메뉴 건너뛰기




Volumn 90, Issue 3, 2012, Pages 214-216

Impact of normalization method on experimental outcome using RT-qPCR in Staphylococcus aureus

Author keywords

Normalization; Reference gene; RT qPCR; Staphylococcus aureus

Indexed keywords

RNA 16S;

EID: 84864101051     PISSN: 01677012     EISSN: 18728359     Source Type: Journal    
DOI: 10.1016/j.mimet.2012.05.008     Document Type: Article
Times cited : (57)

References (8)
  • 1
    • 0036668567 scopus 로고    scopus 로고
    • Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems
    • Bustin S.A. Quantification of mRNA using real-time reverse transcription PCR (RT-PCR): trends and problems. J. Mol. Endocrinol. 2002, 29:23-39.
    • (2002) J. Mol. Endocrinol. , vol.29 , pp. 23-39
    • Bustin, S.A.1
  • 3
    • 0033963304 scopus 로고    scopus 로고
    • Direct quantitative transcript analysis of the agr regulon of Staphylococcus aureus during human infection in comparison to the expression profile in vitro
    • Goerke C., Campana S., Bayer M.G., Döring G., Botzenhart K., Wolz C. Direct quantitative transcript analysis of the agr regulon of Staphylococcus aureus during human infection in comparison to the expression profile in vitro. Infect. Immun. 2000, 68:1304-1311.
    • (2000) Infect. Immun. , vol.68 , pp. 1304-1311
    • Goerke, C.1    Campana, S.2    Bayer, M.G.3    Döring, G.4    Botzenhart, K.5    Wolz, C.6
  • 4
    • 0031792579 scopus 로고    scopus 로고
    • RRNA stability in heat-killed and UV-irradiated enterotoxigenic Staphylococcus aureus and Escherichia coli O157:H7
    • McKillip J.L., Jaykus L.A., Drake M. rRNA stability in heat-killed and UV-irradiated enterotoxigenic Staphylococcus aureus and Escherichia coli O157:H7. Appl. Environ. Microbiol. 1998, 64:4264-4268.
    • (1998) Appl. Environ. Microbiol. , vol.64 , pp. 4264-4268
    • McKillip, J.L.1    Jaykus, L.A.2    Drake, M.3
  • 5
    • 33847643776 scopus 로고    scopus 로고
    • Quantification of mRNA using real-time RT-PCR
    • Nolan T., Hands R.E., Bustin S.A. Quantification of mRNA using real-time RT-PCR. Nat. Protoc. 2006, 1:1559-1582.
    • (2006) Nat. Protoc. , vol.1 , pp. 1559-1582
    • Nolan, T.1    Hands, R.E.2    Bustin, S.A.3
  • 6
    • 34547606969 scopus 로고    scopus 로고
    • Identification of suitable internal controls to study expression of a Staphylococcus aureus multidrug resistance system by quantitative real-time PCR
    • Theis T., Skurray R.A., Brown M.H. Identification of suitable internal controls to study expression of a Staphylococcus aureus multidrug resistance system by quantitative real-time PCR. J. Microbiol. Methods 2007, 70:355-362.
    • (2007) J. Microbiol. Methods , vol.70 , pp. 355-362
    • Theis, T.1    Skurray, R.A.2    Brown, M.H.3
  • 7
    • 0037129827 scopus 로고    scopus 로고
    • Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes
    • (research0034.1-research0034.11)
    • Vandesompele J., De Preter K., Pattyn F., Poppe B., Van Roy N., De Paepe A., Speleman F. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002, 3. (research0034.1-research0034.11).
    • (2002) Genome Biol. , vol.3
    • Vandesompele, J.1    De Preter, K.2    Pattyn, F.3    Poppe, B.4    Van Roy, N.5    De Paepe, A.6    Speleman, F.7
  • 8
    • 67649444944 scopus 로고    scopus 로고
    • Reference gene validation software for improved normalization
    • Caister Academic Press, London, J. Logan, K. Edwards, N. Saunders (Eds.)
    • Vandesompele J., Kubista M., Pfaffl M.W. Reference gene validation software for improved normalization. Real-Time PCR: Current Technology and Applications 2009, 47-64. Caister Academic Press, London. J. Logan, K. Edwards, N. Saunders (Eds.).
    • (2009) Real-Time PCR: Current Technology and Applications , pp. 47-64
    • Vandesompele, J.1    Kubista, M.2    Pfaffl, M.W.3


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.