An improved tetracycline-inducible expression vector for Staphylococcus aureus

Plasmid

Volumn 61, Issue 2, 2009, Pages 126-129

  Corrigan, Rebecca M ^a   Foster, Timothy J ^a  

^a TRINITY COLLEGE DUBLIN   (Ireland)

Author keywords

Controlled expression; Expression vector; Surface protein SasG; Tetracycline induction

Indexed keywords

ANHYDROTETRACYCLINE; MEMBRANE PROTEIN; SURFACE PROTEIN SASG; TETRACYCLINE; UNCLASSIFIED DRUG;

ARTICLE; BACILLUS SUBTILIS; BACTERIAL GROWTH; CONCENTRATION (PARAMETERS); EXPRESSION VECTOR; GENE CONSTRUCT; GENE EXPRESSION; GENE INDUCTION; GENE MUTATION; GENETIC VARIABILITY; MOLECULAR CLONING; NONHUMAN; PROMOTER REGION; PROTEIN EXPRESSION; STAPHYLOCOCCUS AUREUS;

BACTERIAL PROTEINS; CLONING, MOLECULAR; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC VECTORS; MEMBRANE PROTEINS; MUTATION; PROMOTER REGIONS, GENETIC; PROTEIN BIOSYNTHESIS; STAPHYLOCOCCUS AUREUS; TETRACYCLINE;

BACILLUS (BACTERIUM); BACTERIA (MICROORGANISMS); STAPHYLOCOCCUS AUREUS;

EID: 58849136154     PISSN: 0147619X     EISSN: 10959890     Source Type: Journal    
DOI: 10.1016/j.plasmid.2008.10.001     Document Type: Article

References (9)

1. Bateman B.T., Donegan N.P., Jarry T.M., Palma M., and Cheung A.L. Evaluation of a tetracycline-inducible promoter in Staphylococcus aureus in vitro and in vivo and its application in demonstrating the role of sigB in microcolony formation. Infect. Immun. 69 (2001) 7851-7857
2. Corrigan R.M., Rigby D., Handley P., and Foster T.J. The role of Staphylococcus aureus surface protein SasG in adherence and biofilm formation. Microbiology 153 (2007) 2435-2446
3. Ehrt S., Guo X.V., Hickey C.M., Ryou M., Monteleone M., Riley L.W., and Schnappinger D. Controlling gene expression in mycobacteria with anhydrotetracycline and Tet repressor. Nucleic Acids Res. 33 (2005) e21
4. Foster T.J. Molecular genetic analysis of staphylococcal virulence. Methods Microbiol. 27 (1998) 433-454
5. Horsburgh M.J., Aish J.L., White I.J., Shaw L., Lithgow J.K., and Foster S.J. SigmaB modulates virulence determinant expression and stress resistance: characterization of a functional rsbU strain derived from Staphylococcus aureus 8325-4. J. Bacteriol. 184 (2002) 5457-5467
6. Kreiswirth B.N., Lofdahl S., Betley M.J., O'Reilly M., Schlievert P.M., Bergdoll M.S., and Novick R.P. The toxic shock syndrome exotoxin structural gene is not detectably transmitted by a prophage. Nature 305 (1983) 709-712
7. Roche F.M., Massey R., Peacock S.J., Day N.P., Visai L., Speziale P., Lam A., Pallen M., and Foster T.J. Characterization of novel LPXTG-containing proteins of Staphylococcus aureus identified from genome sequences. Microbiology 149 (2003) 643-654
8. Roche F.M., Meehan M., and Foster T.J. The Staphylococcus aureus surface protein SasG and its homologues promote bacterial adherence to human desquamated nasal epithelial cells. Microbiology 149 (2003) 2759-2767
9. Zhang L., Fan F., Palmer L.M., Lonetto M.A., Petit C., Voelker L.L., St John A., Bankosky B., Rosenberg M., and McDevitt D. Regulated gene expression in Staphylococcus aureus for identifying conditional lethal phenotypes and antibiotic mode of action. Gene 255 (2000) 297-305