Inducing protein aggregation by extensional flow

Proceedings of the National Academy of Sciences of the United States of America

Volumn 114, Issue 18, 2017, Pages 4673-4678

  Dobson, John ^a   Kumar, Amit ^a   Willis, Leon F ^a   Tuma, Roman ^a   Higazi, Daniel R ^b,c   Turner, Richard ^b   Lowe, David C ^b   Ashcroft, Alison E ^a   Radford, Sheena E ^a   Kapur, Nikil ^a   Brockwell, David J ^a  

^a UNIVERSITY OF LEEDS   (United Kingdom)

^b MEDIMMUNE LTD   (United Kingdom)

^c IPSEN   (France)

Author keywords

Aggregation; Antibody; Bioprocessing; Extensional flow; Unfolding

Indexed keywords

BETA 2 MICROGLOBULIN; CYSTEINE; DISULFIDE; GRANULOCYTE COLONY STIMULATING FACTOR; MONOCLONAL ANTIBODY; MONOMER; PROTEIN; BOVINE SERUM ALBUMIN; PROTEIN AGGREGATE;

AMINO ACID SEQUENCE; ARTICLE; ATOMIC FORCE MICROSCOPY; COMPUTATIONAL FLUID DYNAMICS; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; DISPERSITY; ENERGY TRANSFER; FLOW; FLOW RATE; FLUID FLOW; FLUORESCENCE CORRELATION SPECTROSCOPY; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDRODYNAMICS; LAMINAR FLOW; PARTICLE SIZE; PHOTON CORRELATION SPECTROSCOPY; PRIORITY JOURNAL; PROTEIN AGGREGATION; SHEAR FLOW; SIZE EXCLUSION CHROMATOGRAPHY; SURFACE AREA; THERMODYNAMICS; TRANSMISSION ELECTRON MICROSCOPY; ULTRAVIOLET SPECTROPHOTOMETRY; ANIMAL; BOVINE; CHEMISTRY; HUMAN; KINETICS; PROTEIN STABILITY;

ANIMALS; ANTIBODIES, MONOCLONAL; BETA 2-MICROGLOBULIN; CATTLE; GRANULOCYTE COLONY-STIMULATING FACTOR; HUMANS; HYDRODYNAMICS; KINETICS; PROTEIN AGGREGATES; PROTEIN STABILITY; SERUM ALBUMIN, BOVINE;

EID: 85018757082     PISSN: 00278424     EISSN: 10916490     Source Type: Journal    
DOI: 10.1073/pnas.1702724114     Document Type: Article

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