sun, Baolin ^a,b  

^a UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

^b UNIVERSITY OF SCIENCE AND TECHNOLOGY OF CHINA   (China)

Author keywords

CRISPRi; DCas9; Gene knockdown; Staphylococcus aureus

Indexed keywords

BACTERIA; GENES; NUCLEIC ACIDS; RNA;

CRISPRI; DCAS9; DESIGN STRATEGIES; DNA-BINDING PROTEIN; GENE KNOCK DOWN; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS PYOGENES; TARGETING SYSTEMS;

GENE EXPRESSION;

BACTERIUM; BIOTECHNOLOGY; EUKARYOTE; GENE EXPRESSION; GENETIC ANALYSIS; GENETIC ENGINEERING; PATHOGEN; PROTEIN;

EUKARYOTA; STAPHYLOCOCCUS AUREUS; STREPTOCOCCUS PYOGENES;

BACTERIAL PROTEIN; CAS9 ENDONUCLEASE STREPTOCOCCUS PYOGENES; ENDONUCLEASE; GUIDE RNA;

DEVICES; EVALUATION STUDY; GENE SILENCING; GENETICS; METABOLISM; OPERON; PROCEDURES; STAPHYLOCOCCUS AUREUS;

BACTERIAL PROTEINS; ENDONUCLEASES; GENE KNOCKDOWN TECHNIQUES; GENE SILENCING; OPERON; RNA, GUIDE; STAPHYLOCOCCUS AUREUS;

EID: 85020014206     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00291-17     Document Type: Article

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