Prediction of shear damage of plasmid DNA in pump and centrifuge operations using an ultra scale-down device

Biotechnology Progress

Volumn 23, Issue 4, 2007, Pages 858-865

  Zhang, Hu ^a   Kong, Simyee ^a   Booth, Andrew ^a   Boushaba, Rihab ^a   Levy, M Susana ^a   Hoare, Michael ^a  

^a UNIVERSITY COLLEGE LONDON   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

BIOMOLECULES; BIOTECHNOLOGY; CENTRIFUGAL PUMPS; PURIFICATION; SHEAR FLOW; STRAIN RATE;

FLUID STRESS; SUPERCOILED CIRCULAR (SC); THERAPEUTIC APPLICATION; ULTRA SCALE-DOWN (USD) TOOLS;

DNA;

ALCOHOL DERIVATIVE; DNA;

AGAR GEL ELECTROPHORESIS; ARTICLE; BIOTECHNOLOGY; CHEMISTRY; DNA SUPERCOILING; EQUIPMENT DESIGN; FERMENTATION; INSTRUMENTATION; MECHANICAL STRESS; METABOLISM; METHODOLOGY; MICROBIOLOGY; PLASMID; STATISTICAL MODEL; THEORETICAL MODEL; TIME;

ALCOHOLS; BIOTECHNOLOGY; DNA; DNA, SUPERHELICAL; ELECTROPHORESIS, AGAR GEL; EQUIPMENT DESIGN; FERMENTATION; INDUSTRIAL MICROBIOLOGY; MODELS, STATISTICAL; MODELS, THEORETICAL; PLASMIDS; STRESS, MECHANICAL; TIME FACTORS;

EID: 34547855540     PISSN: 87567938     EISSN: None     Source Type: Journal    
DOI: 10.1021/bp070066z     Document Type: Article

References (21)

1. 3 fermentation scale for an influenza pandemic. Biotechnol. Prog. 2005, 21, 1577-1592.
2. Newman, J. Dynamic light scattering as a probe of super helical DNA-intercalating agent interaction. Biopolymers 1984, 23, 1113-1119.
3. Carlson, A.; Signs, M.; Lierman, L.; Boor, R.; Jim Jem, K. Mechanical disruption of Escherichia coli for plasmid recovery. Biotechnol. Bioeng. 1995, 48, 303-315.
4. Tsai, J. T.; Keshavarz-Moore, E.; Ward, J.; Hoare, M.; Ayazi-Shamlou, P.; Dunnill, P. Characterisation of plasmid DNA conjugates as a basis for their processing. Bioprocess Eng. 1999, 21, 279-286.
5. Levy, M. S.; O'Kennedy, R. D.; Ayazi-Shamlou, P.; Dunnill, P. Biochemical engineering approaches to the challenges of producing pure plasmid DNA. Trends Biotechnol. 2000, 18, 296-304.
6. Boychyn, M.; Yim, S. S.; Ayazi Shamlou, P. Characterization of flow intensity in continuous centrifuges for the development of laboratory mimics. Chem. Eng. Sci. 2001, 56, 4759-4770.
7. Hutchinson, N.; Bingham, N.; Murrell, N.; Farid, S.; Hoare, M. Shear stress analysis of mammalian cell suspensions for prediction of industrial centrifugation and its verification. Biotechnol. Bioeng. 2006, 95, 483-491.
8. Virkar, P. D.; Narendranathan, T. J.; Hoare, M.; Dunnill, P. Studies of the effects of shear on globular proteins: Extension to high shear fields and to pumps. Biotechnol. Bioeng. 1981, 23, 425-429.
9. Jaouen, P.; Vandanjon, L.; Quemeneur, F. The shear stress of microalgal cell suspensions (Tetraselmis suecica) in tangential flow filtration systems: the role of pumps. Bioresour. Technol. 1999, 68, 149-154.
10. Vandanjon, L.; Rossignol, N.; Jaouen, P.; Robert, J. M.; Quemeneur, F. Effects of shear on two microalgae species. Contribution of pumps and valves in tangential flow filtration systems. Biotechnol. Bioeng. 1999, 63, 1-9.
11. Gomme, P. T.; Hunt, B. M.; Tatford, O. C.; Johnston, A.; Bertolini, J. Effect of lobe pumping on human albumin: investigating the underlying mechanisms of aggregate formation. Biotechnol. Appl. Biochem. 2006, 43, 103-111.
12. Neal, G.; Christie, J.; Keshavarz-Moore, E.; Ayazi Shamlou, P. Ultra scale-down approach for the prediction of full-scale recovery of ovine polycolonal immunoglobulins used in the manufacture of snake venom-specific Fab fragment. Biotechnol. Bioeng. 2003, 81, 149-157.
13. Boulding, N.; Yim, S. S. S.; Keshavarz-Moore, E.; Ayazi Shamlou, P.; Berry, M. Ultra scale-down to predict filtering centrifugation of secreted antibody fragments from fungal broth. Biotechnol. Bioeng. 2002, 79, 381-388.
14. Mannweiler, K.; Hoare, M. The scale-down of an industry disc-stack centrifuge. Bioprocess Eng. 1982, 8, 19-25.
15. Kay, A.; O'Kennedy, R.; Ward, J.; Keshavarz-Moore, E. Impact of plasmid size on cellular oxygen demand in Escherichia coli. Biotechnol. Appl. Biochem. 2003, 8, 1-7.
16. Birnboim, H.; Doly, J. A rapid alkaline lysis procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979, 7, 1513-1522.
17. Levy, M. S.; Collins, I. J.; Yim, S. S.; Ward, J. M.; Titchener-Hooker, N. J.; Ayazi Shamlou, P.; Dunnill, P. Effect of shear on plasmid DNA in solution. Bioprocess Eng. 1999, 20, 7-13.
18. Gomboa, J.; Olivet, A.; Iglesias, J.; Gonzalez, P. Understanding the performance of a progressive cavity pump with a metallic stator. Proceeding of the 20th International Pump User Symposium, 2003.
19. Meacle, F. J.; Zhang, H.; Papantoniou, I.; Ward, J. M.; Titchener-Hooker, N. J.; Hoare, M. Degradation of supercoiled plasmid DNA within a capillary device. Biotechnol. Bioeng. DOI: 10.1002/bit.21275
20. Bowman, R.; Davidson, N. Hydrodynamic shear breakage of DNA. Biopolymers 1972, 11, 2601-2624.
21. Virkar, P. D.; Narendranathan, T. J.; Hoare, M.; Dunnill, P. Studies of the effect of shear on globular proteins: Extension to high shear fields and to pumps. Biotechnol. Bioeng. 1981, 23, 425-429.